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제주지역의 akabane virus에 대한 역학조사 및 원인체 분리동정
강완철 ( Wan Cheul Kang ),김은주 ( Eun Joo Kim ),현관종 ( Kwan Jong Hyun ),전창익 ( Chang Ik Cheon ),김희석 ( Hye Seck Kim ),이두식 ( Du Sik Lee ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.1
In this experiment, we studied the sero-positive rate of akabane virus in cattle from Jeju-do and analyzed the seroepidemiological features. In an analysis of 1051 samples, the positive rate for neutralizing antibody in sera collected in nine regions on Jeju-do was 56.7%. The rate varied with the region. The positive rate was 69.6% in Aewol, 63.1% in Jeju city, 54.4% in Anduck, 51.0% in Hallim, 69.8% in Jocheun, 47.6% in Pyosun, 40% in Daejeong, 30.0% in Flankyung, 71.6% in Namwon, 24.5% in Sungsan, 83.3% in Seokypo and 44.5% in Gujwa, respectively. The rate also depended on the age of the cattle. The positive rate was 67.2% in calves 0- to 12-month old, 48.3% in cattle 13- to 24-month old, 65.4% in cattle 25- to 36-month old, arid 65.4% in cattle more than 37 months old. To isolate the virus from calves with malformations including arthrogryposis and hydranencephaly, cerebral homogenates were inoculated into Vero cells, which were determined for cytopathic effect (CPE). Vero cells with CPE were examined for Akabane virus using an electron microscope (EM) and indirect immunofluorescent antibody test (WA). Typical virus particles with a width of 90-l3Onm and specific immunofluorescence in the cytoplasm of infected cells were sought for identification.
제주도내 자연발생한 말유산태아에서 PCR을 이용한 Equine Herpesvirus Tyre-1 검출
문혁,강완철,김은주,김진희,고현주,양재혁,손원근,이두식,Moon, Hyuk,Kang, Wan-Cheul,Kim, Eun-Joo,Kim, Jin-Hoe,Ko, Hyun-Joo,Yang, Jae-Hyuk,Son, Won-Geun,Lee, Du-Sik 한국동물위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine Industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.
양형석,양나연,강완철,강상철,강홍원,김재훈,배종희,Yang, Hyoung-Seok,Yang, Na-Yeon,Kang, Wan-Cheul,Kang, Sang-Chul,Kang, Hong-Won,Kim, Jae-Hoon,Bae, Jong-Hee 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.2
Total 160 head of porcine kidneys were examined for gross and histopathological lesions and polymerase chain reaction (PCR) for porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV), Leptospira species and porcine reproductive and respiratory syndrome virus (PRRSV). Grossly, 137 kidneys (85.6%) had lesions characterized by the presence of the scattered white foci. Microscopically, multifocal interstitial nephritis, which classified into 4 grades such as, no lesion (Score 0), mild lesion (Score 1), moderate lesion (Score 2) and severe chronic lesion (Score 3) with fibrosis, was observed in 159 cases (99.4%). The histopathologic mean score for multifocal interstitial nephritis was significantly different (P<0.05) between the cases of PCV-2 single infection and the cases of co-infection with PCV-2 and PPV. According to PCR evaluation, PCV-2 were detected in 73.8% (118 cases), PPV were in 66.9% (107 cases), however Leptospira spp. and PRRSV were negative in all kidneys. Both PCV-2 and PPV were detected in 52.5% (84 cases). In 84 cases co-infected with PCV-2 and PPV, the occurrence of lymphoid follicle and vasculitis were observed as 65.5% (55 cases) and 26.2% (22 cases), respectively. These results revealed that PCV-2 and PPV were major infectious agents for interstitial nephritis in slaughtered pigs, Jeju. And the histopathologic lesions of multifocal interstitial nephritis were more severe in the case co-infected with PCV-2 and PPV.
제주도내 자연발생한 말유산태아에서 PCR을 이용한 Equine Herpesvirus Type-1 검출
문혁 ( Hyuk Moon ),강완철 ( Wan Cheul Kang ),김은주 ( Eun Joo Kim ),김진회 ( Jin Hoe Kim ),고현주 ( Hyun Joo Ko ),양재혁 ( Jae Hyuk Yang ),손원근 ( Won Geun Son ),이두식 ( Du Sik Lee ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.
제주지역 양돈장에서 발생한 자돈 설사병 바이러스의 면역조직화학적 확인
안미정 ( Mee Jung Ahn ),김진희 ( Jin Hoe Kim ),강완철 ( Wan Cheul Kang ),강종철 ( Jong Cheul Kang ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3
In an outbreak of acute porcine diarrhea in newborn piglets, an etiological study was carried out using piglets submitted in Cheju Province Institute for Livestock Promotion(Cheju Veterinary Service for the disease diagnosis). Sixteen piglets(2-7 days old) were collected from 4 farms during outbreaks of diarrhea disease(from January to April 2000). Specimens were taken after necropsy and examined by inimunohistochemistry using of monoclonal antibodies for porcine epidemic diarrhea(PED) virus, transmissible gastroenteritis(TGE) virus, and porcine rotavirus. Immunohistochernistry showed that PED virus antigens, but both TGE virus and rota virus antigens not, were localized in the some epithelial cells of the intestines of 14 animals among 16 piglets examined. PED virus antigens were mainly detected in the cytoplasm of enterocytes. Infected cells, which were most abundant in the villous epithelial cells of the jejunum and ileum, were uncommon in the crypt, epithelial cells, the lamina propria and Peyer` s patches of piglets examined. The results suggest that PED virus is one of the most prevailing agents in an outbreak of fatal diarrhea in newborn piglets on Cheju island and PED virus was need to further study to prevent this disease.