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Downregulation of SGK1 Expression is Critical for TGF-β-induced Apoptosis in Mouse Hepatocytes Cells
In-Koo Nam(남인구),Jiyun Yoo(유지윤) 한국생명과학회 2012 생명과학회지 Vol.22 No.11
Transforming growth factor-β (TGF-β)에 의해 유도되는 세포사멸 과정은 간에서 손상 받은 조직이나 비정상적인 조직을 제거하는데 중요한 역할을 담당한다. 간세포에서 TGF-β에 의해 유도되는 세포사멸 과정 동안 중요한 기능을 담당하는 유전자를 탐색하고자 쥐의 간세포인 AML12 세포를 이용하여 TGF-β 처리 전후에 발현이 변화되는 유전자를 microarray analysis를 통해 확인하였다. TGF-β에 의해 여러 가지 유전자들의 발현이 변화됨을 확인하였는데, 그 가운데 여러 가지 세포사멸 인자들의 활성을 억제하여 세포사멸을 억제한다고 알려져 있는 SGK1의 발현 감소를 확인하였다. TGF-β에 의해 SGK1의 mRNA와 단백질 level이 모두 감소함을 확인하였고, 항상 active한 형태의 SGK1 (CA-SGK1)을 발현시켰을 때 TGF-β에 의한 세포사멸이 억제됨을 확인하였다. 이러한 결과들은 간세포에서 SGK1의 발현 감소가 TGF-β에 의한 세포사멸을 유도하는데 중요한 기능을 담당할 가능성이 높음을 의미하는 것이다. Transforming growth factor (TGF)-β-dependent apoptosis is important in the elimination of damaged or abnormal cells from normal tissues, especially in liver, in vivo. To investigate which gene expressions are critical for TGF-β-induced apoptosis in hepatocytes, gene expression profiling experiments were performed with TGF-β-treated and non-treated mouse hepatocytes AML12 cells. Findings showed that serum and glucocorticoid-inducible protein kinase1 (SGK1) expression is markedly downregulated during TGF-β-induced apoptosis. Findings confirmed that expression of SGK1 protein, as well as mRNA, is also markedly decreased with TGF-β treatment. Infection of adenoviral vector encoding constitutively active SGK1 (CA-SGK1), but not kinase dead SGK1 (KD-SGK1), attenuated TGF-β-induced apoptosis. All of these results suggest that downregulation of SGK1 expression is critical for TGF-β-induced apoptosis in AML12 cells.
구남인,송영근,윤경규,이경철,Koo, Nam In,Song, Yeong Geun,Yoon, Kyeong Kyu,Lee, Kyeong Cheol 국립한국농수산대학교 교육개발센터 2022 현장농업연구지 = Journal of practical agricultural resear Vol.24 No.2
Prunus × yedoensis is a representative tree species in residential areas often used as a street tree or park tree, with its popularity due to splendid flowers in the spring. We investigated plant growth, photosynthesis, chlorophyll, and carotenoid concentrations of Prunus yedoensis at 400ml/pot, 1,000ml/pot, and 2,000ml/pot Irrigation treatments. Overall growth indices such as tree height, diameter at root collar (DRC), and total dry weight could be ranked in the decreasing order "2,000ml/pot > 1,000ml/pot > 400ml/pot.". However, 2,000ml/pot featured decreased photosynthesis and total chlorophyll carotenoid contents than 1,000ml/pot treatment after July. And also, The 1,000ml/pot treatment showed relatively high photosynthetic activity and chlorophyll contents. These results suggested that the cultivation of Prunus yedoensis under 1,000ml/pot Irrigation treatment(soil water contents 25~35%) showed the best condition in growth.
광산 인근 밭토양에서 중금속 안정화제 처리 및 복토층 처리에 의한 토양 중 중금속 식물유효도 변화 및 고추의 중금속 흡수
김권래(Kwon-Rae Kim),박정식(Jeong-Sik Park),김민석(Min-Suk Kim),구남인(Nam-In Koo),이상환(Sang-Hwan Lee),이진수(Jin-Su Lee),김성철(Sung-Chul Kim),양재의(Jae-E Yang),김정규(Jeong-Gyu Kim) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.6
At the current situation of continuous utilization of heavy metal contaminated upland for agricultural purpose in Korea, minimizing transport of heavy metals from soil to crops is important for securing safety of human health. The present study (in field scale) examined the efficiency of several soil amendments (dolomite, steel slag, lime, zeolite, and compost) on reduction of phytoavailable heavy metals (Cd, Cu, Pb, and Zn) in soil through comparison with no amendment treatment and clean soil cover treatment. For determination of the phytoavailability, 1 M NH4NO3 extraction and red pepper cultivation were introduced. Among the amendments, in general, dolomite and steel slag were the most effective in reduction of metal (Cd, Pb, and Zn) phytoavailability resulting in less accumulation of these metals in shoot and fruit of red pepper. However, dolomite and steel slag treatment was not as effective as clean soil cover treatment which showed the least metal accumulation in red pepper fruit. Nevertheless, with taking into account the cost, treatment of dolomite or steel slag can be competitive method because the current study showed that dolomite or steel slag treatment reduced accumulated heavy metal concentration effectively in both shoot and fruit of red pepper compared to those from control soil and the concentration in fruit was within the standard value (<0.2 mg kg<SUP>-1</SUP> for both Cd and Pb).