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Lateral Flow Membrane를 이용한 인유두종 바이러스 DNA Array의 개발
김기황,이형구,조홍범,Kim, Ki-Whang,Lee, Hyung-Ku,Cho, Hong-Bum 한국미생물학회 2008 미생물학회지 Vol.44 No.4
본 연구에서는 임신진단등의 간편 현장진단(point of care test, POCT)에 주로 사용되고 있는 멤브레인 측면흐름 분석기법을 사용하여 인유두종 바이러스(Human papillomavirus, HPV)의 특정 서열을 검출할 수 있는 DNA array를 개발하였다. HPV type 6, 11, 16, 18, 31, 45에 특이적인 DNA 탐침들을 측면흐름 분석용 멤브레인 표면에 고정하고, biotin이 label된 MY09/11 primer를 사용하여 얻어진 HPV PCR 반응 결과물과 탐침 사이에 hybridization 반응을 유도하였다. 이후 streptavidin이 label된 colloidal gold가 교잡물의 biotin과 반응함으로써 DNA hybridization 결과를 육안으로 확인할 수 있었다. 본 연구를 통해 개발된 HPV DNA lateral flow membrane array는 기존의 HPV DNA chip 기법과 비교하여 경제적이고 편리하게 주요 HPV type을 확인할 수 있음을 보여주었다. This study develops DNA array which can detect specific sequence of human papilomavirus (HPV) by using lateral flow membrane assay which is usually used for point of care test including pregnant diagnosis. Principle of HPV DNA array is as follow; fixing DNA probe which is peculiar to HPV type 6, 11, 16, 18, 31, 45 on a surface of lateral flow membrane and inducing hybridization response between probe and HPV PCR products which is obtained by using biotin-labeled MY09/l1 primers. And then, we can see the result of DNA hybridization that streptavidin labelled colloidal gold is responded with hybrid biotin. Lateral flow membrane array developed in this study confirms major HPV type economically and conveniently compared with existing HPV DNA chip method.
김기황,정훈채,김용연,이미경,유연현,Kim Ki-Whang,Jeong Hun-Chae,Kim Yong-Yeon,Lee Mee-Kyoung,Yu Yun-Hyun 한국연초학회 2005 한국연초학회지 Vol.27 No.1
Studies on effect of Decyl alcohol EC and Butralin EC on tobacco sucker control were conducted in the tobacco field of Agro-tech Research Group of KT&G Central Research Institute, in 2004. Control effects of two chemicals were highest at the sucker length of 1$1\~2cm$. This indicates that the first application of Decyl alcohol should be conducted before button stage in the flue-cured and at the button stage in the burley tobacco. Effects of Decyl alcohol and Butralin on sucker control were $89.6\%\;and\;90.7\%$, respectively at 14 days after application in the variety KF118. But that of sequential application of two chemicals was $81.9\%$ at 30 days after application in the variety KF118 and $86.1\%$ in the variety KB303. Effect of two applications of Yellow ribbon or Butralin was highest in two varieties. The central downward spray showed a little higher control effect than the pouring of two chemicals. There was not significant difference of sucker control effect between forty and fifty times solution of Decyl alcohol in the variety KB303.
김기황(Ki Whang Kim),이종태(Jong Tae lee),유형식(Hyung Sik Yoon),이도연(Do Yun Lee) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.2
N/A The recent development of various imaging modalities has simplified the diagnosis of hepatocellular carcinoma (HCC). 38 patients with a total of 40 small HCC less than 5 cm were studied with ultrasonography, Computed tomography, Gallium 67 scan, and selective celiac angiography. The characteristic findings on each imaging modalities were analysed and the detection rate of small HCCs by the modalities were assessed. The results as follows; 1) Ultrasonography revealed low leveled internal echo in less than 3 cm tumor without definite halo or lateral shadow, however halo was seen in 73% of masses larger than 3 cm in size. 2) Post contrast enhancement CT demonstrated low attenuation mass lesions in 87.5% of cases and central necrotic components were seen in 18.7% of 5 cm sized HCCs. Extrahepatic extensions or the portal vein thrombus were not observed. 3) Gallium-67 SPECT was more useful to detect small peripheral tumors which were not detected on US or CT. 4) Angiography failed to depict small tumors less than 2 cm in 33%, and A-P shunt was not demonstrated in less than 3 cm tumor and nodular venous staining was one of the characteristic findings of angiography. 5) The detection rate of 16 small HCCs performed all the imaging modalities as follows, ultrasonography, 68.8%, CT, 87.5%, Ga-67 planar, 56.3% SPECT, 81.3% selective celiac angiography, 93.8% respectively. 6) To detect small hepatocellular carcinoma, imaging modalities should be combined not only ultrasonography as well as Ga-67 SPECT and CT as noninvaseve procedures.
문영명(Young Myoung Moon),김기황(Ki Whang Kim),김경희(Kyung Hee Kim),김응(Eung Kim),김복성(Bock Seong Kim),곽승민(Seung Min Kwack) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.2
N/A Tuberculous lymphadenitis within the abdomen is uncommon, and diagnosis is difficult because symptoms are vague, signs nonspecific, and clinical tests often not conclusive. It may mimic a large variety of conditions including lymphoma or metastasis. Recenty we had experienced 4 cases of tuberculous lymphadenitis in the abdomen. Th diagnosis was obtained by ultrasonogrpahy guided fine needle aspiration biopsy, which was less invasive procedure than laparotomy. The clinical suspicions and the extent of the disease were made by abdominal ultrasonogroaphy and computerized tomography. Histologic examination showed granulomas in 2 of 4 cases, and acid-fast bacilli in 3 of 4 cases. All the patients received antituberculous chemotherapy with prompt clinical improvement.
막성 (膜性) Budd - Chiari 증후군의 (症候群) 경피적 (經皮的) 혈관성형술 (血管成形術)
강진경(Jin Kyung Kang),김기황(Ki Whang Kim),최규옥(Kyu Ok Choe),김응(Eung Kim),조범구(Bum Koo Cho) 대한소화기학회 1986 대한소화기학회지 Vol.18 No.2
The author report a case of membraneous type of Budd-Chiari Syndrome which was treated by percutaneous transluminal angioplasty. After procedure, there showed no pressure gradient between RA and IVC. Collateral circulations through left renal and paravertebral vein were disappeared. Clinically, abdominal wall venous collaterals was also disappeared. Percutaneous transluminal angiop1asty will be a promising method in the treatment of membraneous type of Budd-Chiari Syndrome.
남수연(Su Youn Nam),정준표(Jun Pyo Chung),김응(Eung Kim),이종화(Jong Hwa Lee),김기황(Ki Whang Kim),정현주(Hyeon Joo Jeong),정재복(Jae Bock Chung),한지숙(Jee Sook Hahn),강진경(Jin Kyung Kang),박인서(In Suh Park) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.5
Primary pancreatic lyrnphoma is a rare but treatable malignancy that may present as an isolated pancreatic mass. The authors report a case of primary pancreatic lymphoma in a 52- year-old female patient who had a 15-day history of epigastric pain. The CT scan showed a well defined isodense mass, about 5crn in diarneter, in the head of the pancreas and the second portion of duodenum is streched and displaced toward the right side. By percutaneous needle biopsy, histologic diagnosis was made as malignant lymphoma, mixed large and small cells, T cell type according to the Working Formulation. The patient had received combination chemo- therapy with CHOP(cyclophosphaide, doxorubicin, vincristine, predinisone). Epigastric pain and jaundice which was developed during the diagnostic work-up period were improved after one cycle of CHOP. However, the patient died of septic shock on the 56th day after the diag- nosis.(Korean J Gastroenterol 1994; 26: 892 898)
장중첩증이 (腸重疊症) 동반된 대장지방종 (大腸指肪腫)
박인서 ( In Suh Park ),박찬일 ( Chan Il Park ),이경식 ( Kyung Sik Lee ),김기황 ( Ki Whang Kim ),최흥재 ( Heung Jae Choi ),양주영 ( Joo Young Yang ),박창윤 ( Chang Yoon Park ),윤용광 ( Yong Dwang Yoon ) 대한소화기학회 1982 대한소화기학회지 Vol.14 No.1
Excluding adenomataus polyps, lipomas represent the most common benign tumor of the colon. But lipomas of gastrointestinal tract are rarely encountered, and when seen they are usually an incidental finding at autopsy or operation. When small, these tumors are frequently asymptomatic. Constipation and colicky abdominal pain in wax and wane pattern are frequent symptoms in the larger tumor and intussusception is the main complication of the lipomas. A few hundreds of gastrointestinal lipomas were reported in the world, but only 6 cases of it were found in the literature in Korea. A case of lipomas in colon associated with intussusception in a 36-year-old woman and a brief review of the literature were presented.
서정건(Jung Kun Seo),박효진(Hyo Jin Park),김경철(Kyung Chul Kim),이귀순(Kwi Soon Lee),윤상욱(Sang Wook Yoon),전재윤(Chae Yoon Chon),김기황(Ki Whang Kim),박인서(In Suh Park) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.3
N/A Background/Aims: In the evaluation of esophageal symptoms, the endoscopy, conventional esophagography using liquid barium and esophageal manometry have been used for the detection of organic and functional esophageal diseases. But some questions were raised that these studies were not capable of reproducing the esophageal symptoms and the manometry was often not a confirmative test in all functional esophageal diseases. So, we performed an esophagography using the semisolid material, marshmallow, and evaluated its clinical usefulness in patients with func- tional esophageal diseases. Methods: We perfonned the endoscopy, conventional esophagography and marshmallow esophagography in 54 consecutive patients with esophageal symptoms(dysphagia, globus symptom and non-cardiac chest pain) and esophageal manometry in 21 patients among them, and compared with asymptomatic, healthy volunteers(n=9). Results: The marshmallow esophagography revealed abnormal hesitation and impaction in 15 patients(45%) with normal conventional esophagography(n=33) and in 4 patients(57%) with normal esophageal manometry studies(n=7). In contrast with these result, all of the conventional esophagography were normal in patients with normal marshmallow esophagography(n=17). In the reproducibility of symptoms, the dysphagia group was greater than another groups(p(0.05) and there was a trend towards a high reproducibility of symptoms in patients who showed the severe grade(p (0.05). The abnormal marshmallow esophagography was correlated with the abnormal peristalsis of conventional esopha- gography and nonspecific esophageal motility disorder of manometry(p(0.05). Conclusions: We found the marshmallow esophagography was rnore sensitive and capable of reproducing the sypmtoms than conventional liquid barium esophagography in the evaluation of the esophageal symptoms, and the marshmallow esophagography is thought to be valuable as one of diagnostic tools for esophageal motility disorders. (Korean J Gastroenterol 1996; 28:303-310)