Oxidant에 의한 신장세뇨관 물질이동계의 장애에 대한 단삼(丹參)의 효과

김상범,정지천,Kim, Sang-Beum,Jeong, Ji-Cheon 대한한방내과학회 1997 大韓韓方內科學會誌 Vol.18 No.1

This study was undertaken to determine whether Salviae-radix (SVR) exraction exerts benefical effect against oxidant-induced inhibition of tetraethylammonium (TEA) uptake which is actively secreted by renal proximal tubules. TEA uptake increased as function of incubation time to 60 min. When renal cortical slices were exposed to 50 mM $H_2O_2$, TEA uptake was significantly inhibited. The inhibition was significantly protected by addition of 0.5% SVR extraction. The benefical effect of SVR was dose-dependent over the concentration range of 0.1-1%; $H_2O_2$ (50 mM)-induced inhibition of TEA uptake was completely protected by 0.5-1% SVR extraction. $H_2O_2$ increased LDH release which was accompanied by an increase in lipid peroxidation in renal cortical slices. These changes were prevented by 0.5% SVR. These results suggest that SVR exerts benefical effect against oxidant-induced impairment of membrane transport function, this effect may be due to by an antioxidant action.

H₂O₂에 의한 腎臟 세포 손상에 대한 丹蔘 추출물의 방지 효과

金尙範(Sang-beum Kim),鄭智天(Ji-cheon Jeong) 대한한의학회 1998 대한한의학회지 Vol.19 No.1

패혈증 환자에서 Ranitidine의 투여가 T-림프구 및 그 아형에 미치는 영향

조영현(Young Hyun Cho),하우송(Woo Song Ha),박순태(Soon Tae Park),홍순찬(Soon Chan Hong),한호성(Ho Seong Han),제정인(Jung In Je),김상범(Sang Beum Kim),김상범(Sang Beum Kim),이옥재(Ok Jae Lee) 대한외과학회 1993 Annals of Surgical Treatment and Research(ASRT) Vol.45 No.5

제주(濟州) 산천단(山川壇)의 신원(新苑),성림적(聖林的) 가치(價値)와 의미(意味)

노재현 ( Jae Hyun Rho ),신상섭 ( Sang Sup Shin ),이상훈 ( Sang Hoon Lee ),김상범 ( Sang Beum Kim ) 韓國傳統造景學會 2009 한국전통조경학회지 Vol.27 No.4

요추에 있어서의 후관절각

이규열(Kyu Yeol Lee),손성근(Sung Keun Sohn),김상범(Sang Beum Kim),이형환(Hyung Hwan Lee) 대한정형외과학회 1997 대한정형외과학회지 Vol.32 No.2

조선선비가 설정한 구곡원림의 현황과 경물 특성

노재현 ( Rho Jae-hyun ),최영현 ( Choi Yung-hyun ),김상범 ( Kim Sang-beum ) 한국전통조경학회(구 한국정원학회) 2018 한국전통조경학회지 Vol.36 No.3

조선시대 설정된 구곡의 현황과 그 실태를 파악하기 위한 기초적 단계로서 남한 지역에 분포하는 구곡원림의 분포현황과 설정된 구곡의 경물 특성을 파악한 본 연구의 결론은 다음과 같다. 첫째, 국내에 분포하는 구곡원림은 대경권에 55개소(51.4%), 충청북도에 22개소(20.56%)가 집중 분포하는 것으로 확인되었다. 구곡이 집중 분포된 곳은 소백산-월악산-속리산을 잇는 백두대간상의 좌우안과 낙동정맥 우안의 낙동강 유역에 집중되는 경향이 매우 강하게 나타났는데, 이는 영남 성리학파의 학풍과 그들의 원림 향유와 밀접하게 관련된 것으로 판단된다. 둘째, 기초지자체별로 구곡의 분포를 살펴 본 결과, ‘한국 정신문화의 수도’로 자타가 공인하는 안동시에 10개소(9.35%)로 가장 많은 구곡원림이 존재하는 것으로 확인되었고, 다음으로 괴산군 9개소(8.41%), 문경시 8개소(7.48%), 봉화군 6개소(5.61%), 영주시 5개소(4.67%) 등의 순으로 나타났다. 셋째, 구곡원림의 설정 시기는 18C 33개소(33.0%)를 필두로, 19C 33개소(33.0%), 20C 14개소, 17C 13개소, 16C년 4개소 등의 순으로 16세기 이후부터 조선 말기까지 꾸준히 구곡문화가 전파된 것으로 파악되었다. 특히 조선 후기인 18~19C(66.0%) 사이에 높은 빈도로 구곡원림이 설정된 것으로 나타났다. 넷째, 곡의 집경형태는 ‘구곡’이 97개소(90.65%)로 압도적이었으며 그 중 상향식 구곡은 총 99개소(92.5%), 하향식 구곡은 총 8개소(7.5%) 구곡으로 확인되었다. 다섯째, 구곡 콘텐츠 중 구곡시는 67개소(64.49%)로 가장 많이 나타났고 바위 글씨가 29개소(27.10%), 구곡도는 16개소(14.95%)가 존재하는 것으로 확인되었다. 여섯째, 구곡 경관구성에 가장 많이 등장한 경물은 대(臺)가 124개(13.05%)로 월등히 많았는바, 이는 조선구곡의 특성을 부각하는 경물 속성으로 볼 수 있다. 이어 암(岩)을 포함한 암(巖)이 115개(11.2%), 담(潭) 73개(7.68%), 정(亭) 48개(5.05%), 동(洞) 39개(4.10%), 산(山) 36개(3.78%), 봉(峯) 27개(2.84%), 연(淵) 23개(2.42%), 천(川)·탄(灘) 각각 22개(2.31%) 등의 순으로 나타났다. 향후 구곡의 총 연장거리 및 경사도 등 지형 등을 감안한 구곡의 입지유형 분류 등의 연구가 뒤따라야 진정한 구곡 원림의 경물 특성 및 입지분포 특성이 더욱 명료하게 부각될 것으로 예상된다. This study was attempted to understand present status and characteristics of landscape components of the domestic Korean Gugokwonlim created by the classical scholars in the Joseon Dynasty. The results are as follows. First, Distribution of Gukokwonlim in Korea shows that 55(51.4%) are located in Daegu and Gyeongsangbuk-do, and 22(20.56%) are located in Chungcheongbuk-do. Concentrated locations of Gugok are on the part of Baekdudaegan, from Sobaeksan mountain to Sokrisan mountain via Wolaksan mountain, and the Nakdong River basin of the Nakdong vein in the right bank. This consideration seems to be closely related to the academy of Yeongnam Confucianism and the their trend of Wonrim enjoyment. Second, according to the result of examining the distribution of Gugok according to the basic local government authority, The biggest number of the Gugok places(10 places, 9.35%) are located in Andong, which is called 'the capital of Korean spiritual culture.' Additionally in order, 9 places(9.45%) is located in Goesan, 8 places(7.48%) in Mungyung, 6 places(5.61%) in Bonghwa, and 5 places(4.67%) in Yeongju. Third, in order to the creating time of Gugokwonlim, 33 (33.0%) were created in 18^th century, and other 33 (33.0%) were created in 19^th century. In addition, 14 were created during 20^th century, while 13 were created in 17^th century. And 4 were created in 16^th century. Respectively. great number of 18^th and 19^th centuries shows that many(66.0%) Gugokwonrim were created between late 18th to 19th centuries. Fourth, There were 97(90.65%) of 'Gugok' in the form of collecting type, and a total number of bottom-up style Gugok were 99(92.5%) while top-down style Gugok were 8(7.5%). Fifth, Among the contents of Gugok, 67 were found in pome of Gugok(64.49%), 29 caved letters in rock(27.10%), and 16 in painting of Gugok(14.95%). Sixth, The most emerged landscape components of Gugok was Dae(臺) 124(13.05%), followed by Am(巖) 115[11.2%, including of Am(岩)] 115(11.2%), and Dam(潭) 73(7.68%), Jeong(亭) 48(5.05%), Dong(洞) 39(4.10%), San(山) 36(3.78%), Am(岩, rocks) 31(3.26 %), Bong(峯, peaks) 27(2.84%), Yeon(淵) 23(2.42%) and Chun(川) and Tan(灘) 22(2.31%). Mostly, common landscape components of Gugok are entrusted natural things. It is expected that more studies about the analysis of characteristics of Gugok’s positioning types considering total distance and a gradient are required to understand more clearly characteristics and location distribution of true Gugok and its landscape components.

폐암 환자와 폐암 세포주를 주입한 Nude Mice에서 종양성장인자의 변화

김상범,박성달,김송명,허방 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

Background and purpose : The incidence of non-small cell lung cancer has decreased than past decades but the prevalence rate of squamous cell carcinoma is highest among the various types of lung cancer. There have been one of the most difficult problems of the medical areas because of the law rate of operative treatment by micrometastasis still. The released growth regulating factors from the tumor cells have heen researching targets recently. This study is to know the changes of the tumor growth factors by examining the change of transforming growth factor-β1 (TGF-β1) within plasma of nude mouse was measured after each of different quantity of cultured cell of squamous cell carcinoma was injected into plerual caviry of nude mouse in animal experiment, while comparing the values of TGF, Insulin-like growth factor and epidermal growth factor between experimental trial and clinical trial. Method : Between June of 2006 and June of 2007 at Kosin University Hospital, 17 patients were chosen for this study where 13 of them were pathologically diagnosed of squamous cell carcinoma after initial diagnosis of lung cancer followed by radical pneumonectomy and pathologic biopsy, while 4 patients were pathologically diagnosed of benign tumor. Blood sample was collected prior to a surgery from the lung cancer patients scheduled for surgery, and the sample was cryopreserved. During surgery, dense tumor tissue without necrosis was excised in a size of 5mm3 and quickly frozen to be used for experimental tissue, whereas the tissue, which was located farthest from the lesion and deemed free of cancer cell, was to be used for control tissue. And the tissue obtained from benign tumor or normal tissue of granuloma patient was classified as a control tissue B, while the sample from malignant tissue of lung cancer was categorized as experimental tissue M. In animal experiment with 15 male nude mice without immunity, 5 subjects without cancer cell were used for control group, another 5 subjects with of 2×106 of cancer cell line (SW-900 G IV) injected into intrathoracic were used for experimental group I, and the other 5 subjects injected with twice quantity of cancer cell line of group I were classified as experimental group II. After breeding the subjects in the clean room for 8 weeks, blood sample was collected from their heart, and quantitative test of plasma TGF-β was performed. For the test of tumor growth factor, human TGF-β ELISA kit was used for quantitation of TGF-β1&2, and active TM non-eztraction IGF kit was for quantitation of IGF-I&II, while GE healthcare kit was used for EGF. Results : In animal experiment, TGF-β1 was expressed within the serum of all nude mice from control group as well experimental group I and II, where control group was at 28.49 fmol/ml while experimental group I and II were at 32.19 fmol/ml and 42.36 fmol/ml respectively. In clinical trial, TGF-β1 was measured higher from experimental group with 40.9 fmol/ml than from control group with 28.5 fmol/ml, and TGF-β2 was lower from experimental group with 12.3 fmol/ml than from control group with 30.3 fmol/ml, while the difference was insignificant with EGF (p<0.05) where control group and experimental group were at 0.11 ng.ml and 0.12 ng/ml respectively. Moreover, TGF-β1 was at 40.88 fmol/ml from control tissue B and at 15.55 fmol/ml from experimental tissue M, where as TGF-β2 was at 12.31 fmol/ml from control tissue B and 23.95 fmol/ml from experimental tissue M (p<0.05). TGF-β1 within the serum of control group was at 812.4 fmol/ml before surgery and at 989.1 fmol/ml after surgery, which showed a slight amplifiction, while TGF-β2 was at 16.8 fmol/ml before surgery and at 20.2 fmol/ml after surgery, exhibiting a slight increase (p<0.05). Serum IGF-I was at 117.9 ng/ml before surgery and at 214.4 ng/ml after surgery, and IGF-II was at 552.8 ng/ml and 552.8 ng/ml after surgery, while EGF showed insignificant changes between before surgery at 0.67 ng/ml and after surgery at 0.74 ng/ml (p<0.05). In lung cancer stage Ia,b, TGF-β1 was decreased through surgery as it was at 972.94 mol/ml before and 866.92 mol/ml after, and TGF-β2 was also decreased as measured at 42.40 mol/ml before surgery and 38.24 mol/ml after surgery. In stage IIIa,b, TGF-β1 was decreased as measured at 980.31 mol/ml before surgery and at 928.18 mol/ml, while TGF-β2 showed not much changes (p<0.05). In lung cancer stage Ia,b, serum IGF-I was decreased as measured at 112.7 mol/ml before surgery and at 87.92 mol/ml after surgery. and IGF-II was also decreased as measured 468.78 ng/ml before surgery and at 358.59 ng/ml after surgery (p<0.05). In stage III,b, IGF-I showed not much changes, but IGF-II was at 438.31 ng/ml before surgery and 404.2 ng/ml after surgery, where EGF was not all influenced by the phase. Conclusion : In the experiment with nude mice, TGF-β1 was increased as the number of cancer cell was increased after injection of cancer cell. The number of cancer cell from early tumor, such as Ia,b from post-surgery clinical lung cancer patients, was reduced after the surgery, and TGF-β1 was also reduced as the cancer cell was reduced as well. Therefore, it was believed that TGF-β1 can be a feasible prognostic factor for early diagnosis of lung cancer at Ia,b of early phase

포-만트 VOCODER의 구성에 관한 연구

심장엽,안종영,김상범,허강인 東亞大學校 附設 情報通信硏究所 1995 情報通信硏究所論文誌 Vol.3 No.1

본 논문에서는 포-만트 vocoder를 구성하여 한국어 음성 합성을 연구하였다. 포-만트 vocoder의 구성에 필요한 합성파라메터로서는 스펙트럼 모멘트법에 의한 포-만트 주파수, 최적 Comb법에 의한 피치 주파수, 단시간 평균에너지와 단시간 평균진폭, 대역폭, 음원파형, 가우시안 백색잡음이다. 합성실험은 남성 화자가 발성한 음절, 단어, 문장 등의 음성데이터를 이용하였다. 청취결과, 유무성음에 대해 각각 Rosenberg의 음원파형과 삼각파를 음원으로 적용한 경우가 우수하였다. This paper studies on a method of Korean speech synthesis by composing formant VOCODER. The parameters of speech synthesis are formant frequencies by spectrum moment method, pitch frequencies by optimum Comb method, short time average energy, short time average amplitude, bandwidth, excitation wave and gaussian white noise. In this paper, synthesis is performed using speech data which are syllable, word and sentence spoken by man. Synthesis results are good in case of using Rosenberg & Triangle wave respectively as voice source.

중간 저장조 운영방안을 고려한 비순차 다목적 회분식 공정의 최적조업순서 결정

이호경,김상범,이인범,이의수 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.1

이 논문에서는 다양한 중간 저장조 운영 방안에 따른 비순차 다목적 회분식 공정의 최적 조업 순서 결정을 위한 수학적 모델을 제안한다. 비순차 다목적 회분식 공정에서는 다품종 공정의 경우와 달리 각제품의 제조 경로가 서로 다를 뿐 아니라 역방향 제조 흐름이 존재한다. 따라서, 장치 유휴시간을 줄여 공정의 효율을 높이려면 각 제품의 제조 경로와 중간 저장조 운영 방안을 고려해 각 장치에서 제품 처리 순서를 서로 다르게 결정해야만 한다. 이를 위한 접근 방법으로 주어진 중간 저장조 운영 방안에 대해 각 장치에서 이루어지는 조업의 시작과 완료 시간을 제품과 처리순서 기준으로 각각 표현한 후 이진 변수와 논리적 제약 조건을 이용해 두 표현에 사용된 변수를 일치시켰다. 모델은 혼합정수선형계획법(Mixed Integer Linear Programing, MILP) 형태로 표현되었으며, 세 가지 예제에 적용하여 제안된 모델의 효용성을 보였다. In this paper, we present mathematical models for optimal scheduling of non-sequential multipurpose batch processes under various intermediate storage policies. Compared with multiproduct processes, in non-sequential multipurpose batch processes, the production routes of products may be different from one another and may be backward direction. Consequently, in order to reduce idle time of units and to raise the efficiency of process, we have to make operation sequences of products in each unit differently by considering processing route of each product with a given intermediate storage policy. The basic concept of our approach for these problems is as follows. Firstly, we represented the staging and finishing time of a task in each unit with two coordinates for a given storage policy. One is based on products, and the other is based on sequences. Then, we matched the variables used in the two coordinates into one with binary variables and logical constraints. We formulated these problems as MILP(Mixed Integer Linear Programming) models and apply them to three examples to show the effectiveness of the model.