다약제 내성 유전자를 이용한 항류마티스 약제의 내성 검사 방법 개발

김상경 ( Sang Gyung Kim ),서헌석 ( Hun Suk Suh ),최정윤 ( Jung Yoon Choe ),이종원 ( Jong Won Lee ),서장수 ( Jang Soo Suh ),김신규 ( Think You Kim ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.1

Objective: A number of disease-modifying anti-rheumatic drugs (DMARDs) have been shown to be more effective than placebo in the management of rheumatoid arthritis (RA). However, most course of DMARDs, except methotrexate, are discontinued after 2 or 3 years, because of toxicity, lack of efficacy or escape from control. The multi-drug resistance (MDR) is a phenomenon in which cells develop cross-resistance to many agents such as anthracyclin, vinca alkaloids and colchicine. In our hypothesis, MDR phenomenon could be implicated in acquired resistance to DMARDs in RA. We have established a mdr1 cell line and tested whether DMARDs are substrate for P-glycoprotein (P-gp). Methods: The mdr1-cDNA was cloned into retroviral vector, and the recombinant retroviral vector was transfected into PA317 cells. The target cells, NIH3T3, were infected with recombinant retroviruses. A colony most resistant to vinblastin was selected for the following experiments; expression of mdr1 gene in NIH3T3 cells was confirmed by RT-PCR, and biological function of mdr1 gene product, P-gp, was tested using Rhodamine-123 (Rh123) efflux assay. Resistance of the target cells expression P-gp which can survive against hydroxychloroquine (HCQ) and methotrxate (MTX) were measured by MTT assay. Results: RT-PCR for mdr1 gene showed successful transfer of the gene into the NIH3T3 cells. Rh123 assay revealed expression of P-gp on the selected cells as follows; Rh123 efflux activity of uninfected NIH3T3 cells was 6%, that of PLXSN was 0.2%, and that of selected cells was 44%. The 50% proliferation inhibitory capacity of the selected cells were twice for HCQ but there was no difference of that for MTX. Conclusion: We established a mdr1 cell line and using the cell line, HCQ was a substrate of MDR, but MTX was not related to MDR.

사람골수줄기세포가 연골조직으로 분화되는 과정에 나타나는 세포표지자의 표현

김상경 ( Sang Gyung Kim ),최정윤 ( Jung Yoon Choe ),김채기 ( Chae Gi Kim ),정승혜 ( Seung Hie Chung ),신임희 ( Im Hee Shin ),서헌석 ( Hun Suk Suh ) 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.1

Objective: Multipotent bone marrow stromal cells have the ability to differentiate toward a variety of connective tissue lineages including cartilage. The future use of adult mesenchymal stem cells (MSCs) for human therapies depends on the establishment of preclinical studies. Therefore, in this preclinical study we demonstrated the expression of MSC surface markers CD29, CD105, and CD44 on human bone marrow derived stromal cells during chondrogenic differentiation. Methods: Adult human bone marrow was collected from the iliac crest of 7 donors following informed consent. Mononuclear cells were isolated, incubated in monolayers, and embedded in alginate beads for three-dimensional cultures. Cellualr viability was assessed by MTT assay. Flow cytometry of alginate bead cultures was performed on days 0, 7, 14, 21, and 28 using monoclonal antibody against surface molecules, CD105, CD29, CD44, CD34 and CD45. Total contents of collagen and glycosaminoglycan (GAG) of the alginate beads was measured. SPSS 11.0 was used for data analysis. Results: After 7 days of culture, 89% of the cells expressed the human integrin beta 1 antibody, CD29. The CD29-positive cells remained elevated at 83% on days 28. However, while only 18% expressed the type II TGF-beta receptor endoglin, CD105 on day 7, the CD105-positive cells increased abruptly 65% on day 14 remaining elevated up to day 28. The expression of CD44 was maximal in the first passage cell (63%). High concentration of TGF-beta 3 (10 ng/mL) was more favorable for sustaining cell viability than a low concentration (0.5 ng/mL)(n=4, p=0.002, day 21). The total contents of collagen and GAG in the MSC-alginate beads increased during the three-dimensional culture (n=4, p=0.02, p=0.006) suggesting its differentiation into a chondrogenic lineage. Conclusion: CD29 was expressed earlier than CD105 during chondrogenic differentiation of human bone marrow MSC. CD44 expression was highest in the first passage cells and gradually decreased afterwards.

Retrovirus를 이용하여 조혈모세포에 유전자를 전달하기 위한 최적화

김상경(Sang-Gyung Kim),서헌석(Hun-Suk Suh),이종원(Jongwon Lee),신동건(Dong-Gun Shin),이재관(Jaegwan Lee),김현민(Hyun-Min Kim),김재식(jay-Sik Kim),서장수(Jang-Soo Suh) 한국생물공학회 1999 KSBB Journal Vol.14 No.5

식혈증후군이 동반된 성인형 스틸병 2예

김현숙 ( Hyun Sook Kim ),박근우 ( Keun Woo Park ),김수경 ( Soo Kyoung Kim ),김지영 ( Ji Young Kim ),박성훈 ( Sung Hoon Park ),신진향 ( Jin Hyang Shin ),김상경 ( Sang Gyung Kim ),최정윤 ( Jung Yoon Choe ) 대한류마티스학회 2007 대한류마티스학회지 Vol.14 No.2

Adult onset Still`s disease is an rare inflammatory disease with the characteristic of fever, skin rash, arthralgia or arthritis, lymphadenopathy, leukocytosis and multiple systemic organ involvement. Its accurate pathogenesis has not been elucidated yet. Its clinical manifestation is also very diverse, from relatively mild symptoms to severe complications such as concomitant infection, liver failure, disseminated intravascular coagulation, myocarditis, adult respiratory distress syndrome, which may lead to death in some cases. Particularly, concomitant hemophagocytic syndrome is rare complication that could induce a fatal outcome. Thus it is important to diagnose early and start treatments. Until now, it has been reported in only one case of adult onset Still`s disease in Korea. Here, we report two female cases of adult onset Still`s disease with concomitant hemophagocytic syndrome.

잦은 천명 증상의 재발과 혈장 내 VEGF 및 Plasmin 조절 인자들과의 관련성

김소연 ( So Yeon Kim ),권상미 ( Sang Mi Kwon ),박혜진 ( Hye Jin Park ),김우택 ( Woo Taek Kim ),김진경 ( Jin Kyung Kim ),최은진 ( Eun Jin Choi ),이계향 ( Kye Hyang Lee ),정혜리 ( Hai Lee Chung ),김상경 ( Sang Gyung Kim ) 대한소아알레르기 및 호흡기학회 2007 소아알레르기 및 호흡기학회지 Vol.17 No.4

웹기반 임상데이터 관리 시스템 구축을 위한 프로그램 개발

신임희,김달호,김상경,손기철,박전우,곽상규,Shin, Im-Hee,Kim, Dal-Ho,Kim, Sang-Gyung,Sohn, Ki-Cheul,Park, Chun-Woo,Kwak, Sang-Gyu 한국데이터정보과학회 2012 한국데이터정보과학회지 Vol.23 No.1

컴퓨터의 발달로 인해 여러 가지 현상들이 수치화되어 데이터로 수집되고 있다. 특히 많은 양의 데이터가 각 분야별로 수집되고 있는데 이는 데이터를 기초로 한 분석과 해석을 통하여 의사 결정의 뒷받침이 되는 정보를 얻기 위해서이다. 데이터의 중요성이 부각되면서 데이터의 관리가 관심사가 되었다. 많은 연구자들이 공유할 수 있도록 서버에 데이터베이스를 구축하고 이를 웹 브라우저를 통하여 조회 및 확인하는 시스템을 구축하는 것이 필요하다. 본 연구에서는 연구자가 가장 많이 사용하는 엑셀 파일을 서버 데이터베이스에 업로드 하였고, 웹기반의 데이터베이스와 연동하여 연구자가 업로드한 데이터를 조회 및 확인할 수 있는 웹기반 프로그램을 개발하였다. 웹을 기반으로 데이터를 업로드 할 DB로 오라클을 사용하였으며 데이터베이스를 조회하기 위하여 웹프로그래밍 언어인 html, JAVA, JSP 등을 사용하여 웹기반 임상데이터 관리 시스템 구축을 위한 프로그램을 개발하였다. Various phenomenon can be expressed numerically and collected as a data due to rapid development of the computer. In particular large set of data is collected in various fields. We can obtain the information for final decision based on analysis and interpretation of the data. The issue is the management of the data as well as the importance of the data. So a system which stores the data in server and prints out the data to web browser is demanded. We uploaded the file of Excel form to server database and developed a web based program which can show the uploaded data through web based database. We used the Oracle DB for uploading and web programming language such as html, JAVA, JSP for querying the data. Finally, we developed a program for web based clinical data management system construction.

통합의료적 황달진단법개발을 위한 통계적 접근방법

신임희,곽상규,김상경,손기철,정현정,조윤정,이아진,권오승,Shin, Im Hee,Kwak, Sang Gyu,Kim, Sang Gyung,Sohn, Ki Cheul,Jung, Hyun-Jung,Cho, Yoon-Jeong,Lee, A-Jin,Kwon, O Sung 한국데이터정보과학회 2013 한국데이터정보과학회지 Vol.24 No.3

Healthcare approach in Western medicine and Korean Traditional Medicine (KTM) varies from its nature of human understanding and cultural differences. This fundamental difference in their approach of the human pathology has dualised and hindered common medical communication between the two fields of medicines. Within this current difficulty, the integrative medical service is said to become a novel method to provide the patients with the best medical care as their intent is to adapt and combine the advantages stated from the two different fields. This research paper shows the integrative approach of treating jaundice, where the symptoms of dampness and heat on Korean traditional standards are analyzed using statistical methods based on monitoring the blood test results. Therefore, we can explore an approach to diagnose and treat with comprehensive and integrative medicine algorithm. 건강 관리에 있어서 서양의학과 한의학의 접근 방법의 차이는 자연과 인간의 이해에 대한 문화적인 차이에서 비롯된다. 서양의학에서는 자연과 인간을 분리하고 인간 또한 여러 하위 시스템으로 나누고, 질병을 외부의 자극에 대한 반응이 적절하지 못하여 초래되는 것으로 보고 질병이 발생되면 시스템 별로 진단하여 치료해 왔다. 반면, 한의학의 경우 자연과 인간을 하나로 보고 인간의 건강을 자연과의 조화로운 상태로 규명하고 질병이 발생하기 전에 건강의 균형을 유지하기 위한 면역 기능을 높이는 예방적 치료를 주로 해왔다. 이러한 인간에 대한 근본적인 접근방법의 차이는 의료 전달체계를 양분화 시키고 상호 의사소통의 어려움을 야기했으나 통합 의료 서비스는 두 가지 의학의 장점을 살리고 최상의 치료 효과를 지향하는 시도라고 할 수 있다. 따라서 본 논문에서는 특정 질환인 황달에 대해 한의학적 분류 (습증, 열증)에 따른 서양의학에서 사용하는 혈액학적 검사수치를 통계적 분석기법을 사용하여 살펴보고 차이가 있는 수치를 살펴봄으로써 통합의료적 환자 진단과 치료에 적용할 수 있는 접근 방법을 살펴보고자 한다.

천연 알긴산을 지지체로 한 삼차원 배양에서 연골세포의 생존력

류유정 ( You Jeong Lyou ),김상경 ( Sang Gyung Kim ),최연희 ( Yeon Hee Choi ),최정윤 ( Jung Yoon Choe ),김채기 ( Chae Gi Kim ),김종기 ( Jong Ki Kim ),윤연희 ( Yeon Hee Yoon ),신임희 ( Im Hee Shin ),박상옥 ( Sang Ock Park ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.3

Objective: Articular cartilage has a highly limited capacity to repair because of lack of blood supply. There have been no effective modality to regenerate the articular cartilage and prevent degenerative changes. It is necessary to proliferate the cells in vitro, however the cells lose their phenotype during in vitro monolayer culture. Although it is not enough to increase the number of the cells in the three dimensional culture, it is a effective way to maintain their original phenotype expression. Alginate has been used as a good source of scaffold in chondrocyte three dimensional culture. The objective of this study was to find the most favorable scaffold for chondrocyte viability among various alginate extracted from natural source in chondrocyte three dimensional culture. Methods: The alginate extracted from brown seaweed, Undaria pinnatifida and sea tangle, Laminaria japonica inhabitating near Korean sea and commercially available alginate were used. Chondrocytes isolated from adult pig were used. Three kinds of chondrocyte-alginate bead were made and incubated for forty-four days. Cellular viability and glycosaminoglycan (GAG) content were measured and compared. SPSS Version 10.0 was used for data analysis. Results: Viability of chondrocyte and GAG content were increased as a function of time. Alginate from brown seaweed, U. pinnatifida appeared more favorable to maintain chondrocyte viability than others. The total GAG content was similar among three kinds of alginate Conclusion: Alginate extracted from natural see weed, especially brown seaweed, may be a good source to maintain chondrocyte viability in three dimensional culture.

류마티스 관절염의 관절액 Total Fibronectin의 임상적 의의

김학준 ( Hak Jun Kim ),박정기 ( Jeong Ki Park ),여동근 ( Dong Geun Yoe ),윤원찬 ( Wern Chan Yoon ),정의달 ( Ye Dal Jung ),조선주 ( Sun Joo Cho ),김상경 ( Sang Gyung Kim ),전창호 ( Chang Ho Jeon ),김채기 ( Chae Ki Kim ),송용호 ( Yo 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.3

목적: 류마티스 관절염에서의 염증 자극에 대한 활막 또는 관절액의 fibronectin에 관한 연구는 염증 기전이나 RA의 병인을 규명할 수단이 될 수 있다. 최근에 단클론성 항체의 개발로 체액중 total fibronectin의 측정이 가능하게 되어 RA와 OA의 관절액의 그 농도를 측정하였고, RA 환자를 대상으로 하여 이 측정값의 임상적 가치를 살펴 보고자 하였다. 방법: 무릎 관절 종창을 보이는 RA 및 OA 환자 68명을 대상으로 하여 약 2mL의 관절액을 채취하고 total fibronectin을 단클론성 항체를 이용하여 측정하였고, RA 환자 38명을 대상으로 6가지 기존의 질병활성도를 횡단면연구로 조사하였다. 결과: 1. RA 환자와 OA 환자의 관절액내 total fibronectin의 농도(mean±SD)는, RA가 148.4±72.6μg/ml, OA가 39.5±16.9μg/ml로 OA 환자에 비하여 RA 환자에서 유의하게 증가되어 있다 (p<0.001). 2. RA 환자의 관절액내 total fibronectin의 농도는 질병이환 기간(r=-0.272, p=0.199), 조조강직 기간(r=0.252, p=0.313), ESR (r=0.082, p=0.711), Ritchie index (r=0.217, p=0.359), CRP (r=0.131, p=0.550), 류마티스 인자(r=-0.010,p=0.963)와는 유의한 연관성을 보이지 못했다. 결론: 이상에서 RA 환자에서 관절액 중 total fibronectin의 농도는 OA 환자에서 보다 명확히 높기 때문에 원발성 OA 환자의 그것과 병인론적으로 구별됨을 알 수 있었으나, 임상적으로는 RA의 활성도를 나타내는 지표로 쓰일 수는 없다고 생각된다. Objective: A study on fibronectin, which is synthesized in response to inflammatory process of joint destruction, can be of great value in identifying the mechanism of inflammation or disease activity of rheumatoid arthritis (RA). This study attempts to measure the concentrations of total fibronectin in synovial fluid of patients with RA and osteoarthritis (OA), and compare it with the clinical disease activity parameters of RA available. Methods: A total 68 patients suffering from knee pain and joint effusion was examined. Synovial fluids of thirty-eight RA patients and thirty OA patients were measured by using monoclonal fibronectin antibody. Cross-sectional analysis was undertaken to correlate the fibronectin levels of the RA patients with the clinical disease activity parameters available. Results: 1. Mean synovial fibronectin level of RA (148.4±72.6㎍/ml) was significantly higher than that of OA (39.5±16.9㎍/ml)(p<0.001). 2. The fibronectin levels in RA do not seem to have significant relationship with the parameters such as disease duration, the duration of morning stiffness, Ritchie index, ESR, CRP, and rheumatoid factor. Conclusion: In conclusion, the synovial total fibronectin concentration can clearly distinguish RA from OA. However, it would be unlikely to be used as a parameter of disease activity.

논문 리뷰를 통한 암성통증에 대한 침을 이용한 양한방 통합치료 효과 연구

곽상규,손기철,신임희,김상경,정현정,이아진,조윤정,김달호,Kwak, Sang Gyu,Sohn, Ki Cheul,Shin, Im Hee,Kim, Sang Gyung,Jung, Hyun-Jung,Lee, A-Jin,Cho, Yoon-Jeong,Kim, Dal Ho 한국데이터정보과학회 2015 한국데이터정보과학회지 Vol.26 No.6

난치성 암환자의 암성통증은 암환자의 삶의 질을 지극히 떨어뜨리는 중요한 요인이다. 전 세계적으로 암성 통증을 줄이기 위한 노력으로 양방과 한방의 통합적 치료방법이 증가하는 추세이다. 국내외 적으로도 통합적 접근을 통한 암성통증과 관련된 연구가 지속적으로 이루어지고 있으며 암 치료에 있어서는 화학요법으로 인한 오심이나 구토를 조절하기 위해 표준화된 항구토제와 병용한 침 치료가 효과적임을 보고한 무작위대조군 임상연구가 있다. 여러 연구에서 암으로 인한 피로, 방사선 치료로 인한 구강 건조증, 그리고 불면, 불안, 삶의 질 등에 침치료가 효과적이라고 보고한 바 있다. 그러나 침치료의 실제적인 임상적 효과와 다양한 증례 보고 에도 불구하고, 침을 이용한 통합치료의 진통 감소효과의 유의성에 대해서는 아직 이견이 많다. 이에 따라 본 연구에서는 전 세계 출판된 논문검색을 통하여 침을 이용한 양 한방 통합치료로 인해 암성통증의 감소효과를 파악하고 각 논문에서 제시하는 값을 종합하여 침을 이용한 통합치료의 효과가 어느 정도인지를 평가하기 보았다. Cancer pain is a very important factor in cancer patients refractory to drop the quality of life of cancer patients. The worldwide trend is an integrated effort by both the western medicine and korean traditional medicine of treatment increases to reduce cancer pain. There are many studies related to cancer pain through an integrated medicine approach. Many study was reported that acupuncture treatment is effective for fatigue, xerostomia, insomnia, anxiety and quality of life. However, despite the practical clinical effects and various case reports of acupuncture, many still disagree about the significance of an integrated treatment of pain reduction with acupuncture. Therefore, we has identified that reduce effect of comprehensive and integrative treatment using acupuncture for cancer pain through publication review. And we evaluated effect of comprehensive and integrative treatment using acupuncture through summary of values in each publication.