혈중 납과 혈압의 연관성에 관한 메타분석

고상백,남정모,최홍열,차봉석,박종구,지호성,김춘배,Koh, Sang-Baek,Nam, Chung-Mo,Choi, Hong-Ryul,Cha, Bong-Suk,Park, Jong-Ku,Jee, Ho-Sung,Kim, Chun-Bae 대한예방의학회 2001 예방의학회지 Vol.34 No.3

Objectives : To integrate the results of studies which assess an association between blood lead and blood pressure. Methods : We surveyed the existing literature using a MEDLINE search with blood lead and blood pressure as key words, including reports published from January 1980 to December 2000. The criteria for quality evaluation were as follows: 1) the study subjects must have been workers exposed to lead, and 2) both blood pressure and blood lead must have been measured and presented with sufficient details so as to estimate or calculate the size of the association as a continuous variable. Among the 129 articles retrieved, 13 studies were selected for quantitative meta-analysis. Before the integration of each regression coefficient for the association between blood pressure and blood lead, a homogeneity test was conducted. Results : As the homogeneity of studies was rejected in a fixed effect model, we used the results in a random effect model. Our quantitative meta-analysis yielded weighted regression coefficients of blood lead associated with systolic blood pressure and diastolic blood pressure results of 0.0047 (95% confidence interval [CI]: -0.0061, 0.0155) and 0.0004 (95% CI: -0.0031, 0.0039), respectively. Conclusions : The published evidence suggested that there may be a weak positive association between blood lead and blood pressure, but the association is not significant.

사회적 지지가 만성적 스트레스와 면역체계에 미치는 영향

고상백,박종구,차봉석,장세진,Koh, Sang-Baek,Park, Jong-Ku,Cha, Bong-Suk,Chang, Sei-Jin 대한예방의학회 2002 예방의학회지 Vol.35 No.4

Objectives : To examine whether cumulative chronic stress influences the immune status, and to verify the effect of social support on the relationship between these two dimensions in male manufacturing workers. Methods : A total of 39 workers were recruited for this study. A structured-questionnaire was used to assess general characteristics, job characteristics (work demand and decision latitude), psychosocial distress, and social support. The serum levels of CD4 and CD8 were measured as immune markers, and were collected between 8:00 and 10:00am in order to standardize the markers. Nonparametric statistics were used to estimate the differences between job characteristics and the immune markers. Results : General characteristics, and health-related behaviors, were not associated with CD4, CD8 or CD4/CD8. No relationships were found between job characteristics and the mean levels of immune reactivity. These results were consistent, even after controlling for social support. Social support failed to modify the relationship toward work demand, decision latitude or psychosocial distress to CD4, CD8, and CD4/CD8. Conclusion : Cumulative chronic life stress might not influence the immune status, and the effects of social support on the immune function under chronic stress, may not play a crucial role in modifying the relationships. This implication supports that the effect of stress on the immune function may be determined by the characteristics of that stress. further research should effectively considers the type, magnitude and timing of a stress event, and modifiable factors, such as personality traits, coping style, and hormone excretion levels, on the alteration of immune status.

흰쥐에서 N,N-dimethylformamide에 의한 간장의 Microsomal Cytochrome P450의 유도

고상백,차봉석,강성규,정효석,김기웅,Koh, Sang-Baek,Cha, Bong-Suk,Kang, Seung-Kyu,Joung, Hyo-Seok,Kim, Ki-Woong 대한예방의학회 1999 Journal of Preventive Medicine and Public Health Vol.32 No.1

이 연구는 DMF에 의한 간독성 기전을 알아보기 위하여, 대사과정에서 중요한 역할을 하는 이물질 대사효소와 그와 관련된 효소가 어떠한 변화를 보이는가를 알아보았다. 이 연구에 사용된 동물은 Sprague Dawley계 수컷 흰쥐로 DMF를 체중 kg당 0(Control), 450 (D1), 900 (D2), 1,800 (D3) mg을 1일 l회씩 3일간 연속하여 복강주사하였다. 마지막 투여 후 24시간 후에 실험동물로부터 간장의 microsome을 분리하였고, P450 동위효소의 유도와 P450의존성 촉매 효소의 활성도 변화를 관찰하였다. 연구결과, DMF를 투여한 실험군이 대조군보다 microsomal 단백질 함량이 통계학적으로 유의하지는 않았지만 낮은 수치를 보였다. P450과 b5 함량 역시 대조군과 투여군간에 유의한 차이가 없었다. 대사과정에서 어떠한 전자전달계가 주로 관여하는지를 알아보았는데, NADPH-P450 reductase의 경우 대조군보다 투여군이 투여용량이 증가함에 따라 활성도가 유의하게 증가하였다(p<0.01). NADH-b5 reductase의 활성도 의 경우는 대조군보다 투여군이 감소하여(p<0.01), 전자전달이 주로 NADPHP-450 reductase에 의해 이루어지는 것을 알 수 있었다. 활성도 측정에서는 EROD 와 PROD 활성도는 유의한 차이를 보이지 않았으나 pNPH 활성도는 처리군에서 현저한 증가가 관찰되었다(p<0.01). 또한 P4501A1/2, P4502B1/2 및 P4502E1에 대한 단세포군 항제를 이용한 Western immunoblot 분석에서 P4502E1 단백질 의 양이 현저하게 증가하였다. 이 상의 결과를 보면, DMF에 의해서 P4502E1 형태의 동위효소가 유도되며, 유도된 P4502E1 동위효소가 DMF의 대사에 관여하는 것으로 보인다. Objectives. In order to gain a better understanding of the mechanism of DMF toxicity, recent studies have focused on hepatic drug metabolizing enzymes. In this study, we investigated the effects of DMF on the induction of P450 and the activities of other related enzymes in rat liver microsomes. Methods. DMF was administered to male Sprague Daweley rats by intraperitoneal injection at 0(control), 450(D1), 900(D2), 1,800(D3) mg DMF/kg body weight in olive oil once a day for three days. Hepatic P450 was measured by method of Omura and Sato. We evaluated selective assays for the three drug metabolizing cytochrome P450 isoenzymes 1A1, 2B1 and 2E1. Results. The content of microsomal protein, P450 and b5 were tended to be decreased in DMF treated group, but they were not statistically significant. The activity of NADPH-cytochrome P450 reductase was significantly increased dose dependently(p<0.01), but the activity of NADH-b5 reductase was decreased in the treated group(p<0.01). The activities of PROD and EROD were not significant between control and treated group. The activities of pNPH in the DMF treated groups were higher than that of the control group(p<0.01). When Western immunoblottings were carried out utilizing three monoclonal antibodies which were specific against P4501A1/1, P4502B1/2 and P4502E1, the strong density band corresponding to P4502E1 was observed with the microsomes obtained from the rats treated with DMF. But there were no significant increased in the P4501A1/2 and P4502B1/2 band densities in immunoblotting. Conclusions. These result suggested that P4502E1 was inducible by DMF and P4502E1 isozyme might be responsible for the hydroxylation of DMF to HMMF.

직장인들의 건강수준 평가를 위한 측정도구의 신뢰도와 타당도 분석

고상백,장세진,강명근,차봉석,박종구,Koh, Sang-Baek,Chang, Sei-Jin,Kang, Myung-Guen,Cha, Bong-Suk,Park, Jong-Ku 대한예방의학회 1997 예방의학회지 Vol.30 No.2

In order to test scaling assumption, and to assess the validity, reliability, and acceptability of the Short form 36(SF-36) health survey questionnaire, we conducted a survey. Samples were 296 workers who had been employed in small sized companies. All scale passed for item internal consistency(100% sucess rate) and item discriminant validity(100% success .ate). Reliability coefficients were ranged from the lowest 0.51 to the highest of 0.85. For 87.5% of the total workers, inconsistent responses were not observed. Only 3.0% of the total workers failed two or more checks. Factor analysis was performed using principal axis factor method and quartimax rotation. In this survey, the SF-36 retained available psychometric properties even when used in a generally healthy worker group. But further study with some consideration to develope health status measurement is expected : first, the definition of health status should be rationalized. Second, the measurement of outcome is an important consideration in evaluations of quality of care. But ambiguities hinder understanding of this important topic. Third, internal consistency should be interpreted with caution as an indication reliability because it ignores potentially important sources of variation that can occur over time.

직무스트레스의 조직적 관리

고상백(Sang Baek Koh),장세진(Sei Jin Chang),강동묵(Dong Mug Kang) 대한스트레스학회 2005 스트레스硏究 Vol.13 No.3

방광암과 Glutathione S transferase mu (GSTN1) 유전적 다형성에 관한 메타분석

고상백(Sang Baek Koh),차봉석(Bong Suk Cha),박종구(Jong Ku Park),김춘배(Chun Bae Kim),강명근(Myung Gun Kang),김기웅(Ki Woong Kim),이원진(Won Jin Lee),장성훈(Soung Hoon Chang) 대한직업환경의학회 1999 대한직업환경의학회지 Vol.11 No.1

직무스트레스와 심혈관계질환 위험요인과의 상관성

고상백(Sang Baek Koh),장세진(Sei Jin Chang),박종구(Jong Ku Park),박준호(Jun Ho Park),손동국(Dong Koog Son),현숙정(Sook Jung Hyun),차봉석(Bong Suk Cha) 대한직업환경의학회 2005 대한직업환경의학회지 Vol.17 No.3

사상체질에 따른 대사증후군과 Adiponectin의 상관성

유준상,고상백,박종구,Yu, Jun-Sang,Koh, Sang-Baek,Park, Jong-Ku 사상체질의학회 2009 사상체질의학회지 Vol.21 No.3

1. Objectives: The objective of this study is to investigate the relationship between adiponectin and metabolic syndrome according to Sasang Constitution. 2. Methods: Six hundred sixty six participants were included in this cohort study. Sex, age, BMI(Body Mass Index), smoking, drinking, adiponectin level and Metabolic syndrome components were considerd. Sasang constitutional diagnosis was carried out by a sasang constitutional specialist using photos, questionnaires and PSSC(Phonetic System for Sasang Classification). 3. Results: In binary logistic analysis after adjustment of age, sex, BMI, smoking, drinking, adiponectin level and sasang constitution were related with Metabolic syndrome. 4. Conclusions: We suggest that adiponectin and sasang constitution are the important biomarker in Metabolic syndorme.

40세 이후의 사망에 영향을 주는 요인에 관한 코호트내 환자-대조군 연구

박종구,고상백,김춘배,박기호,왕승준,장세진,신순애,강명근,Park, Jong-Ku,Koh, Sang-Baek,Kim, Chun-Bae,Park, Kee-Ho,Wang, Seung-Jun,Chang, Sei-Jin,Sin, Soon-Ae,Kang, Myung-Guen 대한예방의학회 1999 Journal of Preventive Medicine and Public Health Vol.32 No.3

Objectives: This study was conducted to identify the factors influencing the mortality of Koreans over the age of 40 by a nested case-control study. Methods: The cohort consisted of the beneficiaries of Korea Medical Insurance Corporation for Government Employees & Private School Teachers and Staff(KMIC) who received health examinations of KMIC in 1992 and 1993 retrospectively. At that time, they were more than 40 years old. The cases were 19,258 cohort members who had died until December 31, 1997. The controls were 19,258 cohort members who were alive until December 31, 1997. Controls were matched with age and sex distribution of the cases. The data used in this study were the funeral expenses requesting files, and the files of health examinations and health questionnaires gathered in 1992 and 1993. To assess the putative risk factors of death, student t-test, chi-square test, multiple logistic regression analysis were used. Results : In multiple logistic regression analysis, independent risk factors of death were as follows; systolic blood pressure, diastolic blood pressure, blood glucose, AST, urine glucose, urine protein, alcohol drinking(frequency), cigarette smoking and perceived health status, intake of restoratives and blood transfusion showed positive associations with death; coffee consumption showed negative associations with death; and body mass index and serum total cholesterol showed J-shaped association with death. Conclusions: Regarding the direction of association, the result of analysis on the data restricted to '96-'97 was same as that of '93-'97. But in some variables such as obesity, serum cholesterol, the odds ratios of death in the data of '96-'97 were higer than those of '93-'94, which suggested that the data of '93-'94 was bearing effect-cause relationship. We concluded that it suggested further researches using long-term follow-up data to be needed in this area. 공 교의료보험 적용대상자들 중 1992년 건강검진 수검 피보험자와 1993년 건강검진 수검 피부양자를 대상으로 사망에 영향을 미치는 요인에 관한 코호트내 환자-대조군 연구를 실시한 결과는 다음과 같다. '93-'97년도 자료의 분석에서 사망과 정상관 관계를 보인 변수는 수축기 혈압, 이완기 혈압, 혈당, AST, 요당, 요단백, 음주('자주 마신다'), 흡연, 건강인지, 영양제 복용, 수혈이었다. 사망과 역상관 관계를 보인 변수는 커피 음용이었다. 비만도와 혈중 콜레스테롤은 사망과 J형의 상관관계를 보였다. '96-97년 자료만을 이용하여 분석한 경우도 '93-'97년 자료와 관련성의 방향은 동일하였으나, 비만$(25-30kg/m^2)$과 콜레스테롤(240 mg/dL이상)의 사망 예방효과는 '93-'97년 자료에 비해 '96-'97년 자료에서 감소함으로써 '93-'94년 자료에 결과-원인 관계가 일부 개재되었을 가능성을 배제할 수 없었다.

크실렌 노출로 인한 요중 메틸마뇨산 배설에 미치는 유전자 다형성 연구

김청식,고상백,김형수,박수경,장성훈,Kim, Cheong-Sik,Koh, Sang-Baek,Kim, Hyeong-Su,Park, Sue-Kyung,Chang, Soung-Hoon 대한예방의학회 2004 예방의학회지 Vol.37 No.4

Objectives : The purpose of this study was to investigate the effect of genetic polymorphism of cytochrome P450 2E1 (CYP2E1) and aldehyde dehydrogenase 2 (ALDH2) on the xylene metabolism. Methods : Among 247 workers, 116 were occupationally exposed to xylene and 131 were not. Workers exposed to xylene had different work such as spray, touch-up, mix & assist, and pre-treat. Questionnaire variables were age, sex, use of personal protective equipment, smoking, previous night's drinking and work duration. The urinary methylhippuric acid was measured in the urine collected in the afternoon and corrected by urinary creatinine concentration. The genotypes of CYP2E1 and ALDH2 were investigated by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) methods with DNA extracted from venous blood. Results : 1. The urinary concentrations of o-, m-, and p-methylhippuric acid and total methylhippuric acid in the exposed group were significantly higher than those in the non-exposed group (p<0.001). 2. In multiple regression analysis, the urinary methylhippuric acid concentration was significantly influenced by exposure grade (Job-exposure matrixes), smoking, drug use and kind of protective equipment (p<0.1). 3. Genetic polymorphism of CYP2E1 and ALDH2 did not affect urinary methylhippuric acid level in the exposed group (p>0.05). Conclusions : Exposure grade, smoking, drug use and kind of protective equipment affected urinary methylhippuric acid level, whereas genetic polymorphism of CYP2E1 and ALDH2 did not. However, further investigation for the effect of genetic polymorphism on the metabolism of xylene with a larger sample size is needed.