공배양의 작용기전에 관한 연구

김미경,주보선,김미선,문화숙,이규섭,김한도,Kim, Mi-Kyoung,Joo, Bo-Sun,Kim, Mi-Sun,Moon, Hwa-Sook,Lee, Kyu-Sup,Kim, Han-Do 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: A number of studies to improve in vitro culture conditions have been tried over past ten years by using co-culture system with helper somatic cells. However, the mechanism of coculture is poorly understood. This study was designed to understand the mechanism for the mode of actual action of co-culture using co-culture system of ICR strain's 1-cell embryos with human oviduct epithelial cells by examining the effect of conditioned medium and contactless coculture using a cell culture insert on the embryo development and by measuring the level of superoxide anion from conditioned medium after co-culture. Methods: ICR strain's zygote embryos were cultured in medium alone (control), coculture, conditioned medium, or contactless coculture system for 6 days. Conditioned media (CM) were prepared as following 5 groups. All CM were collected after culturing oviduct cells for 2 days. CM-1 was stored at $-20^{\circ}C$ until use, and CM-2 was prepared just before use as a culture medium. CM-3 was cocultured with embryos and retrieved just before use. CM-4 and CM-5 were derives from the microfilteration of CM-2 and CM-3, respectively, using Microcon-10 (10 kDa molecular weight cut-off). The percentage of the embryos developed to hatched blastocyst stage and the level of superoxide anion in supernatant from medium alone culture (control), coculture, and contactless coculture were measured. Results: The rates of embryo development to the hatched blastocyst stage were significantly higher in coculture (43%) than in control (0%) (p<0.05). The CM-1 group had no embryo development since 2-cell embryonic stage, whereas the CM-2, CM-3, CM-4 and CM-5 groups had the improved development to 4 or 8 cell embryo stage, but the similar rate of development to hatched blastocyst compared to control. The effect of coculture on embryo development was disappeared in the contactless coculture group. The level of superoxide anion was significantly reduced in coculture group compared to control. Conclusion: It is concluded that the present coculture system overcomes the 2-cell block in vitro and improves the embryo development. This beneficial effect may be due to the direct cell-cell contact between embryo and helper cells or the removal of deleterious components from medium rather than the embryotrophic factors.

배양액의 염도에 따른 남조식물 ( SG 63 ) 의 생장 연구

김미경,Jean Claude Thomas (Mi Kyoung Kim) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1

The characteristic of Cyanophyte genus SG63 is similar to that of Aphanot hece sp. The optimal growth was found with the concentration of NaCl and MgSO_4·7H_2O on the culture medium. The most optimal condition is 56‰of NaCl (S4 medium) and 20‰of MgSO_4·7H_2O (M2 medium). The synthesis of chorophyll α, phycocyanin and soluble proteins is affected by the concentration of the two mineral elements in culture. Especially, the content of chlorophyll α and phycocyanin decreases on the most highly saline medium. The identified principal carotenoids are β-carotene, echinenone, zeaxanthin and myxoxanthophyll. The rates of concentration of protein/chlorophyll α and phycocyanin/chlorophyll α are low on the S4 medium. Inversely, these rates are the highest on the M2 medium. Accordingly, the high concentration of MgSO_4·7H_2O provoke the synthesis of phycocyanin and total proteins.

집중력, 청각반응속도, 순발력이 민첩성에 미치는 영향

김미경,남지윤,노수영,한동욱,Kim, Mi-Kyoung,Nam, Ji-Yoon,Noh, Su-Yeong,Han, Dong-Wook 대한물리치료과학회 2012 대한물리치료과학회지 Vol.19 No.3

Background : The purpose of this study was to investigate the relationship between an agility and a concentration, an auditory reaction time and a muscle power. Methods : Twenty one healthy female college students who were 20-21 years participated in the experiment; after being introduced to the methods, they voluntarily signed a consent form. And they received several test related with this study; side step test for an agility, Harris & Harris test for a concentration, brain auditory evoked potential test for an auditory reaction time and vertical jump test for a muscle power. Result : There was no relationship between the agility and the concentration and the auditory reaction time. However, the muscle power related with the agility significantly. Conclusion : In conclusion, these findings suggest that a muscle power training is a good way to promote an agility. So, we can find out that, in an player who requires an agility, a muscle power training is the most important way for promoting an agility than a concentration training or an auditory reaction time training.

유럽 컨테이너 건축물의 사례분석을 통한 국내 적용방안

김미경,문영아,한수지,Kim, Mi-Kyoung,Mun, Young-A,Han, Su-Ji 한국주거학회 2015 한국주거학회 논문집 Vol.26 No.1

The purpose of this study was to analyze the planning characteristics and it's application of container architecture as case study. Field survey was used to analyze the spatial planning characteristics in terms of development outline, appearance, exterior, floor plan and interior of eight famous cases in Berlin, Hamburg, Hannover, Amsterdam and Paris of Europe. The results of this study were as follows; Firstly, good examples of container architectures such as student housing, social service center, temporary medical facility and cruise terminal in Europe suggested the potential of domestic applicability in various purposes and development. Secondly, various types of freight container, building container and module frame system should be developed with their reprocessing environment. Thirdly, it is necessary for us to develop ISO type(20~40ft) container and standard plan with interior and storage design reflecting demands of residents. Finally, the use of container module will be an environmental-friendly alternative for its modularity and reusability, so it should be used as it is without severe deformation. The development of environmental friendly energy sources such as hydro and solar power is necessary for domestic container architecture as well. The container design should include the use of high quality of exterior finishing materials and the plan of aesthetical color planning to make the building a local landmark.

현대집합주거계획에서 비정주성이 표현된 공간구성 특성에 관한 연구

김미경,Kim, Mi-Kyoung 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.4

The purpose of this study is to analyze the characteristics of the non-sedentary in contemporary multi-housing planning through the understanding and interpretation of the modern nomadic life. A document research method was used to analyze and classified the spatial characteristics and development of the non-sedentary dwelling spaces since 1980. Finally, today the applicable cases of non-sedentary space are as follows; the sharing housing, the adapting housing, and temporary housing for the multi-purpose spaces, huge spaces and the industrialization housing. First of all, in order to conform the concept of nomadism, the theory and characteristics were divided into three aspects; The concept of thought of lines, events and Rhisome, and the spatial characteristics of fluidity, multiplicity and the non-hierarchy, that is mainly based on the philosophy of Gilles Deleuze. This study attempted to analyze how the concept of non-sedentary dwelling space has been developed in what form and method they have been applied and interpreted in the historical background. It attempted to resolve the concept of movement and mobility through the experiment so that they might apply to the space of contemporary city.

일시적 거주개념을 적용한 1인용 소형주택의 공간구축유형 및 특성

김미경,송애희,Kim, Mi-Kyoung,Song, Ae-Hee 한국주거학회 2012 한국주거학회 논문집 Vol.23 No.2

The purpose of this study was to analyze the types and characteristics of space construction in temporary small-sized housing for single-person household through the understanding and interpretation of the modern flexible lifestyle. A document research method and case studies were used to analyze and classified the spatial characteristics of temporary dwelling spaces since 2000. Findings of the study were as follows: In order to conform the concept of temporary dwelling for small-sized housing units of single-person households, the types and characteristics of space construction were divided into three aspects: (1) 'Transporting' by wheel, rotation and vehicles, (2) 'Transforming' by adapting, assembling disassembling, and folding unfolding, (3) 'Wearing Carrying' by inflatable and tented type. In conclusion, this study found two types of space construction in temporary small-sized housing. The first was 'formal aspect' which was focused on the simplicity of shape, ease of deformation and lightweight of materials. And the second was 'functional aspect' which was focused on the complex space composition, the rapidity of installation and dismantling, ease of movement and transportation. This study shows that the combination of two types of the temporality will be more ideal in temporary small-sized housing planning rather than relying on just one type.

단일 조성 실온 경화형 실리콘러버를 지지체로 사용한 고체상 탄산이온선택성 전극의 개발

김미경,윤인준,조성호,신혜라,한종호,하정한,남학현,차근식,Kim, Mi-Kyoung,Yoon, In-Jun,Cho, Sung-Ho,Shin, Hye-Ra,Han, Jong-Ho,Ha, Jeong-Han,Nam, Hak-Hyun,Cha, Geun-Sig 대한화학회 2004 대한화학회지 Vol.48 No.3

소형 고체상 전극에 단일 조성 실온 경화형(one-component room temperature vulcanizing type) 실리콘러버 730(730 RTV)을 지지체로 도입하여 가소제가 필요 없는 탄산이온선택성 전극막을 개발하였다. 본 연구에서 최적화된 탄산이온선택성 전극막의 조성은 85.75 wt%의 730 RTV, 11.08 wt%의 trifluoroacetyl-p-decylbenzene(TFADB), 3.17 wt%의 tridodecyl-methylammonium chloride(TDMACl)로 제조 되었고, 우수한 전기화학적 특성(감응기울기 (26.3 mV/dec.), 선택성($logKT^{pot}_{CO_{2},Cl^-}$= -4.00, $logKT^{pot}_{TCO_{2},Sal^-}$=1.69), 검출한계(4.0${\times}10^{-4}M$))을 보였으며, 이와 같은 조성의 막을 도입한 평면형 고체상 센서는 60일 이상 초기특성을 유지하였다. We developed a miniaturized solid-state carbonate ion-selective electrode (carbonate ISE) based on one-component room temperature vulcanizing type silicone rubber 730 (730 RTV) without adding plasticizer to the matrix. The optimized carbonate ion selective membrane is prepared with 85.8 wt% of 730 RTV, 11.1 wt% of trifluoroacetyl-p-decylbenzene (TFADB), and 3.1 wt% of tridodecyl-methylammonium chloride (TDMACl). This carbonate ISE exhibited excellent potentiometric properties (i.e., slope: 26.3 mV/dec; selectivity: $logKT^{pot}_{CO_{2},Cl^-}$= -4.00 and $logKT^{pot}_{TCO_{2},Sal^-}$=1.69); and detection limit for $TCO_2:\;4.0{\times}10^{-4}M$). In addition, the early potentiometric properties of the solid-state sensor with optimized membrane composition were not deteriorated for more than 60 days.

시각적 인지분석을 통한 모바일폰의 향후 표면처리디자인 방향에 관한 연구

김미경(Kim Mi-Kyoung),박경진(Park Kyung-Jin) 한국디자인학회 2007 한국디자인학회 학술발표대회 논문집 Vol.- No.-

자본시장법 시행 전·후 생명보험 산업의 상대적 효율성 및 생산성

김미경(Mi-Kyoung Kim),박희정(Hee-Jung Park),강호정(Ho-Jung Kang) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.8

본 연구는 생명보험산업의 자본시장법 시행 전 2005년부터 2008년까지의 자료와 시행 후 2009년부터 2012년까지의 자료를 토대로 첫째, 자료포락분석을 이용하여 자본시장법 시행 전?후의 효율성 분석을 하고, 대응표본 t-검정을 통해 시행 전?후의 효율성 차이를 살펴보았다. 둘째, 맘퀴스트 지수를 이용하여 자본 시장법 시행 전?후의 생산성 변화를 분석하고 대응표본 t-검정을 통해 생산성 변화 차이를 파악하였다. 셋째, 토빗 회귀분석을 이용하여 자본시장법 시행 전?후 효율성 결정요인을 분석하였다. 본 연구결과는 첫째, 자본시장법 시행 전?후 효율성 평균의 차이가 통계적으로 유의적인 차이가 없는 것으로 나타났다. 둘째, 자본시장법 시행 전?후 생산성 변화의 차이가 5% 수준에서 통계적으로 유의적인 차이가 나타나 시행 후 생산성이 증가하였음을 보여주고 있다. 셋째, 토빗 회귀분석 결과를 보면 설계사 비중이 자본시장법 시행 전? 후의 효율성에 통계적으로 유의적인 정의 영향을 미치는 것으로 나타났다. Purposes of this study are to find difference in efficiency, productivity change and determinants on efficiency of life insurance industry between pre and post-execution of capital market law on the basis of pre(2005-2008) and post-execution(2009-2012) data. Main results of this research are as follows. First, there was no significant difference of mean value for efficiency between pre and post execution of capital market law. Second, difference of mean value for malmquist index between pre and post execution of capital market law was statistically significant at 5% level. This imply increase in productivity for post-execution of capital market law. Third, one of the important determinant for efficiency was the weight of life planner in both pre and post-execution of capital market law. The weight of life planner had significant positive effect on efficiency.

공공안전통신망 단말기 보안성 시험 방법에 관한 연구

김미경(Kim Mi Kyoung),박선우(Park Sun Woo),이태호(Lee Tae Ho),조영진(Cho Young Jin),박준우(Park Jun Woo) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.11