Spirulina platensis의 옥외배양 최적화 및 오염생물 구제

김충재,정윤호,최강국,박용하,안치용,오희목,Kim, Choong-Jae,Jung, Yun-Ho,Choi, Gang-Guk,Park, Yong-Ha,Ahn, Chi-Yong,Oh, Hee-Mock 한국조류학회(藻類) 2006 ALGAE Vol.21 No.1

Outdoor cultivation of cyanobacterium Spirulina platensis was carried out for 40 days in a batch mode. A half concentration of the SOT based on the underground water was used as culture medium. Working volume was 5.7 tons with 0.2 m depth. During cultivation, mean water temperature, DO and light intensity were all in proper conditions for the S. platensis growth. The adjustment of pH to over 10 with Na2CO3 and addition of the 1.5% natural salt were very effective to delete contaminant organisms, Chlamydomonas moewusii and Chlorella minutissima occurred one after the other in the culture. The mean productivity of the biomass based on the dry cell weight from 14 to 25 days, after the contaminants were deleted, was 7.8 g ·m–2· d–1, which was relatively high productivity in that a half concentration of the SOT was used for the culture. Underground water used in the culture minimized contaminants invasion and addition of the 1.5% natural salt was effective to delete contaminants as well as acted as mineral supplement in outdoor cultivation of S. platensis. Harvesting using the floating activity of S. platensis was effective from mass floating in day time after overnight without agitation and illumination.

소아 발모벽에서 N-acetylcysteine 치료효과에 대한 임상적 고찰

김충재 ( Choong Jae Kim ),최훈 ( Hoon Choi ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2019 대한피부과학회지 Vol.57 No.10

Background: Trichotillomania (TTM) is a medical condition that involves recurrent and irresistible urges to pull out their own hair. TTM generally follows a chronic course in most patients and is difficult to treat and manage. Recently, N-acetylcysteine (NAC) has shown efficacy in the treatment of TTM. Objective: The purpose of this study was to assess the efficacy of treatment with NAC in trichotillomania children younger than 13 years of age. Methods: We evaluated 15 patients under 13 years old who visited the Department of Dermatology at Chosun University Hospital between 2014 and 2019 for TTM. An initial dose of 400-mg NAC was administered to patients under 8 years and 800 mg to patients older than 8 years old. If there was no improvement for 2 weeks, the dose was increased with the maximum daily being 2,400 mg. Results: The mean age of patients was 10.7 years; 8 were boys and 7 were girls. They presented with several asymptomatic focal patches of hair loss with different hair lengths on scalp. The dermoscopic findings were broken hairs, v-sign hairs, coiled hairs, and focal erythema. 10 out of 15 patients had a good response to NAC, whereas 5 patients did not respond to the treatment. The differences between the 2 groups were the treatment duration and total NAC doses. The effective group had a mean NAC of 973 mg/day for 11.5 weeks, while the non-effective group had a 1060 mg/day dose for 4 weeks. Conclusion: NAC could be an effective and safe treatment option for TTM in children. (Korean J Dermatol 2019;57(10):600∼607)

10년간 단일 기관에서의 귀 켈로이드 임상 및 역학 연구

김충재 ( Choong Jae Kim ),최훈 ( Hoon Choi ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2021 대한피부과학회지 Vol.59 No.1

Background: Keloids are benign dermal fibrous growths associated with excessive collagen deposition that usually occurs after trauma or surgery. To date, the clinical features and epidemiology of ear keloids have not been comprehensively investigated. Objective: We investigated the epidemiology and clinical characteristics of ear keloids in Koreans. Methods: We retrospectively investigated Koreans diagnosed with ear keloids by dermatologists at the Department of Dermatology, Chosun University Hospital, between January 2010 and December 2019. Results: The study included 95 patients with 120 ear keloid lesions (mean size of lesions 1.12×1.45 ㎠). Based on the ear anatomy, the keloids were observed at the following sites: the helix in 61 (50.8%) patients, the earlobe in 37 (30.8%) patients, antihelix in 10 (8.3%) patients, scapha in 3 (2.5%) patients, and the postauricular region in 9 (7.5%) patients. Among the keloids evaluated, 67 (55.8%), 36 (30.0%), 9 (7.5%), and 8 (6.7%) were categorized as lobular, dumb-bell, button, and wrap-around types, respectively. Most (75%) keloids were located on the posterior surface of the ears. Notably, 26 patients had a family history of keloids, which was significantly correlated with the development of keloids at other sites (p<0.001). Conclusion: The helix was the most common site of involvement, and lobular keloids represented the most common variety of keloids observed in this study. Keloids occurred most frequently on the posterior surface of the ears and were bigger at these sites. Patients with a family history of keloids were significantly more likely to develop keloids in areas other than the ears. (Korean J Dermatol 2021;59(1):8∼14)

생장 및 부상성이 향상된 남세균 돌연변이 균주 Arthrospira platensis M20CJK3

유찬,김충재,최강국,안치용,최종순,오희목,Yoo, Chan,Kim, Choong-Jae,Choi, Gang-Guk,Ahn, Chi-Yong,Choi, Jong-Soon,Oh, Hee-Mock 한국미생물학회 2009 미생물학회지 Vol.45 No.3

A photosynthetic cyanobacterium Arthrospira platensis, well known for health food supplement, was studied as a target species for atmospheric $CO_2$ removal as well as biomass production. Although the biomass of A. platensis was massively produced in many countries, the recovery cost of its biomass is still high. The purpose of this study was to develop the A. platensis mutant strains which have enhanced growth rate and floatation activity to reduce the recovery cost. A. platensis KCTC AG20590 was treated with 0.24% ethyl methanesulfonate (EMS) for 20 min at room temperature. The mutant strain A. platensis M20CJK3 was finally selected by its morphological and physiological features. The morphology of the mutant A. platensis M20CJK3 was changed from loose-coiled form to tight-coiled form showing short pitch. The growth and $CO_2$ uptake rate of A. platensis M20CJK3 were improved about 15% and 17% compared with A. platensis KCTC AG20590, respectively. The floatation activity of A. platensis M20CJK3 was enhanced in 2-fold compared with that of A. platensis KCTC AG20590. Soluble proteins extracted from two strains were analyzed by two dimensional electrophoresis (2-DE) and MALDI-TOF MS/MS. Among 15 protein spots induced in 2-DE analysis, two spots were the proteins related to photosynthesis and electron transfer system of the other cyanobacteria. As a consequence, it seems that the tight-coiled mutant A. platensis M20CJK3 has an advantage of high growth rate and floatation activity which are beneficial for the mass cultivation and recovery. 건강보조식품 등으로 이용되는 Arthrospira platensis는 세계적으로 대량생산되고 있으나 생산공정 중 수확단계에서 많은 비용이 소요된다. 본 연구에서는 부상을 이용한 효과적인 수확을 위해 균주의 개량을 시도하였으며, 개량균주의 생리적 물리적 특성을 파악하고자 하였다. Ethyl methanesulfonate (EMS)를 모균주 A. platensis KCTC AG20590에 0.24%의 농도로 10, 20, 30분씩 처리하여, 형태 및 부상성이 우수한 균주 A. platensis M20CJK3 균주를 분리하였다. A. platensis M20CJK3은 느슨한 형태에서 촘촘한 형태로 세포사(trichome)의 길이 및 코일간 간격이 감소하였으며, 생장 및 $CO_2$ 고정능이 각각 15%, 17% 향상되었다. 또한, 개량균주의 부상성은 모균주에 비해 2배 이상 향상되었다. 이차원 전기영동 분석을 통해 모균주와 개량균주의 단백질 발현양상을 비교분석한 결과 광합성 관련 색소의 구조와 광전자전달계에 관련된 단백질의 발현 양상이 차이를 보였다. 본 연구에서 개발된 A. platensis M20CJK3은 고밀도 대량배양 및 수확에 유리하며, A. platensis 유전자 연구의 유용한 모델 균주로 활용될 수 있을 것으로 사료된다.

N-acetylcysteine으로 치료한 소아 발모벽 2예

최훈 ( Hoon Choi ),김충재 ( Choong Jae Kim ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2020 大韓皮膚科學會誌 Vol.58 No.1

Trichotillomania (TTM) is a medical condition characterized by an intense urge to pull out of own hair, resulting in a perceptible hair loss pattern. TTM is associated with other psychiatric processes such as obsessive-compulsive disorder and bipolar disorder. TTM generally has a chronic course in most patients and is difficult to treat and manage. There are a few options for treatment in children, and the clinical response is not satisfactory. Recently, N-acetylcysteine (NAC), a glutamate modulator, has shown efficacy in the treatment of TTM and other compulsive behaviors, and it is considered a new alternative therapy in the management of TTM. Here, we present two cases of TTM in children that were successfully treated with NAC. NAC could be an effective and safe treatment option for TTM. (Korean J Dermatol 2020;58(1):47∼50)

치은 농양 기원의 피부누공 2예

김민성 ( Min Sung Kim ),김충재 ( Choong Jae Kim ),최훈 ( Hoon Choi ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ) 대한피부과학회 2019 大韓皮膚科學會誌 Vol.57 No.10

A cutaneous sinus tract or cutaneous fistula of dental origin occurs when purulent products of dental pulp necrosis spread along the path of least resistance from the root apex to the skin on the face. As many patients present with only skin problems and without toothache, it is frequently misdiagnosed as a cyst, abscess, pyogenic granuloma, actinomycosis, squamous cell carcinomas, and so on. Incorrect diagnoses lead to invasive procedures and long-term antibiotic therapy. We present 2 cases of recurrent suppurative facial dimpled nodules. Both of the cases were referred to the maxillofacial department, where the non-restorable teeth were extracted, and root canal therapy was performed. Lesions may persist for long periods before the correct diagnosis is made and the odontogenic source is treated appropriately. With early correct diagnosis, patients can be protected from unnecessary and ineffective antibiotic therapy or surgical treatment. Herein, we report 2 cases of a cutaneous sinus tract of dental origin. (Korean J Dermatol 2019;57(10):621∼625)

잔물집구진의 색소침착양진 2예

최훈 ( Hoon Choi ),김충재 ( Choong Jae Kim ),나찬호 ( Chan Ho Na ),신봉석 ( Bong Seok Shin ),김민성 ( Min Sung Kim ) 대한피부과학회 2020 대한피부과학회지 Vol.58 No.9

Prurigo pigmentosa is a rare inflammatory disease of the skin characterized by the unexpected onset of diffuse erythematous papules and macules on the chest, neck, and back, that usually resolves leaving reticular hyperpigmentation behind. Rarely, vesicular or bullous forms have been reported. We present two cases of vesiculopapular prurigo pigmentosa in two women. Two 20-year-old women presented with multiple, pruritic, erythematous vesicles and papules of various sizes on their backs for 4 months and 3 weeks, respectively. One remembered having a similar vesicular eruption 2 years ago. Histological study and clinical findings showed a vesiculopapular prurigo pigmentosa. Treatment with doxycycline 200 mg/day and topical steroid lotion showed good responses. The lesions resolved after leaving a light brown reticulated hyperpigmentation behind. Herein, we report two cases of vesiculopapular prurigo pigmentosa successfully treated with doxycycline and topical steroid lotion. (Korean J Dermatol 2020;58(9):620∼623)

그린 동계올림픽 기반조성을 위한 해수열 이용 연구

김현주(Hyeon-Ju Kim),문덕수(Deok-Su Moon),정동호(Dong-Ho Jung),이호생(Ho-Saeng Lee),전만식(Man-Sik Chun),이봉희(Bonghee Lee),김충재(Choong-Jae Kim) 한국해양환경·에너지학회 2011 한국해양환경공학회 학술대회논문집 Vol.2011 No.11

고환 부종에 발생한 미세낭 림프관기형 1예

최훈 ( Hoon Choi ),김충재 ( Choong Jae Kim ),나찬호 ( Chan Ho Na ),김민성 ( Min Sung Kim ),신봉석 ( Bong Seok Shin ) 대한피부과학회 2020 大韓皮膚科學會誌 Vol.58 No.4

광주ㆍ전남 지역의 소아ㆍ청소년 대상포진에 대한 임상적 비교 및 혈청학적 의의에 대한 연구

나찬호 ( Chan Ho Na ),김충재 ( Choong Jae Kim ),신봉석 ( Bong Seok Shin ),최훈 ( Hoon Choi ),김민성 ( Min Sung Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.9

Background: Herpes zoster is a common dermatologic disorder. However, it rarely occurs in children and adolescents in Gwangju-Jeonnam province. Objective: This study aimed to analyze the epidemiological features and clinical characteristics of herpes zoster in children and adolescents. Methods: A retrospective survey was conducted on patients who visited the department of dermatology of Chosun University Hospital in Gwangju-Jeonnam province within the past 8 years. The medical records of 103 patients aged <18 years were reviewed. We analyzed for age, gender, accompanying symptoms, dermatomal distribution, underlying disease, treatment, and complications with serologic test. Results: The male-to-female ratio of the participants was 1.08:1, and their mean age was 13.0 years. Underlying diseases were observed in 3% (3/102) of the patients. The most common dermatomal distribution was thoracic dermatome (34%), followed by trigeminal (26%), cervical (20%), lumbar (15%), and non-skin (6%). The most common accompanying symptoms were headache (10%), fever (3%), and myalgia (3%). No difference was observed between patients who were varicella-zoster virus (VZV) IgM-positive and those who were VZV IgM-negative in terms of dermatome, visual analogue scale (VAS), severity, and body mass index (BMI). Conclusion: In children and adolescents with herpes zoster, the gender ratio and dermatomal distribution were similar to those previously reported, except for the low rate of underlying diseases. The incidence of herpes zoster in children was not significantly associated with immunosuppression and underlying diseases. Higher VZV IgM titer was not associated with dermatomal distribution, higher VAS score, or BMI. This study first compared the serological test results of children. (Korean J Dermatol 2018;56(9):531∼538)