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부유형 해양 광생물반응기를 이용한 자외선과 초기 미세조류 접종 농도와의 상관관계 규명
김지훈,박한울,정성균,김수권,김희윤,박용성,홍한마루,이철균,Kim, Z-Hun,Park, Hanwool,Jung, Seong-Gyun,Kim, Su-Kwon,Kim, Hee-Yun,Park, Yong Sung,Hong, Han Ma Roo,Lee, Choul-Gyun 한국해양바이오학회 2015 한국해양바이오학회지 Vol.7 No.2
The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off film were placed in an outdoor rectangular tank containing 200 L of water. At the lower ICDs, 0.01 and 0.05 g/L, biomass productivities in the PBRs without UV cut-off film decreased by $278{\pm}21%$ and $222{\pm}3%$ compared with those with the film, respectively. In contrast, the presence of UV cut-off film did not have a significant effect on biomass productivities at the higher ICDs, 0.25 and 1.25 g/L. When the differences in biomass productivity made by the UV cut-off film were plotted against the sum of cell projection area per light receiving area of the PBR, the results revealed that the inhibitory effect of UV on biomass productivity can be negligible when the sum of cell projection area is equal to the light receiving area of the PBR. These results show that photoinhibition caused by UV radiation could be eliminated via operating the PBR with a proper ICD.
발광다이오드를 이용한 광파장에 따른 Chlorella vulgaris의 생장과 지방산 생산에 미치는 효과
김지훈(Z-Hun Kim),김동건(Dong Keun Kim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2016 한국해양바이오학회지 Vol.8 No.1
Microalgae are considered as superior biodiesel producers, because they could effectively produce high amount of lipid with fast growth rate. In this study, Chlorella vulgaris was exposed to various light wavelengths (λ<SUB>max</SUB> 470 nm, λ<SUB>max</SUB> 525 nm, and λ<SUB>max</SUB> 660 nm) using light emitting diodes (LEDs) to examine effect of light quality on their growth and fatty acid production in 0.4-L bubble column photobioreactors. Fluorescent lamps were also used as polychromatic light sources (control). From the results, biomass productivity was varied by light wavelength from 0.05 g/L/day to 0.30 g/L/day. Maximum biomass productivity was obtained from red LED among tested ones. We also observed that contents of oleic acid and linolenic acid, which affect biodiesel properties, were significantly changed depending on supplied wavelength. These results indicated that production of algal biomass, and fatty acid content and productivity could be improved or controlled by supplying specific light wavelength.
In vitro 실험을 통한 벼세포 추출물의 창상 치유 효능 평가
김지훈 ( Z Hun Kim ),김선미 ( Sun Mi Kim ),박진호 ( Jin Ho Park ),박찬미 ( Chan Mi Park ),최홍열 ( Hong Yeol Choi ),이후민 ( Hoomin Lee ),박제권 ( Jae Kweon Park ),권순조 ( Soonjo Kwon ),김동일 ( Dong Il Kim ),장규호 ( Kyu Ho Chan 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.3
본 연구에서는 에탄올과 고압 열수 추출에 의해 준비된 벼세포 추출물의 창상 치유 효능 평가를 위해, 인간 섬유아세포와 각질세포에 추출물 처리하여 세포 독성, 세포 이동능, collagen 단백질 및 유전자의 발현 양상에 대해 실험을 진행하였다. 그 결과 추출물은 처리한 범위에서 두 세포에 전혀 독성을 나타내지 않았으며, 대조구에 비해 증식을 최대 25% 촉진시키는 것으로 나타났다. 또한 인위적으로 세포에 창상을 낸 후 세포 이동능을 평가하였을 때, 비처리군에 비해 처리 농도에 비례하여 230-450% (섬유아세포), 170-350%(각질세포) 이동성이 향상되는 것을 확인할 수 있었다. 창상 치유의 중요한 역할을 하는 collagen의 유전자와 단백질 모두 추출물 처리시 농도의존적으로 증가하는 것을 확인할 수 있었다. 특히 에탄올 추출물 100 mg/ml 처리시에는 양성 대조군인 vitamin C (500 μM)와 유사한 효능을 나타내었다. 따라서 본 연구의 결과는 벼세포 추출물이 창상 치유제로서의 활용 가능성에 대해 제시하고 있다. In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibroblasts and keratinocytes. The effects of the RCEs (at 25-100 μg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cytotoxic to the two cell types, instead increasing their proliferation by up to 25% in a dose-dependent manner compared with the controls. Furthermore, both RCEs significantly enhanced the migratory ability of the two cell types (fibroblast, 230-450%; keratinocyte, 170 350%). Additionally, we examined the effect of the RCEs on type I collagen synthesis, which is important in the wound reconstruction process. The RCEs significantly increased collagen type I mRNA and protein levels to a degree comparable to that induced by vitamin C. These results suggest the RCEs to be candidate materials for use in promoting wound healing, through their actions of increasing cell migration and accelerating wound re-epithelialization.
부유형 해양 광생물반응기의 선택적 투과막의 술폰화 반응을 통한 Biofouling 억제 및 미세조류 생산성 향상
김광민(Kwangmin Kim),이윤우(Yunwoo Lee),김지훈(Z-Hun Kim),박한울(Hanwool Park),정인재(Injae Jung),박재훈(Jaehoon Park),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2017 한국해양바이오학회지 Vol.9 No.1
The purpose of this study was to inhibit biofouling on a selectively permeable membrane (SPM) and increase biomass productivity in marine photobioreactors (PBRs) for microalgal cultivation by chemical treatment. Surfaces of a SPM, composed of polyethylene terephthalate (PET), was sulfonated to decrease hydrophobicity through attaching negatively charged sulfonic groups. Reaction time of sulfonation was varied from 0 min to 60 min. As the reaction time increased, the water contact angle value of SPM surface was decreased from 75.5o to 44.5o, indicating decrease of surface hydrophobicity. Furthermore, the water permeability of sulfonated SPM was increased from 5.42 mL/㎡/s to 10.58 mL/㎡/s, which reflects higher nutrients transfer rates through the membranes, due to decreased hydrophobicity. When cultivating Tetraselmis sp. using 100-mL floating PBRs with sulfonated SPMs, biomass productivity was improved by 34% compared with the control group (non-reacted SPMs). In addition, scanning electron microscopic observation of SPMs used for cultivation clearly revealed lower degree of cell attachment on the sulfonated SPMs. These results suggest that sulfornation of a PET SPM could improve microalgal biomass productivity by increasing nutrients transfer rates and inhibiting biofouling by algal cells.
폴리비닐클로라이드 해양광생물반응기와 고밀도 폴리에틸렌 해양광생물반응기에서 미세조류, Tetraselmis sp. KCTC12236BP의 생산성 비교
정성균(Seung-Gyun Jung),김수권(Su-Kwon Kim),변문섭(Moon-Sup Bun),조용희(Yonghee Cho),신동우(Dong-Woo Shin),김지훈(Z-Hun Kim),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2016 한국해양바이오학회지 Vol.8 No.1
It is important to design photobioreactor by cheap material for economical microalgal biomass production. In this study, two types of marine photobioreactors (MPBR), made by either polyvinyl chloride (MPBR-PVC) or high density poly ethylene (MPBR-HDPE), are used and performance of these were compared. Tetraselmis sp. KCTC 12236BP is a green marine alga that isolated from Ganghwa Island, Korea, and the strain was used for marine cultivations using MPBR-PVC and MPBR-HDPE. The cultivations were performed three times in the spring season of 2012 using MPBR-PVC and of 2013 using MPBR-HDPE in the coastal area of Young Heung Island. As the results, MPBR-PVC shows higher biomass productivities than MPBR-HDPE, due to its high light transmittance. In the cultivations using MPBR-PVC, the average sea water temperature was 11.5°C during the first experiment and 16.5°C during the second and third experiments. Average light intensities during three times for experiments were 407.5, 268.1 and 273.0 μ·E·m<SUP>-2</SUP>·s<SUP>-1</SUP>, respectively. The maximum fresh cell weight and average biomass productivity were 1.2 g·L<SUP>-1</SUP> and 0.12 g·L<SUP>-1</SUP>·day<SUP>-1</SUP>. These results showed that Tetraselmis sp. KCTC12236BP were adapted well with the environmental conditions from ocean, and grow in the MPBR-PVC and MPBR-HDPE.
Chlorella miniata 의 고농도 배양을 위한 배양조건의 통계학적 최적화
염서희(Suh Hee Yeom),김소희(So Hee Kim),박하영(Ha Young Park),김지훈(Z-hun Kim),남현주(Hyun Ju Nam),이창수(Chang Soo Lee),김진우(Jin Woo Kim) 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.4
본 연구는 국립낙동강생물자원관 생물자원은행에서 분양받은 담수 미세조류인 Chlorella miniata 의 세포 성장과 루테인 생성 증진을 위해 배양 조건을 반응 표면 분석법을 이용하여 통계학적 최적화를 수행하였다. 배양 조건의 주요 독립변수는 폭기(0.03 ~ 0.18 v.v.m.), 광도(0.0 ~ 800.0 μmol/m<SUP>2</SUP>/s)와 온도(18.0 ~ 28.0°C)로 설정하였으며 배양 조건이 세포 성장에 미치는 영향을 예측하기 위해 17개 실험조건을 설계하고 실험 결과를 바탕으로 2차 회귀 모델을 도출하였다. 배양 최적 조건은 0.13 v.v.m., 595.1 μmol/m<SUP>2</SUP>/s, 25.8 °C로 확인되었으며 최적 조건에서 42시간 배양 시 3.29 (OD680)로 세포가 최대로 성장하였다. 배양 최적 조건 도출 후 최적 배양 조건에서 성장된 C. miniata 의 루테인 함량을 HPLC를 이용하여 평가하였을 때 세포 농도 증가와 함께 루테인 생산성이 25 % 이상 증가함이 확인되어 통계학적 최적화를 통해 세포성장이 효과적으로 증가되고 결과적으로 루테인 생산 증가에 기여하였다. In this study, culture conditions were optimized using response surface analysis to enhance the cell growth and lutein production of Chlorella miniata, a freshwater microalgae received at the freshwater bioresources culture collection of the Nakdonggang National Institute of Biological Resources. The main independent variables selected for the culture conditions were aeration (0.03 ~ 0.18 v.v.m.), light intensity (0.0 ~ 800.0 μmol/m<SUP>2</SUP>/s), and temperature (18.0 ~ 28.0 ℃). To predict the effect of culture conditions on cell growth, second-order regression models were derived based on the results of 17 experimental sets. The maximum cell growth of 3.29 (OD680) was obtained at 42hr under 0.13 v.v.m., 595.1 μmol/m<SUP>2</SUP>/s, and 25.8°C. C. miniata was subsequently cultured under the optimal culture conditions and the lutein content was evaluated using HPLC. Increased cell growth was observed under optimal culture conditions, and lutein productivity was determined to increase by more than 25 %. Our results indicate that statistical-based optimization of culture conditions effectively increases the cell mass and subsequently contributes to increased lutein production.
중력 여과장치 이용 미세조류 수확을 위한 여과막의 효율성 비교
신동우(Dong-Woo Shin),조용희(Yonghee Cho),김기현(Ki-Hyun Kim),김한별(HanByeol Kim),박한울(Hanwool Park),김지훈(Z-Hun Kim),임상민(Sang-Min Lim),이철균(Choul-Gyun Lee) 한국해양바이오학회 2017 한국해양바이오학회지 Vol.9 No.1
Cost-effective microalgae harvesting methods are necessary for economical production of algal biodiesel. In this study, membranes with various pore sizes and materials were examined for their potentials in application to gravity-filtration of Tetraselmis sp. KCTC12432BP. For this test, 10 L of Tetraselmis sp. culture (2 g/L) was loaded on each membrane and filtration rates were measured. Among the tested materials, a woven cotton fabric showed the fastest water drain rate (0.73 L/hr) without serious cell leakage. Cell density of the concentrates after filtration was 6.8 g/L, indicating 3.4-fold concentration compared with the initial algal culture. The result suggests that the woven cotton fabric could serve as filtration membrane for harvesting Tetraselmis sp. among the tested ones.
초기 육계 사료내 토착미세조류(Parachlorella sp.) 첨가에 따른 성장 및 면역반응 변화
안수현(Su Hyun An),주상석(Sang Seok Joo),이효건(Hyo Gun Lee),김지훈(Z-Hun Kim),이창수(Chang Soo Lee),김명후(Myunghoo Kim),공창수(Changsu Kong) 한국가금학회 2020 韓國家禽學會誌 Vol.47 No.1
The present study determined the effect of dietary cultivated microalgae (Parachlorella sp.) on the growth and immune responses of pre-starter broilers. A total of 320 one-day-old birds (Ross 308) were allocated to 4 treatments with 8 blocks in a randomized complete block design. The four experimental diets consisted of a corn-soybean meal-based control diet, and three diets contained 0.5%, 1.0%, and 1.5% microalgae powder at the expense of cornstarch in the control diet. After feeding the experimental diets for 7 days, the body weight and feed intake of all birds were measured, and 8 birds were randomly selected from each treatment. Peripheral blood mononuclear cells (PBMCs) and serum were harvested for immune profile assessment, including cytokines and cell migration receptors. No differences in growth performance were observed among the treatments. The birds that were fed diets containing graded levels of microalga showed a linear increase in the mRNA expression of cytokine genes in PBMCs, including that of IL2, IL1β, and IL18 (P<0.05). With respect to the chemokine receptor genes in PBMCs, mRNA expression of CCR2, CCR9, and ITGA4 changed quadratically (P<0.05), but that of CCR7 increased linearly (P<0.01). Cytokine protein secretion in blood, including that of IL-1β and IL-6, increased linearly (P<0.01) with an increase in the microalgal content. Overall, the present results show that the indigenous microalgae powder used in this study could stimulate immunity with no detrimental effects on the growth performance of pre-starter broiler chickens.