Bacillus stearothermophilus DL-3을 사용하여 제조한 미생물 제재가 닭과 돼지의 성장에 미치는 영향

김순희,조강익,이유정,오주성,정순재,문병주,강경희,이진우,Kim Soon-Hee,Jo Kang-Ik,Lee You-Jung,Oh Ju-Sung,Jung Soon-Jae,Moon Byung-Ju,Kang Kyung-Hee,Lee Jin-Woo 한국생명과학회 2006 생명과학회지 Vol.16 No.1

유기물이 풍부한 토양에서 분리하여 동정한 Bucillus stearothermophilus DL-3균주의 배양액과 사과박, 간장박 및 미강 등을 사용하여 2종류의 미생물 제재를 제조하고 닭과 돼지의 생육에 미치는 영향을 조사하였다. 사과박의 주성분은 탄수화물이며 간장박의 주성분은 단백질이다. B. stearothermophilus DL-3 균주의 배양액과 미강을 사용하여 제조한 미생물 제제를 미생물 제제 A로 명명하고 B. stearothermophilus DL-3균주의 배양액, 사과박, 간장박 및 미강을 사용하여 제조한 미생물 제제를 미생물 제제 B로 명명하였다. 미생물 제재가 닭 및 돼지의 생육에 미치는 영향을 검토하기 위하여 일반사료를 급여한 구, 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 구 및 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 구로 나누어 효과 검정실험을 수행하였다. 실험에 사용한 각 구의 평균 병아리 무게는 각각 $41.1{\pm}2.5g,\;41.6{\pm}3.2g$ 및 $42.3{\pm}2.9g$이었다. 급여를 시작하지 28일 후, 각 구에 속한 병아리들의 평균 무게는 각각 $547.7{\pm}91.7g,\;560.1{\pm}17.2g$ 및 $562.2{\pm}32.5g$이었으며 각 구에 속한 병아리들의 평균 무게 증가는 각각 506.6 g, 518.5 g 및 519.9 g이었다. 즉, 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 군과 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 군은 일반사료를 급여한 군에 비하여 각각 $2.4\%$ 및 $2.6\%$의 무게 증가를 나타났다. 각 구의 평균 돼지 무게는 각각 $9.3{\pm}1.0kg,\;9.4{\pm}1.1kg$ 및 $9.6{\pm}1.0kg$이었다. 급여를 시작한지 35일 후, 각 구에 속한 돼지들의 평균 무게는 각각 $19.3{\pm}4.1kg,\;20.2{\pm}3.9kg$ 및 $20.8{\pm}4.2kg$이었으며 각 구에 속한 돼지들의 평균 무게 증가는 각각 10.7 kg, 10.8 kg 및 11.2 kg이었다. 닭의 사료 효과 검정 결과와 같이 일반사료의 $10\%$를 미생물 제재 A로 대체하여 급여한 군과 일반사료의 $10\%$를 미생물 제재 B로 대체하여 급여한 군은 일반사료를 급여한 군에 비하여 각각 $1.6\%$ 및 $5.2\%$의 무게 증가를 나타났다. Effect of microbial products made of Bacillus stearothermophilus DL-3 on growth of chickens and pigs was investigated. Two types of microbial product were made in this study. One is the microbial product made of culture broth of B. stearothemophilus DL-3 and rice bran which named as the microbial product A. The other is the microbial product made of culture broth of B. stearothermophilus DL-3, apple pomace, soybean pomace and rice bran which named as the microbial product B. Chickens were divided into three groups and each group was fed with $100\%$ general feed, $90\%$ general feed supplemented with $10\%$ microbial product A or $90\%$ general feed supplemented with $10\%$ microbial product B. The average chicken weight of each group was $41.1{\pm}2.5g,\;41.6{\pm}3.2g\;and\;42.3{\pm}2.9g$ and those after 28 days was $547.7{\pm}91.7g,\;560.1{\pm}17.2g\;and\;562.2{\pm}32.5g$, respectively. The average weight gain for each group was 506.6 g, 518.6 g and 519.9 g, respectively, and weight increases of groups fed with $90\%$ general feeder and $10\%$ microbial product A and B were $2.4\%\;and\;2.6\%$ higher than the group fed with $100\%$ general feed. Pigs were also divided into three groups and each group was fed like chickens. The average weight of each group was $9.3{\pm}1.0kg,\;9.4{\pm}1.1kg\;and\;9.6{\pm}1.0kg$ and those after 37 days was $19.3{\pm}4.1kg,\;20.2{\pm}3.9kg\;and\;20.8{\pm}4.2kg$, respectively. The average Weight gain for each group was 10.65 kg, 10.82 kg and 11.20 kg, respectively, and weight increases of groups fed with $90\%$ general feeder and $10\%$ microbial product A and B were $1.6\%$ and $5.2\%$ higher than the group fed with $100\%$ general feed.

임상실습경험에 따른 간호대학생의 죽음에 대한 태도와 자아존중감 및 삶의 만족도 비교

김순희,김동희,손현미,Kim, Soon-Hee,Kim, Dong-Hee,Son, Hyun-Mi 한국호스피스완화의료학회 2011 한국호스피스.완화의료학회지 Vol.14 No.3

목적: 본 연구는 간호대학생의 임상실습 경험유무에 따른 죽음에 대한 태도, 자아존중감 및 삶의 만족도의 차이를 파악하기 위한 서술적 조사연구이다. 방법: 양산시에 위치한 2개 대학에 재학 중인 간호대학생 1,030명으로 대상으로 하였다. 자료수집은 2010년 10월 1일~10월 30일까지 강의시간 이외의 시간에 연구의 목적과 방법을 설명하고 설문에 대한 응답은 익명으로 처리됨을 알려 준 뒤 연구에 참여하기로 자발적으로 동의한 대상자에게 서면동의서와 설문지를 배부하고 개별적으로 설문내용을 작성하도록 한 후 현지에서 직접 회수하였고, 수집된 자료는 PASW statistics data editor 통계 프로그램을 이용하여 실수, 백분율, 평균, 표준편차, 카이제곱 검정, t 검정, 분산분석, Scheffe 검정과 Pearson correlation coefficients로 분석하였다. 결과: 죽음에 대한 태도 점수는 임상실습 경험이 있는 간호대학생의 경우 2.85점, 실습경험이 없는 간호대학생의 경우 2.79점으로 중간수준이었으며, 임상실습 경험이 있는 간호대학생이 임상실습 경험이 없는 간호대학생에 비해 죽음에 대해 보다 긍정적인 태도를 가지고 있었고 자아존중감과 삶의 만족도가 높았다. 임상실습 경험이 있는 간호대학생의 죽음에 대한 태도는 자신이 지각하는 경제수준과 본인이 지각하는 자신의 건강상태에 따라 죽음에 대한 태도의 차이가 있었으며, 자아존중감과 만족도와 낮은 상관관계가 있었다. 결론: 간호제공자의 죽음에 대한 태도는 임종간호의 가장 중요한 요소로 간호의 질을 결정함을 감안할 때 미래의 간호사가 될 간호대학생의 죽음에 대한 태도를 보다 긍정적으로 변화시킬 필요가 있다. 특히 임상실습 경험이 없는 간호대학생이 죽음에 대해 보다 부정적인 태도를 가지고 있었고 자아존중감과 삶의 만족도가 낮았으므로 이들에게 보다 적극적인 관심이 필요할 것이다. 한편 임상실습 경험 유무에 따른 간호대학생의 죽음에 대한 태도, 자아존중감 및 삶의 만족도에 대한 선행연구가 부족하여 논의하는데 제한이 있으므로 반복연구가 필요할 것을 제언한다. Purpose: This study was conducted to compare nursing students' attitudes toward death, self-esteem and life satisfaction according to clinical experience. Methods: We surveyed 1,030 nursing students from two different universities with a questionnaire consisting of items regarding attitudes toward death, self-esteem and life satisfaction. The data were analyzed using a PASW program. Results: Students with clinical experience scored 2.85 on the attitude toward death, while those without clinical experience scored 2.79 (t=2.775, P=0.006). Students with clinical experience had significantly higher self-esteem (t=4.541, P<0.001) and life satisfaction (t=4.050, P<0.001) than those without it. Students with clinical experience showed correlations between attitude toward death and self-esteem (r=0.159, P<0.001) and life satisfaction (r=0.090, P=0.025), while those without it did not (r=0.106, P=0.014). Conclusion: These results provide guidance for developing educational strategies such as educational program to help students build positive attitude toward death through their experience in clinical practice.

노화된 흰쥐의 뇌줄기에 분포하는 NADPH-Diaphorase 및 Neuropeptide Y양성 신경세포에 관한 연구

김순희,장환일,허영범,안희경,Kim, Soon-Hee,Chang, Hwan-Il,Huh, Young-Buhm,Ahn, Hee-Kyung 대한생물정신의학회 1996 생물정신의학 Vol.3 No.2

This study was aimed to charily the change of NADPH-diaphorase(NADPH-d) and neuropeptide Y(NPY) associated with aging of the rots. To verily the effect of aging of NPY and NADPH-d-positive neurons in the brain stem, the neurons were stained by the immunohistochemical and histochemical method. In the aged group, the number of NADPH-d-positive neurons was significantly decreased in substantia nigra lateralis, supragenual nucleus, raphe magnus nucleus and raphe obscurus nucleus as compared with control group. The number of NADPH-d-positive neurons was not significantly decreased in superior colliculus, interpeduncular nucleus, central gray. dorsal raphe nucleus, retrorubral nucleus, pedunculopontine tegmental nucleus, laterodorsal tegmental nucleus, pontine reticular nucleus, prepositus hypoglossal nucleus and nucleus solitarius of the aged rats. The NADPH-d and NPY-positive neurons were found in the interpeduncular nucleus, central gray, substantia nigra lateralis, laterodorsal tegmental nucleus, nucleus solitarius, raphe magnus nucleus, raphe obscurus nucleus of the control and aged groups. The coexistence of NADPH-d and NPY in the some cell was not found in the brain stem of both groups.

인체에 적용한 전기자극이 Serum myoglobin과 Aldolase에 미치는 영향에 대한 생리학적 효과

김순희,천기영,최영덕,Kim, Soon-Hee,Chon, Ki-Young,Choi, Young-Deok 대한물리치료과학회 1999 대한물리치료과학회지 Vol.6 No.3

Prior studies have revealed that several stimulation to the muscle have released serum myoglobin into the blood vessel and increased aldolase activity. The present authors carried out a study which effect of electrical stimulation treatment (induced a isotonic wrist exerceise) on serum myoglobin(Mb) levels and aldolase(Al) activity were investigated in 6 healthy female. There were four groups of female: 1. no electrical stimulation control: 2. electrical stimulation 10min (EST10'); 3. electrical stimulation 20 min (EST20'); 4. electrical stimulation 30min (EST30'). Each groups is all the same one. Radioimmunoassay and Ultraviolet Spectrophotometry were performed to increased or decreased of serum myoglobin and aldolase. Serum myoglobin significantly increased in electrical stimulated groups[EST10' $(30.20{\pm}5.27ng/ml)$, EST20'$(31.65{\pm}3.96ng/ml)$, EST30'$(31.95{\pm}2.0ng/ml)$] to be compared with control group$(24.43{\pm}2.20ng/ml)$. Aldolase significantly increased in electrical stimulated groups [EST10' ($6.85{\pm}1.17$ Sigma U/mL), EST20'($6.70{\pm}1.46$ Sigma U/mL), EST30'($6.56{\pm}1.01$ Sigma U/mL)) to be compared with control group($5.03{\pm}1.86$ Sigma U/mL). The results of this study show that isotonic exercise result in electrical stimulation treatment increased serum myoglobin content and aldolase activity. In conclusion, our results support that stimulation release serum myoglobin and increase aldolase activity.

기록관리 전문가의 실무능력 제고를 위한 교육 프로그램 연구

김순희,Kim, Soon-Hee 한국도서관정보학회 2009 한국도서관정보학회지 Vol.40 No.3

기록관리 전문가는 업무의 특성에 따라 학문적지식과 실무능력을 갖추어야 한다. 특히 기록관은 정보기술의 발달에 따라 계속하여 변화하고 있어 폭넓은 실습을 통한 기술적인 전문성이 있는 실무능력을 갖춘 기록관리 전문가를 요구하고 있다. 그러나 현재 국내 기록관리 대학원들의 교육과정은 이론교육에 치중되어 있어 실무교육이 미흡한 실정이다. 따라서 본 논문은 미국과 한국의 대학원 기록관리 교육과정에서 실무능력 향상을 위한 요소의 현황을 비교 분석하여 한국 기록학 대학원의 교육과정을 이론적인 지식은 물론 실제적인 업무능력을 배양할 수 있는 방안을 제언하였다. 첫째, 한국의 기록학 교육과정 중 실무영역을 강화를 위해 전공학점의 및 이수학점의 증대, 실습을 필수 교과과정으로 할 것을 제언하였다. 둘째, 내실 있는 실습을 위한 관 학 산 협동의 인턴쉽 프로그램의 개발 및 실시를 제언하였다. 셋째, 현재 기록관리 업무에 종사하고 있는 기록관리 전문가들이 새로운 지식과 기술을 습득하여 그 전문성을 제고할 수 있는 계속교육프로그램을 실시할 것을 제언하였다. A Records manager and archivist should have the academic knowledge and working ability due to the working character. Archives and records centers are being changed with developing information technology so that they are requiring a records manager and archivist to have a professional working ability through a variety of practice. However, in recent, the curriculum of Korean archival graduate schools focuses on a theoretical field and neglects to apply a theory to a working field. Therefore, in this paper, the writer compares some elements to improve the working ability shown in the curriculum of Korean and American archival graduate programs, and suggests the method to cultivate a theoretical knowledge as well as working ability. The cultivating ways the writer suggests are as follows: To improve the working ability in a practical field of the curriculum of archival graduate school in Korea, the writer suggests -- 1. increasing the grades of major field and college courses, and changing the practice subject to an obligatory subject, and 2. developing an internship program for a full practice with company, government, and college, and 3. operating a continuous training program that an records manager engaging in records center and archives can acquire a new knowledge and technology and apply the professionalism to a working field.

간호대학생을 위한 죽음교육 프로그램 개발 및 효과

김순희(Kim, Soon-Hee),김동희(Kim, Dong-Hee) 기본간호학회 2015 기본간호학회지 Vol.22 No.3

Purpose: This study was done to develop a death education program for nursing students and evaluate the effects of the program. Methods: The education program was developed based on ADDIE model. The death education program was developed on the base of educational needs, a comprehensive review of the literature and focus group interviews and then evaluated with 53 nursing students, 27 in the experimental and 26 in the control group. Measurement was done for the meaning of life using the tool by Choi et al (2005) for attitudes concerning death, the tool Thorson and Powell (1998) revised by Kim (2006) and for attitude to end-of-life patient care, the Attitudes toward Nursing Care of the Dying Scale by Frommelt (1991) translated by Cho and Kim (2005). Results: The program consisted of five sessions: Understanding of death, Family bereavement care, Communication and End-of-life patient care, Professional role, and Ethics and legal issues There was a statistically significant difference between pretest and posttest for the meaning of life in the experimental group compared to the control group. About 82% of students in the experimental group were satisfied with the program. Conclusion: The results indicate that this program can be used to educate nursing students.

항공사 지상직원의 갈등, 갈등대처전략과 구성원 행동 간의 구조적 관계

김순희(Kim Soon Hee) 대한관광경영학회 2017 觀光硏究 Vol.32 No.1

본 연구는 항공사 지상직을 대상으로 조직 내 역할갈등 및 상사부하간갈등, 갈등대처전략 그리고 구성원의 행동(소진, 이직의도) 간의 구조적 관계를 파악하였다. 설문 대상은 아시아나항공, 대한항공, LCC으로 부산 김해국내/국제공항과 서울 김포공항, 인천 국제공항 내에서 주로 예약발권, 탑승수속, 출도착, 카운터 등의 업무를 담당하는 지상직원이고, 2016년 8월 20일~2016년 10월 16일 동안 280부의 설문이 진행되었고 그 중 회수되지 않거나 응답이 불성실한 설문지를 제외하고 총 227부를 최종분석에 이용하였다. 수집된 자료는 SPSS 18을 이용하여 신뢰도 분석과 상관관계를 이용하였고, AMOS 18을 이용하여 확인적 요인분석, 측정모델 분석 및 제안모델 분석 등을 검정하였고, 추가로 대안모델 개발을 하였다. 분석을 위해 SPSS 18과 AMOS 18을 이용하였고, 신뢰도 분석을 위해 Crombach s Alpha와 변수 간의 인과관계 파악을 위해 측정변수모델(SEM)을 이용하였으며, 추가분석으로 갈등(역할갈등, 상사부하간갈등)과 갈등대처전략 간의 관계에서 소진현상의 매개효과를 검정하고, 갈등대처전략의 모델 차이검정을 실시하였다. 본 연구에서 설정된 가설은 총 8개로 그 중 5개의 가설 1, 3, 5, 6, 7은 의미 있는 가설로 채택되었고, 나머지 2개의 가설 2, 4는 기각되었으며 가설 8은 의미 있는 결과가 나왔다. 연구결과를 기초로 결론과 시사점, 그리고 본 연구의 한계점과 향후 연구의 방향을 제시하였다. The purpose of this study was to determine the structural relationship between Conflict(Role Conflict, Leader-Member Conflict), Conflict Coping Strategies and behaviors of members (Burnout, Turnover Intention) with airline ground staffs. Surveyed airline ground staffs are responsible for tasks such as Asiana Airlines, Korean Air, LCC Busan Gimhae Domestic / International Airport and Gimpo Airport in Seoul, mainly reservation ticketing, check-in, arrival and departure from the Incheon International Airport, counter. August 20~October 16, 2016 and among questionnaires distributed and collected, significant 227 were employed in this study The data collected was used for reliability analysis and correlation using SPSS 18, Black was a confirmatory factor analysis, analysis of measurement model, the proposed model analysis using AMOS 18. And it was developed as an additional alternative models.For analysis, SPSS 18 and AMOS 18 were used, and for reliability analysis, measurement variable model (SEM) was employed in order to understand a casual relationship between Crombach s Alpha and variables, and alternative model development was made for additional analysis. Hypotheses were 8 in total and among them, hypotheses 1,3,5,6 and 7were adopted for significant ones, and the rest hypotheses 2, 4 were rejected. Hypothesis 8 suggests that meaningful results. Based on study findings, conclusions, implications, limits of the present study and direction of the future study were proposed.

Effect on Apple pomace and Soybean pomace on cell growth of Bacillus stearothermophilus DL-3

Soon-Hee Kim(김순희),Kae-Sun Bae(배계선),Ji-Mo Kim(김지모),Hyung-Pil Seo(서형필),Ju-Sung Oh(오주성),Soon-Jae Jung(정순재),Byung-Ju Moon(문병주),Jin-Woo Lee(이진우) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4

연구논문 : 연령기에 따른 여성모발의 특성 및 아미노산 조성에 관한 연구

김순희 ( Soon Hee Kim ) 한국미용학회 2007 한국미용학회지 Vol.13 No.3

In this paper, we have studied on the ratio and content of amino acid in the sample virgin hairs which has no beauty treatment for total 60 person for each 20 for 8 year old girls, 20~30 years old and over 65 years old women. As the result, we found there are difference in the amino acid among the hairs of elderly Women, girl and adult women except Serine, Glycine, Tyrosine, Methionine, Tryptophan. Namely, it is shown that content of amino acid for elderly women is lower than that for girl and adult women. But, there is not show difference between the content of amino acid for girl and adult women. Content of amino acid for girl`s hair has almost same quantity of content of adult women`s hair. here is not show difference statistically in the ratio of content of amino acid among the girls, adult women, elderly women. It is shown that the ratios of content of amino acid among the each sample was almost regular. Content of 6 types amino acid such as Cyx*, Glx***, Proline, Serine, Leucine, Glycine occupied over 50%, it shows that it takes higher ratio relatively. Others, the content of amino acids such as Tryptophan, Methionine, Histidine, Phenylalanine are shown that these are included in very small quantity.

간호대학생의 죽음에 대한 태도, 죽음관련 교육경험과 교육 요구도

김순희(Kim, Soon Hee),김동희(Kim, Dong-Hee) 한국간호교육학회 2011 한국간호교육학회지 Vol.17 No.3

Purpose: This study was to investigate nursing students attitude to death, death-related education experience and educational needs. Methods: The participants of this study were 1,134 nursing students studying in three universities in Gyeonsangnam-do. Data collection was carried out from October 1st to November 31st, 2010. The collected data was analyzed for descriptive statistics, t-test, ANOVA and Sheffe test using PASW Statistics 18.0. Results: The level of attitude to death of the subjects averaged 2.60. The item receiving the lowest score was ‘I am not afraid of a long, slow death. Approximately 62.0% of the nursing students had no death-related education experience and 89.8% of those answered that death-related education was necessary. For attitude to death followed by general characteristics, death-related education experience and educational needs, there were significant differences in attitude to death according to gender, religion, perceived health status, worrying about problems, motivation of nursing, and an experience with death. Conclusion: As nursing students have a high educational need university curriculum development and educational programs addressing death should be developed and applied to nursing students.