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보문 : Lipopolysaccharide에 의해 유도된 RAW 264.7 cell에서의 견우자(Pharbitis nil Choisy) 추출물의 염증억제 효과
고민정 ( Min Jung Ko ),박상용 ( Sang Yong Park ),이강진 ( Kang Jin Lee ),이태후 ( Tae Hoo Yi ),이우철 ( Woo Cheol Lee ),한창균 ( Chang Kyun Han ),길재호 ( Jea Ho Khil ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.2
This study was investigated to find a candidate which has an anti-inflammatory activity on RAW 264.7 cells. It was performed by NO assay, RT-PCR and western blot analysis. In the course of screening, it was found that extracts of Pharbitis nil Choisy showed strong activity of anti-inflammation. Therefore, extracts of Pharbitis nil Choisy was sequentially fractionated with hexane, m ethylene chloride, ethyl-acetate, butanol and water. Of the sequential solvent fractions, the ethylacetate fraction inhibited the mRNA and protein expression of LPS-induced iNOS and COX-2. Taken together, these results suggest that extracts of Pharbitis nil Choisy have significant effects on inflammatory factors, and may be a potential antiinflammatory therapeutic plant.