THE EFFECT OF DIFFERENTIAL MODULATION OF N-METHYL-D-ASPART ATE RECEPTOR ON THE VIABILITY OF PRIMARY CULTURED NORMAL HUMAN ORAL KERATINOCYTES

김인수,이원,김성훈,최봄,Kim, In-Soo,Lee, Won,Kim, Seong-Hun,Choi, Bohm Korean Association of Maxillofacial Plastic and Re 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.4

In the present study, I investigated the effects of N-methyl-D-aspartate (NMDA), arachidonic acid (AA), and Nitric Oxide Synthase Inhibitor (NOS-I), alone or in combination, on the viability of cultured primary normal human oral keratinocytes (NHOK). Specifically, we examined whether AA and NOS-I could protect primary NHOK from glutamate cytotoxicity. The purpose of this study was therefore the preliminary study for the examination of the interaction between these agents and NHOK in order to elucidate the mechanisms by which epithelial growth and regeneration are regulated. NHOK were obtained from gingival tissue of 20 individuals aged 20 to 29, and third passage (P3) cells were used for this study. Cell viability was measured by the MTT assay. NMDA and NNA, a calcium dependent NOS inhibitor, induced an initial increase in cell number, which subsequently decreased by the $7^{th}$ day. Low concentration of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) induced an increase in cell number while high concentrations of AA ($5\;{\mu}M$ & $10\;{\mu}M$) induced a decrease in cell number. The decrease in cell number induced by NMDA at the $7^{th}$ day was abolished by the addition of low concentrations of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) or NOS inhibitors. Low concentrations of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) or NOS inhibitors may protect the NHOK from NMDA induced cytotoxicity. These reactions might be related to the NMDA receptor in the cell and the change of the intracellular calcium ion concentration.

황련해독탕 추출액의 각막염 원인균에 대한 항균효과

김인수,서진종,김광곤,하동룡,신민구,김의수,전상윤,Kim, In-Soo,Seo, Jin-Jong,Kim, Kwang-Gon,Ha, Dong-Ryong,Shin, Min-Koo,Kim, Eui-Su,Jeon, Sang-Yun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.3

Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of $70{\mu}l$ dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from $70{\mu}l$ to $10{\mu}l$ at the same concentration. Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract $70{\mu}l$ was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.

냉각가공한 STS 스테인리스강 주름관의 부식 특성에 관한 연구

김인수,김성진 ( In Soo Kim,Sung Jin Kim ) 한국부식방식학회 1993 Corrosion Science and Technology Vol.22 No.1

초고속 Myrinet 통신망에서의 PVM 성능 개선

김인수,심재홍,최경희,정기현,문경덕,김태근,Kim, In-Soo,Shim, Jae-Hong,Choi, Kyung-Hee,Jung, Gi-Hyun,Moon, Kyeong-Deok,Kim, Tae-Geun 한국정보처리학회 2000 정보처리논문지 Vol.7 No.1

본 논문에서는 초고속 통신망인 Myrinet을 기반으로 하는 워크스테이션 연동(network of workstations) 환경하에서 병렬 프로그래밍 모델을 지원하는 PVM (parallel virtual machine)의 통신 성능 개선 방안을 제시한다. PVM을 위해 새로이 제안하는 Myrinet 기반 통신 모델은 커널을 경유하는 기존의 UDP/IP 프로토콜을 이용하는 통신 모델과 Myrinet API를 직접 이용하는 통신 모델을 혼합한 복합 통신 모델이다. 제안된 복합 통신 모델은 사용자 영역(커널)에서 커널(사용자) 버퍼로의 메시지 복사 부하를 제거하고 커널내의 프로토콜 스택 처리를 위한 통신 지연 요인을 감소시키므로, Myrinet상에서 보다 빠른 PVM 테스크간의 데이터 전송 속도를 보장한다. 또한, UDP/IP를 사용하는 기존 PVM에 본 논문에서 제안된 Myrinet 기반 복합 통신 모델을 적용시킨 EPVM (Enhanced PVM)을 구현하고, 이의 성능을 측정하였다. 실험 결과 EPVM의 통신 성능이 기존 PVM 보다 평균 1.5배 정보 우수하다는 것을 확인하였다. PVM (parallel virtual machine) provides a programming environment that allows a collection of networked workstations to appear as a single parallel computational resource. The performance of parallel applications in this environment depends on the performance of data transfers between tasks. In this paper, we present a new Myrinet-based communication model of PVM that improves PVM communication performance over a high-speed Myrinet LAN. The proposed PVM communication model adopts a communication mechanism that allows any user-level process to directly access the network interface board without going through UDP/IP protocol stacks in the kernel. This mechanism provides faster data transfers between PVM tasks over the Myrinet since it avoids data copy overhead from kernel (user space) to user space (kernel) and reduces communication latency due to network protocol software layers. We implemented EPVM (Enhanced PVM), our updated version of the traditional PVM using UDP/IP, that is based on the proposed communication model over the Myrinet. Performance results show EPVM achieves communication speed-up of one to two over the traditional PVM.

경상분지(慶尙盆地) 언양단층(彦陽斷層) 지역(地域)에 대(對)한 전기비저항(電氣比抵抗) 탐사연구(探査硏究)

김인수,김종열,Kim, In-Soo,Kim, Jong-Yeol 대한자원환경지질학회 1983 자원환경지질 Vol.16 No.1

Gyeongsang Basin in the southeastern part of the Korean peninsular is characterized by many fault systems. To decipher the geotectonical evolution of the Korean peninsular and marginal basins in her adjacent areas it is prerequisite to understand the spatial distribution pattern and mutual relationships of these fault systems. Because of difficulties in finding any criterion to recognize the faults in field, their extension and mutual relationships in ages are not very clear yet. As an attempt to find geophysical criteria to recognize the fault, geoelectrical resistivity survey was carried out in this study. With the Wenner configuration four resistivity soundings and twenty seven resistivity profilings were done. The electrode distance used was up to 50m. From the results of the resistivity soundings and boring data of earlier groundwater investigations the depth of alluvial and weathered zone was established to be at most 20m in the study area. In the resistivity profiling low resistivity anomaly zones are detected on every traverse, which are interpreted as caused by fractures, fault clays and mylonites in the fault zone. The width of the fault zone amounts to 0.3-1km. By correlating and connecting the negative anomaly zones from traverse to traverse one can determine the trend of th of the faultzone and therefore that of fault itself. The recognized fault trend in this way was $N15^{\circ}-20^{\circ}E$ and this coincides with the direction of the inferred fault line from earlier geological surface mapping. With the help of this characteristical negative anomaly the existance of another $N80^{\circ}W$ trending fault was estabished. This study has shown that geoelectrical resistivity survey can be applied successfully to the problem of tracing fault line insofar as a fault zone has been developed along fault line.

경주-울산일원에 대한 지역지질 특성연구 : 울산단층주변 화강암류의 잔류자기와 대자율

김인수,손문,정현정,이준동,김정진,백인성,Kim, In-Soo,Son, Moon,Jung, Hyun-Jung,Lee, Joon-Dong,Kim, Jeong-Jin,Paik, In Sung 대한자원환경지질학회 1998 자원환경지질 Vol.31 No.1

A total of 469 granitic samples were collected from 44 sites in the Ulsan fault area, southeast Korea. According to the previous petrographic studies, the granitic rocks have been divided into four groups (Hornblende biotite granodiorite, Hornblende granite, Biotite granite and Alkali-feldspar granite). NRM intensities, values of low field magnetic susceptibility, and magnetic behaviors during stepwise demagnetization experiments suggest rather a three-fold classification: In this scheme, Hornblende granite and Biotite granite are grouped together, as they did not show any significant differences in magnetic characteristics. Based on the Ishihara (1979)'s criterion, Alkali-feldspar granite is classified as ilmenite-series granite, whereas others are classified as magnetite-series granite. In the eastern part of the study area including the Tertiary basin area, declinations of site-mean characteristic remanent magnetizations (ChRMs) show clockwise deflection of more than 30 from the reference direction of east Asia. Both along and in the adjacent region of the Ulsan fault-line, however, no deflection of remanent direction was observed. A boundary line between the deflected and undeflected site-mean ChRMs is defined in this study, which runs roughly parallel to the Ulsan fault-line at the distance of about 6km eastward from the fault-line. We suggest that this newly found boundary line, which we call Yonil tectonic line, released dextral simple shear stress acted in the southeastern part of the Korean peninsula during the opening stage of the East Sea in the Early Cenozoic.

BFTMA를 위한 측정데이터 전처리 기법 연구

김인수,Kim In-Soo 한국군사과학기술학회 2004 한국군사과학기술학회지 Vol.7 No.2

In this paper, the measurements preprocessing algorithm for the fading of bearing and frequency measurements is proposed, which can improve the performance of BFTMA(Bearing and Frequency Target Motion Analysis). The fading and detection relation between bearing and frequency are rigorously established for measurements preprocessing, and BFTMA can be carried out the estimation of target motion by using measurements preprocessing. Batch estimation with bearing and frequency using the proposed algorithm can be applied to estimate the initial target states despite of the fading of frequency measurement. Simulation results show that BFTMA using the proposed measurements preprocessing has superior estimation performance, compared with batch estimation using only bearing measurements.

고자기학(古磁氣學)의 방법(方法) (I)

김인수,Kim, In-Soo 대한자원환경지질학회 1984 자원환경지질 Vol.17 No.2

For the discussion of palaeogeomagnetic field direction and the movement and rotation of geotectonic blocks it is prerequisite to eliminate the secondary remanent magnetization component out of the NRM vector of given rock samples. Among various techniques alternating field and thermal demagnetization methods are most easily applicable and most widely used ones. Physical principle underlying these methods is the concept of relaxation time: Either with an action of external magnetic field or with an elevated temperature relaxation time of magnetic minerals can be drastically shortened. It has been furthermore shown experimentally that the secondary remanent magnetization can be more easily demagnetized than the primary remanent magnetization. Through careful stepwise demagnetization it should also be possible to discriminate the kind of various remanent magnetizations. In addition to the introduction to the underlying physical principles and experimental results of the alternating field and thermal demagnetization this paper gives various practical tips in earring out the demagnetization experiments. Each alternating field and thermal demagnetization instrumentation which is easily adaptable to domestic palaeomagnetic laboratories is also introduced. This paper provides a compact and practical introduction to the methods in palaeomagnetism and would be of interest to present workers and to those who want to embark on research in this field.

Pr 형과 Pfr 형 Phytochromd 의 단백질 표면의 소수성부위

김인수,송필순 ( In Soo Kim,Pill Soon Song ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.2

The nature of hydrophobic sites on the protein surfaces of the Pr and Pfr forms of phytochrome (118 kDa) has been studied by spectroscopic and electrophoretic properties of phytochrome that was partially digested with α -chymotrypsin. The enzyme digestion bleached the Pfr spectrum completely at a 1:60 (w/v) enzyme to substrate ratio, while the Pr absorbance was decreased by 20%. SDS-gel electrophoresis of the α -chymotrypsin-digested phytochrome showed that, at an early stage of digestion, the rate of hydrolysis was faster in the Pr form and the electrophoretic pattern of polypeptide digests was more complicated in the Pfr form than in the Pr form. The chromopeptide domain was more resistant to α -chymotrypsin digestion than the nonchromophore domain. The pronounced susceptibility of the Pfr spectrum to α -chymotrypsin was considered to be the action of the enzyme on hydrophobic site(s) at the chromophore vicinity that was newly developed by phototransformation from the Pr form to the Pfr form.

고자기학(古磁氣學)연구를 위한 자성광물론(磁性鑛物論)

김인수,Kim, In-Soo 대한자원환경지질학회 1983 자원환경지질 Vol.16 No.3

palaeomagnetism is a major implement to define tectonic provinces and to estimate their past relative position quantitatively. In this sort of investigation it is tacitly assumed that the magnetization of the rocks under study was acquired at the time of their formation. However, because of the possibility of secondary alteration and/or replacement of magnetic minerals, this assumption is not always legitimate. To secure reliable palaeomagnetic data it is therefore fundamental to identify the carrier mineral of magnetization. This paper reviews magnetic mineralogy relevant to palaeomagnetism of terrestrial rocks. Under the heading of each mineral its genesis, crystal structure, magnetic properties, criteria for ore microscopic determination and secondary alteration are summerized. This paper should also be helpful in application of magnetic mineralogy to geothermometry and oxygen barometry in igneous petrology, diagenesis and provenance study in sedimentary petrology, metamorphic temperature determination and genesis study of ore deposits.