초임계이산화탄소에 의한 톳과 알로에 추출물의 수율 및 항균활성

임상빈(Sang-bin Lim),김수현(Soo-Hyun Kim),고영환(Young-Hwan Ko),오창경(Chang-Kyung Oh),오명철(Myeong-Cheol Oh),고용구(Yong-Gu Ko),박제석(Che-Seok Park) 한국식품과학회 1995 한국식품과학회지 Vol.27 No.1

과채류 잔류성 농약의 돌연변이 유발성

고용구,김성홍,오창경,오명철,김수현 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-

Twelve residual pesticides which applied on the fruits and vegetables cultivation were tested for mutagenic activity using Salmonella typhimurium TA strain. Mutagenic activities against Tedion, Monopho, Danoton, Ometon and Captan of twelve pesticides tested were strong, Thalonil, Prosing, EPN, Phentoate, Dicopol and Captan were medium, and Dicopol were no. Tedion, hlonopho and Danoton showed strong mutagenic activities on TA98, Thalonil, Prosing, EPN, Phentoate, Parathion and Sappiran showed medium, and Danoton showed weak, while Dicopol and Sappiran showed no. At the other hand, Tedion, Danoton, Ometon and Captan showed storng mutagenic acitivities on TA100, Thalonil and Monopho showed medium and Prosing, Phentoate, Parathion and Sappiran showed weak, while EPN and Dicopol showed no. Therefore, EPN identified mutagen which induced only frameshift mutation, Sappiran was only base-substitution, and nine except these and Dicopol of twelve pesticides tested were mutagens which induced both frameshift and base-substitution mutation.

고등어부시의 제조에 관한 연구 : 1. 제조중의 정미성분의 변화

고신효,임희유,고용구,하진환 濟州大學校 工科大學 産業技術硏究所 1993 産業技術硏究報告 Vol.4 No.-

Mackerel-bushi was manufactured as a natural flavoring substance and in each processing step the taste compounds including nucleotides and their related copmounds. free amino acids, non-volatile organic acids, trimethylamine oxide, trimethylamhe and betaine were determined. IMP and inosine were abundant in Mackerel-bushi which marked 465.3㎎/l00g and 465.7㎎/l00g on dry basis while ADP was trace in content. 17 species of free amino acids were isolated and identified from the extracts of raw material and its products. The abun-dant amino acids in the extracts were lysine, taurine, phenylalanine and alanine, and those were consisted of 65.8~80.7% of the total free amino acids while isoleucine, aspartic acid, tyrosine and leucine were poor, and glycine was trace in content. Citric and lactic acids were 95% of total nonvolatile organic acids, and malonic, oxalic and α-ketoglutaric acids were poor. The content of TMAO and TMA in the extract of Mackerel-bushi were 11.9㎎/l0g and 13.5㎎/100g on dry basis and that of betaine was 93.1㎎/100g. The major minerals were Ca. Na. K. and Mg.

제주도산 山草, 海藻 및 버섯類 水溶性 抽出物의 抗癌效果

김수현,오창경,고용구,김성홍,오명철 제주대학교 1994 논문집 Vol.39 No.-

제주지역에서 생산되는 식물 중에서 파리풀(지방명;가신새), 삼지구엽초, 미역, 톳, 표고버섯 및 만년버섯(일명;영지)에서 열수 및 냉수추출물을 얻어 마우스 암모델 세포인 sarcoma-180 세포를 ICR 마우스에 이식하여 고형암 성장저지율과 수명연장율에 대한 항암성을 검색한 결과는 다음과 같다. 산초추출물의 항암효과는 파리풀에서 53.4%, 삼지구엽초에서 58.2%의 고형암 성장저지율을 보였다. 해조류추출물의 항암효과는 톳에서 63.1%, 미역에서 38.2%의 고형암 성장저지율을 보였다. 버섯추출물의 항암효과는 만년버섯에서 62.8%, 표고버섯에서 66.4%의 고형암 성장저지율을 보였다. 산초, 해조, 버섯류 각 추출물에서 복수형암에 대한 효과는 매우 낮았다. 모든 시료는 일정량을 초과 투여한 실험군에서 고형암 성장저지효과가 낮거나 수명이 단축되었다. The hot and cole water extracts were obtained from herbs (Phryma leptostachya and Epimedium koeranum), seaweeds(Hizikia fusiforme and Undaria peterseniana), and mushrooms(Gonoderma luciolum and Lentimus deodes) among which have long been known to have antitumor effects. These extracts were tested on growth inhibition rate(I.R) of solid tumor and prolongation rate(P.R) in animal experiment using ICR mouse with a mouse tumor models sarcoma-180 cell. The results were as follows ; The antitumor effects of herb extracts were 53.4% with Phryma leptostachya and 58.2% with Epimedium koeranum in growth inhibition rate of solid tumor. The antitumor effects of seaweed extracts were 63.1% with Hizikia fusiforme and 38.2% with Undaria peterseniana in growth inhibition rate of solid tumor. The antitumor effects of mushroom extracts were 62.8% with Ganoderma luciolum and 66.4% with Lentinus edodes in growth inhibition rate of solid tumor. The effects on ascite tumor of extracts from herbs, seaseeds and mushrooms were so low that no anititumor effect could be recognized. All the samples showed low growth inhibition rate of solid tumor or short life in over dose groups.

果采類 殘留性 農藥의 突然變異 誘發性

오명철,오창경,김성홍,고용구,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

Salmonella typhimurium TA 균주를 이용하여 과채류 재배에 많이 사용되는 12종의 잔류성 농약에 대한 돌연변이 유발성을 검정하였다. 본 연구에 사용된 12종의 농약 중 Tedion, Monopho, Danoton, Ometon 및 Captan에 대한 돌연변이 유발성은 강하였고, Thalonil, Prosing, EPN, Phentoate, Dicopol 및 Captan에 대한 돌연변이 유발성은 중간 정도였으나, Dicopol은 돌연변이 유발성이 없었다. TA98에서, Monopho와 Danoton은 강한 돌연변이 유발성을 나타내었고, Thalonil, Prosing, EPN, Phentoate, Parathion 및 Sappiran은 중간정도의 돌연변이 유발성, Danoton은 약한 돌연변이 유발성을 나타낸 반면, Dicopol과 Sappiran은 돌연변이 유발성을 나타내지 않았다. 한편, TA100에서는 Tedion, Danoton, Ometon 및 Captan이 강한 돌연변이 유발성을, Thalonil과 Monopho는 중간 정도의 돌연변이 유발성, Prosing, Phentoate, Parathion 및 Sappiran은 약한 돌연변이 유발성을 보인 반면, EPN과 Dicopol은 돌연변이 유발성이 없었다. 따라서, EPN은 구조이동성 돌연변이를, Sappiran은 염기치환성 돌연변이를, 그리고 이들과 Dicopol을 제외한 나머지 9종의 농약은 구조이동성 및 염기치환성 돌연변이를 일으키는 돌연변이원임을 확인하였다. Twelve residual pesticides which applied on the fruits and vegetables cultivation were tested for mutagenic activity using Salmonella typhimurium TA strain. Mutagenic activities against Tedion, Monopho, Danoton, Ometon and Captan of twelve pesticides tested were strong, Thalonil, Prosing, EPN, Phentoate, Dicopol and Captan were medium, and Dicopol were no. Tedion, Monopho and Danoton showed strong mutagenic activities on TA98, Thalonil, Prosing, EPN, Phentoate, Parathion and Sappiran showed medium, and Danoton showed weak, while Dicopol and Sappiran showed no. At the other hand, Tedion, Danoton, Ometon and Captan showed strong mutagenic activities on TA100, Thalonil and Monopho showed medium and Prosing, Phentoate, Parathion and Sappiran showed weak, while EPN and Dicopol showed no. Therefore, EPN identified mutagen which induced only frameshift mutation, Sappiran was only base-substitution, and nine except these and Dicopol of twelve pesticides tested were mutagens which induced both frameshift and base-substitution mutation.

고등어부시의 製造에 關한 硏究 : 1.製造중의 呈味成分의 變化

河璡桓,高容九,高臣孝,任禧雄 濟州大學校工科大學附屬産業技術硏究所 1993 尖端技術硏究所論文集 Vol.4 No.-

생합성 조미료의 안전성 문제 및 미각의 고급화, 다양화 추세에 따른 천연소재를 원료로 하는 풍미계 조미료의 수요가 늘고 있다. 본 연구에서는 고등어를 원로로하여 재래식 부시와 같은 형상을 중요시 하지 않고 우리나라 사람의 기호에 맞고 즉석 수우프의 원료로 이용할 수 있는 고등어 부시를 제조하여 제조공정중의 정미성분의 변화에 대하여 실험하여 다음과 같은 결과를 얻었다. 1. 고등어부시 제품의 수분함량은 8.1%, 조단백질, 조지방, 조회분은 각각 77.9%, 7.8%, 5.4%였으며, pH는 5.67 산도는 22.2㎖이었다. 2. 고등어부시 제품의 핵산관련물질 함량은 IMP가 건물량 기준으로 465.3㎎/100g, inosine 이 465.7㎎/100g이었으나 ADP는 흔적량에 불과하였다. 유리아미노산은 17종이 분리, 동정 되었는데 함량이 많은 것은 lysine, taurine 그리고 phenylalanine, alanine으로 이 등 4종의 아미노산이 총 유리아미노산의 65.8-80.7%를 차지하였으며 isoleucine, aspartic acid, tyrosine 및 leucine은 그 함량이 적었고 glycine은 흔적량이었다. 3. 불휘발성유기산 중에는 lactic acid, citric acid의 함량이 높아 이 2종의 유기산이 총 불휘발성유기산 함량의 95% 정도를 차지하였으며 malonic acid, oxalic acid, α-ketoglutaric acid는 미량 함유되어 있었다. TMAO, TMA는 건물량 기준으로 11.9㎎/100g및 13.5㎎/100g으로 미량 존재하였으며 betaine의 함량은 93.1㎎/100g이었다. 무기질 중에서는 Ca, Na, K, Mg의 함량이 비교적 많았다. Mackerel-bushi was manufactured as a natural flavoring substance and in each processing step the taste compounds including nucleotides and their related copmounds, free amino acids, non-volatile organic acids, trimethylamine oxide, trimethylamine and betaine were determined. IMP and inosine were abundant in Mackerel-bushi which marked 465.3㎎/100g and 465.7㎎/100g on dry basis while ADP was trace in content. 17 species of free amino acids were isolated and identified from the extracts of raw material and its products. The abun-dant amino acids in the extracts were lysine, taurine, phenylalanine and alanine, and those were consisted of 65.8~80.7% of the total free amino acids while isoleucine, aspartic acid, tyrosine and leucine were poor, and glycine was trace in conteent. Citric and lactic acids were 95% of total nonvolatile organic acids, and malonic, oxalic and α-ketoglutaric acids were poor. The content of TMAO and TMA in the extract of Mackerel-bushi were 11.9㎎/100g and 13.5㎎/100g on dry basis and that of betaine was 93.1㎎/100g. The major minerals were Ca, Na, K, and Mg.