밤나무 종실에 발생하는 탄저병균의 분리 및 친환경적 방제

김영재,강길남,김종우,김영명,이상현,홍석일,이재준,이현수,김영권,박인서,조용구,Kim, Young-Jae,Kang, Kil-Nam,Kim, Jong-Woo,Kim, Young-Myung,Lee, Sang-Hyeon,Hong, Suk-Il,Lee, Jae-Jun,Lee, Hyun-Su,Kim, Young-Kwon,Park, In-Seo,Cho, Yong-Gu 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.4

본 실험에 사용한 C. gloeosporioides는 밤나무 재배단지에서 탄저병 증상을 보이는 밤나무 종실로부터 분리하였고, ITS 영역 유전자의 염기서열 및 형태하적 분석을 통하여, C. gloeosporioides로 동정하였다. 병원성 검정실험에서, 80% Eucalyptus oil 및 B. subtilis GG95 10배액 처리구가 C. gloeosporioides에 대한 항균효과가 우수하였고, 최종배합은 Eucalyptus oil 40%, B. subtilis GG95 40% 외 제제화용 첨가제 20%로 하였다. 2012년도 포장실험에서, 자봉 품종 시제품 500배 4회 처리구의 방제가는 무처리구 대비 60.9%로 조사되었고, 2013년도 포장실험에서, 자봉 품종의 방제가는 250배 처리구가 71.2%로 가장 높았다. 조생종 및 중생종보다 만생종에서의 방제가 69.8%로 가장 높은 것으로 보아, 착과이후 종실이 작을 때부터 약제 처리를 하면, 방제가를 높일 수 있을 것으로 기대된다. 포장 500배 4회 처리후 수확물인 조생종 단택에서는 $4^{\circ}C$ 저장에서의 방제효과는 약 61.9%로 조사되었고, $25^{\circ}C$ 저장에서의 방제효과는 63.2%의 방제가로 조사되었고, 실내 단태 품종에 대한 방제가는, 250배에서 81.16%, 500배에서 75.36%로 측정되었으나, 1000배의 효과는 낮은 편이므로, 최소한 500배 이상의 처리가 필요시 된다. 상기의 결과를 바탕으로 밤나무 종실에 발생하는 탄저병을 효율적으로 방제하기 위해서는 착과 이후 개발제품을 500배 희석농도로 4회 경엽처리하고 수확후 250배로 침지 처리한 후 저장하는 것이 가장 효율적인 방법으로 판단된다. In order to acquire morphological characteristics and genetic characteristics of pathogen that causes anthrax to chestnut, anthrax was separate and identified in Gongju, chungnam chestnut plantation. Antagonistic microorganisms and plant extracts were selected for control of anthrax. Medium maturing variety treatment of 250 dilution fold in field was control at 71.2% and treatment of 500 dilution fold was control at 64.4% and treatment of 1000 dilution fold was control at 40.7%. Storage control value of Jabong in $25^{\circ}C$ after treatment in field is 61.7% at 250 dilution fold, 62.8% at 500 dilution fold, 40.9% at 1000 dilution fold treatment.

위암의 연령에 따른 차이

김영재(Young Jae Kim),이영철(Young Chul Rhee),이만길(man Kill Lee),차호(Ho Cha),정영채(Young Chae Jung) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Comparative analytic review on clinical, endoscopic and histopathological findings was done in 13 cases of young age (under 29), 446 cases of mid age (30~69) and 39 cases of old age (above 70) stomach cancer who were visited Department of Internal Medicine of Masan Koryo General Hospital from March 1981 to July 1985. The following results were obtained: The peak of age incidence was the 5th decade amounting to 36.8%. The ratio between male and female was 2.6:1. The main chief complaint of each group was epigastric pain and it was followed by epigastric fullness. The most patient had the duration of symptom from 3 months to 6 months. For old-age group, mid-age group, major site of lesion in the antrum, meanwhile body for young-age group. The most frequent type of gastric cancer was type III in each group by Borrmanns classification. For each group, adenocarcinoma was major cases in the view of histopathological findings, and incidence of well differentiated type adenocarcinoma were lower in young-age group than others.

대학 아마추어 골프 선수들의 재미요인이 운동몰입에 미치는 영향

김영재(Young Jae Kim) 한국사회체육학회 2011 한국사회체육학회지 Vol.0 No.46

The purpose of this study was to the Relationship between Participation in collegiate golf players, and their enjoyment with commitment. Subjects for this study consisted of collegiate golf players. A total of 177 participants, 92 males and 85 females players and the major rated themselves as having good health and coming from a middle or upper class. Also, I revised the questionnaire which Kim Byoung-Joon(1999), Kim Young-Jae(2006) had used, and applied it to estimate the fun elements. Moreover, I revised the survey which Jeong Yong-Gak(2004), Park, Soon-Taek(2007) developed based on the current situation in Korea from the basis of Scanlan(1993)`s ESCM and applied it to estimate the involvement experience. And then I let them write out the questionnaires in self-administration method and collected the questionnaire The collected materials were then analyzed into both cause and reliability analyses and depending on the purpose of the analysis, correlation analysis and multiple regression analysis were implemented by using SPSS 13.0. The conclusion from the material analysis based on the above processes is as follows. First, it turned out that the fun element and sports commitment element have significant relevancy. Second, the fun elements based on the participation of the golf play which are victory and competition, club atmosphere elements, the perception of competency, practice and benefits excluding the support of the family, and the have significant effect on the collegiate golf players commitment.

밤재배원에서 유살등 구조 및 램프의 종류가 해충 유인력에 미치는 영향

김영재,김현경,김길하,Kim, Young-Jae,Kim, Hyun Kyung,Kim, Gil-Hah 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.3

밤재배원에서 램프광선을 이용한 방제기기(A, B, C형)를 개발하여 램프의 종류에 따라 유인된 곤충의 포획력을 조사하였다. A형 기기에서 수은 램프를 이용하여 포획된 곤충은 125종이었고, 이 중 밤나무 해충은 23종만을 종 동정하였다. B형과 C형의 기기에 컴팩트형광 램프를 부착하여 포획성능을 검정한 결과 B형기기에서는 딱정벌레류(83%), C형 기기에서는 나비류(73%)의 유인효과가 높은 것으로 나타났다. B형 기기를 이용한 밤바구미의 성충에 대한 램프의 유인 포획력을 조사한 결과에서는 수은투명 램프(평균 9.8마리)에서 유인포획 효과가 가장 높았다. C형 기기를 이용한 복숭아명나방의 성충에 대한 유인 포획력을 조사한 결과에서는 컴팩트형광 램프(평균 10.2마리)를 이용한 기기에서 포획수가 가장 많았다. The effects of insect capture were studied in a chestnut orchard using three different light traps (A, B, and C type) with various lamps. The mercury lamp trap captured 125 insect species, out of which 115 were chestnut pests. The B and C type light traps, comprising a Dulux-EL white lamp, were examined for their capturing ability. The type B trap attracted Coleopteran insects (83%), while type C captured Lepidopteran insects (73%). The mercury clarity lamp along with the type B light trap was most effective in attracting Curculio sikkimensis adults (mean, 9.8 adults), while the Dulux-EL lamp captured the highest number of Dichocrocis punctiferalis adults (mean, 10.2 adults) using the type C light trap. These results suggest that selection of the appropriate types of light traps and lamps based on the target pest species is critical in ensuring effective and eco-friendly control of the pest population.

가족캠핑 참여에 따른 가족건강성과 지속행동요인이 행동의도에 미치는 영향

김영재(Young Jae Kim) 한국여가레크리에이션학회 2013 한국여가레크리에이션학회지 Vol.37 No.4

This study aims to predict continuous behavioral intentions and to examine the ultimate value of family by applying the Theory of Planned Behavior(TPB) to family camping participants, based on the theory of family strength which hasn`t been handled, e ven though it can be acquired from leisure activities. This study targeted 407 camping participants. 168 participants(41.3%) were in their thirties on camping. This study adopted the scale of TPB used in the study by Azjen(1985) and Kim, young-jae(2001), and family strength was based on the studies by Lim, Jeong-ah and Lee, In-soo(2006). For analysis, frequency analysis, MANOVA, reliability analysis, factor analysis and regression analysis. As a result, there were statistically significant differences between family strength and TPB factors depending on the spending on camping and participation period. According to the correlation analysis and multiple regression analysis to investigate the correlation between family strength and TPB factors depending on the participation intentions of camping participants, also, the majority of factors showed a correlation, and family problem-solving, subjective norms, control factor and mental attitude factor affected their participation intentions.

떨림과 경직이 발생한 척추수술후증후군 환자에서 척수자극술에 의한 치료 경험 -증례 보고-

김영재,김명훈,임세훈,이정한,이근무,정순호,최영균,신치만,Kim, Young Jae,Kim, Myoung Hun,Lim, Se Hoon,Lee, Jeong Han,Lee, Kun Moo,Cheong, Soon Ho,Choe, Young Kyun,Shin, Chee Mahn 대한통증학회 2006 The Korean Journal of Pain Vol.19 No.1

Tremor is a rhythmic, involuntary and oscillatory movement of body parts, and it is the most common movement disorder. Spasticity is also one of the movement disorders that is commonly accompanied with Complex Regional Pain Syndrome; however, the basic nature of spasticity has not yet been proved. A 25-year-old male patient had two operations and he was being treated because of a back injury that occurred 4 years ago. He suffered from pain, tremor and spasticity on both his lower legs, and his symptoms were diagnosed as failed back surgery syndrome. The tremor and spasticity were aggravated despite of continuous treatments. We then treated him with spinal cord stimulation. His pain, tremor and spasticity disappeared after spinal cord stimulation.

밤바구미 방제를 위한 온도 및 시간별 침지처리 효과와 종실 변화

김영재,김현경,이가순,김길하,Kim, Young-Jae,Kim, Hyun Kyung,Lee, Ka-Soon,Kim, Gil-Hah 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.4

밤재배원에서 발생하는 밤바구미를 물리적으로 방제하기 위하여 침지처리 온도 및 시간별로 유충에 대한 방제효과와 밤 종실에 미치는 성분변화 등을 조사하였다. 밤 종실내의 밤바구미 유충을 방제하기 위하여 다양한 온도조건(30, 35, 40, 45, $50^{\circ}C$)과 시간별(1, 2, 3, 5, 7, 10, 15, 22, 24시간)로 처리하여 방제활성을 조사하였다. 침지처리에 의한 밤 종실의 변화 조사로는 밝기(lightness), 적색도(redness), 황색도(yellowness) 등의 색도의 변화와 발아율, 부패율과 같은 생리적 변화, 경도(hardness)와 같은 물리적 변화, 수분함량과 유리당, 탄닌과 같은 성분변화를 조사하였다. 침지처리를 이용한 밤바구미 유충의 방제 효과는 $30^{\circ}C$에서 2시간 후부터 70%이상의 살충효과를 보였으며, 7시간 후부터는 100% 방제효과를 보였다. $40^{\circ}C$에서는 2시간 후부터 90%의 효과를 보였으며, $45^{\circ}C$ 이상에서는 침지 처리 1시간 후부터 90%이상의 살충효과를 나타내었다. 밤 종실에 대한 색도의 변화는 $30^{\circ}C$와 $35^{\circ}C$에서는 큰 유의차가 없었으나 $40^{\circ}C$ 이상의 온도에서는 시간이 증가할수록 밝기, 적색도, 황색도에 영향을 미쳤다. 발아율의 경우 침지처리에 대한 영향은 크지 않은 것으로 조사되었으나, $40^{\circ}C$ 이상의 온도조건에서는 침지시간이 증가할수록 부패율이 증가하는 것으로 조사되었다. 경도는 온도와 침지처리 시간이 증가할수록 감소하였다. 침지처리에 의한 종실 성분의 변화는 온도와 침지처리 시간이 증가할수록 유리당함량이 감소하였다. 이상의 결과들은 적절한 온도와 시간조건에서의 침지처리가 밤바구미 유충 방제에 효과적이며, 밤 종실의 상품성에도 큰 영향을 미치지 않음을 보여주었다. 따라서 본 연구결과는 밤 종실의 침지조건을 선별하는데 기초자료로 사용될 수 있을 것으로 기대된다. To control populations of the chestnut weevil, Curculio sikkimensis Heller, the effects of various immersion temperatures and times on the chestnut weevil were tested. The effects of immersion treatments on chestnut fruits were also analyzed. The mortality levels of C. sikkimensis larvae were evaluated at various temperatures ($30^{\circ}C$, $35^{\circ}C$, $40^{\circ}C$, $45^{\circ}C$, and $50^{\circ}C$) and time intervals (1, 2, 3, 5, 7, 10, 15, 22, and 24 h). Changes in the chestnut fruit due to the immersion treatment were measured in terms of color (lightness, redness, and yellowness), physiological effects (germination rate and decay rate), hardness, and change in constituent properties (moisture content, free sugar content, and tannin content). Mortality levels of C. sikkimensis larvae after immersion treatment at $30^{\circ}C$ were over 70% and 100% after 2 h and 7 h, respectively. Color (lightness, redness, and yellowness) of the chestnut fruits was not significantly different at $30^{\circ}C$ and $35^{\circ}C;$ however, the color was affected at temperatures over $40^{\circ}C$. The germination rate of the fruit was not affected by the immersion treatments, but the decay rate at $40^{\circ}C$ increased with an increase in immersion time. The hardness of the fruits decreased with an increase in immersion times and temperatures. These results can be used in the future for developing methods for the control of chestnut weevil populations.

치과 진료에 비협조적인 주의력 결핍 과잉행동 장애 증후군 환자에서의 전처치로서의 미다졸람 케타민의 근육투여

김영재,신터전,현홍근,김정욱,장기택,이상훈,김종철,김현정,서광석,이정만,신순영,Kim, Young-Jae,Shin, Teo Jeon,Hyun, Hong-Keun,Kim, Jung-Wook,Jang, Ki-Taeg,Lee, Sang-Hoon,Kim, Chong-Chul,Kim, Hyun-Jeong,Seo, Kwang-Suk,Lee, Jung-Man,Shin, 대한치과마취과학회 2012 Journal of Dental Anesthesia and Pain Medicine Vol.12 No.2

Attention deficit hyperactivity disorder (ADHD) is characterized by inattention, impulsivity, and hyperactivity. Given high incidence of ADHD, many children with ADHD is likely to present for anesthesia. This case report suggests intramuscular premedication as an alternative method for anesthetic induction. A 9-year-old male patient with ADHD was transferred for dental treatment under general anesthesia. The patient refused to go into dental clinic office. Oral midazolam was given to the patient, however, he was resistant to take midazolam via oral route. Instead, we administer midazolam and ketamine via intramuscular route. After less than 10 miniutes, the patient became drowsy and was transferred to dental chair. Intravenous access and mask inhalation was possible. The patient received dental treatment under general anesthesia and recovered in a non-complicated way. In this case, intramuscular sedation with midazolam and ketamine was used as a premedication in highly uncoopearive patient refused to take oral sedative medication.

보리차(Hordeum vulgare var, hexastichon)가 수산화인회석에 대한 우식유발성 세균의 부착에 미치는 영향

김영재,김종철,김각균,Kim, Young-Jae,Kim, Chong-Chul,Kim, Kack-Kyun 대한소아치과학회 2002 大韓小兒齒科學會誌 Vol.29 No.4

Effect of the roasted barley tea in commercial markets on the adherence to the saliva-coated hydroxyapatite(HA) beads and the cell surface hydrophobicity of Streptococcus mutans and Streptococcus sobrinus as cariogenic microorganism was examined in vitro. Adherence activity and hydrophobicity in bacteria tested in all the barley tea samples decreased and the values were different according to the type of tea and the type of treatment. The inhibition of bacterial adsorption to HA beads suggest that barley tea active molecules as catechins and melanoidins may adsorb to a host surface, preventing the tooth receptor from interacting with any bacterial adhesions. The obtained results showed that the barley tea may inhibit bacterial adherence, the first step of the pathogenesis of dental caries in which these microorganism are involved. 시판되는 보리차 3종(티백형 2종 알곡형 1종)이 우식유발 세균인 Streptococcus mutans와Streptococcus sobrinus가 타액피복 수산화인회석에 부착되는 정도와 이들 세균의 체표소수성에 미치는 영향에 대해 평가하였다. 모든 실험군에서 보리차로 처리하였을 때 수산화인회석에의 부착능과 세균의 체표소수성은 감소하였고 이 값은 보리차의 종류와 처리방법에 따라 상이하였다. 이러한 결과는 보리차에 함유된 활성성분인 catechins와 melanoidins의 영향 때문인 것으로 추측되며 보리차로 전 처리한 수산화인회석에 세균이 부착하는 정도가 감소한 것은 활성물질이 흡착되어 치아표면의 수용체가 세균의 부착소와 작용하는 것을 방해함으로써 치아우식증 병인론의 초기 단계인 부착에 영향을 미칠 수 있다는 것을 의미한다. 본 연구결과 보리차가 우식유발 세균의 주요 독력인자 중 하나인 부착능을 억제하므로 보리차가 이러한 세균이 일으키는 치아우식증을 억제할 수도 있다는 가능성을 보여준다.

항우식작용을 갖는 여러 항균물질의 조합에 따른 분할 저해 농도(FIC ; Fractional inhibitory concentration) 지수에 대한 연구

김영재,김종철,김각균,Kim, Young-Jae,Kim, Chong-Chul,Kim, Kack-Kyun 대한소아치과학회 2002 大韓小兒齒科學會誌 Vol.29 No.4

임상에서 사용하고 있는 8종의 항우식 작용을 갖는 항균물질의 조합이 대표적 우식유발 세균인 Streptococcus mutans Ingbritt와 Streptococcus sobrinus 6715-7의 성장에 미치는 영향을 조사하고자 세균별로 28개 조합의 각 항균물질에서 최소 저해 농도와 분할 저해 농도 지수를 구하여 여러 기준에 따라 상승작용, 길항작용, 무관함, 부분적 상호작용 등을 각각 평가하였다. 미국 미생물 학회의 지침에 따라 분류하면 약 34%의 조합에서 상승작용이 관찰되었고 Berenbaum의 분류에 따르면 약 82%에서 상승작용을 갖는다고 해석할 수 있었다. 또한 Isenberg가 정의한 부분적 상승작용은 총 조합수의 절반에서 관찰되었다. 분류기준에 따라 다양한 결과를 얻을 수 있었으나 두 가지 항균물질의 조합이 우식유발 세균을 억제하는데 상승작용을 나타내는 경향이 존재함을 관찰할 수 있었고 따라서 우식유발세균을 억제하는데 있어서 항균물질의 단독 사용보다 세균의 생태와 대사의 여러 부분에 영향을 미치는 항균물질의 조합은 유용할 수 있다는 것을 알 수 있었다. The effect of combinations of 8 antimicrobial agents on the growth of Streptococcus mutans and Streptococcus sobrinus was investigated with the minimum inhibitory concentration and fractional inhibitory concentration(FIC) and ${\Sigma}FIC$ index. According to the ${\Sigma}FIC$ values by. The American Society for Microbiology and Berenbaum, Approximately 34% and 82% of the combinations were synergistic respectively. Partial synergy described by Isenberg was also observed in the half of the combinations. There was a tendency for additive antimicrobial effect against cariogenic bacteria though the test results showed difference according to the applied values. It may be beneficial to use combined antimicrobial agents that have various activities against an ecosystem and metabolism of bacteria than using individual agents.