황련해독탕 추출액의 각막염 원인균에 대한 항균효과

김인수,서진종,김광곤,하동룡,신민구,김의수,전상윤,Kim, In-Soo,Seo, Jin-Jong,Kim, Kwang-Gon,Ha, Dong-Ryong,Shin, Min-Koo,Kim, Eui-Su,Jeon, Sang-Yun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.3

Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of $70{\mu}l$ dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from $70{\mu}l$ to $10{\mu}l$ at the same concentration. Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract $70{\mu}l$ was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.

Smoothe Handoff 지원을 위한 빠른 인증 알고리즘

김인수,김기천,김현곤,Kim, In-Su,Kim, Gi-Cheon,Kim, Hyeon-Gon 한국정보처리학회 2002 정보처리학회논문지 C : 정보통신,정보보안 Vol.9 No.1

IMT-2000망 핵심기술은 mobile IP를 이용하는 북미의 3G packet data system과 GSM망과 연계를 하는 유럽의 GPRS로 구분할 수 있다. 이러한 IMT-2000망 핵심기술은 글로벌한 로밍을 위해 Mobile IP도입을 추진하고 있다. Mobile IP상에서는 RADIUS나 DIAMETER같은 AAA 서버가 다이얼 업 컴퓨터의 인증, 허가 서비스를 제공하기 위해 사용되고 있는데, 이것은 MN에게 매우 중요하다. Mobile IP는 MN과 HA간에 강력한 인증을 요구하기 때문이다. 본 논문에서는 이러한 IMT-2000 환경에서 고려되고 있는 Smooth Handoff 기법에서 효과적인 AAA프로토콜의 적용에 관하여 논한다. IMT-2000 technologies are divided 3G packet data system that using mobile IP and GPRS that based on the GSM networks. These technologies Push introduce mobile IP to support seamless roaming. In mobile If environments, use AAA server such as RADIUS or DIAMETER to provide authentication service for dial-up computers. This factor is important for mobile nodes. Mobile If require strong authentication between mobile nodes and home agents. We propose application of AAA protocols for smooth handoff mechanism in IMT-2000 environments.

양계 농업인의 작업장 환경 및 개인보호구 착용 실태조사

김인수,김경란,이경숙,채혜선,김성우,Kim, Insoo,Kim, Kyung-Ran,Lee, Kyung-Suk,Chae, Hye-Seon,Kim, Sungwoo 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.6

Objectives: This study was conducted to investigate the actual condition of the farm work environment and personal protective equipment as part of the effort to improve livestock work for the safety and health of poultry farmers and provide basic data for establishing plans to improve and develop personal protective equipment. Methods: For this purpose, a questionnaire survey on general information about stables, the poultry work environment, accidents, the wearing of work clothes and personal protective equipment, and the level of awareness related to personal protective equipment was conducted among 148 poultry farmers. Results: As a result, it was found that poultry workplace environment was exposed to such risks as fine dusts; organic dusts; poisonous gases; odorous substances; chicken excrement; contact with chickens, bacteria or viruses; and accidents related to machine operation. Thirteen percent of respondents suffered severe respiratory diseases, and the most frequently injured sites due to accidents were the hands (25.7%), knees (23.8%), arms (17.3%), and head (10.9%). The most frequent type of accident was collisions between the body and obstacles or machinery during movement (36.4%), followed by erroneous machine operation such as feeders and electric shocks (8.5%). Regarding the wearing of work clothes and personal protective equipment, 51.7% of the respondents wore worn-out clothing or everyday clothes, whereas only 32.0% wore work clothes. The percentage of farmers who wore proper protective equipment for the work environment during poultry work was 48.4%. The most frequently used type of protective equipment was boots (38.9%), followed by mask (36.7%), gloves (36.3%), appropriate work clothes (22.6%), quarantine clothes (17.6%), helmets (13.4%), and goggles (12.6%). The rate of wearing goggles was low because they were considered inconvenient and lowered work efficiency. Furthermore, they purchased everyday products available on the market for their personal protective equipment which were not appropriate for maintaining safety in an actual harmful environment and its consequent risks. As a result of the survey of the awareness level related to personal protective equipment, their levels of awareness of accidents and attitude proved to be average or higher, but the practice of wearing protective equipment and the level of knowledge and management of personal protective equipment were lower. Conclusion: This survey found that the wearing status of personal protective equipment among poultry farmers was insufficient even though they were exposed to risks. Most respondents were aware of the necessity of wearing personal protective equipment and of the potential for accidents, but they did not wear proper protective equipment. Their wearing rate was low due to a lack of knowledge about protective equipment, as well as the inconvenience of wearing it. Therefore there is a need to improve and develop specialized personal protective equipment for respiration, hands, and eyes, as well as work clothes that can protect farmers from major harmful matter that is generated in the poultry workplace. Based on the results of this investigation, we will conduct further studies on the required performance and design directions of personal protective equipment while collecting more objective data through field-oriented assessments.

동절기 일반국도 제설상황 관리.운영을 위한 도로제설관리시스템 소개

김인수,양충헌,전우훈,Kim, In-Su,Yang, Chung-Heon,Jeon, U-Hun 한국도로학회 2013 도로 : 도로학회지 Vol.15 No.3

LMI 에 기초한 연속 냉간압연기의 H^{\infty} 서보 제어기 설계

김인수,황이철,이만형,Kim, In-Soo,Hwang, I-Cheol,Lee, Man-Hyung 제어로봇시스템학회 2000 제어·로봇·시스템학회 논문지 Vol.6 No.1

In this paper, we design a H^\infty servo controller for gauge control of tandem cold mill. To improve the performance of the AGC(Aotomatic Gauge Control) system based on the Taylor linearized model of tandem cold mill, the H^\infty servo controller is designed to satisfy robust stability, disturbance attenuation and robust tracking properties. The H^\infty servo controller problem is modified as an usual H^\infty control problem, and the solvability condition of the H^\infty servo problem depends on the solvability of the modified H^\infty control problem. Since this modified problem does not satisfied standard assumptions for the H^\infty control problem, it is solved by an LMI(Linear Matrix Inequality) technique. Consequently, the comparison between the H^\infty servo controller and the existing PID/FF(FeedForward) controller shows the usefulness of this study.

Liposome 에 의한 식물 원형질체내로 Phytochrome 의 도입

김인수,임명호,송필순 ( In Soo Kim,Myung Ho Lim,Pill Soon Song ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2

Introduction of oat phytochrome into oat protoplast by liposomes has been studied as a tool to develop a system that could identify phytochrome receptor(s) in situ and an in vivo assay method of phytochrome activity. The encapsulation efficiency of phytochrome into liposomes was determined by fluorescence and radioisotope techniques; ^(125)I-labeled phytochrome was encapsulated into rhodamine-B-labeled liposomes and fluorescein-labeled phytochrome into ^(14)C-phosphatidylcholine (PC)-labeled liposomes. Phytochrome retained its characteristic absorbance spectrum, after encapsulated into liposomes by the Ca^(2+)-EDTA chelation method. Of three different compositions of liposomal lipids tested, liposome made of phosphatidylserine (PS) supplemented with 50% cholesterol (PS/Chol liposome) showed the highest encapsulation of phytochrome per unit amount of phospholipid (5.6×10^(13) molecules/μ㏖ PL). Phytochrome was also less associated on the surface of the PS/Chol liposome than on the surface of the other liposomes. When liposomes entrapping phytochrome were incubated with protoplasts, the amount of PL associated with protoplasts was higher with a hybrid liposome (PS/PC/Chol/stearylamine) but total number of phytochrome incorporated into protoplasts was higher with the PS/Chol liposome, apparently due to the leakage of phytochrome during liposome-protoplast interactions. The PS/Chol liposome prepared by the Ca^(2+)-EDTA chelation method found suitable for delivering phytochrome into protoplasts. Under the conditions tested in the present study, 2∼3×10^6 phytochrome molecules could be introduced into a protoplast.

黃連解毒湯, 黃連, 黃芩, 黃柏, 梔子의 Salmonella Typhimurium에 대한 항균효과

김인수,이영수,Kim, In-soo,Lee, Young-su 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.2

Objectives: This experiment investigated the antimicrobial activity of Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , and Gardenia jasminoides against Salmonella typhimurium . Methods: After spreading S. typhimurium on a bacterial culture medium plate, antimicrobial activity was tested by dripping diluted Hwangryunheadok-tang or extracts of Scutellariae radix , Phellodendri cortex , Coptis rhizome , or Gardenia jasminoides (80 μl, diluted 100, 50, 10, and 1%) onto the plate, followed by culture for 16 to 72 hours. The minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution that showed antimicrobial activity (80, 60, 40, and 20 μl) and measuring the density. The antimicrobial activity of Hwangryunheadok-tang and four extracts showed continuous antibacterial activity against S. typhimurium throughout the experiment. Result: 1. S. typhimurium . (Standard Microorganism, ATCC) 1) The Hwangryunheadok-tang and extracts of Scutellariae radix , Phellodendri cortex , and Coptis rhizome showed antibacterial activity in the undiluted solutions and in 50% dilutions. Gardenia jasminoides extract showed potency only in the undiluted solution. The antimicrobial potency of the undiluted solution was increased when the volume of inoculation was increased, but no difference was noted when the culture time was extended. All the extracts showed antimicrobial potency against S. typhimurium . 2. S. typhimurium isolated from diarrhea patients 1) When compared to the standard microorganism experiment on S. typhimurium , the MICs of the five extracts were increased. However, whereas the antimicrobial potency of doxycycline was lost entirely against bacteria isolated from patients with diarrhea, the antimicrobial potency of all the extracts was diminished but did not disappear. 2) The antimicrobial activity of Hwangryunheadok-tang and four extracts was continuous even when the culture time was extended to 16, 24, and 72 hours. Conclusions: The Hwangryunheadok-tang and four kinds of extracts have antimicrobial activity against S. typhimurium . Comparison of a standard microorganism with S. typhimurium isolated from diarrhea patients showed that the antimicrobial activity of all the extracts was better than that of antibiotics. Further studies should focus on the value and benefits of Hwangryunheadok-tang , and the four kinds of extracts as clinical treatments.

V2V 기본 안전 메시지 데이터의 유효성 검증

김인수,박재홍,이은영,이은덕,신재곤,김대원,Kim, Insu,Park, Jaehong,Lee, Eun-Young,Lee, Eun Dok,Shin, Jaekon,Kim, Daewon 한국자동차안전학회 2017 자동차안전학회지 Vol.9 No.2

In recent days, crash safety system based on vehicle-to-vehicle communication (V2V) has been legislated. This V2V based safety system collects information from nearby vehicles to predict any crash possibility. Thus, it requires accurate and reliable data. Regularly updated features of Basic Safety Message(BSM) will be used to test validity of various elements included in the BSM. Then, the focus was made on whether values of these elements had notable differences compared to previous values. Through this paper, the validation tool was implemented and the result from V2V OBU experiment was used to identify problems in the current model and additional features that need to be implemented in V2V OBU for more accurate BSM.

藥水 수질 特性에 관한 연구 -전남지역을 중심으로-

김인수,하훈,서원술,배주순,문희,박철웅,오은하,이소영,김명혜,kim, I.S.,Ha, H.,Seo, W.S.,Bae, J. S.,Mun, H.,Park, C.U.,Oh, E.H.,Lee, S.Y.,Kim, M.H. 한국환경보건학회 1998 한국환경보건학회지 Vol.24 No.1

This study was performed to investigate the Natural mineral water quality at 47 sites in Chonnam area, samples were collected spring, summer, fall, winter for a seasonal variation in 1996. The results obtained were summarized as follows 1. The mean of F, pH, HCO$_3$, NO$_3$ -N were 0.19 mg/l, 6. 9, 34.99 mg/l, 1.40 mg/l and those of Na, K, Ca, Mg were 12.1 mg/l, 1.2 mg/l, 7.7 mg/l, 2.1 mg/l respectively. 2. The contents of F were reasonable at Multonggol as 1.35 mg/l and Yongbayui as 0.57 mg/l respectively, but there is no detected at Sidong and detected below 0.1 mg/l at Gubongsan, Hansansa area. 3. According to the geological effect, the content of F was showed higher in granite than in tuff, and index of pH was higher in tuff than in andesite, and content of HCO$_3$ was higher in andesite than in granite. 4. The K and O values were used as index of healthy and delicious water. As a result, thirtyfive sites(74.5%) was decided as healthy and delicious water. 5. The contents of NO$_3$-N were detected over 5 mg/l at Sukchonsa, Gajanggol, Yudalsan, Muchang, Kisan.

THE EFFECT OF DIFFERENTIAL MODULATION OF N-METHYL-D-ASPART ATE RECEPTOR ON THE VIABILITY OF PRIMARY CULTURED NORMAL HUMAN ORAL KERATINOCYTES

김인수,이원,김성훈,최봄,Kim, In-Soo,Lee, Won,Kim, Seong-Hun,Choi, Bohm Korean Association of Maxillofacial Plastic and Re 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.4

In the present study, I investigated the effects of N-methyl-D-aspartate (NMDA), arachidonic acid (AA), and Nitric Oxide Synthase Inhibitor (NOS-I), alone or in combination, on the viability of cultured primary normal human oral keratinocytes (NHOK). Specifically, we examined whether AA and NOS-I could protect primary NHOK from glutamate cytotoxicity. The purpose of this study was therefore the preliminary study for the examination of the interaction between these agents and NHOK in order to elucidate the mechanisms by which epithelial growth and regeneration are regulated. NHOK were obtained from gingival tissue of 20 individuals aged 20 to 29, and third passage (P3) cells were used for this study. Cell viability was measured by the MTT assay. NMDA and NNA, a calcium dependent NOS inhibitor, induced an initial increase in cell number, which subsequently decreased by the $7^{th}$ day. Low concentration of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) induced an increase in cell number while high concentrations of AA ($5\;{\mu}M$ & $10\;{\mu}M$) induced a decrease in cell number. The decrease in cell number induced by NMDA at the $7^{th}$ day was abolished by the addition of low concentrations of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) or NOS inhibitors. Low concentrations of AA ($0.5\;{\mu}M$ & $1\;{\mu}M$) or NOS inhibitors may protect the NHOK from NMDA induced cytotoxicity. These reactions might be related to the NMDA receptor in the cell and the change of the intracellular calcium ion concentration.