Sarcoidosis 보고

서국원 ( Guk Won Suh ),어수택 ( Suh Tak Uh ),안병수 ( Byung Su Ahn ),박춘식 ( Chun Sik Park ),김극배 ( Guk Bae Kim ),이동화 ( Dong Hwa Lee ) 대한결핵 및 호흡기학회 1984 Tuberculosis and Respiratory Diseases Vol.31 No.4

갑상선 기능항진증에서 인슐린 분비 및 적혈구 인슐린 수용체에 관한 연구

나우균(Woo Kyun Na),김극배(Guk Bae Kim),유명희(Myung Hi Yoo),윤석중(Seuk Joong Yeon) 대한내과학회 1987 대한내과학회지 Vol.33 No.3

N/A Impaired glucose tolerance is well recognized in hyperthyroidism. To investigate the mechanism of glucose intolerance in hyperthyroidism, we performed IV GTT and erythrocyte insulin receptor assay in 7 normal control, 16 thyrotoxic patients and 6 euthyroid patients after antithyroid medication. The glucose decay constant K was decreaaed (K<1.0) in 31% (5/16) of thyrotoxic patients. Insulin secretion at the 1st phase during IV GTT was inereased in thyrotoxic patients compared with normal control (Area under the curve, 761±196 vs 322±49 μU/ml min p<0.05), but 2nd phase insulin secretion showed no significant difference. 125 I-insulin max. specific binding percent of the erythrocyte showed no significant difference in throtoxic patients compared with normal control (p>0.05). Thyrotoxic patients were divided into two groups: group I (n=11) who showed impaired glucose tolerance (K>1.0) and group II (n=5) who showed impaired glucose tolerance (K < 1.0) during IV GTT. 1st phase insulin secretion was increased in group I compared with group II patients (AUC 941±251 vs 310±132 μU/ml min, p<0. 05), while 2nd phase insulin release showed no significant difference. 125 I-insulin max. specific binding percent of erythrocytes showed no significant difference in group I compared with group II (9.80±0.75/o vs 10.40±0.77%, p>0.05). In 5 euthyroid patients on antithyroid medication, insulin secretion & erythrocyte insulin receptor assay showed no significant change after treatment. In conclusion, Ist phase insulin secretion was increased and erythrocyte insulin receptor assay was normal in thyrotoxic patients, suggesting that pheripheral postreceptor defect or hepatic insulin resistance may be responsible for the glucose intolerance of hyperthyroidism.

당뇨병환자에서 동맥경화증의 병인에 관여하는 내피세포 및 혈소판의 기능에 관한 연구

우종욱(Chong Wook Woo),김현수(Hyun Soo Kim),홍대식(Dae Sik Hong),박희숙(Hee Sook Park),유명희(Myung Hi Yoo),김극배(Guk Bae Kim) 대한내과학회 1989 대한내과학회지 Vol.37 No.1

N/A Atherosclerosis is well known as a major complication in diabetes. To investigate the role of platelet function, Factor VIII: vWF and prostacyclin in the pathogenesis of atherosclerosis in diabetes, we performed a platelet function test and measured PGF 1 which is a metabolite of prostacylin, Factor VIII: vWF and thromboxande B2 in 15 patients with diabetes and 10 normal controls. The platelet function test showed that ADP-induced platelet aggregation was significantly enhanced in diabetic patients with retinopathy (80.6±11. 45% vs 42.6±8.71%, p<0.05) compared with normal controls, but collagen induced platelet aggregation or platelet adhesiveness showed no significant difference between diabetic patients and normal controls. The plasma Factor VIII R: Ag level in diabetic patients as not signficantly different from normal controls while plasma Factor VIII: vWF in diabetic patients was significantly increased compared with normal controls (51.33±5.33% vs 27.50±6.71%, p<0.05) The prostaglandin F1α level was signficantly decrease in diabetic patients (2.01±0.32 pg/tube vs 3. 17±0.48pg/tube, p<0.05), while the plasma thromboxane B2 level showed no signficant difference between diabetic patients and normal controls. Our data showed that the increase plasma Factor VIII: vWF level and decrease PGF1α level as well as abnormal platelet function may contribute to the mechanism of atherosclerosis in diabetic patients.

간경변증 및 뇌질환 환자의 혈장 APV의 측정과 수분대사에 대한 연구

김극배 순천향대학교 1984 논문집 Vol.7 No.1

In order to studying the water balance in brain disease and liver cirrhosis. 15normal control subjects, 10 patients of liver cirrhosis and 20 patients of brain disease were subjected to determine plasma AVP levels by sensitive radioimmuno assay and simultaneous determined plasma and urine osmolality, Results were. 1. Plasma AVP concentration in raodomly hydrated normal adult control was average 2.0 pg/ml with range of 0.4~5.6 pg/ml. Results was fairly correlated the serum osmolality and osmotic threshold was 280.4 mOsm/kg. 2. Plasma AVP level in 10 patient of liver cirrhosis was average 2.8pg/ml. with range of 0.2~9.0 and plasma and urine osmolality ratio was marked decrease in compare with normal subjects and poorly corrlated with plasma AVP and plasma osmolality. 3. Plasma AVP level in 20 patients of brain disease was average of 0.4~9.0pg/ml. There are no difference of plasma level in average concentration with normal control but marked fluctuation between patients. And also poorly correlated with plasma AVP and plasma osmolality. 4. There are negligible AVP concentration and marked decreased U/P ratio in two cases of polyuric patients. 5. Studies suggested there are more complex osmotic, nonosmotic stimuli and inhlbitory stimuli affected in liver cirrhosis and brain disease.

폐경기전후 한국 여성의 비타민 - D 수용체 유전자의 Restriction Fragment Length Polymorphisms 과 골밀도 및 골표지자와의 관계에 대한 고찰

김극배,한인권,서교일,변동원,김상우,유명희,문인걸 대한내분비학회 1995 Endocrinology and metabolism Vol.10 No.3

Osteoporosis is now a major health problem because of the increasing elderly population and related osteoporosis fractures. Recently, it has been suggested that lower bone mass with/and high bone turnover rate is considered to be important in the developing of osteoporosis, and so there has been many efforts to identify the risk factors which is considered to cause lower bone mass and high bone turnover. Osteocalcin, the most abundant noncollagenous protein in bone, is a marker of bone turnover and its synthesis is induced by calcitriol(the active form of vitamine-D) through the vitamine-D receptor(VDR) and a specific vitamine D-responsive element in the osteocalcin gene promoter. Serum concentrations of osteocalcin are under the strong genetic influences and may reflect allelic variation in VDR gene. Therefore, the present study were designed to find the relationships among the polymorphisms of Vitamine-D receptor gene, bone mineral density and bone markers. We analysed the restriction fragment length polymorphisms of VDR gene with Bsm I endonuclease enzyme in relation to bone mineral density by using DEXA(dual energy X-ray absorptiometry, QDR-2000) and bone markers, especially serum osteocalcin concentrations in 356 pre- and postmenopausal Korean women. The frequence of RFLPs of VDR gene is 3.3% in BB type, 10.1% in Bb type, 86.6% in bb type. The concentrations of osteocalcin, alkaline phosphatase, procollagen-C and urinary deoxypyridinoline/creatinine were found to be higher in postmenopausal than premenopausal women and the levels of BMD were lower in postmenopausal than premenopausal women. The BB type, which is known to have a strong genetic determinant, is less frequently encountered in Korean women and does not correlate with levels of bone markers and bone mineral density. Even though the number of women with BB type is small, we noted the mean serum level of each bone marker was greater in postmenopausal women with BB type than in premenopausal women with the same genotype. In conclusion, this may suggest a partial agreement of our data with that of Australlian group and that we have to try to find out another genotype specifically related with lower bone density in Korean women(J Kor Soc Endocrinol 10: 249-261, 1995)

갑상선질환에서 thyroid stimulating immunoglobulin의 측정

한동철,유명희,김극배 순천향대학교 1985 논문집 Vol.8 No.1

To investigate the role of thyroid stimulating immunoglobulin(TSI) in various thyroid diseases, we measured TSI activity by TSH receptor Ab in 17 patients with Grave's diseases(GD), 4 patients with thyroiditis and 4 patients with thyroid nodules and also measured serum T₃, T₄, TSH, T₃RU by radioimmunoassay. 1) In patients with 14 hyperthyroid GD, the levels of serum T3,T4, were significantly increased than normal control. The levels of serum T3 in patients with thyroiditis and the levels of serum T4 in patients with thyroid nodule was significantly increased than normal control. 2) the levels of TSI in hyperthyroid GD(33.4±27.7%) and euthyroid GD (22.9±18.3%) were significantly increased than normal control. There was no correlation between the levels of TSI and thyroid function tests in patients with GD. 3) Decreased levels of TSI was observed after antithyroid drug theraphy in patients with 4 GD.

High Performance Liquid Chromatography를 이용한 Hemoglobin A1C 측정

변동원,서교일,유명희,김극배 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

Hemoglobin AIc(HbAlc) has been known to be an index of long-term glycemic control (6-8weeks), and offers several practical advantages for screening of diabetes mellitus and evaluation of effects of treatment. Authors introduced to measure and investigate HbAlc levels by high performance liquid chromatoraphy (HPLC ; LDC Milton Roy, USA), with cation exchange column, Mono S HR 5/5 (Pharmacia). The results were as follows; 1. The mean HbAlc level in normal adult control group (n=50) was 4.13±0.25% (Mean±S.D). 2. Within-run precisions of HbAlc levels measured by HPLC were 4.39±0.01% (Mean±S.D.), 1.41(C.V.) in normal control, and 8.95±0.02% (Mean±S.D.), 1.65(C.V.) in intermittently treated diabetic patients, and 11.02±0.01% (Mean±S.D.) 2.93(C.V.) in untreated diabetic patients. 3. Between-run precision of HbAlc levels measured by HPLC were 4.35±0.01% (Mean±S.D.), 2.76(C.V.) in normal control, and 8.98±0.01% (Mean±S.D.), 1.22(C.V.) in intermittently treated diabetic patients, and 10.95±0.15% (Mean±S.D.), 3.51(C.V.) in untreated patients. 4. The peak level of HbAlc was obtained in about 9 minutes and the whole program could be completed within 17 minutes. 5. Blood specimens could be stored for as long as 8 days at 4℃ without changes of HbAlc levels. These results suggest that the measurement of HbAlc by HPLC using cation exchange column is so accurate, simple and rapid that the clinical use is more wider.

당뇨병 환자에서 임상증상 및 자율신경장애와 감각신경장애와의 상관관계

김진오,유용규,문승혁,김효석,봉형근,변동원,서교일,조용욱,유명희,김극배 순천향대학교 1994 논문집 Vol.17 No.2

In a group of 47 diabetic patients and 60 controls, we measured vibration perception threshold with biothesiometer, and autonomic nerve function by means of the five classical cardiovascular tests (Valsalva ratio, R-R interval variations during deep breathing, sustained handgrip, lying to standing, and postural hypotension). Results of cardiovascular reflex results were compared with symptoms of autonomic dysfunction and vibration perception threshold. The object of this study was to assess the physiological and clinical correlation of autonomic and sensory neuropathy in diabetic patients and results of this study could be applying patients for therapeutic approach and follow up diabetic neuropathy. Vibration perception threshold correlated with Valsalva ratio(p<0.01) and deep breathing(p<0.01). Patients score was significantly lower than controls in vibration perception threshold and all of the autonomic function tests. According to the results of cardiovascular tests, patients were divided into two different groups : presence(DAN+: ≥3) or abscence(DAN-: <3) of autonomic neuropathy. DAN (­) group(n=25, 53.2%) showed no significant differences from the DAN(+) group(n=22, 46.8%) in age, fasting and 2 hours postprandial blood sugar, body mass index, and cholesterol but associated with duration of diabetes. Vibration perception threshold measured at the first toe tip and at external malleolus in DAN(+) patients were significantly higher than DAN (­) patients and controls. We diagnosed sensory impairment when vibration perception threshold value is above 95th percentile of controls and we observed a significant correlation between autonomic and sensory neuropathy. These findings suggested that there is a correlation between autonomic and sensory neuropathy in diabetic patients, even in the absence of symptoms.

정상인에서 베타3-아드레날린 수용체 유전자의 변이가 복부 비만도 및 지질대사에 미치는 영향

김영선,윤석기,김철희,서교일,김학선,김극배,변동원,유명희 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

The β₃-adrenergic receptor is expressed in visceral adipose tissue in humans and is thought to contribute to the regulation of the resting metabolic rate and lipolysis. We studied the influence of a mutation in the β₃ -adrenergic receptor gene(Trp64Arg) on body fat distribution, central obesity, lipid metabolism in 65 healthy young male adults. One out of 65 subjects were homozygous (Arg/Arg) for the trp64Arg mutation, 17 subjects were heterozygous (Trp/Arg), and 47 lacked the mutation(Trp/Trp). The body weight, height, body mass index, waist-to-hip ratio were similar between the subjects with- and without the mutation. Total body fat, abdominal fat amount, body fat distribution, serum total- and HDL-cholesterol, triglyceride, glucose, and insulin concentrations were also not different according to the presence or absence of the mutation. These results suggested that Trp64Arg mutation in β₃-adrenergic receptor gene is not a major contributing factor for central obesity or change in lipid metabolism in Korean young adults.

정상상태의 지질대사

김극배 대한당뇨병학회 1985 Diabetes and Metabolism Journal Vol.9 No.2