Bisphenol A 가 송사리 , Oryzias latipes 의 수정란 부화와 어미의 산란에 미치는 영향

나오수(Oh Soo Na),백혜자(Hea Ja Baek),이영돈(Young Don Lee),오성립(Seong Rip Oh),김형배(Hyung Bae Kim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.5

내분비장애물질로 알려진 BPA가 송사리 수정란의 부화와 어미의 산란량에 미치는 영향을 번식생물학적으로 조사하였다. 송사리 수정란의 부화 소요시간은 대조구 I과 II에서 9∼12일, BPA 50㎍/ℓ 처리구에서 26∼35일, BPA 100㎍/ℓ처리구에서 26∼27일 이었으나, 200㎍/ℓ 처리구에서는 부화하지 않았다. 부화율은 대조구 I과 II에서 각각 89.8, 84.3%인데 비해, BPA 50과 100㎍/ℓ 처리구에서 각각 23.3, 22.2%로 현저하게 낮았다. 송사리 어미가 산란한 알의 수는 대조구 I과 II 그리고 BPA 50㎍/ℓ body weight 처리구에서 각각 52.5, 4.70, 48.0개인데 비해, BPA 100과, 200㎍/ℓ body weight 처리구에서 각각 21.5, 18.5개로 상대적으로 산란량이 적었다. 산란한 모든 알 중에서 비정상란의 비율은 대조구 I과 II에서 각각 11.8, 16.2% 이었고, BPA 50, 100 그리고 200㎍/ℓbody weight 처리구에서 각각 38.8, 74.1%의 비율을 보여 BPA 처리 농도가 높을수록 비정상란이 많았다. 그리고 정상란의 부화율은 대조구에서 평균 78% 이상인데 비해, BPA 처리구에서 평균 50% 이하로 대조구에 비해 상대적으로 낮았다. 이들 결과들에서 BPA 처리구는 대조구에 비해 송사리의 수정란 부화와 송사리 어미의 산란량과 난질 그리고 수정에 저해를 주는 것으로 보인다. The effects of bisphenol A (BPA), known as one of the endocrine disrupting chemicals (EDCs), on the hatching of fertilized eggs (FE) and the spawning of adult fish in songsari, Oryzias latipes were investigated. In condition of the experimental groups, incubated water temperature was 25.0±5℃ and photoperiod was 16 h (light) : 8 h (dark). FE were exposed to aqueous solutions of BPA at nominal concentration of 50, 100 and 200㎍/ℓ. The time required in hatching of FE was long in the BPA 50 and 100㎍/ℓ treatment groups when compared to the controls, and in the BPA 200㎍/ℓ treatment group, FE failed to hatch. And also hatching rate was decreased in the BPA treatment groups in comparison to the controls. Adult fishes were reared to oral administration via a diet of 50, 100 and 200㎍/ℓ body weight BPA concentration for 3 weeks. Number of total eggs spawned in the adult fish were fewer in the BPA 100 and 200 treatment groups than in the controls and BPA 50 treatment group. Frequency of abnormal eggs in the total eggs spawned was 11.8 and 16.2% in the control and acetone carrier control lower than 36.8, 46.8 and 74.1% in the BPA 50, 100 and 200 treatment groups, respectively. And hatching rate of FE decreased in the BPA treatment groups in comparison to the controls. In these results, inhibition of the hatching of FE and the quantity and quality of spawned eggs in adult fish were observed from BPA treatment groups when compared to the controls.

성스테로이드 호르몬과 고수온 처리가 조피볼락 , Sebastes schlegeli 의 성분화에 미치는 영향

이치훈(Chi Hoon Lee),나오수(Oh Soo Na),여인규(In Kyu Yeo),백혜자(Hea Ja Baek),이영돈(Young Don Lee) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.5

조피볼락, S. schlegili 치어에 estradiol-17β (E₂)와 17α-methyltestosterone (MT) 그리고 고수온을 처리하여 성분화에 미치는 영향을 조사하였다. 실험어는 출산 후 56일부터 72일까지 E₂20, 40, 60㎍/g diet 농도로 경구투여 하였고, MT는 20, 50㎍/g diet 농도로 경구투여 하였다. 고수온 처리는 대조구 그리고 호르몬 처리구와 비교하여 약 10℃ 이상인 27.0±0.5℃로 사육하였다. 출산 후 56일까지 성분화 과정에서, 생식소는 대부분 생식원세포로 구성되어 있어 성적으로 미분화 단계였다. 그러나 출산후 128일째에, 난소는 난소강과 난소박판으로 구성되어 있고, 난원세포와 주변인기난모세포는 난소박판에 분포하고 있다. 그리고 정소는 많은 정세관으로 이루어 졌고, 이들 세관내에는 정원세포들이 무리를 지어 분포하고 있고 또한 색소포들이 정소의 수질층과 기부에 드문드문 분포하고 있다. 성비에 있어, 대조구와 비교하였을 때 모든 E₂처리구는 더 많은 암컷이 관찰되었고, 반면에, 모든 MT 처리구와 고수온 처리구에서는 더 많은 수컷이 관찰되었다. 이러한 결과들에서, 성스테로이드 호르몬의 농도와 종류 그리고 고수온 사육이 조피볼락의 성분화 과정에 있어서 성결정에 영향을 주고 있다고 볼 수 있다. This study investigated the effects of estradiol-17β (E₂), 17α-methyltestosterone (MT) and high temperature (WT) on gonadal sex differentiation in black rockfish, Sebastes schlegeli. Fish were reared to oral adminstration of E₂ at nominal concentrations of 20, 40 and 60㎍/g diet, and MT at nominal concentration of 20 and 50㎍/g diet from 56 days to 77 days after parturition. In the treatment of WT, water temperature of the breeding tanks ranged 27.0±0.5℃ more than 10℃ approximately, in comparison to the control and other experimental group. In the process of sex differentiation until 56 days after parturition, gonads were composed of mostly gonia cells, sexually undifferentiated. In contrast, 128 days after parturition, the ovaries were composed of ovarian cavity and lamellae, and oogonia and perinucleolus oocytes were distributed in the lamellae of ovary, and the testes were composed of a number of seminiferous tubule, and spermatogonia were distributed in the seminiferous tubule, and also melanophore scattered in the matrix layer of testis. In the sex ratio, more females than male were observed from E₂ treatment groups when compared to the control, but more males than females were observed from MT and WT treatment groups when compared to the control. In the results of the present study, the concentration and kinds of the sex steroid hormones, and also the rearing high temperature caused to the factor of sex determination in the process of sex differentiation of black rockfish.

넙치와 범가자미 교잡종의 성장과 성현상

이영돈(Young-Don Lee),나오수(Oh Soo Na),송춘복(Choon Bok Song),노섬(Sum Rho),이화자(Hwa-Ja Lee),박준현(Choon-Hyun Park) 한국수산과학회 양식분과 1997 韓國養殖學會誌 Vol.10 No.2

스쿠티카섬모충에 감염된 자주복, Takifugu rubripes 외부증상과 피부, 아가미, 혈관내 충체의 분포

강법세 ( Bub-Se Kang ),고환봉 ( Hwan-Bong Go ),김성준 ( Sung-Jun K1m ),나오수 ( Oh-Soo Na ),이치훈 ( Chi-Hoon Lee ),김삼연 ( Sam-Yeon Kim ),이제희 ( Jehee Lee ),이영돈 ( Young-Don Lee ) 한국어병학회 2005 한국어병학회지 Vol.18 No.1

자주복, Takifugu rubripes의 내부조직과 기관에 침투한 스쿠티카섬모충의 분포

강법세 ( Bub-Se Kang ),고환봉 ( Hwan-Bong Go ),김성준 ( Sung-Jun Kim ),나오수 ( Oh-Soo Na ),이치훈 ( Chi-Hoon Lee ),김삼연 ( Sam-Yeon Kim ),이제희 ( Jehee Lee ),이영돈 ( Young-Don Lee ) 한국어병학회 2005 한국어병학회지 Vol.18 No.1

눈알고둥, Lunella coronata coreensis의 생식주기

오성립,나오수,이영돈,김형배 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

눈알고둥,Lunella coronata coreensisd의 생식주기

오성립,나오수,이영돈,김형배 제주대학교 해양연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-

We investigated reproductive cycle of the turban shell. Lunella coronata coreensis with the histological preparation of gonads and the gonadosomatic index (GSI). The turban shells were collected at the intertidal zone of Hamdeok in Jeju-Do from December. 1995 to October. 1996. The gonad was located on the surface of the liver below the stomachal caecum Posterior spiral meat part of the shell. GSI value began to increase from May as water temperature increased and reached its maximum value in August both male and female which were 56.94 and 61.88. respectively. GSI started to decrease from September thereafter. maintaining relatively low value from January to March. The reproductive cycle of L. coronata coreensis could be grouped into five successive developmental stages : multiplicative stage (female: February to April. male. April to May). growing stage (female: April to June, male: June). mature stage (female: June to August, male: July to August). ripe and spawning stave (August to September). And degenerative and recovery stage (October to March). The spawning of the turban shell occurred from August to September and the main spawning period appeared in September. The turban shell. L. coronata coreensis appeared to be gonochoristic species. Sex ratio of female to male was 1.6 : 1.0 (P > 0.05).

Formalin이 넙치(Paralichthys olivaceus) 난 발생 및 부화자어에 미치는 영향

박창범,나오수,이치훈,김병호,이영돈,허문수,이정재,정상철,이기완,노섬,송춘복,최광식,이제희,여인규,전유진 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

Effects of formalin treatment on embryogenesis and larvae growing in fertilized eggs and hatched larvae of olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solution with 100, 200, 400, 800 and 1,000 ppm of nominal formalin concentration. They were kept in sea water of 16, 18 and 22℃, respectively. Survival rate and hatchability of fertilized eggs were high in control groups more than formalin treated groups in 16, 18 and 22℃, respectively (P<0.05). A similar effect that survival rate of hatched larvae was also observed for control and treatment groups. On the other hand, fertilized eggs treated with 400ppm formalin, were all death in kept in 22℃. These results suggest that high-dose of formalin in fertilized eggs and hatched larvae in olive flounder, Paralichthys olivaceus was inhibited the normal embryogenesis for fertilized eggs and growing for hatched larvae. Also, these inhibited effects was promoted in higher temperature.

울타리 고둥, Monodonta labio의 생식주기

송영보,이치훈,나오수,이영돈 濟州大學校 海洋硏究所 2002 해양과환경연구소 연구논문집 Vol.26 No.-

1995년 9월부터 1996년 8월까지 제주대학교 해양과 환경연구소가 위치한 함덕연안 조간대에서 채집한 울타리고둥, M. labio의 생식주기를 조사하기 위하여 생식소숙도지수의 변화와 생식세포 형성과정을 조직학적방법으로 조사하였다. 1. 울타리고둥의 생식소는 패각내 나선상 육질부 하단에서 꼬리돌기까지 간장부의 표면에 위치 하였다. 생식소가 성숙하면 암컷은 짙은 녹색, 수컷은 유백색을 띠고, 방출 후에는 암컷은 연갈색, 수컷은 연황색을 나타내었다. 2. 생식소숙도지수(GSI)는 암컷과 수컷 모두 수온이 상승하는 4월부터 상승하기 시작하여 9월에 암컷 0.47, 수컷 0.42로 최고치를 보이고 이후 10월부터 급격히 감소하였다. 저수온기인 1월에 GSI는 암컷과 수컷 모두 0.07로 최저치를 보였다. 3. 생식소의 발달 단계는 분열증식기(3월∼4월), 성장기(4월∼7월), 성숙기(8월∼9월) 그리고 방출 및 회복기(10월∼1월)의 연속적인 주기로 구분 할 수 있고, 주산란 시기는 10월로 추정 된다. 4. 울타리고둥, M. labio은 자웅이체로서, 성비는 약 1:1이었다(P>0.05). Reproductive cycle of thick liped monodont. Mondonta labio was invetigated by the histological observation of gonads and the gonadosomatic index(GSI). The thick liped monodont were collected at the intertidal zone of Hamdeok in Jeju-do from September, 1995 to August, 1996. Gonad of them was located o the surface of the liver below the stomachal caecum posterior spiral meat part of the shell. GSI value began to increase from May(as water temperature increased) and reached it's maximum value in September both male and female which were 0.42 and 0.47, respectively. It began to decrease from October thereafter, maintaining relatively in low value from January to March. The reproductive cycle of this species could be classified into four successive developmental stage; multiplication stage(March to April), growing stage(April to July), mature stage(August to september), spent and recovery stage(October to January). The main spawning period of M. labio appeared in October. The top shell, M. labio appeared to be gonochorism, neither sex reversal nor hermaphroditism. The sex ratio was approximately 1.0 : 1.0(P>0.05).

Nonylphenol이 넙치, Paralichthys olivaceus의 초기 발생에 미치는 영향

문순주,김진완,나오수,김병호,이영돈,김형배,최영찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200 ㎍/L. In the control Ⅰ and Ⅱ (methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7±7.02 and 90.0±5.29% (P< 0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200 ㎍/L were reduced to 78.7±4.16, 46.0±9.17, 48.0±3.46 and 33.3±11.02% (P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape. Cumulative mortality of hatched larvae after 26 hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200 ㎍/L treatment groups, respectively. Whereas, control Ⅰ and Ⅱ were 11.7, 163%(P<0.05). Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.