중심압축을 받는 고강도강 중간주의 좌굴강도 평가

김대경,이철호,한규홍,김진호,이승은,김진원,Kim, Dae Kyung,Lee, Cheol Ho,Han, Kyu Hong,Kim, Jin Ho,Lee, Seung Eun,Kim, Jin Won 한국강구조학회 2015 韓國鋼構造學會 論文集 Vol.27 No.4

In this study, inelastic buckling strength of HSA800 steel columns were tested to investigate the applicability of current column curve (KBC2009, 2010 AISC-LRFD) to HSA800 steel. Key test variables included width-thickness ratio, plate-edge restraints, and column slenderness ratio. Specimens made of ordinary steel (SM490) were also tested for comparison purposes. All the concentrically loaded HSA800 specimens exhibited sufficient buckling strength compared to the current column curve, but some of SM490 specimens showed understrength. This difference can be explained by the lower residual stress present in HSA800 specimens. 본 연구에서는 공칭인장강도 800MPa급 고강도강재(HSA800)로 제작된 중간주의 중심압축실험을 통해 좌굴강도를 평가하여 현행 구조설계기준(KBC2009, 2010 AISC-LRFD) 기둥곡선의 비탄성영역 적용성 여부를 검토하였다. 강도로 무차원화한 판폭두께비와 판단부 지지조건 및 기둥 세장비를 변수로 하였으며, 고강도강재와 일반강재의 좌굴거동 차이 여부를 확인하기 위해 일반강재(SM490)로 제작된 비교실험체를 포함시켰다. 실험결과 중심압축을 받는 모든 HSA800 중간주 실험체는 현행 강구조기준의 설계좌굴강도를 충분히 발휘하였으나 일부 SM490 실험체는 설계강도를 하회하였다. 이는 고강도강재의 잔류응력에 의한 강도저하가 일반강재에 비해 작기 때문으로 판단된다.

암모니아 산화 세균 Nitrosomonas sp. PK1의 분리 및 특성

김대경,김현국,이종석,서근학,김성구,공인수,Kim, Dae-Kyung,Kim, Hyun-Kuk,Kim, Jong-Soek,Suh, Kuen-Hack,Kim, Sung-Koo,Kong, In-Soo 한국생명과학회 1997 생명과학회지 Vol.7 No.2

양어장에서 어류의 배설물 및 어류에 의해 소비되지 않고 있는 사료에 의한 암모니아성 질소이 제거를 위한 수처리 공법의 하나로써 질산화세균 및 탈질화 세균을 이용한 고정화 방법의 개발을 위해서는 우수한 암모니아 산화능력이 있는 미생물의 분리가 절대적이다. 이를 위하여 여러 곳으로부터 분리한 암모니아 산화 세균 가운데 생유깅 빠르고 $NO_{2}$ 생성이 뛰어난 Nitrosomonas sp. PK1을 선별하였다. 분리된 균의 생육에 따른 $NO_{2}$ 생성은 non-growth asso-ciated pattern을 보여주고 있었다. 또한 생육에 영향을 미치는 탄소원 가운데 glucose가 생유게 가장 좋았으나 농도가 0.1% 이상에서는 오히려 $NO_{2}$생성이 감소되었다. 따라서 고정화 방범에 이용하기 위해서는 생육과 $NO_{2}$생성 능력이 유지를 위한 적절한 생육 배지의 필요성을 보여주었다. Ion 첨가에 의한 영향을 검토한 결과 $ZnCl_{2}$의 첨가가 생육 및 $NO_{2}$ 생성량의 증가를 보여 주었으며 그 농도는 1~10mM로서 제한적임을 나타내었다. To remove dissolved $NH_{4}$$^{+}$ in the aquaculture system, one ammonia oxidizing bacterium, Nitrsomonas sp. PK1, was isolated from samples collected in many aquacultural place and sludges of waste water. The stationary phase of this atrain was reached after 9 days, and the maximum $NO_{2}$ production was shown from 3 days to 9 days. In the selective medium, 0.1% of glucose was the good carbon source for growth. However, the $NO_{2}$productivity was repressed by the addition of glucose to the medium. When $Zn^{++}$ ion was supplemented to the medium, growth and the $NO_{2}$ productivity was increased, 10mM of $ZnCl_{2}$ was the optimal concentration for growth and 1 mM of $ZnCl_{2}$ was the optimal concentration for the production of $NO_{2}$, respectively.

볼트 전단파단이 지배하는 지압형식 볼트접합부의 힘-변형 관계

김대경,이철호,진승표,윤성환,Kim, Dae Kyung,Lee, Cheol Ho,Jin, Seung Pyo,Yoon, Seong Hwahn 한국강구조학회 2015 韓國鋼構造學會 論文集 Vol.27 No.1

잘 접합된 볼트접합부는 볼트의 전단파단이 발생할 때 까지 지압 메커니즘에 의하여 훌륭한 연성거동을 보인다. 이러한 볼트접합부에 내재된 연성 능력을 최대한 활용한다면 중력하중, 풍하중, 지진하중 등 다양한 하중에 대하여 활용하여 접합비용의 경제성 제고에 기여할 수 있다. 편심하중을 받는 볼트접합부의 경우 보수적인 탄성해석법과 합리적으로 볼트접합부의 연성거동을 활용할 수 있는 극한강도 해석법이 존재하나 기존의 극한강도해석법은 오늘날 다양한 소재와 설계조건의 다양화에도 불구하고 하나의 소재와 조건으로 이루어진 실험식에 의존하고 있다. 본 연구의 주된 목적은 체계화된 단일볼트의 실험을 기반으로 볼트의 일반화된 힘-변형 관계식을 정립하는 것이다. 실험결과를 토대로 볼트접합부의 기하학적인 요소(볼트의 직경, 플레이트의 두께) 및 강도가 힘-변형 관계에 주된 영향을 미치는 요소라는 것을 알 수 있다. 실험결과를 순간중심회전법에 적용하여 보다 정확하고 경제적인 편심하중을 받는 군볼트 접합부 설계가 가능하다. Well-designed bolted connections can exhibit excellent ductile behavior through bearing mechanism until the occurrence of bolt shear rupture. The ultimate strength analysis of eccentric bolted connections is an economical and mechanistic approach which uses such ductility. However, the bolt load-deformation relationship, which forms basis of the current practice, is based on very limited combinations of bolt and steel materials. The primary objective of this study was to establish the general bolt force-deformation relationship based on systematic single-bolt bearing connection tests. The test results showed that the projected area of the bolt hole and the strength and thickness of the plate to be connected are the main factors affecting the force-deformation relationship. The results of this study can be used for the instantaneous center of rotation method (ICRM) to achieve more accurate analysis and economical design of a variety of group-bolted connections subjected to eccentric shear.

Purification and Characterization of Mitochondrial Mg2+-Independent Sphingomyelinase from Rat Brain

김대경,Jong Min Choi,Yongwei Piao,Kyong Hoon Ahn,Seok Kyun Kim,Jong Hoon Won,Jae Hong Lee,In Chul Shin,Zhicheng Fu,Sung Yun Jung,Eui-Man Jeong 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.9

Sphingomyelinase (SMase) catalyzes ceramide production from sphingomyelin. Ceramides are critical in cellular responses such as apoptosis. They enhance mitochondrial outer membrane permeabilization (MOMP) through self-assembly in the mitochondrial outer membrane to form channels that release cytochrome c from intermembrane space (IMS) into the cytosol, triggering caspase-9 activation. However, the SMase involved in MOMP is yet to be identified. Here, we identified a mitochondrial Mg2+-independent SMase (mt-iSMase) from rat brain, which was purified 6,130-fold using a Percoll gradient, pulled down with biotinylated sphingomyelin, and subjected to Mono Q anion exchange. A single peak of mt-iSMase activity was eluted at a molecular mass of approximately 65 kDa using Superose 6 gel filtration. The purified enzyme showed optimal activity at pH of 6.5 and was inhibited by dithiothreitol and Mg2+, Mn2+, N2+, Cu2+, Zn2+, Fe2+, and Fe3+ ions. It was also inhibited by GW4869, which is a non-competitive inhibitor of Mg2+-dependent neutral SMase 2 (encoded by SMPD3), that protects against cytochrome c release-mediated cell death. Subfractionation experiments showed that mt-iSMase localizes in the IMS of the mitochondria, implying that mt-iSMase may play a critical role in generating ceramides for MOMP, cytochrome c release, and apoptosis. These data suggest that the purified enzyme in this study is a novel SMase.

Recombinant Human HAPLN1 Mitigates Pulmonary Emphysema by Increasing TGF-β Receptor I and Sirtuins Levels in Human Alveolar Epithelial Cells

김대경,Yongwei Piao,So Yoon Yun,Zhicheng Fu,Ji Min Jang,Moon Jung Back,Ha Hyung Kim 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.9

Chronic obstructive pulmonary disease (COPD) will be the third leading cause of death worldwide by 2030. One of its components, emphysema, has been defined as a lung disease that irreversibly damages the lungs’ alveoli. Treatment is currently unavailable for emphysema symptoms and complete cure of the disease. Hyaluronan (HA) and proteoglycan link protein 1 (HAPLN1), an HA-binding protein linking HA in the extracellular matrix to stabilize the proteoglycan structure, forms a bulky hydrogel-like aggregate. Studies on the biological role of the full-length HAPLN1, a simple structure-stabilizing protein, are limited. Here, we demonstrated for the first time that treating human alveolar epithelial type 2 cells with recombinant human HAPLN1 (rhHAPLN1) increased TGF-β receptor 1 (TGF-β RI) protein levels, but not TGF-β RII, in a CD44-dependent manner with concurrent enhancement of the phosphorylated Smad3 (p-Smad3), but not p-Smad2, upon TGF-β1 stimulation. Furthermore, rhHAPLN1 significantly increased sirtuins levels (i.e., SIRT1/2/6) without TGF-β1 and inhibited acetylated p300 levels that were increased by TGF-β1. rhHAPLN1 is crucial in regulating cellular senescence, including p53, p21, and p16, and inflammation markers such as p-NF-κB and Nrf2. Both senile emphysema mouse model induced via intraperitoneal rhHAPLN1 injections and porcine pancreatic elastase (PPE)-induced COPD mouse model generated via rhHAPLN1-containing aerosols inhalations showed a significantly potent efficacy in reducing alveolar spaces enlargement. Preclinical trials are underway to investigate the effects of inhaled rhHAPLN1-containing aerosols on several COPD animal models.

주기적인 유지보수 계획에 따른 중고제품에 대한 최적 향상수준

김대경,김진우,박동호,Kim, Dae-Kyung,Kim, Jin Woo,Park, Dong Ho 한국통계학회 2013 응용통계연구 Vol.26 No.1

중고제품 특히 고가인 중고품에 대한 점증하는 수요로 인하여 그러한 제품에 대한 보증과 보전정책이 최근 제품의 신뢰성을 향상시키기 위해 연구되고 있다. 이 논문에서는 사용한 년 수 x에서 고객에 의해서 구입된 중고제품에 대한 주기적인 유지보수모형을 연구하였다. 구입할 때 판매자는 제품의 고장율을 줄이기 위해서 그리고 각각의 유지보수가 수행되고 난 이후에 신뢰성을 향상시키기 위해서 정해진 보증기간을 제공한다. 만일 연속적인 유지보수 사이에서 고장이 일어난다면 단지 최소수리가 행해진다. 보증정책에 대해서 보증기간동안에 주기적인 유지보수 점검과 더불어 각 고장에 관해서는 무상 비재생수리를 한다. 따라서 이러한 보증정책 하에서 보증기간에 일어난 모든 유지보수와 수리비용은 판매자에게 부과된다. 제안된 주기적인 유비보수 계획에 대해서 보증기간 동안에 판매자에게 부과된 기대 총비용을 계산하기 위한 모형과 판매자의 측면에서 총기대보증비용을 최소화하기 위한 각 유지보수에서 고장율의 최적향상수준을 유도한다. 또한 제안된 방법들에 근거해서 최적향상수준에 대한 수치적인 결과를 제시한다. Due to a growing demand for the second-hand product, especially for the expensive one, the warranty and maintenance policies for such products have been studied to improve the product reliability of late. In this paper we study a periodic maintenance model for the second-hand product which is purchased by the customer at the age of $x$. When purchased, the dealer provides a warranty of a fixed length during which the product is maintained periodically to reduce the failure rate of the product and thus, to improve the reliability after each maintenance is served. If a failure occurs between two successive maintenances, only minimal repair is conducted. As for the warranty policy, we adopt free non-renewing repair action on each failure, in addition to the periodic maintenance service during the warranty period. Thus, under the given warranty policy, all the maintenance and repair costs incurred during the warranty period are charged to the dealer. For the proposed periodic maintenance scheme, we formulate a cost model to evaluate the expected total cost charged to the dealer during the warranty period and derive an optimal upgrade level of the failure rate at each maintenance to minimize the expected total warranty cost from the perspective of the dealer. We also present numerical results for an optimal upgrade level based on the proposed methods.

논단 2-전기방식 기술

김대경,Kim, Dae-Gyeong 한국전기산업진흥회 1996 電氣産業 Vol.7 No.4

보청기에서 적응궤환제거의 성능 향상

김대경,허종,박장식,손경식,Kim, Dae-Kyung,Hur, Jong,Park, Jang-Sik,Son, Kyung-Sik 한국음향학회 1999 韓國音響學會誌 Vol.18 No.4

본 논문에서는 보청기에서의 적응궤환 제거 성능을 개선하기 위한 방법들을 제안하였다. 첫번째 방법은 순시 경사치를 모니터링하여 최적해를 추적해 가는 것으로 직교원리를 이용한 음향학적 궤환제거 방법이고 다른 하나는 본 실험실에서 제안된 적응 알고리즘인 보상기를 가진 적응알고리즘을 이용한 방법이다. 다양한 시뮬레이션 조건하에서 본 논문에서 제안된 적응 궤환제거 방법이 Greenberg가 제안한 합-방식(Sum-method) 최소자승오차 알고리즘 보다 시스템 부정합, 신호대 잡음비(SNR: Signal-to-Noise Ratio) 및 세그멘트 SNR에서 훨씬 좋은 성능을 나타내었다. 또한 적응 궤환제거에 있어서 직교원리를 이용한 방법은 시뮬레이션에서 보상기를 가진 적응알고리즘을 이용한 방법과 유사한 성능을 나타내었다. In this paper, two methods were proposed to improve the performance of adaptive feedback cancellation in hearing aids. One is “Orthogonality principle acoustic feedback cancellation method(Orthogonality principle method)” to track optimal solution with monitoring the instantaneous gradient, the other is a method using the CLMS algorithm(CLMS method). In many simulation conditions, adaptive feedback cancellation method proposed in this paper was much better than Greenberg method by Sum-method LMS algorithm which is known the most excellent method by now in case of system mismatch, SNR and segmental SMR. Also. Orthogonality principle method is as good as CLMS method in terms of adaptive feedback cancellation in many simulation conditions.

점진적 앉은 자세에서의 PNF운동이 소뇌 손상환자의 동적 균형능력에 미치는 효과 -단일 사례 연구-

김대경,나은진,문상현,Kim, Dae-Kyeong,Na, Eun-Jin,Moon, Sang-Hyun 대한고유수용성신경근촉진법학회 2016 PNF and Movement Vol.14 No.2

Purpose: The purpose of this study was to investigate the effects of using proprioceptive neuromuscular facilitation (PNF) exercise in the progressive sitting position on the dynamic balance ability of a patient with a cerebellar injury. Methods: The subject had ataxia due to cerebellar injury. The subject participated in a PNF bilateral scapular pattern exercise with stabilizing reversal technique during a progressive sitting position session as well as baseline for 20 minutes a day for 4 weeks. In the first session, PNF exercises were performed at a height of 40 cm for 10 minutes, and in the second session they were performed at 50 cm for 10 minutes from a lower center of gravity (COG) to a higher COG sitting position. We used the Berg Balance Scale (BBS), Five-Times-Sit-to-Stand Test (FTSST), and the Timed Up and Go Test (TUGT) to measure the subject's dynamic balance ability every two days through the entire session. Results: After participating in the program, the subject's dynamic balance ability improved compared to the first baseline, as measured by BBS (2 points increased), FTSST (5.3 sec decreased), and TUGT (2 sec decreased). The increase was also maintained in the second baseline session. Conclusion: PNF exercise using bilateral scapula patterns with a stabilizing reversal technique helps to enhance the dynamic balance ability of a cerebellar injury patient.

국가 사이버보안 수준 향상을 위한 GCI의 지표개선 방안

김대경,이주현,김예영,현다은,오흥룡,진병문,염흥열,Kim, Dae kyung,Lee, Ju hyeon,Kim, Ye young,Hyeon, Da eun,Oh, Heung-Ryong,Chin, Byoung moon,Youm, Heung Youl 한국정보보호학회 2022 정보보호학회논문지 Vol.32 No.2

The Global Cybersecurity Index (GCI) developed by the International Telecommunication Union (ITU) is used to diagnose a country's cybersecurity development level and to strengthen its cybersecurity capabilities. This paper analyzes GCI and tries to suggest a way to strengthen its effectiveness. In addition, we analyze the GCI version 1~GCI version 4 evaluation index in advance, and examine the development plan through SWOT analysis. Through this, basic principles for GCI improvement and utilization will be established, and new indicators related to the GCI version 5 questionnaire will be discovered and suggested. This paper is expected to be used as basic data for GCI performance analysis and improvement plan. In addition, it is intended to contribute to enhance the effectiveness of GCI and the nation's cybersecurity capabilities by proposing more advanced proactive and reactive indicators to be applied to the future GCI evaluations. This paper is an improvement and development for the research result of [1].