Leptomycin B Increases Radiosensitization by Trichostain A in HeLa Cells

In Ah Kim(김인아),Jin Ho Kim(김진호),Jin Hee Shin(신진희),Il Han Kim(김일한),Jae Sung Kim(김재성),Hong Gyun Wu(우홍균),Eui Kyu Chie(지의규),Yong Ho Kim(김용호),Bo Kyung Kim(김보경),Semie Hong(홍세미),Sung Whan Ha(하성환),Chan Il Park 대한방사선종양학회 2005 Radiation Oncology Journal Vol.23 No.2

목 적: 히스톤탈아세틸화효소 억제제는 그 자체의 항암효과뿐만 아니라 방사선 감작제로서의 효과가 점차 분명해져가고 있다. 최근 Class I 특이적인 히스톤탈아세틸화효소 억제제의 개발로 계층 특이적인(class specific) 연구가 가능해짐에 따라, 본 연구에서는 서로 다른 히스톤탈아세틸화효소억제제의 방사선감작효과를 비교함과 동시에 p53 발현도의 차이가 히스톤탈아세틸화효소억제제의 방사선 감수성에 미치는 영향을 알아보고자 하였다. 대상 및 방법: 이를 위해 p53 발현도가 매우 낮은 HeLa 세포에 p53의 핵 외 수송을 억제하여 세포질 내 분해를 차단하는 Leptomycin B를 처리하여 p53의 발현도를 현저하게 높인 후, Trichostatin와 SK7041의 방사선 민감도를 비교 관찰하였다. 결 과: 세포생존곡선, SER 및 SF2를 비교 분석 시, p53의 발현이 높은 Leptomycin B 처리군에서 TrichostatinA가 Class I HDAC만을 억제하는 SK7041에 비해 유의하게 높은 방사선 감작효과를 나타내었다. 이는 p53이 Class I 특이적 억제제인 SK7041과 Class I과 II를 모두 억제하는 TSA의 방사선감작효과에 미치는 영향의 차이에 기전적으로 관여함을 시사한다. 결 론: Leptomycin B에 의해 유도된 p53의 발현증가는 Class I과 Class I과 II를 모두 억제하는 TSA의 방사선 감작효과를 증강시킨다. Purpose: Histone deacetylase inhibitors (HDIs) are emerging as potentially useful components of anticancer therapy and their radiosensitizing effects have become evident. Specific HDIs are now available that preferentially inhibit specific HDAC classes; TSA inhibits Class I and II HDACs, and SK7041 inhibits Class I HDACs. Materials and Methods: We tested the differential radiosensitization induced by two different classes of HDIs in HeLa cells. We next tested the hypothesis that p53 expression in cancer cells may influence the susceptibility to HDIs by using pharmacologic modification of the p53 status under an isogenic background. Results: It is interesting that p53 expression in the HeLa cells clearly increased the degree of radiosensitization by TSA compared to that of the class I specific inhibitor SK7041. This suggests that p53 may, in part, be responsible for the mechanistic role for the greater radiosensitization induced by Class I & II inhibitors compared to that of the class I specific inhibitors. Thus, these studies are useful in distinguishing between events mediated solely by the Class I HDACs versus those events involving the other classes of HDACs as well. Conclusion: The anticancer efficacy of targeting Class I and II HDACs, in conjunction with radiation therapy, may be further enhanced by the restoration of p53 expression.

아토피 피부염 모델에 대한 β-1,3/1,6-glucan과 Lactobacillus plantarum LM1004의 면역 조절 효과

김인성(In Sung Kim),김성학(Sung Hak Kim),김정아(Jeong A Kim),유다윤(Da Yoon Yu),김광일(Gwang Il Kim),박동찬(Dong-Chan Park),임종민(Jong Min Lim),이상석(Sang Suk Lee),최인순(In Soon Choi),조광근(Kwang Keun Cho) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

본 연구에서는 아토피 피부염 동물 모델에 대한 β-1,3/1,6-glucan과 L. plantarum LM1004의 면역조절 효과를 확인하고자 하였다. 가려움증의 횟수와 유출된 evans blue, 그리고 혈청 IgE와 histamine의 농도는 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹에서 아토피 피부염 유발그룹에 비해 유의적으로 감소하는 결과를 나타내었다. 아토피 피부염이 유발되면 전사 수준에서 Th2 및 Th17 세포의 전사인자 및 cytokine은 과발현되며, β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취하였을 때 이를 유의적으로 감소되었다. 또한 β-1,3/1,6-glucan과 L. plantarum LM1004는 Th1 및 Treg 세포의 전사인자(T-bet, GATA-3, RORγT, Foxp3) 및 cytokine (INF-γ, IL-4, IL-17, TGF-β)의 발현을 증가시킴으로써 면역 균형을 조절하는 것으로 나타났다. Galectin-9과 filaggrin은 아토피 피부염 유발 처리군에서 유의적으로 가장 낮았으며, β-1,3/1,6-glucan 처리군에서 유의적으로 가장 높게 나타났다. 이와 반대로 TSLP는 아토피 피부염 유발그룹에서 유의적으로 가장 높았으며 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹은 대조군과 유사한 수준이었다. 이러한 결과를 통해 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염 동물 모델에서 면역조절 작용 및 아토피 피부염의 개선 효과를 가짐을 알 수 있었다. 따라서 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염에 유용한 천연소재로서 사용될 것으로 기대된다. In this study, we examined the efficacy of the immune regulation of β-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of β-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis–induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor γ T [RORγT]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of β-1,3/1,6-glucan and L. plantarum LM1004. In addition, β-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-γ [IFN-γ], transforming growth factor-β [TGF-β]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis–induced group and significantly higher in the β-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis–induced group, while mice that were orally administered β-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that β-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that β-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.

프로무용수의 열정과 자기인식 분석

김인선 ( In Sun Kim ),김승일 ( Seoung Il Kim ) 大韓舞踊學會 2009 대한무용학회논문집 Vol.60 No.-

This study aims to investigate and analyze passion and self-awareness of professional dancers from a diversified and in-depth perspective. To obtain this goal, this study conducted a qualitative case research on two currently active professional dancers. Relevant data was collected through in-depth interviews, documentation and descriptive survey research. The collected data was analyzed using encoding and the constant comparative method in order to verify the reliability, validity and ethical aspect of the study. The investigation of the passion and self-awareness of professional dancers is divided into dancers` personal dispositions, dancing activity and perception of the dancing community. Conclusions, based on the research methods and process of this study, are proposed as follows. First, the research participants exhibited differences in practice methods depending on personal dispositions. Since they are continuously active as professional dancers, most dancers use their leisure time for reinvigoration through relaxation, the modes of which also varied with personal propensities. Moreover, in terms of religious life, both survey participants showed commitment to and reliance on their respective beliefs. Dancer A claims to find psychological reassurance in prayers before stepping on stage, while dancer B believes he overcame an injury before an important concours through religion and faith. The personal dispositions of professional dancers differed drastically according to their sex. Second, regarding dancing activity, this research category was further divided into passion for social activity and dancing itself. In terms of social activity, dancer A adopts techniques that seem complimentary by observing the characteristics of fellow dancers. Regarding their wage, they believe their should be a gradation in accordance with dancers` performance, and were generally dissatisfied with their wage level. As for their passion for dancing, dancer A, her constant image training and movement practice attest to her sense of purpose and strong will to achieve set goals. Meanwhile, dancer B went as far as quitting highschool in order to learn dancing. he focuses as much on mental training through image training as he does on actual training. The participants showed passion to continue their career as a dancer either as a trainer, professor or a choreographer. Third, regarding their perception of the dancing community, the survey participants were aware of the importance of traditional dancing among other forms of creative activity. Furthermore, they were in agreement in their disappointment in the low valuation of culture and art in society.

Mechanism Underlying a Proteasome Inhibitor, Lactacystin-Induced Apoptosis on SCC25 Human Tongue Squamous Cell Carcinoma Cells

백철중,김규천,김인령,이승은,곽현호,박봉수,태일호,고명연,안용우,Baek, Chul-Jung,Kim, Gyoo-Cheon,Kim, In-Ryoung,Lee, Seung-Eun,Kwak, Hyun-Ho,Park, Bong-Soo,Tae, Il-Ho,Ko, Myung-Yun,Ahn, Yong-Woo Korean Academy of Orofacial Pain and Oral Medicine 2009 Journal of Oral Medicine and Pain Vol.34 No.3

Lactacystin, a microbial natural product synthesized by Streptomyces, has been commonly used as a selective proteasome inhibitor in many studies. Proteasome inhibitors is known to be preventing the proliferation of cancer cells in vivo as well as in vitro. Furthermore, proteasome inhibitors, as single or combined with other anticancer agents, are suggested as a new class of potential anticancer agents. This study was undertaken to examine in vitro effects of cytotoxicity and growth inhibition, and the molecular mechanism underlying induction of apoptosis in SCC25 human tongue sqaumous cell carcinoma cell line treated with lactacystin. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingiva fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay respectively. The hoechst staining, hemacolor staining and TUNEL staining were conducted to observe SCC25 cells undergoing apoptosis. SCC25 cells were treated with lactacystin, and Western blotting, immunocytochemistry, confocal microscopy, FAScan flow cytometry, MMP activity, and proteasome activity were performed. Lactacystin treatment of SCC25 cells resulted in a time- and does-dependent decrease of cell viability and a does-dependent inhibition of cell growth, and induced apoptotic cell death. Interestingly, lactacytin remarkably revealed cytotoxicity in SCC25 cells but not normal cells. And tested SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA contents, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, the up-regulation of Bax, and the activation of caspase-7, caspase-3, PARP, lamin A/C and DFF45 (ICAD). Flow cytometric analysis revealed that lactacystin resulted in G1 arrest in cell cycle progression which was associated with up-regulation in the protein expression of CDK inhibitors, $p21^{WAF1/CIP1}$ and $p27^{KIP1}$. We presented data indicating that lactacystin induces G1 cell cycle arrest and apoptois via proteasome, mitochondria and caspase pathway in SCC25 cells. Therefore our data provide the possibility that lactacystin could be as a novel therapeutic strategy for human tongue squamous cell carcinoma.

소아의 신혈관성 고혈압

강병철,하일수,김인원,정해일,최용,고광욱,Kang Byoung-Chul,Ha Il-Soo,Kim In-One,Cheong Hae-Il,Choi Yong,Ko Kwang-Wook 대한소아신장학회 1997 Childhood kidney diseases Vol.1 No.2

목적 : 소아 신혈관성 고혈압의 임상적 특징과 원인질환, 검사 소견 및 치료에 대한 반응 등을 분석하고자 본 연구를 시행하였다. 방법 : 1986년 1월부터 1994년 6월까지 서울대학교 어린이병원 소아과에 입원한 고혈압 환자 중 혈관조영술상 신혈관성 고혈압으로 진단된 16례에 대하여 병록지 검토를 통한 후향적 고찰을 시행하였다. 결과 : 전체 16례중 남녀비는 7:9이었고, 평균 연령은 8년 6개월이었다. 원인질환으로는 Takayasu 동맥염이 6례, Moyamoya병에 동반된 예가 5례, 섬유근성 이형증이 3례, 신경섬유종증에 동반된 예가 1례였으며, 1례에서는 원인 질환을 밝혀낼 수 없었다. 흔한 증상으로는 호홉곤란, 편측마비, 두통, 오심 등이 있었고, 신체검사 소견상 심비대, 간비대, 복부 청진상 잡음 등이 관찰되었다. 말초혈장 renin 활성도는 검사를 시행한 14례에서 모두 상승되어 있었다. 혈관조영술상 16례 중 9례가 양측성, 7례가 일측성 병변을 보였다. Captopril 신스캔은 시행된 전례에서 혈관조영술상의 병변과 일치되는 결과를 보였다. 약물치료만을 시행한 5례 중 2례에서는 혈압의 완전 정상화, 3례에서는 부분적 조절의 반응을 보였다. 경피적 혈관성형술은 10례에서 시도되었으나 5례에서는 실패하였고 성공한 5례 중 3례에서 재협착 또는 동반된 대동맥 협착등으로 다른 형태의 치료가 필요하였다. 수술적 치료는 4례에서 시행되었으며 모두 다른 형태의 치료로 실패한 경우였다. 이 중 3례에서는 혈압 조절에 실패하였고 1례에서는 부분적 조절의 반응을 보였다. 결론 : 소아의 신혈관성 고혈압의 원인 질환은 Takayasu 동맥염뿐 아니라 Moyamoya병, 섬유근성 이형증등 다양하므로 이들간의 감별이 필요하다. 여러 진단적 검사중 단독으로 확진 가능한 검사는 없으며 환자마다 적합한 진단적 검사법을 개별적으로 적용해야 한다. 치료로서 약물요법, 혈관성형술, 수술적 치료 등이 시행되지만 완치율 및 성공률은 아직 만족스럽지 못하다. Purpose : The clinical characteristics of renovascular hypertension (RVHT) in children were analyzed. Methods : Medical records of 16 children diagnosed as RVHT on the basis of angiography during Jan. '86 to Jun. 94 in our hospital were reviewed retrospectively. Results : The mean age at the onset was 8.5 yrs and the sex ratio(M:F) was 7:9. The causes of RVHT were Takayasu arteritis in 6, Moyamoya disease in 5, and fibromuscular dysplasia in 3 patients. Abdominal bruit was noted in 6 patients (38%). Peripheral renin activity was raised in all tested patients. Bilateral renal arterial involvemnent was found in 9 patients (56%). Captopril renal scans showed good correlation with angiographic findings. Five patients were treated with antihypertensives only, and blood pressure was controlled completely in 2 and incompletely in 3. Percutaneous transluminal angioplasty was performed in 10 patients with 50% of success rate. However, hypertension was recurred due to restenosis or accompaning aortic stenosis in 3 patients. Surgical treatment was performed in 4 patients, and the blood pressure was controlled partially in 1 and poorly in the remaining 3. Conclusions : Takayasu arteritis, Moyamoya disease and fibromuscular dysplasia are the major causes of childhood RVHT in our country. The diagnosis of RVHT in children should be based on a set of tests individually selected for case by case. For the low curability of the current treatment modalities available, RVHT in children should not be regarded as 'curable' so far. We expect, however, that the outcome will be improved by more extensive application of the newly developed surgical technique.

Lipoxygenase 결핍된 Non-GM콩의 특성

이수정(Soo-Jung Lee),김인성(In-Sung Kim),이혜진(Hye-Jin Lee),정종일(Jong-Il Chung),성낙주(Nak-Ju Sung) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.10

Lipoxygenase(LOX)가 결핍된 non-GM콩인 개척#1(검정콩, LOX2,3-free), 개척#2(황색콩, LOX2,3-free) 및 진양콩(황색콩, LOX1,2,3-free)의 이화학적 특성과 두부 수율을 LOX가 존재하는 태광콩(황색콩, LOX1,2,3-present)과 비교하였다. 콩의 길이 및 백립중은 태광콩에 비해 개척#1이 유의적인 차이로 컸으며, 황색콩에서는 LOX가 결핍된 품종이 태광콩보다 크기 및 중량이 다소 작았다. 콩을 12시간 동안 수침한 후 콩의 중량 증가율은 217.07~226.55%로 개척#2가 유의적으로 높았다. 전체적인 색차(ΔE)는 개척#1이 유의적으로 높았으며, 3종의 황색콩은 시료 간에 비슷한 경향이었다. 조지방 함량은 태광콩이 17.30%로 유의적으로 높았으며, LOX가 결핍된 콩은 13.71~14.66%로 시료 간에 유의차가 없었다. 조단백질 함량은 개척#1(34.79%)이 타 시료(32.93~33.47%)에 비해 유의적으로 높았다. 구성아미노산 함량은 모든 시료에서 glutamic acid의 함량이 가장 높았으며, 총량은 개척#1이 32.42 g/100 g으로 가장 많았고 개척#2와 태광콩은 비슷한 함량이었다. 이소플라본 함량은 LOX가 결핍된 콩이 태광콩에 비해 더 많았으며, 특히 개척#1(1672.54 μg/g)에서 가장 높았다. 콩의 총 페놀 함량은 개척#1이 276.91 mg/100 g으로 가장 높았으며, 플라보노이드 함량은 개척#1(26.93 mg/100 g)이 타 시료에 비해 2.6~2.8배 정도 높았다. DPPH, ABTS 라디칼 소거활성 및 환원력은 개척#1이 타 시료에 비해 높았다. Non-GM콩으로 제조한 두부의 수율은 개척#2에서 가장 높았다. 황색콩인 개척#2는 이화학적 특성 및 항산화 활성 측면에서 태광콩과 유사하였고 두부 수율이 높아 두부 제조에 적합한 품종인 것으로 사료된다. The properties of non-GM soybeans with lipoxygenase (LOX) free genotypes, such as Gaechuck#1 (black, LOX2,3-free), Gaechuck#2 (yellow, LOX2,3-free) and Jinyangkong (yellow, LOX1,2,3-free), were compared to Taekwangkong (yellow, LOX1,2,3-present) as control. Length and weight of soybeans were significantly longer in Gaechuck#1, but LOX free genotypes of yellow beans was smaller than Taekwangkong, in terms the size and weight. After soaking for 12 hr, increased ratios of soybean weight were 217.07~226.55%, and they were significantly higher in Gaechuck#2. The total color difference (ΔE) to Taekwangkong was the highest in Gaechuck#1, but another yellow soybean were similar. Crude lipids content was significantly higher in Taekwangkong (17.30%), and these LOX free genotypes (13.71~14.66%) did not show significant difference. Crude protein contents were significantly higher in Gaechuck#1 (34.79%) compared to the other soybeans (32.93~33.47%). Contents of glutamic acid among the detected composition amino acid were highest, and its total contents were highest in Gaechuck#1 (32.42 g/100g), and the total content of Gaechuck#2 was similar to Taekwangkong. Contents of total isoflavone were higher in LOX free genotypes than Taekwangkong, especially significantly higher in Gaechuck#1 (1672.54 μg/g). Contents of total phenol were highest in Gaechuck#1 (276.91 mg/100 g), and flavonoids content of Gaechuck#1 (26.93 mg/100g) was 2.6~2.8 times higher than the other samples. DPPH, ABTS radical scavenging activities and reducing power were significantly higher in Gaechuck#1. The yield of soybean curd from tested samples was the highest in Gaechuck#2. Gaechuck#2 as LOX free genotypes was similar to Taekwangkong with regard to physicochemical properties and antioxidant activities. Therefore, we propose that Gaechuck#2 is a more suitable genotype for soybean curd products.

Lipoxygenase 결핍콩으로 제조한 된장의 품질 특성 및 항산화 활성

이수정(Soo-Jung Lee),김인성(In-Sung Kim),호문사(Wen-Si Hu),하은선(Eun-Seon Ha),정종일(Jong-Il Chung),성낙주(Nak-Ju Sung) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.1

LOX 결핍콩(진양, 05C4 및 LS)의 가공적성을 평가하기 위하여 재래 된장을 제조하여 일반 콩(태광) 된장과 품질 특성 및 항산화 활성을 비교하였다. 된장에서 수분과 조단백질 함량은 LS 된장이 유의적으로 높았으며, 조지방 함량은 7.92~8.22%로 콩 품종에 따른 유의차가 없었다. 탄수화물함량은 LS 된장이 가장 낮았다. 된장의 환원당은 태광 된장에 비해 진양 된장이 유의적으로 높았다. 아미노태 질소 함량은 태광 된장에 비해 3종의 LOX 결핍콩 된장에서 유의적으로 높았다. 된장의 색깔은 태광 된장에 비해 05C4 및 LS된장은 차이가 없었으며, 진양 된장은 두드러진 색차를 보였다. 된장의 구수한 맛은 태광 된장이 다소 높았으며, 짠맛은 LOX 결핍콩 된장이 다소 높게 평가되었다. 향미는 LS 된장이 가장 강한 것으로 인지되었다. 전반적인 기호도는 시료간에 차이가 미미하였다. 총 아미노산 함량은 태광 된장에 비해 진양 및 05C4 된장에서 다소 높았으며, 필수 아미노산은 태광 된장에 비해 3종의 LOX 결핍콩 된장이 높게 정량되었다. 이소플라본의 총량은 태광 된장(374.79 μg/g)에 비해 3종의 LOX 결핍콩 된장(522.16~684.46 μg/g)에서 유의적으로 높았다. 총 페놀 함량은 태광 된장에 비해 05C4 및 LS 된장이 유의적으로 높았으며, 플라보노이드 함량은 LS 된장만 태광 된장에 비해 유의적으로 높았다. DPPH 라디칼소거 활성과 FRAP법에 의한 환원력은 진양 된장이 우수하였으나 ABTS 라디칼 소거 활성은 LOX 결핍콩 된장이 태광된장에 비해 유의적으로 높았다. LOX 결핍콩으로 제조한 된장은 아미노산과 이소플라본의 함량 및 항산화 활성 측면에서 된장 가공적성에 적합한 것으로 판단된다. To evaluate processing suitability of lipoxygenase (LOX)-free genotype soybeans (Jinyang, 05C4 and LS), quality characteristics and antioxidant activities of their Doenjang products were compared to those of Doenjang made from Taekwang soybean (LOX-present) as the control. Moisture and crude protein contents of Doenjang were significantly higher in LS than the control. Crude lipid content was not significantly different and was in the range of 7.92∼8.22% in all samples. Carbohydrate content was significantly lower in LS than Taekwang. Content of reducing sugar in Doenjang was significantly higher in Jinyang than Taekwang. Contents of amino-type nitrogen were significantly higher in LOX-free cultivars than Taekwang. Colors of 05C4 and LS Doenjang were not different compared to that of Taekwang, whereas Jinyang Doenjang showed a noticeable color difference. Taekwang Doenjang had a slightly stronger savory taste than another sample. LOX-free cultivars had a strong salty taste. Flavor was the strongest in LS, whereas overall acceptability showed little difference among all samples. The total amino acid content was slightly higher in Jinyang and 05C4 than Taekwang, and content of essential amino acids was higher in Doenjang from LOX-free cultivars than Taekwang. Isoflavon content was significantly higher in Doenjang (522.16∼684.46 μg/g) made from LOX-free cultivars than in Taekwang (374.79 μg/g). Total phenol content was significantly higher in 05C4 and LS, and flavonoid content was significantly higher in LS than Taekwang. Antioxidant activities were highest in Jinyang based on DPPH radical scavenging and reducing power. ABTS radical scavenging activity was significantly higher in Doenjang made from LOX-free cultivars than Taekwang. These results suggest that Doenjang from LOX-free cultivars could have a suitable genotype for Doenjang processing since it is more effective in terms of amino acids, isoflavone contents, and antioxidant activity.

히스톤 탈아세틸효소 억제제 SK-7041의 RIF-1 세포주에 대한 생체내 방사선 감수성 증진 효과

지의규(Eui Kyu Chie),신진희(Jin Hee Shin),김인아(In Ah Kim),김일한(Il Han Kim) 대한방사선종양학회 2010 Radiation Oncology Journal Vol.28 No.4

Purpose: To test the radiosensitizing effect of the newly synthesized novel histone deacetylase inhibitor, SK-7041 in vivo. Materials and Methods: The RIF-1 cell line was implanted into the back of a 6-week-old female C3H mouse, intradermally. The mice were grouped into control, drug, radiation (RT), and RT+drug group. SK-7041, 4 mg/kg was administered intraperitoneally for six cycles every 12 hours for mice in the drug and RT+drug group. An identical volume of phosphate buffered saline (PBS) was administered at the same frequency to mice in the control and RT groups. A single 5 Gy fraction was delivered to mice in RT and RT+drug group 6 hours after the fourth delivery. The volume of the implanted tumor was measured every 2∼3 days to formulate the growth delay curve. Results: For the control, drug, RT, and RT+drug groups, the average duration for implanted tumor to reach a volume of 1,500 mm3 was 10 days, 10 days, 9 days, and 12 days, respectively. Moreover, the tumor volume on D14 was 276.7 mm3, 279.9 mm3, 292.5 mm3, and 185.5 mm3, respectively (p=0.0004). The difference for the change in slope for the control and drug versus the RT and RT+drug groups were borderline significant (p=0.0650). Conclusion: The results of this study indicate that SK-7041 has a radiosensitizing effect for the RIF-I cell line in vivo at a low concentration and this effect may be synergistic. Implementing this result to clinical trial is warranted. 목 적: 국내에서 합성된 히스톤 탈아세틸 효소 억제제인 SK-7041의 생체내 방사선 감수성 증진 효과를 확인하고자 하였다. 대상 및 방법: 6주령의 웅형 C3H 마우스에 RIF-I 세포를 주입한 후 대조군, 약물군, 방사선군, 방사선 및 약물군으로 분류하였다. 약물군과 방사선 및 약물군에는 SK-7041 4 mg/kg을 12시간 간격으로, 6회에 걸쳐 복강 내 투여하였다. 대조군 및 방사선군에는 동일 용량의 phosphate buffered saline (PBS)를 같은 방법으로 투여하였다. 방사선군과 방사선 및 약물군에는 4번째 투약 후 6시간 후에 5 Gy를 1회 조사하였다. 이후 2∼3일 간격으로 종양의 크기를 측정하여 종양 성장 연기 곡선을 산출하였다. 결 과: 접종된 종양이 1,500 mm3까지 자라는 데 소요된 기간은 대조군, 약물군, 방사선군, 방사선 및 약물군에서 각각 10일, 9일, 9일, 12일이고, 14일째의 체적은 각각 276.7 mm3, 279.9 mm3, 292.5 mm3, 185.5 mm3로, 각 군간의 차이는 통계학적으로 유의하였다(p=0.0004). 아울러, 대조군과 약물군의 차이와 방사선군과 방사선 및 약물군의 차이를 비교하였을 때, 통계학적으로 유의한 경향을 보였다(p=0.0650). 결 론: 히스톤 탈아세틸 효소 억제제인 SK-7041의 RIF-I 세포주에 대한 마우스내 방사선 감수성 증진 효과를 저농도에서 확인할 수 있었으며, 방사선과 SK-7041의 상호작용은 상승 작용일 가능성을 시사하였다. 향후 환자에서의 적용 가능성에 대한 연구가 필요할 것으로 생각한다.

제약이론을 기반으로 한 최적제품조합 의사결정 지원 방법론 및 시스템

김인일(In Il Kim),한성환(Seong Hwan Han),권민철(Min Chull Kwon) 대한조선학회 2009 大韓造船學會 論文集 Vol.46 No.3

膽囊樣 巨大 膽石

金仁九(In-Koo Kim),朴炳日(Byung-Il Park),金鎭福(Jin-Pok Kim) 대한외과학회 1974 Annals of Surgical Treatment and Research Vol.16 No.12