생쥐 난포의 체외배양 중 생식샘자극호르몬에 따른 미세리보핵산 발현 양상

김용진,구승엽,김윤영,오선경,김석현,최영민,문신용,Kim, Yong-Jin,Ku, Seung-Yup,Kim, Yoon-Young,Oh, Sun-Kyung,Kim, Seok-Hyun,Choi, Young-Min,Moon, Shin-Yong 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.4

목 적: 미세리보핵산 (microRNA, miR)은 전사 후 (post-transcriptional) 단계에서 목표 유전자 (target gene)의 발현을 억제하여 세포의 발달과 성장에 중요한 역할을 하는 것으로 알려져 있다. 그러나 난포의 성장과정 중의 miR 발현 양상에 대해서는 잘 알려져 있지 않다. 따라서 존 연구는 생쥐 난포의 체외배양 후 생식샘자극호르몬 (gonadotropin)과 사람융모성생식샘자극호르몬 (hCG) 첨가에 따른 난자와 난구세포에서의 miR 발현 양상을 살펴보고자 수행되었다. 연구방법: 생후 12일된 생쥐 (C57BL6)의 난소 적출 후, 전동 난포 (preantral follicle)를 분리하여 무작위로 $20\;{\mu}L$ 점적의 배양액만 있는 군 (control group), 재조합 난포자극호르몬을 첨가한 군 (FSH group), 재조합 황체형성호르몬을 첨가한 군 (LH group), FSH와 LH를 같이 첨가한 군 (FSH+LH group)으로 나누어 배양하였다. 난포가 충분히 성장하였을 때, 다시 hCG를 첨가한 군 (hCG (+) group)과 첨가하지 않은 군 (hCG (-) group)으로 나누어 hCG (-) group에서 난자와 난구세포를 각각 분리하여 RNA를 추출하였다. 36시간 후, 배란된 난자 난구세포 복합체 (cumulus oocyte complex, COC)에서 난자와 난구세포를 각각 분리하여 RNA를 추출하고, mmu--miR-16, -miR-27a, -miR-126, -miR-721 등의 miR에 대한 primer를 이용하여 실시간 중합연쇄반응을 시행하였다. 결 과: 배란율과 MII 난자 생성율은 다른 군들에 비해 FSH+LH군에서 유의하게 높았다. 각 군내의 난자와 난구세포 사이에서도 miR 발현 양상의 차이가 관찰되었다. 또한, 난자와 난구세포에서의 miR 발현 양상은 각 군간 차이가 있었으며, hCG (+)군과 hCG (-)군간에도 차이를 나타냈다. 결 론: 생쥐 난포의 체외배양 중 난자 및 난구세포에서의 miR 발현 양상은 gonadotropin의 종류 및 난자의 성숙도에 따라 다르다. 이러한 결과는 표적 유전자의 발현에 대한 추후 연구를 통한 확인이 필요할 것으로 사료된다. Objective: MicroRNAs (miR) are known to repress target genes at post-transcriptional level and play important roles in development and maturation of cell. However, the expression profiles of miR during ovarian follicle maturation have not been fully elucidated. Here, we designed this study to investigate the expression profiles of miR in oocytes and granulose cells (G-cells) after in vitro culture according to gonadotropins and adding hCG. Methods: Ovaries from 12-day-old mice (C57BL6) were removed and preantral follicles were isolated and cultured in $20\;{\mu}L$-drop of culture media with supplementation of either rFSH, rLH, or rFSH+rLH. After their full maturation, follicles were incubated with rhCG and rEGF. RNA was isolated from oocytes and G-cells, and real-time PCR were performed with primers of miR known to be expressed in the mouse ovary (mmu-miR-16, -miR-27a, -miR-126, -miR-721). Results: FSH+LH group showed the highest ovulation and MII rates among gonadotropin groups. The profiles of miRs in oocytes and G-cells differed according to gonadotropin groups and adding hCG. The profiles of miRs showed divergent changes between oocytes and G-cells. Conclusion: miR expression profiles are altered by gonadotropins and supplementation of hCG during in vitro maturation of murine follicles. Target gene study must be necessary to validate these findings.

자가 정맥 이식을 이용한 수지 접합술

김용진,김형건,김형주,박진철,Kim, Yong-Jin,Kim, Hyung-Kun,Kim, Hyung-Ju,Park, Jin-Chul 대한미세수술학회 1998 Archives of reconstructive microsurgery Vol.7 No.1

We carried out 107 replantations of amputated digits since last 1988. Among them, clean Gillotine amputation was 19 digits, mild crush amputation was 51 digits, severe crush amputation was 28 digits and avulsion type amputation was 9 digits. Vein grafts were used on 24 of these cases. The donor site of the vein graft was the volar aspect of the ipsilateral wrist or lower forearm. The average length of the vein graft was 1.8cm(1.0-3.0cm) and the average diameter of the vein graft was 1.5mm(1.0-2.0mm). Sixty-one of 107 digits were successfully replanted for an 57% success rate. The survival rate of the replantation using the vein graft was 66.7%. We analysed the functional results of the successfully replanted digits according to Nakamura and Tamai's criteria. The functional results of the replantated digits using vein graft were better than that of without using vein graft(more than fair results, 81.2% vs 73.3%). We conclude that the jucidious use of autogenous vein grafts in digital replantation surgery will improve the survival rate and promote the functional result.

가스분무 공정에 의한 고강도 과공정 Al-Si 합금 분말의 제조 및 특성 연구 I. 분말 제조 및 성형성

김용진,김진천,Kim Yong-Jin,Kim Jin-Chun 한국분말야금학회 2005 한국분말재료학회지 (KPMI) Vol.12 No.4

In order to improve mechanical properties, the hypereutectic Al-20 wt%Si based prealloy powder was prepared by gas atomization process. Microstructure and compressibility of the atomized Al-Si powder were investigated. The average powder size was decreased with increasing the atomization gas pressure. Size of primary Si particles of the as-atomized powder was about $5-8\;\mu{m}$. The as-atomized Al-Si powder such as AMB 2712 and AMB 7775 to increase compressibility and sinterability. Relative density of the mixed powder samples sintered at $600^{\circ}C$ was reached about 96% of a theoretical density.

장막침윤이 없는 위암환자에서 수술 후 보조적 화학 요법에 대한 전향적 연구 -최종보고-

김용진,김병식,김용호,육정환,오성태,박건춘,Kim Yong Jin,Kim Byung Sik,Kim Yong Ho,Yook Jung Hwan,Oh Sung Tae,Park Kun Choon 대한위암학회 2004 대한위암학회지 Vol.4 No.4

목적: 장막침윤이 없는 위암환자에 있어서 수술 후 서로 다른 보조화학요법이 장기 생존에 미치는 영향에 대해 평가하고자 하였다. 대상 및 방법: 1996년 10월부터 1998년 3월까지 만 15개월간 서울 아산병원 외과에서 근치적 위 절제를 시행한 환자 중, 장막침윤이 없는 317명의 환자를 대상으로 하였다. 이중 수술 후 병기가 IA인 131예, 장막침윤이 있는 34예, 그리고 치료를 거부한 17예를 제외한 135명(1군: 5-FU+cisplatin 정주, 2군: mitomycin C정주+경구용5-FU, 3군: 경구용 5-FU)의 의무기록 분석과 전화추적을 실시하여 재발양상, 생존율, 및 위암관련 사망률을 각 군별로 분석하였다. 결과: 121명에서 추적이 가능해 $89.6\%$의 생존율을 나타냈다. 재발은 1군 4명, 2군 7명, 그리고 3군 6명 이었으며, 세 군의 전체생존율은 1군 $89\%$, 2군 $84\%$, 그리고 3군은 $82\%$이고, 위암관련 사망환자만을 고려한 질병특이 생존율은 1군 $92\%$, 2군 $86\%$, 그리고 3군은 $88\%$로 나타나 이들 세 군간에 전체생존율 및 질병특이 생존율은 차이를 보이지 않았다(전체생존율: P=0.6875, 질병특이 생존율: P=0.7120). 결론: 장막침윤이 없는 위암 환자에서, 치료효과를 향상 시키기 위해 보조요법을 시행할 경우 경구용 항암제를 이용한 방법이면 충분하다고 판단하였다. 그러나 이를 입증하기 위해서는 보조요법을 시행치 않는 대조군을 포함한 대단위 무작위 연구가 필수적일 것이다. Purpose: We reported our preliminary result in 2001. At that time, the follow-up period was too short to evaluate the survival benefit of adjuvant chemotherapy in gastric cancer without serosal invasion. Therefore, we followed those patients for 66 months to determine the long-term effects of adjuvant chemotherapy. Materials and Methods: We analyzed the recurrence pattern, the survival rate, and the disease-specific survival of 135 patients by reviewing their medical records and calling the patients or their relatives. All enrolled patients were included in the intention-to-treat analysis of efficacy. Results: The follow-up rate was $89.6\%$ (121/135), and the median follow-up duration was 66 months. Among the 135 patients, 4 relapsed in group 1 (5-FU+cisplatin), 7 in group 2 (mitomycin C+oral 5-FU), and 6 in group 3 (oral 5-FU only). The overall survival rate was $89\%$ in group 1, $84\%$ in group 2, and $82\%$ in group 3. There were no differences in the overall survival rates and the disease-specific survival rates among the three groups. Conclusion: Oral chemotherapeutic agents have an acceptable effect for adjuvant chemotherapy compared with intravenous agent. However, a large-scale, prospective, randomized study, including a control group, is needed for an exact evaluation.

제5판 UICC TNM림프절 분류에 따른 pT3pN1 위암 환자에서 림프절의 해부학적인 위치에 따른 2군 림프절 양성의 의의

김용진,김병식,최원용,육정환,오성태,박건춘,Kim Yong Jin,Kim Byung Sik,Choi Won Yong,Yook Jung Hwan,Oh Sung Tae,Park Kun Choon 대한위암학회 2004 대한위암학회지 Vol.4 No.1

Purpose: The most important prognostic factors in gastric cancer is the depth of invasion of the primary tumor and lymph node metastasis. The nodal staging of the 5th edition of the Union Internationale Contrala Cancrums (UICC) TNM classification in 1997 was changed based on the number of metastatic lymph node. We attempted to evaluate the prognostic significance of N2 group metastasis in pT3pN1 gastric cancer patients by comparing two different nodal staging systems. Materials and Methods: A retrospective analysis was made for 352 gastric cancer patients who underwent curative resection, including D2 dissection, from 1991 to 1997 at Asan Medical Center. A clinicopathologic comparison between two groups by using a nodal staging systems was summarized into a table. Cumulative survival rates were calculated by using the Kaplan-Meier method. The difference between the two groups was evaluated by using the log rank test with SPSS 11.5 for Windows Results: There were no statistical differences in clinicopathologic factors. However, there was a significant difference in survival rate between the two groups (P=0.0009). This suggests that N2 group metastasis in pT3pN1 gastric cancer patients has a clinical significance for predicting prognosis. Conclusion: Our results suggest a possibility of prognostic significance of N2 group metastasis. Therefore, anatomical location of the lymph node should be described.

보조생식술 주기에서의 과배란유도를 위한 재조합 황체화호르몬의 효용성

김용진,구승엽,지병철,서창석,김석현,최영민,김정구,문신용,Kim, Yong-Jin,Ku, Seung-Yup,Jee, Byung-Chul,Suh, Chang-Suk,Kim, Seok-Hyun,Choi, Yong-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2008 Clinical and Experimental Reproductive Medicine Vol.35 No.3

가스분무 공정에 의한 고강도 과공정 AI-Si 합금 분말의 제조 및 특성연구 II. 압출재 제조 및 기계적 특성

김용진,김진천,Kim, Yong-Jin,Kim, Jin-Chun 한국분말야금학회 2008 한국분말재료학회지 (KPMI) Vol.15 No.2

The hypereutectic Al-20 wt%Si powders including some amount of Cu, Fe, Mg, Mn were prepared by a gas atomization process. In order to get highly densified Al-Si bulk specimens, the as-atomized and sieved powders were extruded at $500^{\circ}C$, Microstructure and tensile properties of the extruded Al-Si alloys were investigated in this study. Relative density of the extruded samples was over 98%. Ultimate tensile strength (UTS) in stress-strain curves of the extruded powders increased after T6 heat treatments. Elongation of the samples was also increased from 1.4% to 3.2%. The fracture surfaces of the tested pieces showed a fine microstructure and the average grain size was about $1{\mu}m$.

논문(論文) : 『손진인천금방(孫眞人千金方)』과 『비급천금요방(備急千金要方)』 정종옹저편(丁腫癰疽篇)의 차이점(差異點)에 대한 고찰(考察)

김용진 ( Yong Jin1 Kim ) 대한한의학원전학회(구 대한원전의사학회) 2012 대한한의학원전학회지 Vol.25 No.3

Objective : I will study about similarities and differences of furunculus section between Sonjininchungeumbang and Bigeupchungeumyobang. Method : I compare furunculus section of Sonjininchungeumbang with Bigeupchungeumyobang to investigate changes of texts. Result : I can find significant differences of furunculus section between Sonjininchungeumbang and Bigeupchungeumyobang, specially the 3rd prescription of Balbae(發背方第三), the 6th prescription of Pyojeo(표疽方第六). Conclusion : Sonjininchungeumbang is a prescription book which is compiled in Dang dynasty. And Bigeupchungeumyobangis is a revised version of Sonjininchungeumbang which is compiled by the Bureau for Revising Medical Texts(校正醫書局) in Song dynsasty. Bigeupchungeumyobangis shows the added medical knowledge about furunculus in Song dynsasty.

복잡 대동맥 교약증 또는 대동맥 차단증의 일차적 완전교정술에 대한 연구

김용진,전태국,노준량,Kim, Yong-Jin,Jeon, Tae-Guk,No, Jun-Ryang 대한흉부심장혈관외과학회 1995 Journal of Chest Surgery (J Chest Surg) Vol.28 No.7

Between March 1989 and December 1994, one-stage repair was performed for correction of the intracardiac malformations associated with aortic coarctation in 34 patients or interrupted aortic arch in 8 patients via median sternotomy. There were 26 male and 16 female patients, and their body weight ranged from 1.8 to 8kg[mean weight, 4.0$\pm$l.4kg . The age at the operation ranged from 7 days to 18 months [mean age, 3.1$\pm$3.8 months . The repair of aortic coarctation or interrupted aortic arch was performed using extended end-to-end anastomosis in most of the patients[86%, 36/42 , and six patients underwent ductal tissue excision and patch aortoplasty. Intracardiac defects were corrected concomitantly through the right atrium unless the anatomy dictated otherwise. Obstructive outlet septurn was resected whenever necessary. There were seven early deaths[16.8% , and three late deaths with a mean follow-up period of 25 months [range from 1 to 65 months . Three patients were reoperated upon residual subaortic stenosis,stenosis at the RPA origin, and subacute bacterial endocarditis respectively. None showed any significant residual or anastomotic stenosis postoperatively. One stage repair of the aortic coarctation and interrupted aortic arch associated with intracardiac defect leaves no native coarctation shelf tissue or residual hypoplasia in the repaired segment, has low incidence of recurrent or residual stenosis, minimizes reoperation and incisions, and manages arch hypoplasia easily.We conclude that surgical results of one-stage repair for the intracardiac malformation associated with aortic coarctation or interrupted aortic arch are resonable.

개선된 자속구속형의 2차 측 권선 방향과 1차 권선수와 2차 권선수의 변화에 따른 사고전류제한 특성

김용진,두호익,이동혁,한병성,Kim, Yong-Jin,Du, Ho-Ik,Lee, Dong-Hyeok,Han, Byoung-Sung 한국전기전자재료학회 2010 전기전자재료학회논문지 Vol.23 No.9

The improved flux-lock type superconducting fault current limiter (SFCL) is composed of a series transformer and superconducting unit of the yttrium-barium-copper-oxide (YBCO) coated conductor. In this paper, we investigated current limiting characteristics through winding direction of coil 2 and variable number of coil 1 and coil 2 in improved flux-lock type SFCL. The better fault current characteristics and the burden of YBCO coated conductor can be confirmed from the experimental result in the higher turn ratio of coil 1 and coil 2 in the additive conditions. In case of subtractive condition, we can confirm a similar result in the same case of experimental conditions. but the burden of YBCO coated conductor has been increased from an increase in winding numbers of coil 2.