골막기원세포에서 strontium에 의한 조골세포 표현형의 활성

김신원,김욱규,박봉욱,하영술,조희영,김정환,김덕룡,김종렬,주현호,변준호,Kim, Shin-Won,Kim, Uk-Kyu,Park, Bong-Wook,Hah, Young-Sool,Cho, Hee-Young,Kim, Jung-Hwan,Kim, Deok-Ryong,Kim, Jong-Ryoul,Joo, Hyun-Ho,Byun, June-Ho 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.3

This study investigated the effects of strontium on osteoblastic phenotypes of cultured human periostealderived cells. Periosteal tissues were harvested from mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the periostealderived cells were further cultured for 28 days in an osteogenic induction DMEM medium supplemented with fetal bovine serum, ascorbic acid 2-phosphate, dexamethasone and at a density of $3{\times}10^4$ cells/well in a 6-well plate. In this culture medium, strontium at different concentrations (1, 5, 10, and 100 ${\mu}g$/mL) was added. The medium was changed every 3 days during the incubation period. We examined the cellular proliferation, histochemical detection and biochemical measurements of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and von Kossa staining and calcium contents in the periostealderived cells. Cell proliferation was not associated with the addition of strontium in periosteal-derived cells. The ALP activity in the periosteal-derived cells was higher in 5, 10, and 100 ${\mu}g$/ml strontium-treated cells than in untreated cells at day 14 of culture. Among the strontium-treated cells, the ALP activity was appreciably higher in 100 ${\mu}g$/ml strontium-treated cells than in 5 and 10 ${\mu}g$/ml strontium-treated cells. The levels of ALP and osteocalcin mRNA in the periosteal-derived cells was also higher in strontium-treated cells than in untreated cells at day 14 of culture. Their levels were increased in a dose-dependent manner. Von Kossa-positive mineralization nodules were strongly observed in the 1 ${\mu}g$/ml strontium-treated cells at day 21 and 28 of culture. The calcium content in the periosteal-derived cells was also higher in 1 ${\mu}g$/ml strontium-treated cells at day 28 of culture. These results suggest that low concentration of strontium stimulates the osteoblastic phenotypes of more differentiated periosteal-derived cells, whereas high concentration of strontium stimulates the osteoblastic phenotypes of less differentiated periosteal-derived cells. The effects of strontium on osteoblastic phenotypes of periosteal-derived cells appear to be associated with differentiation-extent.

서울시 마을마당의 이용후 평가

김신원,허준,박율진,Kim, Shin-Won,Heo, Jun,Park, Yool-Jin 한국조경학회 2000 韓國造景學會誌 Vol.27 No.5

The purpose of this study is to provide the basic data for planning and designing Village Parks in Seoul in the future, which result from analyzing post-occupancy evaluation. For conducting this study, 10 Village Parks among 45, built between 1996 and 1997, were selected and analyzed. The results of this study are as follows. The degree of satisfaction of 10 Village Parks in Seoul has been evaluated on a scale of 10, to be a 6.68 average. This result means that the most respondents are satisfied with the existing Village Parks in Seoul. The correlation of the degree of satisfaction with major planning factors for 3 types of Village Parks shows that accessibility is the most important factor. As for future directions of planning and designing Village Parks, many kinds of sitting, leaning and resting place need to be provided. It is also necessary to improve the visual quality of the parks with various spatial elements. The research also shows that various facilities for people's use should be provided in Village Parks.

형태주제 분석을 통한 마을마당의 설계특성 -서울시의 사례-

김신원,허준,Kim, Shin-Won,Heo, Jun 한국조경학회 2000 韓國造景學會誌 Vol.28 No.2

In this study, design characteristics of Village Parks in Seoul are investigated through an analysis of structuring themes expressed in spatial design. The goals and objectives of this study are: to emphasize the significance of design themes; and to demonstrate an approach to the design of landscapes through an analysis of structuring themes. For conducting this study, geometric forms and naturalistic forms were examined first. That is, the following 15 structuring themes were examined, as guiding themes: the 90$^{\circ}$rectangular theme; the 45$^{\circ}$/90$^{\circ}$angular theme; the 30$^{\circ}$/60$^{\circ}$ angular theme; circles on circle; concentric circles and radii; arcs and tangents; circle segments; the ellipse; the spiral; the meander; the free ellipse and scallops; the free spiral; the irregular polygon; the organic edge; and clustering and fragmentation. Forty five Village Parks in Seoul, built between 1996 and 1997, were analyzed through these 15 structuring themes. An analysis of Village Parks was conducted by the following two categories: land shape and structuring themes; and design directions and structuring themes. The research results are as follows; 1) Geometric forms are more frequently applied than naturalistic forms. 2) Regardless of land shape, geometric forms derived from simple primary shapes, the square and the circle, are frequently used. And the meander and the irregular polygon among naturalistic forms are frequently used. 3) Regarding design directions and structuring themes, design concepts, symbolic meanings and spatial forms are, to some degree, integrated. 4) The spiral is not applied among geometric forms. And the meander and the irregular polygon, as naturalistic forms, are frequently utilized. Research findings obtained from this study could be used in the designing of future Village Parks. For a profound study, future, research is needed in two-dimensional plans and three-dimensional elements of Village Parks.

지역 교통계획과정의 개관

김신원,Kim, Shinwon 대한교통학회 2005 교통 기술과 정책 Vol.2 No.2

지역교통계획과정에서 중요한 요소의 하나는 교통계획과 실행을 위한 관할구역 판단에서의 일관성인데 각 관할기관이 그 관할구역의 범위 안에서만 교통시설에 대한 권한을 갖는다. 계획과정에서 그러한 일관성을 획득하기 위해서는 MPO들이 협력, 계속성, 종합의 3C(cooperation, continuing, cooperative)와 종합적이고 일관성 있는 교통계획과정(요구되긴 하나 연방정책이나 프로그램에 의해 꼭 제약받지는 않는)을 갖는 것이 필수 불가결하다. MPO들은 도시화 지역을 위한 교통계획과 프로그램을 개발하는데 있어 주와 지역도시들, 관계기관들과 협력적이어야 한다. 이 교통계획과정은 지역도시와 군들의 종합적으로 계획된 개발과 일관성 있는 계획과 프로그램들로 결과되어 진다. 이 협력적인 교통의사결정과정은 멤버인 관할기관이 그 자격을 위한 지역교통이슈와 계획교통개선을 하기 위한 포럼을 제공한다. 각 도시와 군의 레벨에서, 계획 부서의 교통계획가들이 지역자본시설계획(Capital Facility Plan, CFP)과 지역 교통개선프로그램(Transportation Improvement Program, TIP)을 통해 계획과정을 수행하는데 반해, 공공사업(Public Works)부서의 교통기술자들은 예측되는 혹은 예상되는 교통수요를 맞추는 시설을 설계하고 건설하는 일을 하도록 되어 있다. MPO 스태프들은 지역도시와 군들에 지역맥락과 연방정책 및 프로그램들의 가이드 내에서 종합적이고 일관성 있는 교통자료와 정보를 제공하도록 되어 있다. MPO들은 특히 지역교통수요분석과 예측에서 그러한 전문성이 없는 도시와 군들에 기술적 조력을 제공하도록 되어 있다. MPO의 지역교통계획에 대한 원칙과 가이드는 도시들, 군들, 다른 기관들의 참여(상향식 계획과정인 공공참여를 포함하여)에 의해 개발되어진다. 모든 참여자들은 지역교통 계획에서 지역 목적과 목표를 달성하기 위해 함께 일하게 된다. This is to introduce and overview regional transportation planning processes in the southwest region of the State of Washington, USA. First, significant roles of Growth Management Act (GMA) of the State of Washington in transportation planning are examined. Major functions and roles of Southwest Washington Regional Transportation Council (RTC) as an MPO in the southwest region of the State of Washington, are reviewed mainly from the regional transportation planning perspectives by examining regional transportation planning programs and coordination among the state and local cities and counties. Finally, processes and roles of Metropolitan Transportation Plan (MTP) are discussed. Regional transportation planning processes in a case of the southwest region of the State of Washington may be considered as a reference in regional transportation planning efforts in Korea.

단원 김홍도 회화의 이해를 기초로 구상한 안산 타운하우스 외부 공간 디자인

김신원(Shin Won Kim),서희경(Hee Kyung Suh) 한국디자인문화학회 2008 한국디자인문화학회지 Vol.14 No.3

Townhouse enjoys an increasing importance and attention in housing market in recent days. This study tries to draw this attention into propose a design of an outside space on a townhouse being planned in Ansan city by developing an understanding of a great master of the Joseon dynasty for his creative style of painting and extraordinary endowments. The study, first, examines the artist himself, an outward appearance, characteristics and the art works of Danwon(Pen name號, Birch Garden) Kim, Hong Do, the artist of the Joseon dynasty. The ancient Oriental Fine Arts share a latent spirit of the Cosmic Dual Forces and the Five Primary Substances(陰陽五行) principle, a view of life in Eastern world which affects daily human lives and provides wisdom as living guidelines being closely related to human history. Therefore, the art works of Kim, Hong Do, sharing the very exact spirit as well are interpreted on its grounds and included as properties of a design process. Further, he is renowned and appraised that his art works represented the spirit of his time but also the lives of ordinary people of Korea today because they revealed the aspects of typical Koreans. Thereby, the principal factors of conceptual design drawn out of the masterpieces, which share a view of life in Eastern world could embody an identical constituent and are comprehended in a design process by involving creative reproduction. At close, a design of an outside space(五方位, 東·西·南·北·中央) is proposed one step forward via applying a conceptual foundations of a design concept from the findings of the previous phase. The positive concept realized in the design plan is able to find its way to ascertain a design customized to fit our own preference. The aim of this study is to develop a design concept to approach a design process and definitively, to cope with a planning of the townhouse by means of interpreting important masterpieces of Danwon Kim, Hong Do. The significance of this study is that a concept development of a design process could grow out of the art forms of manifolds. However, for a limited resource, lack of expertise, little hands-on experience provided in this particular research only could allow the suggestion in a qualified sense.

혈관내피유사세포 채취의 원천으로 골막의 활용

박봉욱,김신원,김욱규,하영술,김진현,김덕룡,성일용,조영철,손장호,김종렬,변준호,Park, Bong-Wook,Kim, Shin-Won,Kim, Uk-Kyu,Hah, Young-Sool,Kim, Jin-Hyun,Kim, Deok-Ryong,Sung, Iel-Young,Cho, Yeong-Cheol,Son, Jang-Ho,Kim, Jong-Ryoul,Byun, 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.5

Purpose: The periosteum is a well-known source of osteogenic precursor cells for tissue-engineered bone formation. However, cultured endothelial or endothelial-like cells derived from periosteum have not yet been investigated. This study focused on endothelial-like cell culture from the periosteum. Methods: Periosteal tissues were harvested from the mandible during surgical extraction of lower impacted third molars. The tissues were treated with 0.075% type I collagenase in phosphate-buffered saline (PBS) for 1 hr at $37^{\circ}C$ to release cellular fractions. The collagenase was inactivated with an equal volume of DMEM/10% fetal bovine serum (FBS) and the infranatant was centrifuged for 10 min at 2,400 rpm. The cellular pellet was filtered through a $100{\mu}m$ nylon cell strainer, and the filtered cells were centrifuged for 10 min at 2,400 rpm. The resuspended cells were plated into T25 flasks and cultured in endothelial cell basal medium (EBM)-2. Results: Among the hematopoietic markers, CD146 was more highly expressed than CD31 and CD34. The periosteal-derived cells also expressed CD90 and CD166, mesenchymal stem cell markers. Considering that the expression of CD146 was constant and that the expression of CD90 was lower at passage 5, respectively, the CD146 positive cells in passage 5 were isolated using the magnetic cell sorting (MACS) system. These CD146 sorted, periosteal-derived cells formed tube-like structures on Matrigel. The uptake of acetylated, low-density lipoprotein, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI-Ac-LDL) was also examined in these cells. Conclusion: These results suggest that the CD146-sorted positive cells can be referred to as periosteal-derived CD146 positive endothelial-like cells. In particular, when a co-culture system with endothelial and osteoblastic cells in a three-dimensional scaffold is used, the use of periosteum as a single cell source would be strongly beneficial for bone tissue engineering.

배지 성분에 따른 인간 지방조직기원 CD146 양성 혈관내피세포의 증식 및 기능의 평가

박봉욱,하영술,김진현,조희영,정명희,김덕룡,김신원,김욱규,김종렬,변준호,Park, Bong-Wook,Hah, Young-Sool,Kim, Jin-Hyun,Cho, Hee-Young,Jung, Myeong-Hee,Kim, Deok-Ryong,Kim, Shin-Won,Kim, Uk-Kyu,Kim, Jong-Ryoul,Byun, June-Ho 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.6

Purpose: This study was to examine the proliferation and function of the adipose tissue-derived endothelial cells according to different culture medium conditions. Materials and Methods: Adipose tissue-derived CD146 positive endothelial cells were cultured in according to different culture mediums (DMEM culture medium with or without osteogenic inductive agents and EBM-2 culture medium with or without osteogenic inductive agents). The proliferation and function of the adipose tissue-derived endothelial cells was examined in different culture medium conditions. Results: Adipose tissue-derived endothelial cells formed tube-like structures on Matrigel in EBM-2 culture medium with or without osteogenic inductive agents. However, the cells did not form tube-like structures on Matrigel in DMEM medium with or without osteogenic inductive agents. After 24 hours of culture, among the culture medium using EBM-2, the proliferation of the cells were promoted in EBM-2 medium without osteogenic inductive agents than in EBM-2 medium with osteogenic inductive agents. However, 72 hours of culture, the proliferation of the cells were promoted in EBM-2 medium with osteogenic inductive agents than in EBM-2 medium without osteogenic inductive agents. Conclusion: These results suggest that the proliferation and function of the adipose tissue-derived CD146 positive endothelial cells could be maintained in EBM-2 with osteogenic inductive agents.

북한의 국토 및 지역개발에 관한 고찰

김신원(Kim Shin Won) 한국전통조경학회 1997 한국전통조경학회지 Vol.15 No.2

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서울시 마을마당의 조성현황 및 개선방안 연구

김신원(Shin Won Kim),박율진(Yool Jin Park) 한국전통조경학회 1999 한국전통조경학회지 Vol.17 No.4

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한국의 전통조경 연구

김신원(Kim Shin Won),강현경(Kang Hyun Kyung) 한국전통조경학회 1995 한국전통조경학회지 Vol.13 No.2

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