생쥐 난포의 체외배양 중 생식샘자극호르몬에 따른 미세리보핵산 발현 양상

김용진,구승엽,김윤영,오선경,김석현,최영민,문신용,Kim, Yong-Jin,Ku, Seung-Yup,Kim, Yoon-Young,Oh, Sun-Kyung,Kim, Seok-Hyun,Choi, Young-Min,Moon, Shin-Yong 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.4

목 적: 미세리보핵산 (microRNA, miR)은 전사 후 (post-transcriptional) 단계에서 목표 유전자 (target gene)의 발현을 억제하여 세포의 발달과 성장에 중요한 역할을 하는 것으로 알려져 있다. 그러나 난포의 성장과정 중의 miR 발현 양상에 대해서는 잘 알려져 있지 않다. 따라서 존 연구는 생쥐 난포의 체외배양 후 생식샘자극호르몬 (gonadotropin)과 사람융모성생식샘자극호르몬 (hCG) 첨가에 따른 난자와 난구세포에서의 miR 발현 양상을 살펴보고자 수행되었다. 연구방법: 생후 12일된 생쥐 (C57BL6)의 난소 적출 후, 전동 난포 (preantral follicle)를 분리하여 무작위로 $20\;{\mu}L$ 점적의 배양액만 있는 군 (control group), 재조합 난포자극호르몬을 첨가한 군 (FSH group), 재조합 황체형성호르몬을 첨가한 군 (LH group), FSH와 LH를 같이 첨가한 군 (FSH+LH group)으로 나누어 배양하였다. 난포가 충분히 성장하였을 때, 다시 hCG를 첨가한 군 (hCG (+) group)과 첨가하지 않은 군 (hCG (-) group)으로 나누어 hCG (-) group에서 난자와 난구세포를 각각 분리하여 RNA를 추출하였다. 36시간 후, 배란된 난자 난구세포 복합체 (cumulus oocyte complex, COC)에서 난자와 난구세포를 각각 분리하여 RNA를 추출하고, mmu--miR-16, -miR-27a, -miR-126, -miR-721 등의 miR에 대한 primer를 이용하여 실시간 중합연쇄반응을 시행하였다. 결 과: 배란율과 MII 난자 생성율은 다른 군들에 비해 FSH+LH군에서 유의하게 높았다. 각 군내의 난자와 난구세포 사이에서도 miR 발현 양상의 차이가 관찰되었다. 또한, 난자와 난구세포에서의 miR 발현 양상은 각 군간 차이가 있었으며, hCG (+)군과 hCG (-)군간에도 차이를 나타냈다. 결 론: 생쥐 난포의 체외배양 중 난자 및 난구세포에서의 miR 발현 양상은 gonadotropin의 종류 및 난자의 성숙도에 따라 다르다. 이러한 결과는 표적 유전자의 발현에 대한 추후 연구를 통한 확인이 필요할 것으로 사료된다. Objective: MicroRNAs (miR) are known to repress target genes at post-transcriptional level and play important roles in development and maturation of cell. However, the expression profiles of miR during ovarian follicle maturation have not been fully elucidated. Here, we designed this study to investigate the expression profiles of miR in oocytes and granulose cells (G-cells) after in vitro culture according to gonadotropins and adding hCG. Methods: Ovaries from 12-day-old mice (C57BL6) were removed and preantral follicles were isolated and cultured in $20\;{\mu}L$-drop of culture media with supplementation of either rFSH, rLH, or rFSH+rLH. After their full maturation, follicles were incubated with rhCG and rEGF. RNA was isolated from oocytes and G-cells, and real-time PCR were performed with primers of miR known to be expressed in the mouse ovary (mmu-miR-16, -miR-27a, -miR-126, -miR-721). Results: FSH+LH group showed the highest ovulation and MII rates among gonadotropin groups. The profiles of miRs in oocytes and G-cells differed according to gonadotropin groups and adding hCG. The profiles of miRs showed divergent changes between oocytes and G-cells. Conclusion: miR expression profiles are altered by gonadotropins and supplementation of hCG during in vitro maturation of murine follicles. Target gene study must be necessary to validate these findings.

보조생식술 주기에서의 과배란유도를 위한 재조합 황체화호르몬의 효용성

김용진,구승엽,지병철,서창석,김석현,최영민,김정구,문신용,Kim, Yong-Jin,Ku, Seung-Yup,Jee, Byung-Chul,Suh, Chang-Suk,Kim, Seok-Hyun,Choi, Yong-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2008 Clinical and Experimental Reproductive Medicine Vol.35 No.3

자가 정맥 이식을 이용한 수지 접합술

김용진,김형건,김형주,박진철,Kim, Yong-Jin,Kim, Hyung-Kun,Kim, Hyung-Ju,Park, Jin-Chul 대한미세수술학회 1998 Archives of reconstructive microsurgery Vol.7 No.1

We carried out 107 replantations of amputated digits since last 1988. Among them, clean Gillotine amputation was 19 digits, mild crush amputation was 51 digits, severe crush amputation was 28 digits and avulsion type amputation was 9 digits. Vein grafts were used on 24 of these cases. The donor site of the vein graft was the volar aspect of the ipsilateral wrist or lower forearm. The average length of the vein graft was 1.8cm(1.0-3.0cm) and the average diameter of the vein graft was 1.5mm(1.0-2.0mm). Sixty-one of 107 digits were successfully replanted for an 57% success rate. The survival rate of the replantation using the vein graft was 66.7%. We analysed the functional results of the successfully replanted digits according to Nakamura and Tamai's criteria. The functional results of the replantated digits using vein graft were better than that of without using vein graft(more than fair results, 81.2% vs 73.3%). We conclude that the jucidious use of autogenous vein grafts in digital replantation surgery will improve the survival rate and promote the functional result.

장막침윤이 없는 위암환자에서 수술 후 보조적 화학 요법에 대한 전향적 연구 -최종보고-

김용진,김병식,김용호,육정환,오성태,박건춘,Kim Yong Jin,Kim Byung Sik,Kim Yong Ho,Yook Jung Hwan,Oh Sung Tae,Park Kun Choon 대한위암학회 2004 대한위암학회지 Vol.4 No.4

목적: 장막침윤이 없는 위암환자에 있어서 수술 후 서로 다른 보조화학요법이 장기 생존에 미치는 영향에 대해 평가하고자 하였다. 대상 및 방법: 1996년 10월부터 1998년 3월까지 만 15개월간 서울 아산병원 외과에서 근치적 위 절제를 시행한 환자 중, 장막침윤이 없는 317명의 환자를 대상으로 하였다. 이중 수술 후 병기가 IA인 131예, 장막침윤이 있는 34예, 그리고 치료를 거부한 17예를 제외한 135명(1군: 5-FU+cisplatin 정주, 2군: mitomycin C정주+경구용5-FU, 3군: 경구용 5-FU)의 의무기록 분석과 전화추적을 실시하여 재발양상, 생존율, 및 위암관련 사망률을 각 군별로 분석하였다. 결과: 121명에서 추적이 가능해 $89.6\%$의 생존율을 나타냈다. 재발은 1군 4명, 2군 7명, 그리고 3군 6명 이었으며, 세 군의 전체생존율은 1군 $89\%$, 2군 $84\%$, 그리고 3군은 $82\%$이고, 위암관련 사망환자만을 고려한 질병특이 생존율은 1군 $92\%$, 2군 $86\%$, 그리고 3군은 $88\%$로 나타나 이들 세 군간에 전체생존율 및 질병특이 생존율은 차이를 보이지 않았다(전체생존율: P=0.6875, 질병특이 생존율: P=0.7120). 결론: 장막침윤이 없는 위암 환자에서, 치료효과를 향상 시키기 위해 보조요법을 시행할 경우 경구용 항암제를 이용한 방법이면 충분하다고 판단하였다. 그러나 이를 입증하기 위해서는 보조요법을 시행치 않는 대조군을 포함한 대단위 무작위 연구가 필수적일 것이다. Purpose: We reported our preliminary result in 2001. At that time, the follow-up period was too short to evaluate the survival benefit of adjuvant chemotherapy in gastric cancer without serosal invasion. Therefore, we followed those patients for 66 months to determine the long-term effects of adjuvant chemotherapy. Materials and Methods: We analyzed the recurrence pattern, the survival rate, and the disease-specific survival of 135 patients by reviewing their medical records and calling the patients or their relatives. All enrolled patients were included in the intention-to-treat analysis of efficacy. Results: The follow-up rate was $89.6\%$ (121/135), and the median follow-up duration was 66 months. Among the 135 patients, 4 relapsed in group 1 (5-FU+cisplatin), 7 in group 2 (mitomycin C+oral 5-FU), and 6 in group 3 (oral 5-FU only). The overall survival rate was $89\%$ in group 1, $84\%$ in group 2, and $82\%$ in group 3. There were no differences in the overall survival rates and the disease-specific survival rates among the three groups. Conclusion: Oral chemotherapeutic agents have an acceptable effect for adjuvant chemotherapy compared with intravenous agent. However, a large-scale, prospective, randomized study, including a control group, is needed for an exact evaluation.

PMW-PNN-PZT 세라믹스의 Bismuth 치환에 따른 미세구조 및 압전 특성

김용진,류주현,신동찬,Kim, Yong-Jin,Yoo, Ju-Hyun,Shin, Dong-Chan 한국전기전자재료학회 2016 전기전자재료학회논문지 Vol.29 No.6

In this study, in order to develop the composition ceramics for ultrasonic sensor with high $d_{33}*g_{33}$, $Pb_{1-3x/2}Bix(Mg_{1/2}W_{1/2})_{0.03}(Ni_{1/3}Nb_{2/3})_{0.09}(Zr_{0.5}Ti_{0.5})_{0.88}O_3$(PMW-PNN-PZT) system ceramics were prepared using CuO as sintering aids. And then, their microstructure, piezoelectric and dielectric characteristics were systemetically investigated with bismuth substitution. The PMW-PNN-PZT ceramic specimens could be sintered at sintering temperature of $940^{\circ}C$ by adding sintering aids. At x=0.015 specimen, the density, electromechanical coupling factor($k_p$), dielectric constant, piezoelectric constant($d_{33}$) and piezoelectric figure of merit($d_{33}*g_{33}$) indicated the optimal properties of $7.90g/cm^3$, 0.67, 2,511, 628 pC/N, and $17.7pm^2/N$, respectively, for duplex ultrasonic sensor application.

CeMnO₃가 치환된 저온소결 PMW-PNN-PZT 세라믹스의 유전 및 압전 특성

김용진,류주현,Kim, Yong-Jin,Yoo, Ju-Hyun 한국전기전자재료학회 2015 전기전자재료학회논문지 Vol.28 No.3

In this study, $(1-x)Pb(Mg_{1/2}W_{1/2})_{0.03}(Ni_{1/3}Nb_{2/3})_{0.09}(Zr_{0.5}Ti_{0.5})_{0.88}O_3+xCeMnO_3$ (x= 0~0.02) ceramics were prepared by Columbite precursor method. The phase structure, ferroelectric and piezoelectric properties were systematically investigated. It was found that PMW-PNN-PZT possessed superior electrical properties due to its composition close to the MPB (morphotropic phase boundary). Coercive electric field of 10.05 [kV/cm] and density of 7.88 [$g/cm^3$] were obtained when the substitution amount of $CeMnO_3$ is x=0.02. In contrast, specimens with x=0.01 showed the mechanical quality factor($Q_m$) of 1,091 and the electromechanical coupling factor($k_p$) of 0.613.

구성식을 이용한 다짐화강토의 공학적 특성(1)

김용진,이인모,이인근,Kim, Yong-Jin,Lee, In-Mo,Lee, In-Geun 한국지반공학회 1994 지반 : 한국지반공학회지 Vol.10 No.3

풍화화강토는 화강암이나 화강편마암이 풍화된 흙으로 건설현장에서 빈번하게 접하고 있는 우리나라의 대표적인 토질이며, 공기에 노출되거나 물과 접촉하면 강도특성이 쉽게 변화하는 문제성이 많은 토질로 알려져 있다. 이 연구에서는 이러한 풍화화 강토의 역학적 특성을 파악하기 위하여 기원과 구성성분이 다른 안동과 김천에서 풍화화 강토를 채취하여 실내다짐에 의하여 공시체를 제작한 후에 등방압밀배수 및 비배수시험을 실시하였으며, 시험결과를 분석하여 여러가지의 강도특성을 분석하고 Cam-Clay모델에의 적용성을 검토하였다. 안동과 김천시료의 기원은 상이하지만 전단저 항각과 압축특성은 실용적으로 동일하였다. 비체적대 평균유효응력공간에서 정규압축곡선과 한계상태선이 유일한 것으로 나타났으며 응력-변형률 거동특성은 점성토나 모래의 특성과는 다르게 나타났다. 과압밀비가 2이상일 경우에 유효 응력비가 한계상태에 수렴하였지만 응력-변형률 관계에서 최대음력이 나타나지 않았다. 응력경로는 이론적인 Roscoe면 아래로 이동하면서 한계상태에 접근하였다. 정규압밀비배수시험에서 간극수압계수(A,)는 1.3정도이었는데 이것은 전단중에 발생하는 입자파쇄현상에 의한 것으로 판단되었다. 과압밀비가 7정도에서 부(-)의 간극수압과 체적팽창현상이 발생하였다. 구성식에 의하여 실측치와 이론치를 비교한 결과, 배수거동에서는 만족할만한 결과를 구할 수 있었다. Decomposed granite soil is a Granitic Gneiss, and it is a c Korean peninsula. It is known a changed significantly when it is aim of this study is to evaluat utility of the constitutive laws. Firstly, triaxial tests were pe sites prepared by the laborato scrutinized the characteristics results were analysed and the p evaluated. Finally, the predicted Even though the origins of slight difference in the angle of pression line( A) : both soils show In the effective mean normal uniqueness of the Normal Compr The relationships between the the decomposed granite soil tier OCR is larger than 2, the stress stress(MDS) or. even thous moved below the theoretical Ros was found to coincide with the (NC) soils, the pore pressure parameter, A,, increased up to 1.3. This phenomenon might be mainly due to the effect of the particle crushing during shearing, When the OCR value approaches 7, the negative pore pressure is developed in undrained tests and the dilatancy is observed in drained tests. The predicted and the observed behavior of drained tests showed relatively good fitting with the Cam-Clay model.

석유류 세제와 정책과제

김용진,Kim, Yong-Jin 대한석유협회 1985 석유와 에너지 Vol.1985 No.10

프로토콜 검증시스템의 설계 및 구현

김용진,Kim, Yong-Jin 한국전자통신연구원 1989 전자통신 Vol.11 No.4

In this paper, a design and implementation of an efficient protocol verification system named LOVE has been described. The LOVE has been developed specifically for LOTOS. It performs not only protocol syntax validation (PSV) but also protocol functional verification(PFV). The PSV is a test to check if a protocol is free from protocol syntax errors such as deadlocks and livelocks. The PFV confirms whether or not a protocol achieves its functional objectives. For the PSV, the reachability analysis is employed, and the observational equivalence test is used for the PFV. For protocol verification using the LOVE, a schematic protocol verification methodology has been outlined.

한국인의 발꿈치 패드 두께의 분석

김용진,김형직,이광복,Kim, Yong-Jin,Kim, Hyeong-Jik,Lee, Kwang-Bok 대한족부족관절학회 2015 대한족부족관절학회지 Vol.19 No.4

Purpose: The purpose of this study is to provide Korean data on heel pad thickness according to age, gender, underlying disease, occupation, and body mass index (BMI). Materials and Methods: A retrospective study was conducted on 670 patients who underwent foot lateral plain radiography and magnetic resonance imaging (MRI) between January 2010 and July 2014. Through measurements of heel pad thickness, the usefulness and accuracy of foot lateral plain radiography was evaluated, and the mean Korean heel pad thickness in the weight-bearing and non-weight-bearing conditions was also evaluated according to age, gender, underlying disease, occupation, and BMI. Results: The 670 subjects with a mean age of 44 years (range, 12 to 84 years) consisted of 420 males and 250 females. The difference in heel pad thickness between non-weight-bearing foot lateral plain radiography and MRI was 0.69 mm. The heel pad thickness did not show a significant difference with age (p=0.08) and the presence of diabetes (p=0.09). With the increase in the Tegner score, the thickness of the heel pad increased (p=0.035), and subjects with a higher BMI had a thicker heel pad (p=0.03). The compressibility of the heel pad thickness showed no correlation with gender, diabetes, and Tegner score. Compressibility also increased with the increase in age and body weight. Conclusion: The mean Korean heel pad thickness measured through non-weight-bearing foot lateral plain radiography was 18.79 mm. The heel pad thickness increased with increasing BMI; however, age and diabetes did not show significant correlation. The compressibility of heel pad increased with the increase in age.