雄性不稔을 利用한 水稻 雜種品種 開發 ⅩⅠ. japonica형 水稻에서 育成된 細胞質的-遺傳子的 雄性不稔系統들과 稔性回復系統들의 組合能力

Hee Jong Koh(高熙宗),Mun Hue Heu(許文會) 한국육종학회 1990 한국육종학회지 Vol.22 No.4

We have bred a hybrid rice system of Japonica rice. Combining ability of the developed CGMS and restorer lines, and the extent of heterosis for yield and its associated characters were examined in this study. Rough rice yield of 42 F₁ hybrids ranged from 442.4kg/10a (Gwanak ms/TP1278) to 831. 0kg/10a (Samnam ms/TP1450) with the average of 629.0kg/10a. The heterobeltiosis for grain yield ranged from -31.9% to 15.4% with the average of -1.6%. Estimates of variance for GCA were greater than those for SCA except grain yield, and variances for GCA in all characters except 1000 grains weight were greater in the female (CGMS line) than in the male(restorer line). Combining ability test resulted that Samnam ms as CGMS line, and TP1450, TP664 and TP 1278 as restorer line were promising for hybrid rice. There were significant correlations between the heterosis for grain yield and that for total-shoot dry weight at harvesting stage, and that for harvest index. Grains per panicle out of yield components was mainly responsible for the grain yield of F₁ hybrids. The values of grain yield and its associated characters of F₁ hybrids of CGMS/restorer were generally lower than those of corresponding maintainer/restorer, however there was a highly significant correlation (r=0.8635**) between the grain yield of F₁ of CGMS/restorer and maintainer/restorer. Relative grain yield of F₂ and F₃, in per cent of grain yield of corresponding F₁, ranged from 81.7% to 90.7% and from 87.8% to 97.8%, respectively. None of 14 bulk F₂ and F₃ hybrids, excepts F₃ of Samnam ms/TP929, showed higher yield than its better parent.

작물 분자표지육종의 현황과 전망

고희종(Hee-Jong Koh) 한국원예학회 2011 한국원예학회 학술발표요지 Vol.2011 No.10

Plant breeding strategies for modern digital agriculture

고희종(Hee-Jong Koh) 한국육종학회 2021 한국육종학회 공동학술발표집 Vol.2021 No.-

突然變異로 誘起된 벼의 새로운 優性短稈遺傳子

Hee Jong Koh(高熙宗),Mun Hue Heu(許文會) 한국육종학회 1994 한국육종학회지 Vol.26 No.4

A dwarf line, “Dwarf Hwacheong”, was induced from a japonica cultivar ‘Hwacheongbyeo’ by mutation in rice. Its agronomic characteristics, inheritance of dwarfness, and its response to exogenous gibberellin were studied. The dwarfness of the line was controlled by a single dominant gene, which was different from “D-53”, the only dominant dwarf-gene so far reported. The new gene was designated as “D-h”. The “D-h” gene was found to have the pleiotropic effect on short panicle and small-round grain. The dwarf line showed the similar response to GA₃ and GA₄₊₇ treatment with the original cultivar

벼 雄性不稔系統의 葯 採取時期 및 不稔細胞質이 葯培養에 미치는 影響

Hee Jong Koh(高熙宗),Young Jae Won(元容在),Geon Wan Cha(車建玩),Mun Hue Heu(許文會) 한국육종학회 1995 한국육종학회지 Vol.27 No.2

Anther culturability of CGMS(Cytoplasmic and genetic male-sterile) lines was studied in relation to the pollen-development stage and the effect of sterile cytoplasms in Japonica rice. Callus induction rates of CGMS lines were 45.2%~61.8% in COA-ms and 4.1%~8.1% in WA-ms. Regeneration frequencies ranged from 20.2% to 27.7% without any particula difference between two different cytoplasmic male-sterile lines. Gwanakbyeo and Gwanak COA-ms showed the same callus induction ability along evry pollen-development stages. Callus induction rate of Gwanak WA-ms anther in early uninucleate stage was similiar to that of Gwanakbyeo, however, that was drastically decreased after the stage. It was discussed that sterile pollens caused by sterile cytoplasms were different from S- or E-pollens occuring in normal plants in response to anther culture. Sterile cytoplasms of COA-ms and WA-ms did not affect directly the callus induction and plant regeneration. The regenerated plants derived from anther culture of MS lines were all male-sterile.

우리나라 작물육종 성과와 발전 방안

고희종(Hee-Jong Koh) 한국육종학회 2020 한국육종학회지 Vol.52 No.S

Crop breeding is the genetic improvement of crops for human benefit. In Korea, crop breeding by hybridization was initiated in the early 1900s. Since the development of the first rice variety ‘Namseon 1’ in 1932, a series of great achievements have been made in most of the crops. Of these, the development of ‘Tongil’ rice, which brought self-sufficiency of the staple food due to its high productivity, was a monumental achievement in Korea’s crop breeding history. Laws for Variety Protection and Seed Industry were established as of June 2013. Until 2018, a total of 7,644 crop varieties were registered for protection in the national variety list that is managed by the Korea Seed and Variety Service. Some Research/Development (R/D) programs for promoting crop breeding studies have been implemented with governmental support. However, domestic seed markets have stagnated for years because of reduced cropping area. Considering the fact that international seed market size has increased at an annual rate of more than 7%, seed export is regarded as a breakthrough measure for the expansion of the Korean seed industry. In order to obtain a competitive power in international seed markets, the Korean seed industry sector should be reinforced with manpower training, R/D investment, an international marketing system, and governmental support. More details are discussed.

디지털 기반 식물 육종 현황과 전망

고희종(Hee-Jong Koh) 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

식물 육종은 증가하는 인구를 부양하기 위하여 우수한 품종을 개발함으로써 작물의 생산성을 꾸준히 증가시켜 왔다. 최근의 기후변화, 자원고갈, 병해충의 창궐 등 지구적 환경변화와 시장 및 소비자 요구도의 다변화 등으로 식물 육종의 책임과 역할은 점증하고 있다. 또한 팽창하는 세계 종자산업과 그린바이오 산업을 주도하기 위해 주요 선진국과 주요 기업들은 우수종자 개발에 매진하고 있는 바, 우리나라도 그 대열에 합류해야 한다. 생명공학과 유전체학, 표현체학 등 첨단 기술의 급속한 발전은 식물 육종의 새 패러다임을 열고 있다. 21세기 육종가들은 유전자원 간 교배하는 외에 목표 유전자를 직접 교정하고, 형질전환 기술로 유성생식의 범주를 초월하여 유전자원의 한계를 무한정 확대하는 변이를 창출하고 있다. 잡종 후대 집단 또는 계통에서의 선발은 표현형 선발만이 아니라 유전자를 모니터링하고 유전체 정보를 이용하여 선발함으로써 신속 정확하게 원하는 유전자형을 고정할 수 있다. 최근에는 감수분열과 유전자 재조합에 대한 이해가 깊어짐으로써, 원하는 유전자의 조합을 자유롭게 만들고 목표 유전자형을 작성해 갈 수 있는 길이 열리고 있어서 한 단계 재도약이 기대된다. 이러한 미래 육종기술들을 실현하기 위해서는 방대한 유전자, 유전체, 표현형, 환경데이터들을 디지털 기술 기반 위에서 통합하여 다루어야 한다. 첨단 디지털 기반 육종기술이 상용되기 위해서는 1) 작물별로 형질들에 대한 관여 유전자와 유전체 정보가 구명되어야 한다. 2) 유전체 정보와 표현체 정보 등이 육종가들 사이에 공유되는 것이 바람직하다. 3) 식물 육종을 위한 생명공학 규제 관련 법률들이 현대의 첨단기술을 적극 수용할 수 있어야 한다. 본 발표에서는 디지털 기반 식물 육종과 관련된 기술개발 현황과 과제 등을 논의하고자 한다.

한국에서의 식물 지식재산 침해 예방 및 분쟁해결 방안

고희종(Hee-Jong Koh) 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.10

벼 分蘖開度의 遺傳

고희종(Hee Jong Koh),원용재(Yong Jae Won),차건완(Kean Wan Cha),이종철(Jong Cheol Lee),박순직(Sun Zik Park),허문회(Mun Hue Heu) 한국육종학회 1997 한국육종학회지 Vol.29 No.4

This study was carried out to investigate the inheritance mode of tillering angle using wx126-based near isogenic lines of different tillering angle, Broom, Open and Spread type, and the tillering angle of 73 rice varieties from Korea and IRRI was also estimated. The average tillering angle of rice varieties from Korea and IRRI were 10.5˚ (wide side) and 6.7˚ (narrow side) in japonicas, 12.3˚ (wide side) and 8.3˚ (narrow side) in Tongil type, and 8.7˚ (wide side) and 5.9˚ (narrow side) in Indicas. Average tillering angle at wide side of wx126 (original variety), Broom, Open, and Spread type at heading stage were 8.1˚, 5.5˚, 14.8˚, and 33.9˚, respectively, and the difference of tillering angle among the lines was maintained throughout the whole growth stage. In six F₂ populations of all-possible crosses among three near-isogenic lines, tillering angle showed continuous variation skewed to narrower parent, implying that it should be a quantitative character and the genes conferring the narrower tillering angle might be dominant over those conferring the wider tillering angle. Broad sense heritabilities for tillering angle varied 48.8% to 76.2%.

雄性不稔을 利用한 水稻 雜種品種 開發 Ⅶ. japonica型 水稻의 雜種品種에서 COA-ms를 利用한 稔性回復遺傳子의 連關分析

Hee Jong Koh(高熙宗),Mun Hue Heu(許文會) 한국육종학회 1990 한국육종학회지 Vol.22 No.2

Linkage relationship of restorer gene with marker genes was analyzed in F₂ populations of the crosses between newly developed CGMS linkage testers and restorer line, TP664. The restorer gene had been found to be gametophytic in the earlier report, expected that male-sterile plants did not come out even in F₂ generation so far. Pollen fertility and expression of marker genes in each F₂ plant were examined. The result indicated that the restorer gene was linked with ‘pgl’ gene of ‘fl’ linkage group with the crossovervalue 16-18%.