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서울지역 길고양이의 Toxoplasma 감염증 실태조사
김능희 ( Neung Hee Kim ),채희선 ( Hee Sun Chae ),한혜진 ( Hye Jin Han ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ),김철훈 ( Chul Hun Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3
Toxoplasma gondii is one of the most common protozoa parasites of human and other warm-blooded animals. Cats and wild felidae play crucial roles in the epidemiology of toxoplasmosis. This study was performed to survey the prevalence of T. gondii infection among stray cats in the Seoul. A total of 422 stray cat blood samples were collected from Seoul area. Positive sera for T. gondii were identified in 56 samples (13.3%) exclusively via the latex agglutination test and the detection no antigen particles among seropositive samples by PCR. The overall infection rate of male stray cats (14.4%) presented as higher than that of female cats (10.7%). This study suggested that T. gondii is widespread in stray cats of Seoul area. It is needed to control urban stray cat population and to reduced the risk of zoonotic transmission of toxolasmosis to other animals and humans.
서울지역의 개와 고양이에서 분리한 Staphylococcus aureus의 항생제 감수성 및 multiplex PCR을 이용한 장독소의 검출
김능희 ( Neung Hee Kim ),채희선 ( Hee Sun Chae ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ),김철훈 ( Chul Hun Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
The antimicrobial susceptibility of 73 strains of Staphylococcus aureus isolated from dogs and cats was determined against 16 antimicrobial agents. All isolates were susceptible to cephalothin (CF), vancomycin (VA), rifampin (RA), quinupristin-dalfopristin (SYN) and linezolid (LZD), whereas more than 86.3% of the isolates were resistant to penicillin (P). In the case of enterotoxin production, 8.2% of 73 strains produced enterotoxin A and 1 strain produced enterotoxin C. Appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will need to be investigated in the future.
서울지역 사육 소의 큐열 및 톡소포자충 항체 보유율 조사
김능희 ( Neung Hee Kim ),김혜라 ( Hye Ra Kim ),박형숙 ( Hyung Suk Park ),김영섭 ( Young Sub Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.4
Both Q-fever and Toxoplasmosis are zoonosis. Q-fever occurs due to intracellular bacteria, while Toxoplasmosis is created by protozoan. Both of them have a wide range of host including livestock and wild animals and occur sporadically all over in the world. In this study, seroprevalence of Q-fever and Toxoplasmosis was investigated on cows bred in the area of Seoul where there was a fairly high possibility to occur, while vaccine was not used in Korea. As for experiment materials, cattle blood collected from 190 cows from February to September in 2014 was used ELISA. According to the result, there was a positive reaction on Q-fever from 18 cows out of total 190 cows (9.5%) and on Toxoplasmosis from 32 cows (16.8%). Seroprevalence of both diseases per age was turned out to be negative for those aged less than 2. In addition, it was shown to be positive on 4 cows out of 87 (4.6%) cows aged from 3 to 5, on 7 cows out of 30 cows (23.3%) aged from 6 to 7. Finally, it was shown to be positive on 7 cows out of 17 cows (41.2%) aged 8 or above. Toxoplasmosis was turned out to be positive on 1 cow out of 56 cows (1.8%) aged less than 2, on 6 cows out of 87 cows (6.9%) aged from 3 to 5, on 17 cows out of 30 cows (56.7%) aged from 6 to 7. In addition, it was turned out to be 8 cows out of 17 cows (47.1%) aged 8 or above. Seroprevalence of both diseases was turned out to be higher as age increased. Therefore, it seems that a wide range of investigation on the entire disease spreaders as well as livestock is required since infection of Q-fever and Toxoplasmosis, types of zoonosis, has continuously occurred, and the number of insects, wild animals, and stray animals serving as a role of spreading diseases by changes in seasons and environments has been gradually increasing in Korea.
오리 분변에서의 Campylobacter jejuni 오염도와 항생제 내성유형 조사
김능희 ( Neung Hee Kim ),채희선 ( Hee Sun Chae ),강영일 ( Yong Il Kang ),신박우 ( Bang Woo Shin ),최농훈 ( Nong Hoon Choi ),김효비 ( Hyo Bi Kim ) 한국가축위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.1
This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.
서울지역 동물보호소 내 유기견의 개 심장사상충 감염 실태조사
김능희 ( Neung Hee Kim ),곽정연 ( Jeong Yeon Kwak ),김혜라 ( Hye Ra Kim ),박형숙 ( Hyeong Suk Park ),김두환 ( Doo Hwan Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.3
Dirofilaria immitis infection is one of the most important parasitic diseases in dogs and public health. It often elicits nodules in the pulmonary parenchyma and in the subcutaneous tissues of human. In this study, we investigated the prevalence of D. immitis infection among 754 dogs in Seoul area from April to December 2012. The infection rate of 754 dogs was 9.8% (74/754) by the antigen test (canine SNAP 4Dx). The infection rates of D. immitis in dogs at the age of <2, 2∼4, 5∼7, 8∼10, and >10 years were 3.7% (7/189), 12.9% (30/233), 15.0% (19/127), 9.8% (18/184), and 0% (0/21), respectively. The infection rates in the female and male dogs were 9.7% (33/340) and 9.9% (41/414), respectively. The regional infection rates in northern and southern Han river region of Seoul were 10.7% (44/410) and 8.7% (30/344), respectively. In order to confirm D. immitis infection, we performed PCR on serum samples of 74 dogs which tested positive for D. immitis antigen by the antigen test and we detected a specific gene ITS-2 in 59 serum samples. In conclusion, this study suggests that treatment and preventive care on the D. immitis infection should be considered in dogs of Seoul area.
김능희 ( Neung Hee Kim ),채희선 ( Hee Sun Chae ),손홍락 ( Hong Rak Son ),강영일 ( Young Il Kang ),이정학 ( Jung Hark Lee ),김세곤 ( Se Gon Kim ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
Rabies virus is transmitted most commonly through a bite from an infected dog. Especially, stray dogs that are not excluded from contact with rabid wild animals can become rabies vectors. Therefore, serological survey of rabies virus from stray dogs in Seoul Metropolitan City was carried out in this study. To investigate prevalence of rabies antibodies in the stray dogs, serum samples were taken from 500 stray dogs between April and December 2010. Antibodies to rabies virus were detected by indirect ELISA. Of 500 tested sera, 147 (29.4%) were positive to rabies virus. Prevalence rates of rabies antibodies( PRRA) in northern and southern Han river region of Seoul were 26.4% and 33.2% respectively. PRRA in male and female dogs were 33.6% and 26.1% respectively. PRRA in less than 1 year, 1∼2 < years, 2∼3< years, 3∼5< years, 5∼10< years and over 10 years old dogs were 12.7%, 21.6%, 26.4%, 36.4%, 32.5% and 46.4%, respectively. These results indicates that antibody seroprevalence to rabies is still not enough to prevent rabies and rabies vaccination is required to enhance the seroprevalence in the dogs. To improve the situation, measures to raise public awareness of rabies and its prevention is needed. Also, reducing stray dogs and keeping companion animals from contact with wild animals are indispensable for the prevention of rabies.
소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성
채희선 ( Hee Sun Chae ),김능희 ( Neung Hee Kim ),한혜진 ( Hye Jin Han ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ),김종택 ( Jong Taek Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3
Shiga toxin (Stx)-producing Escherichia coli (STEC) strains can cause broad spectrum of human disease, including diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic colitis (HUS). We examined 868 samples was taken from bovine feces and carcass from January to December 2008 in Seoul. Twenty two (9.5%) shiga toxin -producing Escherichia coli were isolated from the 230 of bovine feces, and two (0.31%) were isolated from the 638 of carcasses. Serotype of E. coli isolates were O157 (10, 41.6%), O26 (10, 41.6%), O111 (1, 4.2%) and UT (3, 12.6%). In PCR, the isolates displayed three different stx gene combination (stx1〔2, 8.4%〕], stx2〔3, 12.6%〕and stx1 and stx2〔19, 87.5%〕). The eaeA and hlyA gene were found in 11 (45.8%) of the 24 strains. Saa gene was present only one strains (4.2%). Toxin typing using reverse passive latex agglutination test showed the same result in VT 1. But it showed different result in VT 2. In antimicrobial susceptibility test, all isolates were sensitive to amikacin, amoxicillin/clavulanic acid, ciprofloxacin and colistin. Eighteen strains (75.0%) of 24 isolates showed the multi-resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty four isolates was digested with Xba I. the 24 isolates showed 7 (A~G) PFGE type.
수생조류에서 Clostridium botulinum type C에 의한 발증사례
채희선 ( Hee Sun Chae ),김능희 ( Neung Hee Kim ),손홍락 ( Hong Rak Son ),김창기 ( Chang Ki Kim ),김선흥 ( Sun Heung Kim ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Botulism in wild waterfowl has been reported in many areas of the world. In 18 October 2008 to 22, 101 dead wild ducks were found in Anyangcheon. 24 of dead birds were spotbill (Anas poeilorhyncha), 56 were Mallard (Anas platyrhynchos), 18 were green- winged teal (Anas carolinensis) and 3 were others. Clinical sign of the sick birds were flaccid paralysis of the neck. At autopsy, most birds had no specific lesions. Stomach is empty. In the mouse bioassay, Samples of serum and intestinal contents taken from 9 dead birds killed all the injected mice, and their toxicity was neutralized by the antitoxin against C. botulinum type C toxin.
R plasmid와 bla Gene을 이용한 병원 내 유행 ${\beta}$-lactam 내성 장내세균의 확인
설성용,원준희,김능희,유학선,이유철,조동택,김정완,Seol, Sung-Yong,Won, June-Hee,Kim, Neung-Hee,Yu, Hak-Sun,Lee, Yoo-Chul,Cho, Dong-Taek,Kim, Jung-Wan 대한미생물학회 2001 Journal of Bacteriology and Virology Vol.31 No.3
Clinical isolates of Enterobacteriaceae (189 Klebsiella, 61 Enterobacter, 32 Serratia, 19 E. coli, 7 Proteus, and 3 Citrobacter) from one university hospital were epidemiologically analyzed by using transferable R plasmids resistant ${\beta}$-lactam antibiotics including broad-spectrum cephalosporins. About 30% of E. cloacae and S. marcescens and about 5% of K. pneumoniae were resistant to one or more broad-spectrum ${\beta}$-lactam antibiotics including cefotaxim, ceftazidime, aztreonam, or cefoxitin but all isolates of E. aerogenes, K. oxytoca, and P. mirabilis were susceptible. Thirty-six conjugative R plasmids including 8 plasmids resistant expanded-spectrum cephalosporins were obtained from multiple resistant K. pneumoniae (19), E. cloacae (9), E. coli (4), and C. freundii (1). Thirty-one plasmids were subjected to R plasmid analysis and classified 20 different plasmid types. Among them 5, 2, and 2 plasmids belong to 3 different types respectively showed identical molecular size, endonuclease fragment pattern by Southern hybridization pattern by TEM-1 probe, pI value by isoelctric focusing, and also identical antibiogram and biotype of wild strains harboring plasmids. But all of plasmids resistant to cefotaxim, ceftazidime, aztreonam or cefoxitin showed different palsmid anlysis patterns. These results indicate that the epidemic strains of 3 clonal types had been present in this hospital and anlysis using transferable R plasmid and bla gene can be used to discriminate multi-resistant clinical isolates of Enterobacteriaceae.
서울지역 교육시설 및 공원 내 토양 중 분원성대장균군 오염실태
김혜라 ( Hye Ra Kim ),김능희 ( Neung Hee Kim ),박형숙 ( Hyong Suk Park ),김두환 ( Doo Hwan Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.4
Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coliform in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most frequently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics using disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramycin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was highly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.