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Pseudomonas aeruginosa KS47에 의한 절삭유의 생물학적 분해
김란희,이상섭,Kim, Lan-Hee,Lee, Sang-Seob 한국미생물학회 2008 미생물학회지 Vol.44 No.1
Cutting oils are emulsionable fluids widely used in metal working processes. Their composition is mineral oil, water, and additives (fatty acids, surfactants, biocides, etc.) generating a toxic waste after a long use. Cutting oils also affect colour, taste and odour of water, making it undesirable for domestic and industrial uses. In these days, conventional treatment methods as evaporation, membrane separation or chemical separation have major disadvantages since they generate a concentrated stream that is more harmful than the original waste. In this study, our purpose is to reduce cutting oils by using biological treatment. Eighty one strains were isolated from cutting waste oil of industrial waste water sludge under aerobic conditions. Among these strains, KS47, which removed 90.4% cutting oil in 48 hr, was obtained by screening test under aerobic conditions(pH 7, $28^{\circ}C$). KS47 was identified as Pseudomonas aeruginosa according to morphological, physiological and biochemical properties, 16S rDNA sequence, and fatty acid analysis. P. aeruginosa KS47 could utilize cutting oil as carbon source. In batch test, we obtained optimal degradation conditions(1.5 g/L cell concentration, pH 7, and temperature $30^{\circ}C$). Under the optimal conditions, 1,060 mg/L cutting oil was removed 83.7% (74.1 mg/L/hr). 본 실험은 생분해가 어려운 절삭유를 단일 균주에 의해 생물학적 처리를 하는 데에 목적이 있다. 절삭유, 절삭폐유로부터 호기 균주 81개를 분리하여 이중 절삭유 분해능이 가장 높은 균주로, 48시간 내에 90.4%를 제거한(초기농도 699.1 mg/L) KS47을 선별하였다. KS47은 형태학적, 생리 화학적, 16S rDNA 염기서열, 그리고 지방산 분석을 통해 Pseudomonas aeruginosa로 동정되었다. P. aeruginosa KS47은 절삭유를 탄소원으로 사용하여 성장 할 수 있었으며, 절삭유 분해시, 최적 분해 조건은 1.5 g/L(wet weight), pH 7.0, $30^{\circ}C$이었다. 최적 조건 하에서 절삭유의 제거능을 본 결과, 1,060 mg/L의 절삭유를 12시간 내에 83.7% 제거함을 확인하였다.
하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출
김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp<sup>®</sup> viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq<sup>®</sup> probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.
김란희 ( Kim Lan Hee ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
2020년 현재 전 세계적으로 창궐한 신종코로나 바이러스(SARS-CoV-2)가 각국에 미친 경제적/문화적/사회적 파급효과는 유래를 찾아보기 어려울 지경이다. 특히 신종 코로나 바이러스는 무증상 감염자도 타인을 감염 시킬 수 있기 때문에 지역 감염을 막는 역학 조사를 실시하는데 매우 큰 어려움이 있다. 최근 해외에서는 하수기반 역학(Wastewater Based- Epidemiology)기법을 이용하여 신종코로나의 지역에서의 증가를 대비하려는 노력이 매우 급속하게 진행되고 있고 이에 대한 일부 성과도 얻고 있다. 본 발표에서는 시민들의 생활상 및 건강지표를 개인적 사생활에 대한 침범 없이 모니터링 할 수 있는 하수 역학에 대해 간단히 살펴보고, 신종코로나 바이러스를 모니터링을 적절하게 하기 위한 여러 과제에 대해 살펴 볼 예정이다. 주로 다룰 내용은 하수에서 측정하는 샘플링 방법, 전처리 방법 및 이를 제도화하기 위한 지자체간의 협력적 방안 등에 대해 발표하려고 한다.
하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출
김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp<sup>®</sup> viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq<sup>®</sup> probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.
하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출
김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-
Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp<sup>®</sup> viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq<sup>®</sup> probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.
Membrane autopsy를 통한 BWRO (Brackish Water Reverse Osmosis) 막오염에 대한 연구
김성조 ( Sung Jo Kim ),김란희 ( Lan Hee Kim ),김창민 ( Chang Min Kim ),신명섭 ( Myung Seop Shin ),유혜원 ( Hye Weon Yu ),오병수 ( Byung Soo Oh ),계정일 ( Jeong Il Kye ),김민정 ( Min Joung Kim ),김인수 ( In S. Kim ) 한국물환경학회(구 한국수질보전학회) 2011 공동 추계학술발표회 Vol.2011 No.-