여주(Momordica charantia) 추출물이 생쥐의 지구력 운동수행능력 향상 효과에 미치는 영향

김인보,박춘호,정회윤,정주성,홍환웅,김종배,Kim, Inbo,Park, Choon-Ho,Jung, Hoe-Yune,Jeong, Juseong,Hong, Hwan-Ung,Kim, Jong Bae 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.4

Bitter melon (Momordica charantia) is used in traditional herbal medicine in many Asian countries for the treatment of several diseases such as diabetes, eczema, night blindness, psoriasis, and rheumatism. Especially, most reports concerning the biological activities of bitter melon have focused on its effects on diabetes and hyperglycemia. Also, bitter melon is regarded as a longevity food, suggesting that it has several beneficial effects on anti-aging and the maintenance of a healthy state. Thus, we investigated whether bitter melon could increase the capacity of exercise in this study. Interestingly, bitter melon fruit extract activated AMP-activated protein kinase (AMPK), which is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. In addition, bitter melon extract increased the expression of enzymes involved in fatty acid oxidation such as mitochondrial uncoupling protein 3 (UCP3), carnitine palmitoyl transferase 1b (CPT1b), and pyruvate dehydrogenase lipoamide kinase isozyme 4 (PDK4). Moreover, exercise tolerance was much more enhanced in bitter melon treated animals compared to the non-treated control group. These results suggest that bitter melon is a promising candidate for the development of functional foods beneficial for physical strength and the enhancement of exercise capacity.

한국산 겨우살이 숙주별 렉틴 함량과 지표물질로서의 특성 조사

김인보,윤택준,박춘호,이우경,이소희,김종배,Kim, Inbo,Yoon, Taek Joon,Park, Choon Ho,Lee, Woo Kyoung,Lee, So Hee,Kim, Jong Bae 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6

겨우살이는 전통적으로 항암활성이 있는 약용식물의 하나로 알려져 왔고, 렉틴은 세포독성 및 면역자극 자극 활성을 가지는 대표성분으로 인정되고 있다. 한국산 겨우살이에 함유되는 렉틴은 유럽산의 그것과는 달리 galactose와 N-acetylgalactosamine(GalNAc) 특이성을 동시에 가지는 렉틴 성분인 KML인 것으로 나타났다. Sandwich ELISA법을 이용하여 각기 다른 종류의 숙주나무에서 유래된 5종의 겨우살이로부터 렉틴 함량을 비교한 결과, 숙주나무별 차이가 인정되어 밤나무 겨우살이는 참나무 겨우살이에 비하여 약 10배 많은 렉틴을 함유하고 있었다. L5178Y-ML25 lymphoma 세포에 약 90%의 세포독성을 나타내는 농도의 KML과 한국산 참나무 유래 겨우살이 추출물인 KM-100에 두 종류의 단일클론 항체(9H7-10 and 8B11-2C5)를 동시처리한 후 세포독성 중화효과를 조사한 결과, KML의 경우 약 10%, KM-110의 경우 약 30%의 세포독성을 보였다. 이러한 결과는 겨우살이에서 렉틴 외에도 세포독성을 가지는 다른 성분이 존재할 것으로 사료되었다. RAW 264.7 대식 세포주에 KM-110과 KM-110으로부터 렉틴이 제거된 분획인 LFKM-110을 자극시킨 결과, LFKM-110에서 $TNF-{\alpha}$ 및 IL-6와 같은 cytokine의 생산을 증진시키는 결과를 보였다. 따라서 KM-110에서 면역 세포를 자극하는 다른 성분의 존재하고 있음을 강하게 제시되었다. Traditionally, mistletoe is known as an effective anti-cancer medicinal plant, and lectin is recognized as a major component with cytotoxic and immuno-stimulant activity in mistletoe. A Korean mistletoe lectin (KML) has specificity to galactose and galactosamine and is distinguish from European mistletoe lectin (EML). When we examined the concentration of lectin in mistletoe originated from five different types of host trees, the result indicate that the lectin concentration is variable depending on the host tree. Noticeably, mistletoe from chestnut tree contains ten folds higher lectins than that of an oak tree. We also tested the concentration of KML and crude extract (KM-110) of Korean mistletoe that shows 90% cytotoxicity in L5178Y-ML25 lymphoma cell. In addition, the cells show 90% and 70% viability by the treatment of two neutralizing antibodies of KML, 9H7-D10 and 8B11-2C5 neutralization effect with two monoclonal antibodies of KML, 9H7-D10 and 8B11-2C5. Therefore, the result expected that the mistletoe contain some other cytotoxic components except lectin. Finally, the production of $TNF-{\alpha}$ and IL-6 by RAW 264.7 cells stimulated with lectin free-crude extract (LFKM-110) following neutralization by 9H7-D10 monoclonal antibody shows higher than that of lectin containing-crude extract (KM-110). These results suggest that the Korean mistletoe lectin ha a great potential to be developed as therapeutic agent of cancer.

다양한 변환 공진기에 형성되는 비등방성 속삭임의 회랑 모드

김인보,최무한,Kim, Inbo,Choi, Muhan 한국광학회 2018 한국광학회지 Vol.29 No.3

이 논문에서는 변환 광학의 기본 원리를 설명하고 이를 적용하여 공진기 모양이 원형으로부터 크게 변형되어도 속삭임의 회랑 모드(WGM)의 특성을 유지할 수 있는 다양한 변환 공진기 설계 기법을 소개한다. 특정 등각 변환 하에서 다양한 변환 공진기를 얻는 방법으로서 초기 정의역을 평행 이동시키는 방법과, 타원을 정의역으로 잡고 회전시키는 방법을 제시하였고, 이를 통해 설계된 몇 가지의 공진기 모드에 대한 내부 파형 및 원거리장 출력 특성을 수치해석적으로 계산하였다. 이러한 결과로부터, 하나의 주어진 등각 좌표변환과 관련된 다양한 GRIN 공진기가 가능하다는 것을 확인하였다. We explain the basic principle of transformation optics, and introduce several design techniques for transformation cavities that can maintain the characteristics of whispering gallery modes (WGMs) even if the cavity is strongly deformed from a circular shape. As a method of obtaining various transformation cavities under a specific conformal mapping, we suggest a method of parallel movement of the initial cavity domain, and a method of rotating the ellipse as a cavity domain. The internal wave pattern and the far-field output characteristics of several designed resonant modes are numerically calculated. From these results, it is confirmed that a variety of GRIN resonators are possible for a given conformal coordinate transformation.

Erratum: Affiliations Correction

정회윤,유영춘,김인보,성낙윤,최옥병,최보화,김종배,Jung, Hoe-Yune,Yoo, Yung Choon,Kim, Inbo,Sung, Nak Yun,Choi, Ok-Byung,Choi, Bo-Hwa,Kim, Jong-Bae The Korean Society of Food Science and Nutrition 2015 한국식품영양과학회지 Vol.44 No.6

테라헤르츠 이미징을 위한 능동형 메타디바이스

최무한(Muhan Choi),김인보(Inbo Kim),유정완(Jung-Wan Ryu),한재형(Jae-Hyung Han) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

근위축 마우스 모델에서 한국산 겨우살이 추출물에 의한 단백질 합성 신호전달 경로의 활성화

정주성 ( Juseong Jeong ),박춘호 ( Choon-ho Park ),김인보 ( Inbo Kim ),김종배 ( Jong-bae Kim ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.2

Muscle atrophy is characterized by a decrease in the mass of the muscle. With an increase in life expectancy and chronic illnesses, the incidence of muscle atrophy is increasing and the quality of life of patients is decreasing. Thus, reducing muscle atrophy is of high clinical and socio-economic importance. Mistletoe is a semi-parasitic plant that has been used as a traditional medicine in many countries to treat various human illnesses. It has been reported that Korean mistletoe extract (KME) has diverse biological functions including anti-tumor, anti-oxidant, anti-diabetic, anti-obesity properties, and extension of lifespan. Especially, we have recently reported that KME improves exercise endurance in mice, indicating its beneficial roles in enhancing the capacity of skeletal muscle. In this study, we investigated whether KME could activate the signaling pathway related to protein synthesis in a mouse model of muscle atrophy. Interestingly, KME efficiently activated the Akt/mTOR pathway, and Akt and mTOR are important signaling hub molecules for the acceleration of protein synthesis in muscle cells. In addition, KME also increased the activity of S6 kinase which is involved in the regulation of muscle cell size. Moreover, the ERK activity, required for transcription of ribosomal RNA for protein synthesis, was also enhanced in KME-treated mouse muscle. These data support the idea that KME increases muscle mass via increased protein synthesis. Our findings also suggest that Korean mistletoe might be a promising candidate for the development of functional foods that are beneficial for preventing muscle atrophy.

한국산 겨우살이 추출물(KME)의 2형 당뇨 억제 및 근육세포 미토콘드리아 생성 증가 효과

정회윤(Hoe-Yune Jung),유영춘(Yung Choon Yoo),김인보(Inbo Kim),성낙윤(Nak Yun Sung),최옥병(Ok-Byung Choi),최보화(Bo-Hwa Choi),김종배(Jong-Bae Kim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.3

본 연구에서는 C57BL/6J ob/ob 마우스를 이용하여 한국산 겨우살이 냉수 추출물(KME)의 항당뇨 활성을 조사하였다. 50 혹은 100 mg/kg의 KME를 1일 1회씩 경구투여 한 결과 KME 투여 개시 5일 후부터 ob/ob 마우스의 혈당이 유의하게 억제되었으며, 10일 후부터 안정된 억제 효과를 나타내고 대조군에 비해 20% 이상의 혈당강하 효과를 나타내었다. 경구 당부하 실험(OGTT)에서는 KME 경구투여 마우스에서 유효한 당부하 억제 활성이 관찰되었다. 또한 KME 경구투여는 ob/ob 당뇨 마우스의 혈액 내 총 콜레스테롤과 중성지질의 농도를 억제하는 것으로 나타났다. 한편 C2C12 근육세포를 이용한 in vitro 실험에서 KME를 처리함으로써 glucose uptake가 현저히 증가하였다. 한편 매우 흥미롭게도 KME를 처리한 C2C12 근육세포에 있어서 미토콘드리아 생성과 산화대사 조절물질인 peroxisome proliferatoractivated receptor gamma coactivator 1-α(PGC-1α)를 비롯하여 glucose transporter type 4(GLUT4), estrogen-related receptor-α(ERR-α), nuclear respiratory factor-1(NRF-1) 그리고 mitochondrial transcription factor A(TmfA)와 같은 PGC-1α 관련 유전자들의 발현이 증가하는 것으로 확인되었다. 이 결과는 KME가 2형 당뇨에 대한 치료물질로서의 작용을 지니며 이러한 KME의 항당뇨 활성은 미토콘드리아 생성의 조절과 관련 있는 것으로 추정된다. In this study, the anti-diabetic activity of a cold water extract of Korean mistletoe (KME) was investigated in C57BL/6J Lep ob (ob/ob) mice. Oral administration of KME (50 or 100 mg/kg/d) significantly inhibited the level of blood glucose of ob/ob mice after 5 days from the beginning of KME treatment. And the anti-diabetic effect of KME was stabilized 10 days after oral administration, showing a substantial reduction of blood glucose levels by more than 20% as compared with control mice. The results of oral glucose tolerance test (OGTT) revealed that oral administration of KME gave rise to a remarkable improvement in overall glucose response. Oral administration of KME in ob/ob diabetic mice also significantly reduced blood total cholesterol (TCHO) and triglyceride (TG) levels compared with the diabetic control mice. Moreover, in an in vitro experiment using C2C12 myotubes, treatment of KME prominently increased glucose uptake. Interestingly, KME significantly increased the expression of peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α), a head regulator of mitochondrial biogenesis and oxidative metabolism, and PGC-1α-associated genes such as glucose transporter type 4 (GLUT4), estrogen-related receptor-α (ERR-α), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TmfA) in C2C12 cells. These results suggest that KME has potential as a novel therapeutic agent for diabetes, and its anti-diabetic activity may be related to the regulation of mitochondrial biogenesis.