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인간 무세포성 진피기질 위에 배양한 가토 구강각화상피세포의 중충화와 기저막 형성에 관한 연구
김용덕,안강민,염학렬,정헌종,김성민,장정원,성미애,박희정,황순정,이종호,Kim, Yong-Deok,Ahn, Kang-Min,Yum, Hak-Yeol,Chung, Hun-Jong,Kim, Soung-Min,Jang, Jeong-Won,Sung, Mi-Ae,Park, Hee-Jung,Hwang, Soon-Jung,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.6
To assess the clinical applicability of bio-artificial mucosa which was made with autologous oral keratinocytes and human acellular dermal matrix, the formation of basement membrane and stratification of oral keratinocytes were evaluated. Six New Zealand white rabbits (around 2kg in weight) were anesthetized and its buccal mucosa was harvested (1.0 $\times$ 0.5cm size). Oral keratinicytes were extracted and cultured primarily with the feeder layer of pretreated NIH J2 3T3 fibroblast. These confluent cells were innoculated on the human acellular dermal matrix and cultured in multiple layer by air-rafting method. After 3, 5, 7, 10, 14 days of culture, each cultured bio-artificial mucosa was investigated the number of epthelial layer of by H&E stain and toluidine blue stain. The immuhohistochemical methods were used to evaluate the cell division capacity, the formation of basement membrane, and it's property of specific cells (PCNA, cytokeratin 14, laminin). Transmission electromicroscopy was used for the attachment between cells and matrix with the number of hemidesmosome. In result, the numbers of layer of stratified growth of oral keratinocyte cultured on the human acellular dermal matrix and the number of hemidesomal attachment between epithelial cells and human acellular dermal matrix were similar to the layers of normal oral mucosa after 10 days of culture. The cell division rate, basement membrane formation and proliferation rate increased as culture period increased. With these results, bio-artificial mucosa with autologous oral epithelial cells cultured on the acellular dermal matrix had clinically adaptable properties after 10 days' culture and this new bio-artificial mucosa model with relatively short culture time can be expected clinical applicability.
기계적합금화한 AI-Ti합금의 고온인장특성에 미치는 Zr의 첨가와 등온열처리의 영향
김용덕,원형민,김선진,Kim, Yong-Deok,Won, Hyeong-Min,Kim, Seon-Jin 한국재료학회 1996 한국재료학회지 Vol.6 No.11
Ai-8wt.5(Ti+Zr)합금을 기계적합금화와 열간압출로 제조하여 Ti에 대한 Zr 첨가비와 등온열처리가 고온인장강도 및 변형거동에 미치는 영향에 대하여 조사하였다. Ti에 대한 Zr 첨가량의 비가 증가함에 따라 열간압출 시편의 상온 및 고온강도가 증가하였고, 40$0^{\circ}C$ 및 51$0^{\circ}C$에서 등온열처리에 따른 강도의 감소도 작게 나타났다. 이는 Zr 첨가량이 증가함에 따라 AI 기지와 AI3Ti에 비해 작은 격자간불일치도를 갖는 AI3(Ti+Zr)금속간화합물이 생성되고 고온열처리에 따른 조대화가 억제되었기 때문으로 판단되었다. 합금의 연성은 Zr 첨가량과 등온열처리에 관계없이 10% 이하로 낮게 나타났으며 인장 시험 온도가 고온일수록 취성파괴인 입계파괴가 지배적으로 일어났다. AI-Ti-Zr 합금의 변형에 필요한 활성화에너지는 순수한 AI 기지의 자기확산에 필요한 활성화에너지 142KJ/mol에 비해 573-783K 온도범위에서 1.5-1.8배 높은 값을 보였으며, Ti에 대한 Zr의 첨가량의 비가 증가할수록 보다 높은 값을 나타내었다.
산업단지 입주기업과 비입주기업의 효율성 분석에 관한 연구
김용덕 ( Yong-deok Kim ),조재민 ( Jaemin Cho ) 한국생산성학회 2017 生産性論集 Vol.31 No.4
This research aims to measure the efficiency on firms in the industrial complex from 2013 to 2015 and evaluate the factors to make an effect to the efficiency scores, using Data Envelopment Analysis (DEA) and Tobit regression analysis. The major findings of this study are as follows. The results show that the average efficiency score of the firms located in industrial complex is lower than non industrial complex firms and the primary reason to cause their inefficiency is Technical Efficiency (TE) and Pure Technical Efficiency (PTE). Tobit Regression analysis shows that technical efficiency is higher for non industrial complex firms than for industrial complex firms. Therefore, it can be seen that the efficiency of the companies residing in industrial complexes is rather weaker than that of non-industrial complex firms. Analysis by industry, industrial complex firms are inefficient compared to non industrial complex firms in the manufacturing industry and construction industry. In particular, the longer the workforce and the greater the CEO age, the more negative the effect on the efficiency. This suggests that the synergy among companies has weakened as industrial complexes have become obsolete and time-consuming businesses have diversified. in the case of Namdong and Banwol, firms that are engaged in machinery, electricity, and electronics, which are the main flagship industries, have gradually decreased in accordance with the changes in industrial structure. Instead, the number of simple processing companies such as beverages and foodstuffs has increased, and the number of small sized enterprises, such as construction and wholesale/retail businesses, which do not require technology development, is increasing. Indeed, the proportion of companies with less than 50 employees in 2016 exceeded 95% in the case of the southeast and 96% in the case of the Banwol. In addition, the average number of Korean industrial complexes is 16.3%, compared to 10.8% in Namdong and 13.3% in Banwol. This implies that there is a possibility that industrial complex firms lose their competitiveness compared to non-industrial complex firms due to the decrease in the role and effect of industrial complexes, encourage the departure of competent industrial complex firms and leading companies, and accelerate the weakening of competitiveness. This study is the first attempt to analyze efficiency and analyze the determinants of industrial complex firms. In particular, efficiency is compared and distinguished by type of industry and presence of industrial complexes. As a result, it is necessary to review the roles and functions of industrial complexes.
김용덕(Yong-Deok Kim),박성희(Sung-Hee Park),정인교(In-Gyo Chung),손우성(Woo-Sung Son),김욱규(Uk-Kyu Kim),신상훈(Sang-Hun Shin) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.4
We have examined 20 tongue-tie patients who had been operated the lingual frenectomy in Dept. of Oral & Maxillofacial surgery, Pusan National University for the effect of the lingual frenectomy on the tongue motion and speech, and divided patients into groups by age. Each group was separated as follows ; the age of 5-6, 7-9, 10-12, 13-15, and 16-18. We measured the frenal lengh, the range of tongue motion and evaluated the speech pre- and postoperatively. The preoperative patient groups have larger tongue tips and smaller lingual frenums than normal ones. In the preoperative patient group, there was significant obstacle of the protrusive and superior movement of the tongue. The exception was the laterotrusive movement on both sides. There was the lower value of the Picture Consonant Articulation Test(PCAT) in the preoperative 5 to 6 yearold group compared with normal group. In other age groups, there was no significant difference. The range of postoperative PCAT in all age groups has become as similiar as that of normal group. The 5 to 6 year-old group which had significant difference in PCAT was improved in PCAT. In conclusion, we propose that the PCAT as well as anatomical tongue size and functional tongue movement is the basis of the indication of lingual frenectomy and the 5-6 year-old is the optimal time of the lingual frenectomy for improvement of the tongue movement and the speech.