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김도영,Hyun Chul Kim,Sun Young Kim,이영하 한국미생물학회 2005 The journal of microbiology Vol.43 No.3
A bacterial strain M4-7 capable of degrading various polyesters, such as poly(ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate),was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG,in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions. A bacterial strain M4-7 capable of degrading various polyesters, such as poly (ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG, in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.
광검출기 응용을 위하여 스퍼터된 미세결정 SiGe 박막성장 연구
김도영,김선조,김형준,한상윤,송준호,Kim, Do-Young,Kim, Sun-Jo,Kim, Hyung-Jun,Han, Sang-Youn,Song, Jun-Ho 한국전기전자재료학회 2012 전기전자재료학회논문지 Vol.25 No.6
For the application of photo-detector as active layer, we have studied how to deposit SiGe thin film using an independent Si target and Ge target, respectively. Both targets were synthesized by purity of 99.999%. Plasma generators were generated by radio frequency (rf, 13.56 MHz) and direct current (dc) power. When Ge and Si targets were sputtered by dc and rf power, respectively, we could observe the growth of highly crystalline Ge thin film at the temperature of $400^{\circ}C$ from the result of raman spectroscopy and X-ray diffraction method. However, SiGe thin film did not deposit above method. Inversely, we changed target position like that Ge and Si targets were sputtered by rf and dc power, respectively. Although Ge crystalline growth without Si target sputtering deteriorated considerably, the growth of SiGe thin film was observed with increase of Si dc power. SiGe thin film was evaluated as microcrystalline phase which included (111) and (220) plane by X-ray diffraction method.
치조정 골흡수에 대한 platform switching이 미치는 영향
김도영,김태일,설양조,이용무,구영,류인철,정종평,한수부,Kim, Do-Young,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul,Chung, Chong-Pyoung,Han, Soo-Boo 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.2
Purpose: Numerous studies have shown that crestal bone resorption around the implant was related to the location of the implant abutment junction(IAJ). Recently it was hypothesized that platform switching termed the inward horizontal repositioning of the IAJ might limit bone resorption around the implants. The purpose of this clinical study was to evaluate the effect of platform switching on crestal bone resorption. Materials and Methods: The crestal bone loss of 65 external hex implants in 26 patients were radiographically measured at crown placement and follow-up examinations. 23 standard implants(non-platform switching group, NP) were connected with the matching abutments and 42 wide implants(platform switching group, PS) were connected with the 1 mm smaller diameter abutments. Results: There was significant difference of crestal bone loss between NP group and PS group. For implants in the NP group, mean crestal bone loss was $1.18{\pm}0.68\;mm$ at crown placement and $1.42{\pm}0.41\;mm$ at follow-up. The meal bone loss in PS group was $0.47{\pm}0.52\;mm$ at crown placement and $0.60{\pm}0.65\;mm$ at follow-up. When the crestal bone changes according to placement depths of implants were compared, subcrestal position of IAJ had a significantly less bone loss in PS group, but it was not in NP group. Conclusion: Within the limits of the present study, it was concluded that platform switching technique might decrease crestal bone loss around the implants. Additionally, when the IAJ of implant was placed 1 mm deeper in the alveolar bone, the effect of platform switching on bone loss was enhanced.
만성 견봉하 및 삼각근하 점액낭염에 발생한 다발성 쌀소체의 수술적 치료: 증례 보고
김도영,황정택,이상수,서은민,조윤걸,Kim, Do-Young,Hwang, Jung-Taek,Lee, Sang-Soo,Seo, Eun-Min,Jo, Yoon-Geol 대한근골격종양학회 2013 대한골관절종양학회지 Vol.19 No.2
견봉하 및 삼각근하 만성 점액낭염에 발생한 다발성 쌀소체의 치료에 대해 고찰하였다. 다발성 쌀소체의 형성은 혈청음성의 류마티스 관절염 혹은 결핵과 자주 연관된 만성 점액낭염의 합병증으로 형성된다. 또한 이것은 윤활막 뼈연골종증과 영상학적 및 임상적으로 비슷한 양상을 보인다. 저자들은 만성 삼각근하 및 견봉하 점액낭염에 발생한 다발성 쌀소체가 있어 관혈적 수술을 통해 제거한 44세 남자를 증례 보고하고자 한다. 제거술을 시행한 지 16개월 후 견관절의 운동 범위 및 근력은 정상 범위에 있었고 기능도 만족스러웠다. 일반 촬영 및 초음파 검사에서 쌀소체의 재발은 없었다. 견봉하 및 삼각근하 만성 점액낭염에 발생한 다발성 쌀소체는 점액낭을 포함한 관혈적 절제술에 의해 성공적으로 치료 될 수 있었다. Multiple rice body formation is a complication of chronic bursitis frequently associated with seronegative rheumatoid arthritis or tuberculosis. It resembles synovial chondromatosis on imaging and clinically. We report on a pathologically diagnosed multiple rice body formation in subacromial and subdeltoid bursitis in a 44-year-old man who was treated by surgical removal and bursectomy. At 16 months after the removal, range of motion of affected shoulder was normal. No evidence of recurrence of rice body in plain X-ray and ultrasonography. Multiple rice body formed in chronic subacromial and subdeltoid bursitis could be treated with surgical removal and bursectomy successfully.
실무 3D 스캐닝 및 BIM 활용을 위한 발주자 - 실무자 간 협업프로세스 모델
김도영,Kim, Do-Young 한국BIM학회 2021 KIBIM Magazine Vol.11 No.2
In construction sites, policies are changing considering the convergence of 3D scanning and BIM. In order to respond to this, it is urgent to develop guidelines for systematic collaboration methods that take into account the perspectives of practitioners. By participating in the delivery process using 3D scanning technology, tasks such as ordering, field scanning are defined in terms of mutual communications. Also, the collaboration process is about communications between off-site and on-site, such as feed-back using data and documents. In the future, we will propose guidelines based on such collaborative process models.
급성 족근 관절 원위 경비인대결합 손상에서 TightRope<sup>TM</sup>를 이용한 수술의 단기 치료 결과
김도영,이준혁,박정현,조재호,Kim, Do Young,Lee, Jun Hyuck,Park, Jung Hyun,Cho, Jaeho 대한족부족관절학회 2016 대한족부족관절학회지 Vol.20 No.4
Purpose: The purpose of this study was to evaluate the clinical and radiologic outcome of syndesmosis fixation using TightRope$^{TM}$ (Arthrex, Naples, FL, USA) in acute syndesmosis injuries. Materials and Methods: Twenty-five consecutive patients with acute syndesmosis injuries, treated using TightRope$^{TM}$, were reviewed. Patients were evaluated preoperatively and at the last follow-up (at least 12 months postoperatively). Clinical outcomes were assessed using American Orthopaedics Foot and Ankle Society (AOFAS) ankle-hindfoot score and self-subjective satisfaction survey. Three radiologic parameters were evaluated two times at the preoperative and final follow up from the nonweightbearing ankle anteroposterior radiographs. Results: The mean AOFAS ankle-hindfoot score was 95.5 at the final follow-up. According to the satisfaction survey, 21 patients chose excellent, and four patients chose good. All radiologic parameters, including the mean tibiofibular clear space, mean tibiofibular overlap, and mean medial clear space on nonweightbearing ankle anteroposterior view, significantly improved after surgery. Complications occurred in only one patient who experienced knot irritation with infection. Conclusion: The short-term surgical results of syndesmosis fixation using TightRope$^{TM}$ were good to excellent, both clinically and radiographically. These results suggest that the fixation using TightRope$^{TM}$ is a valid option for acute syndesmosis injury.
스토리텔링을 통한 DMZ관광 상품화에 관한 탐색적 연구
김도영,Kim, Do Young 한국벤처창업학회 2015 벤처창업연구 Vol.10 No.1
본 연구는 평화 안보 문화 역사 생태관광측면에서 중요시되고 있는 DMZ지역 관광 상품화를 위하여 DMZ관광 시장전문가, 관광종사원등에 의한 조사를 실시하였다. 그리고 스토리텔링을 통한 DMZ관광 상품개발을 위해 필요한 중요사항과 DMZ관광 환경형성, 유치 및 개선방안에 대하여 실증 연구하였다. 연구 분석결과 DMZ지역별 관광테마 스토리를 주제로 한 독창적인 관광 상품개발과 지역특성을 고려한 다양한 관광 상품개발이 필요하다고 분석되었다. 또한 지속적인 DMZ관광 이미지 개선 노력과 아울러 스토리텔링 상품의 브랜드화를 통한 마케팅 전략 수립과 관련 관공서 및 협회의 대내 외적 홍보 등이 중요한 것으로 조사되었다. 그리고 DMZ관광객을 위한 수용태세와 관련하여 중요하게 고려하는 사항은 숙박 이용, 음식제공과 특산품 쇼핑 및 기타 쇼핑 상품 구매에 대한 문제점 파악이 시급한 것으로 나타났다. 아울러 DMZ관광 시장 전문인력 양성과 종사원의 서비스 질 향상, 교통 및 접근의 편리성을 위한 수용태세 개선이 필요한 것으로 분석되었다. 그리고 중앙정부의 지원노력(예산배정, 정책 제도개선)이 적극적으로 필요하며 DMZ관광 유치관련 업체에 대한 재정적 인센티브제도(세제혜택) 및 DMZ관광 가격의 투명성, 경쟁력 제고, DMZ 및 민통선 출입절차 개선 등 관광 환경적 형성에 대한 노력이 중요하다고 분석되었다. The purpose of this study is to a study on the development of the DMZ tourism merchandising which use storytelling. also this study is to develop DMZ tourism imagery using the storytelling structured the subject matters based on DMZ. storytelling tourism is the oldest and the best factor of tourism activity among induced factors in these days. As a life of human being is upgrading, the importance of DMZ storytelling tourism merchandising will be magnified. Even tourism commercialization is able to make an achievement as an economical effectiveness to a developing country. Data were collected from 205 members of leading tourism business and job performance in travel agency. data were statistically analyzed using SPSS 20.0. Findings from this study suggest that local government and DMZ tourist business operators consider the storytelling for vitalizing authenticity to improve tourists' satisfaction. also this study is about DMZ tourism commercialization to achieve various opinions of several scholars.