마우스 T세포 증식인자(interleukin-2)에 의한 마우스 T림프구의 장기배양 및 그 특성

남상윤(Sang Yun Nam),하윤문(Youn Mun Ha),최용묵(Yong Mook Choi) 大韓微生物學會 1986 大韓微生物學會誌 Vol.21 No.1

C57BL/6 마우스 모델에서 NDM 발모제의 모발성장 촉진 효과

남상윤(Sang Yoon Nam),문준환(Jun-Hwan Mun),윤영원(Young Won Yun),백인정(In Jeoung Baek),연정민(Jung Min Yon),김영철(Young Chul Kim),류광철(Kwang Chuel Ryu),이범준(Beom Jun Lee) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.1

NDM hair tonic is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate effect of NDM hair tonic on hair growth in an alopecia model of C57BL/6 mice. Hair of six-weeks old mice were removed by topical treatment of Neclean<SUP>Ⓡ</SUP>, The next day, animals were randomized and separated in groups of 6 mice. There were four experimental groups including saline (negative control), 50% ethanol (vehicle control), 3% minoxidil (MXD), and NDM tonic. The test compounds were topically treated with 0.15 ㎖ per mouse per day for 2 weeks. The hair regrowth was determined photographically and histologically and the quantity of endocrine factors, IGF-1 and TGF-β, in the skin of mice using PCR was measured. No clinical signs were found in all animals. The topical treatment of NDM tonic or MXD for 2 weeks to dorsal skin accelerated hair regrowth faster than the controls. The NDM tonic or MXD treatment also promoted hair follicle elongation compared to the controls. The NDM tonic or MXD treatment significantly increased the expression of IGF-1 in the skin of C57BL/6 mice compared to the control (p<0.05). These results suggest that NDM-tonic has hair growth promoting activities and it can be useful for treatment for baldness or alopecia.

Expression pattern of cytosolic glutathione peroxidase (cGPx) mRNA during mouse embryogenesis

남상윤(Sang-Yoon Nam),In-Jeoung Baek,Jung-Min Yon,Beom Jun Lee,Young Won Yun,Wook-Joon Yu,Jin Tae Hong,Byeongwoo Ahn,Yun-Bae Kim,Dae Joong Kim,Jong-Koo Kang 한국실험동물학회 2005 한국실험동물학회 학술발표대회 논문집 Vol.2005 No.-

The selenoprotein cytosolic glutathione peroxidase (cGPx) is ubiquitously distributed in a variety of organs, and its primary function is to protect oxidative damage. To investigate the spatial and temporal expression pattern of cGPx mRNA in embryogenesis, as this has not been studied before, reverse transcription-polymerase chain reaction (RT-PCR) was carried out in a thermal cycler using mouse-specific cGPx primers, and in situ hybridization was performed in whole embryos or embryonic tissues using digoxigenin-labeled mouse cGPx riboprobes. Expression of cGPx mRNA was detected in all the embryos retrieved from embryonic days (EDs) 7.5 to 18.5. On EDs 10.5-12.5, cGPx mRNA was highly expressed in the margin of forelimb and hindlimb buds and dorsally in the cranial neural tube, including the telencephalon, diencephalon, and hindbrain neural tube. On ED 13.5, cGPx mRNA was accumulated especially in vibrissae, forelimb and hindlimb plates, tail, and spinal cord. On EDs 14.5-16.5, cGPx mRNA was found in the developing brain, Rathke's pouch, thymus, lung, and liver. On ED 17.5, the expression of cGPx mRNA was apparent in various tissues such as brain. submandibular gland, vibrissae, heart, lung, liver, stomach, intestine, pancreas, skin, and kidney. In particular, cGPx mRNA was greatly expressed in epithelial linings and metabolically active sites such as whisker follicles, alveolar epithelium of lung, surface epithelium and glandular region of stomach, skin epithelium, and cortex and tubules of kidney. Overall results indicate that cGPx mRNA is expressed in developing embryos, cell-specifically and tissue-specifically, suggesting that cGPx may function to protect the embryo against reactive oxygen species and/or hydroperoxides massively produced by the intracellular or extracellular environment.

수종의 생약에 대한 항암효과의 실험적 연구(Ⅰ) : 백서의 자연살해세균활성에 미치는 영향

강윤호(Yun Ho Kang),김병운(Byung Woon Kim),하윤문(Youn Mun Ha),박재경(Jai Kyung Park),남상윤(Sang Yun Nam),최규철(Kyu Chul Choi),최용묵(Yong Mook Choi) 한국생약학회 1987 생약학회지 Vol.18 No.2

Natural Killer cells are considered to play an important role in antitumor immune surveillance mechanism. In this study, 21 putative anticancer drugs selected from reference were assessed by evaluating the effect on rat Natural Killer cell activity (NKCA). All 21 herb drugs were extracted in boiling water, lyophilized, autoclaved, and then used for experiment. Culture supernatant of concanavalin-A (Con-A)-stimulated rat spleen cells as a source of lymphokine was also used as a control of comparison. Rat spleen cells were used as effector and NKCA was measured in 4 hr ⁵¹Cr-release assay against Yac-1 mouse lymphoma cell line. In order to determine the optimal conditions for NKCA augmentation, effector cells were treated with 3 different concentrations of each drug for 24, or 48 hrs before testing of NKCA. In optimal conditions determined from previous results, the effect of herb drugs on NKCA were assessed in 3 to 5 experiments. NKCA was significantly enhanced by treatment with 4 herb drugs(Ponciri Fructus, Houttuyniae Herba, Aurantii Pericarpium, Nepetae Herba), Culture supernatant of Con-A-stimulated spleen cells also augmented the rat NKCA more significantly. The results show that 4 of the herb medicines supposed to display anticancer effect may have activity as a biological response modifier through augmentation of NKCA.

암컷 랫드의 생식기능에 미치는 반하 (Pinellia ternata) 추출물의 영향

김윤배(Yun-Bae Kim),채희열(Hee-Youl Chai),이기창(Ki-Chang Lee),윤영원(Young Won Yun),김대중(Dae Joong Kim),남상윤(Sang-Yoon Nam),노재섭(Jai Seup Ro),황방연(Bang Yeon Hwang),강현구(Hyun-Gu Kang) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.1

Effects of water extract of Pinellia ternata on vaginal opening, estrous cycle, blood estrogen concentrations and uterine glutathione peroxidase activity were investigated in immature and mature female rats. In pubertal onset assay, immature rats were orally administered with Pinellia ternata extract at doses of 20, 200 or 2,000 ㎎/㎏ for 20 days from day 21 of age, and sacrificed after the final administration for the morphological and enzymatic analyses in reproductive organs. During the administration period, estrous cycle was determined by vaginal cytology from the vaginal opening day. Separately, estrous cycle and daily blood concentrations of estrogen were measured from 11th day during 20-day treatment with Pinellia ternata extract in mature rats of 10 weeks old. In pubertal onset assay, vaginal opening time was somewhat delayed by the administration with Pinellia ternata at all doses used, in contrast to a marked advance in rats treated with 17β-estradiol (10 ㎍/㎏). However, Pinellia ternata increased uterine glutathione peroxidase activity which was also observed in animals treated with 17β-estradiol. Interestingly, Pinellia ternata prolonged the diestrus period in both immature and mature rats. On the contrary, 17β-estradiol arrested the estrous cycle at estrus stage in immature rats, although it prolonged diestrus-proestrus periods in mature animals. Taken together, it is suggested that the water extract of Pinellia ternata might influence the female reproductive function somewhat differently from 17β-estradiol, and that the toxicity and action mechanism of Pinellia ternata following long-term exposure remain to be clarified.

상황 배양균사체의 활성에 관한 연구(Ⅰ)

공영윤(Young Yun Kong),이관기(Kwan Ki Lee),남상윤(Sang Yun Nam),홍남두(Nam Doo Hong) 한국생약학회 1992 생약학회지 Vol.22 No.4

Phellinus linteus was examined for its anticancer activity using an animal model. Water extract of Phellinus Zinteus was prepared from artificially cultivated mycelia. Neither toxicity nor abnormal changes of hematological ` parameters were observed in the rat given orally with high doses of drug extract for 15 days. ICR mice were transplanted with Sarcoma-180 tumor cells intraperitoneally and drug extract was daily given to the mice from 1 day after tumer transplantation for 3 weeks. Administration of drug extract significantly prolonged the survival duration of Sarcoma 180-transplanted mice. For the better understanding of the anticancer activity, we have examined the effect of the drug extract administration on various killer cell functions, such as natural killer(NK) cells, cytotoxic T-lymphocytes (CTL) and macrophages which have been known to be main effector cells in immune responses against tumors. The results from the 4 hr ⁵¹Cr-release assay have shown that the drug extract augments mouse NK cell activity but neither CTL nor macrophages. It is possible, then, that the anticancer activity of the Phellinus linteus may be associated with augmentation of NK cell function in the cancerated hosts.

사람 림포카인 활성화 킬러(Lymphokine-activated killer)세포의 유도를 위한 Interleukin-2의 생산

하윤문(Youn Mun Ha),남상윤(Sang Yun Nam),박재경(Jai Kyung Park),최용묵(Yong Mook Choi) 大韓微生物學會 1989 大韓微生物學會誌 Vol.24 No.1

보양환오탕에 의한 비특이적 세포독성 T 세포 활성 증강

하종천,김영현,우원홍,남상윤,Ha, Jong-Cheon,Kim, Young-Hyun,Woo, Won-Hong,Nam, Sang-Yun 한국생약학회 2001 생약학회지 Vol.32 No.3

To explore the possible cancer therapeutic application of "Bo-yang-hwan-oh-tang" (BH), a herbal medicinal recipe used for improvement of blood stasis, we have examined its direct cytotoxicity against tumor cell, and induction of cytotoxic activity of lymphocytes. Water extract of BH alone did not exhibit direct cytotoxicity to Yac-1 target cells even with high concentrations (10 mg/ml). By exposure for 3 days, BH did not induce any nonspecific cytotoxic activity of mouse spleen cells, either, when assessed in a 4 hr $^{51}Cr-release$ assay. However, when BH was added during CD3 stimulation of non-adherent spleen cells, non-specific CTL activity was markedly promoted in a dose dependent manner. In contrast, BH did not alter activated NK cell activity following IL-2 stimulation. These data suggest that BH does not induce but upregulates non-specific CTL effecter function and that activated NK cell does not respond to BH. For elucidation of the mechanism underlying this function of BH, time kinetic study for IL-2 production using ELISA was undertaken. IL-2 production following CD3 stimulation was significantly augmented and higher level of IL-2 is sustained over 3 days in the culture medium by BH treatment. Moreover, addition of exogenous IL-2 during CD3 stimulation resulted in a similar level of cytotoxicity between control and BH-treated culture. These data indicate that the BH-mediated upregulation of non-specific CTL activity is contributed by augmentation of IL-2 production. Our data imply the possible application of BH for combination therapy of cancer with non-specific activator.

아플라톡신을 간회 투여한 랫드의 간에서 CYP450 1A1, p53의 발현과 DNA adduct의 형성

이범준,이숙진,김태명,김대중,남상윤,현상환,강종구,홍진태,김철규,윤영원,Lee, Beom Jun,Lee, Sook Jin,Kim, Tae Myoung,Kim, Dae Joong,Nam, Sang Yoon,Hyun, Sang Hwan,Kang, Jong Koo,Hong, Jin Tae,Kim, Cheul Kyu,Yun, Young Won 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4

Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus that grow in improperly stored cereals. Aflatoxin B1 ($AFB_1$) is a potent hepatocarcinogen in a variety of experimental animals including human beings. In spite of a high attention to the hepatocarcinogenecity of $AFB_1$, the relative toxicity of aflatoxins ($AFB_2$ and $AFG_1$) is not fully clarified. Sprague-Dawley male rats were orally administered with $AFB_1$, $AFB_2$, and $AFG_1$ at the dose of 250 ${\mu}g/kg$ (additionally including a dose of $1250{\mu}g/kg $ for $AFB_1$) body weight. Animals were then killed at 12, 24 or 48 hrs following aflatoxin exposure. Subsequently the immunohistochemical examination of p53, cytochrome p450 1A1 (CYP450 1A1), and glutathione-S-transferase placental form (GST-P) were performed. The level of the 8-OxodG in the liver was determined. Expressions of CYP450 1A1 and p53 were high in the liver of rats through 48 hrs after treatment of $AFB_1$ at the single dose of $250{\mu}g/kg $. This pattern was more clear as increasing doses. The treatment of $AFB_2$ and $AFG_1$ did not affect the expression of CYP450 1A1 but it caused weak expression of p53. The activity of GST were not found in the liver of rats treated with aflatoxins. The formation of 8-OxodG by $AFB_1$ increased in a dose-dependent manner up to 24 hrs after a single treatment of $AFB_1$ thereafter decreased to the level of control. The treatment of $AFB_2$ and $AFG_1$ did not affect the levels of 8-OxodG in the liver of rats with increasing time. These results in the present study indicate that $AFB_1$ among aflatoxins with low comparable levels is the most toxic as determined by early biomarkers such as CYP450 1A1, p53, GST-P, and 8-OxodG.

C57BL/6J db/db생쥐에서 여주 (Momordica Charantia)의 항당뇨 효과

정재황,이상화,허진주,이기남,남상윤,윤영원,정성훈,이영호,이범준,Jeong, Jae-Hwang,Lee, Sang-Hwa,Hue, Jin-Joo,Lee, Ki-Nam,Nam, Sang Yoon,Yun, Young Won,Jeong, Seong-woon,Lee, Young Ho,Lee, Beom Jun 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3

Many herbal extracts have been reported to have a preventive or therapeutic effect of on diabetes mellitus. Momordica Charantia commonly known as bitter melon or karela has been reported to be a medicinal plant for treating various diseases including cancers and diabetes. The objectives of this study were to investigate anti-diabetic effects of bitter melon (BM) as determined by blood glucose levels, glucose tolerance test (GTT), insulin tolerance test (ITT), insulin and HbA1C activities in serum, serum biochemical and lipid levels, histopathology, immunohistochemistry and AMPK-${\alpha}2$ expression of skeletal muscle in male C57BL/6J db/db mice. There were four experimental groups including vehicle control, BM 10 mg/kg, BM 50 mg/kg, and BM 250 mg/kg. BM at doses of 10, 50, and 250 mg/kg was orally administered to the diabetic mice everyday for 8 weeks. The treatments of BM 10, 50, and 250 mg/kg significantly decreased the blood glucose level in the diabetic mice compared with vehicle control (p < 0.05). The treatments of BM 10 and 50 mg/kg significantly decreased the GTT, ITT and HbA1c levels in the diabetic mice compared with vehicle control (p < 0.05). All BM groups significantly decreased GOT, GPT, BUN, LDL and glucose levels in the diabetic mice compared with the vehicle control mice (p < 0.05). The livers of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable decrease in the number of lipid droplets compared with the vehicle control. The pancreas of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable increase in insulin concentration of ${\beta}$-cells compared with the vehicle control. In addition, the treatments of BM 10, 50, and 250 mg/kg actually increased the expression of AMPK-${\alpha}2$ compared with vehicle control. These results suggest that BM has a respectable anti-diabetic effect resulting from inhibition of blood glucose level and lipid level in serum and that consumption of BM may give a benefit for controlling diabetes mellitus in humans.