실시간 광단층 모니터링 안구 수술용 현미경 프로브 개발

김경운,이창호,정효상,한승훈,김홍균,김지현,Kim, Kyung-Un,Lee, Chang-Ho,Jeong, Hyo-Sang,Han, Seung-Hoon,Kim, Hong-Kyun,Kim, Jee-Hyun 대한의용생체공학회 2012 의공학회지 Vol.33 No.2

Intraoperative surgical microscope is an essential surgical equipment. However, it has a restriction to classify the retina layers because of the contrast differences. To solve this problem, operators use surgical instrument such as an intraocular mirror. In this case, it has to amputate the patient's eye. In this study, we developed a probe the intraoperative surgical optical coherence tomography. We expect that the developed OCT probe can overcome the limit of OCT and be applied as a real-time surgical tool. In this paper, we demonstrate applicability of the probe through rabbit's experimentation.

말기암환자가 보완대체요법을 선택한 이유

김경운,박진노,이수한,Kim, Kyeong-Uoon,Park, Jean-No,Lee, Soo-Han 한국호스피스완화의료학회 2011 한국호스피스.완화의료학회지 Vol.14 No.1

목적: 말기암환자들이 보완대체요법을 선택하게된 주관적 경험을 파악하여, 대상자 입장에서의 당위성을 이해하고 이용과정에서 발생할 수 있는 현상들을 이해함으로써 좀 더 바람직하고 효율적인 이용을 위한 호스피스 완화의료 중재를 하는데 기여하고자 실시하였다. 방법: 심층면담한 내용을 Colaizzi(1976)의 방법을 이용하여 귀납적이고 서술적인 기술과 분석을 실시하였다. 결과: 말기암환자와 가족들이 보완대체요법을 선택한 4가지 범주는 첫째 현대의학의 한계 인식, 둘째 보완대체요법의 효과에 대한 신뢰, 셋째 가족의 정서적 욕구 충족 넷째, 의사의 부정적인 태도로 인한 불신이었다. 주제모음은 9가지로 '불가능한 완치에 대한 기대', '병원치료에 대한 불확실성', '항암치료의 부작용을 해결하기 위한 보조적 수단', '증상완화와 생명연장을 위함', '암치료의 부작용에 대한 두려움', '체험과 정보에 대한 신뢰', '타인의 권유', '가족으로서 도리를 다함', '의사의 부정적 태도에 불만족'이었다. 결론: 암환자의 치료 결정과정에서 대상자와 의사가 바람직한 의사소통과정을 통하여 현대의학 치료에 대한 설명이 충분히 이루어져서 대상자가 일반적인 호스피스 완화의료 서비스를 이용하고 과학적인 근거에 바탕을 둔 보완대체요법에 대한 정확한 정보 제공이 필요하다고 본다. Purpose: The purpose of this study was to analyze the reason to select complementary and alternative therapy for terminally ill cancer patients. Methods: The data were collected from 21 terminal cancer patients and families through the in-depth interview. Data analysis were performed by the Colaizzi's phenomenological method (1976). Results: The reason to select complementary and alternative therapy for terminal cancer patients and families was then categorized with 4 elements; Awareness of limitations in contemporary medical treatments, Belief in effectiveness of the CAM, Satisfaction with emotional needs of family members, and Disbelief due to negative attitudes of physicians. The result indicated the following 9 themes expectation for a complete cure, uncertainty in hospital treatments, complementary method for management of side effect of chemotherapy, alleviation of symptoms and life-sustaining, fear for side effects of cancer treatments, belief in earned information, referrals by other, responsibility of family, and dissatisfaction with negatine attitudes of physicians. Conclusion: Physicians should provide a sufficient explanation and try to effectively communicate with clients about hospice and palliative service and the CAM. We strongly realized that concerns about patients' best care and satisfactions with family's needs should be understood.

봉약침과 홍화약침을 이용한 원형탈모증(圓形脫毛症) 치험 1예

김경운,이경민,임성철,정태영,한상원,서정철,Kim, Kyung-Woon,Lee, Kyung-Min,Lim, Seong-Chul,Jung, Tae-Young,Han, Sang-Won,Seo, Jung-Chul 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.2

Objective : Alopecia areata is a common disease and the difficulty of its medical management is well known. This study was designed to investigate the effect of bee venom and Carthami Flos herbal acupuncture. Method : The patient was managed by bee venom, carthami-flos herbal acupuncture and General acupunture. Herbal acupuncture was injected subcutaneously into the lesion. We checked involvement in plaque, according to evaluation of the effect on alopecia areata. Results : After 5 month of treatment, the lesion had been replaced with new terminal hair. Evaluation of the effect on alopecia areata The score changed from 0 to 3 point. Conclusion : These result suggest that bee venom and Carthami Flos herbal acupuncture has good effect on alopecia areata. But further studies are required to concretely prove the effectiveness of bee venom and Carthmi Flos herbal acupuncture for treating alopecia areata.

류마티스 관절염 환자의 말초혈액 단핵세포에서 Phosphoinositide 3-Kinase (PI3K)/Akt와 Nuclear Factor KappaB (NF-κB) 신호전달을 통한 IL-17 생성조절

김경운,조미라,이상헌,민소연,박미경,박성환,주대명,김호연,Kim, Kyoung-Woon,Cho, Mi-La,Lee, Sang-Heon,Min, So-Youn,Park, Mi Kyung,Park, Sung-Hwan,Jue, Dae-Myung,Kim, Ho-Youn 대한면역학회 2003 Immune Network Vol.3 No.4

Inflammatory mediators has been recognized as an important role in the pathogenesis of rheumatoid arthritis (RA). IL-17 is increasingly recognized as an important regulator of immune and inflammatory responses, including induction of proinflammatory cytokines and osteoclastic bone resorption. Evidence of the expression and proinflammatory activity of IL-17 has been demonstrated in RA synovium and in animal models of RA. However, the signaling pathways that regulate IL-17 production remain unknown. In the present study, we investigated the role of the phosphatidylinositol 3 kinase (PI3K)-Akt pathway in the regulation of IL-17 production in RA. PBMC were separated from RA (n=24) patients, and stimulated with various agents (anti CD3, anti CD28, PHA, ConA, IL-15). IL-17 levels were determined by sandwich ELISA and RT-PCR. The production of IL-17 was significantly increased in cells treated with anti-CD3 antibody, PHA, IL-15 or MCP-1 (P<0.05). ConA also strongly induced IL-17 production (P<0.001), whereas TNF-alpha, IL-1beta, IL-18 or TGF-beta did not. IL-17 was detected in the PBMC of patients with osteoarthritis (OA) but their expression levels were much lower than those of RA PBMC. Anti-CD3 antibody activated the PI3K-Akt pathway and activation of the PI3K-Akt pathway resulted in a pronounced augmentation of nuclear factor kappaB ($NF-{\kappa}B$). IL-17 production by activated PBMC in RA is completely or partially blocked in the presence of $NF-{\kappa}B$ inhibitor PDTC and PI3K-Akt inhibitor, wortmannin and LY294002, respectively. Whereas the inhibition of AP-1 and extracellular signal-regulated kinase (ERK)1/2 did not affect IL-17 production. These results provide new insight into that PI3K/Akt and $NF-{\kappa}B$ dependent signal transduction pathway could be involved in the overproduction of key inflammatory cytokine, IL-17 in rheumatoid arthritis.

류마티스 관절염에 있어 종양괴사인자 다형성에 대한 연구

김경운,이경민,이봉효,임성철,정태영,서정철,Kim, Kyung-Un,Lee, Kyung-Min,Lee, Bong-Hyo,Lim, Seong-Chul,Jung, Tae-Young,Seo, Jung-Chul 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : Tumor necrosis factor-${\alpha}{\cdot}$(TNF-${\alpha}{\cdot}$) is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. This study was designed to investigate the relation between TNF-${\alpha}{\cdot}$ gene polymorphism and rheumatoid arthritis in Korean population. Methods : This study was carried out on 103 rheumatoid arthritis patients who fulfilled the American College of Rheumatology 1987 revised criteria for rheumatoid arthritis and 208 healthy control subjects. Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerse chain reaction(PCR). PCR products were visualized by 2% agarose gel electrophoresis. We investigated the genotyping of TNF-${\alpha}{\cdot}$ by using Pyrosequencing. Results : The genotypes of TNF-${\alpha}{\cdot}$ gene were GG, AG and AA. While the distribution of TNF-${\alpha}{\cdot}$ polymorphism in control subjects was 92.31%, 7.21%, 0.48% respectively, in rheumatoid arthritis patients was 93.20%, 6.80%, 0.00%(GG, AG. AA). There was no statistical significant allelic frequency difference between control and rheumatoid arthritis groups. Conclusion : We concluded that there was no significant association between TNF-${\alpha}{\cdot}$ gene polymorphism and rheumatoid arthritis. However, the findings of this study need to be confirmed in more patients and further studies.

B cell-associated immune profiles in patients with end-stage renal disease (ESRD)

김경운,정병하,Eun Joo Jeon,Bo-Mi Kim,최범순,박철휘,김영수,조석구,조미라,양철우 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.8

Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused on T cell immunity. We investigated B cell subpopulations in ESRD patients and the effect of hemodialysis (HD)on B cell-associated immune profiles in these patients. Forty-four ESRD [maintenance HD patients (n = 27)and pre-dialysis patients (n = 17)] and 27 healthy volunteers were included in this study. We determined the percentage of B cell subtypes, such as mature and immature B cells, memory B cells, and interleukin (IL)-10+cells, as well as B cell-producing cytokines (IL-10, IL-4and IL-21) by florescent activated cell sorting (FACS). B cell-associated gene expression was examined using real-time PCR and B cell producing cytokines (IL-10, IL-4 and IL-21) were determined using an enzyme-linked immunosorbent assay (ELISA). The percentage of total B cells and mature B cells did not differ significantly among the three groups. The percentages of memory B cells were significantly higher in the pre-dialysis group than in the HD group (P < 0.01), but the percentage of immature B cells was significantly lower in the pre-dialysis group than in the other groups. The percentages of IL-10-expressing cells that were CD19+ or immature B cells did not differ significantly (P > 0.05) between the two subgroups within the ESRD group, but the serum IL-10 concentration was significantly lower in the pre-dialysis group (P <0.01). The results of this study demonstrate significantly altered B cell-associated immunity. Specifically, an imbalance of immature and memory B cells in ESRD patients was observed, with this finding predominating in pre-dialysis patients.

한국어판 McMaster Quality of Life Scale를 이용한 호스피스.완화의료 환자의 삶의 질

김경운,Kim, Kyeong-Uoon 한국호스피스완화의료학회 2010 한국호스피스.완화의료학회지 Vol.13 No.4

Purpose: This study was to measure quality of life in hospice and palliative care patients. Methods: Research tools were the Korean version of McMaster quality of life scale and Spitzer's quality of life index used by 11 doctors in 7 institutes related to hospice and palliative care. Research subjects were 70 terminal cancer patients. Results: There was no statistically significant differences in the Korean version MQLS score by test-retest method. Cronbach's alpha was 0.90. The Pearson correlation coefficient for concurrent validity between the Korean version MQLS score and QLI score by physicians showed a moderate level of statistically significant correlation (r=0.55, P=0.002). The Korean version MQLS score of terminal cancer patients group in hospital ward of hospice was significantly higher than the others (F=4.958, P=0.004). Total scores of the Korean version MQLS ($Mean{\pm}SD=3.79{\pm}0.74$) and QLI ($Mean{\pm}SD=4.58{\pm}1.63$) showed moderate scores. Conclusion: The Korean version MQLS would be a reliable methods for the measurement of quality of life in Korean palliative care patients. The research tool would be satisfactorily used for assessment of hospice and palliative care patients during palliative phase. 목적: 본 연구의 목적은 호스피스 완화의료 대상자의 삶의 질을 측정하기 위하여 실시되었다. 방법: 연구도구는 한국어판 McMaster Quality of Life Scale과 Spitzer's Quality of Life Index이고 7개 호스피스 완화의료기관의 의사 11명이 사용하였다. 연구대상자는 70명의 호스피스 완화의료 환자들이었다. 결과: 첫째, 검사-재검사법에 의한 한국판 MQLS 점수는 통계적으로 유의한 차이가 없었다. Cronbach's alpha 는 0.90이었다. 의사들에 의해 측정된 한국어판 MQLS와 QLI의 동시타당도를 위한 Pearson correlation coefficient는 중등도의 통계적으로 유의한 상관관계를 보였다(r=0.55, P=0.002). 독립형 호스피스기관의 말기암환자군의 한국어판 MQLS 삶의 질 점수가 다른 군들보다 유의하게 높았다(F=4.958, P=0.004). 한국어판 MQLS 전체점수($Mean{\pm}SD=3.79{\pm}0.74$)와 QLI 전체 점수($Mean{\pm}SD=4.58{\pm}1.63$)는 중등도의 삶의 질 점수임을 알 수 있었다. 결론: 상기의 연구결과로 한국어판 MQLS 도구는 호스피스 완화의료 환자에게 삶의 질을 측정하는데 신뢰할만한 도구로써 입증되었다. 이런 도구들은 완화적인 시기동안 호스피스 완화의료 환자의 사정을 위해서 활용될 수 있다.

색순도가 우수한 갈륨 산화물계 적색 형광체의 광발광 특성

김경운,최성호,정하균,남산,Kim, Kyoung-Un,Choi, Sung-Ho,Jung, Ha-Kyun,Nahm, Sahn 한국재료학회 2008 한국재료학회지 Vol.18 No.3

$Eu^{3+}$-activated $R_3GaO_6$ (R = Y, Gd) phosphors were prepared in a conventional solid-state reaction and their optical properties were investigated. These compounds exhibit strong red emission under light excitation at 254 nm. The emission spectra are dominated by peaks appearing around 610-630 nm that are induced by the electric dipole transition of $^5D_0\;{\rightarrow}\;^7F_2$ of $Eu^{3+}$. In addition, the appropriate CIE (Commission Internationale de l'clairage) chromaticity coordinates, (x = 0.656, y = 0.336) for $Y_3GaO_6$ and (x = 0.655, y = 0.334) for $Gd_3GaO_6$, become closer to the NTSC (National Television System Committee) standard values. With the optimized activator concentrations, the maximum emission brightness is approximately 80% of $Y_2O_3$:$Eu^{3+}$ typical red-emitting phosphor with improved color purity under an excitation condition of 254 nm.

류마티스관절염 활막세포에서 IL-17과 TLR 상호전달을 통한 RANKL과 IL-6 생성 조절

김경운 ( Kyoung Woon Kim ),이상헌 ( Sang Heon Lee ),조미라 ( Mi La Cho ),오혜좌 ( Hye Joa Oh ),우윤주 ( Yun Ju Woo ),김석형 ( Suk Hyung Kim ),김해림 ( Hae Rim Kim ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.1

Objective: The aim of this study was to clarify whether stimulation of recombinant IL-17, TLR2 and TLR4 by their specific ligands induces the production of RANKL and IL-6 in the fibroblast-like synoviocytes (FLSs) from RA patients. Methods: FLSs were isolated from RA synovial tissues and they were stimulated with the IL-17, TLR2 ligand bacterial peptidoglycan (PGN) and TLR4 ligand lipopolysaccharide (LPS). The RANKL levels were assessed by RT-PCR and western blotting. The expressions of IL-17, TLR2, TLR4, RANKL and IL-6 in the RA synovium were quantified by immunohistochemistry and these values were compared with the values obtained in the osteoarthritis synovium. The increased IL-6 production in the culture supernatants of the RA FLSs was quantified by sandwich ELISA. Results: The mRNA and protein levels of RANKL and IL-6 increased in the RA FLSs stimulated with PGN, LPS and IL-17, or PGN plus IL-17 or LPS plus IL-17. The expressions of IL-17, TLR2, TLR4, RANKL and IL-6 were much higher in the RA synovium than those in the osteoarthritis (OA) synovium. Conclusion: We observed synergistic effects of TLR-2, TLR-4 and IL-17 upon the induction of RANKL. In conclusion, our data supports the previous evidence of an important role of TLR-2, TLR-4 and IL-17 in the pathogenesis of RA.

수복형 도시정비 사업을 통한 물리적 환경변화에 대한 대응방식 - 국내외 사례를 대상으로 -

김경운(Kim, Kyung-Wun),김수미(Kim, Soomi) 대한건축학회 2022 대한건축학회 학술발표대회 논문집 Vol.42 No.1

In the past, the direction of urban development took the form of large-scale demolition, but the limitations of large-scale redevelopment have become evident. Urban renewal projects since the 2000s have changed to small-scale urban development. However, design guidline. and stable standards for small-scale projects have not yet been achieved. Accordingly, this study analyzed urban redevelopment project cases and examined the differences according to changes in infrastructure and industrial facilities. Based on this analysis, we propose changes in industry and infrastructure for more effective urban development.