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내마모 구조 코팅용 Cr-Al-Si-N 코팅막의 미세구조와 기계적 특성에 관한 연구
강동식,김광호,Kang, Dong-Shik,Kim, Kwang-Ho 한국재료학회 2005 한국재료학회지 Vol.15 No.11
Cr-Al-Si-N coatings were deposited on WC-Co substrates by a hybrid coating system of arc ion plating and DC magnet :on sputtering technique in $N_2/Ar$ mixture. The Cr-tll-Si-N coatings were synthesized with different Si contents. Their microstructure and mechanical properties were systematically investigated. The average size of crystallites largely decreases with the increase of Si content compared with Cr-Al-N. The microhardness of Cr-Al-Si-N coatings largely increases from 24 to 55 GPa. The enhanced hardness is believed to originate from the microstructural change by the fine composite microstructure of Cr-Al-N coatings with Si addition. The average friction coefficient of Cr-Al-Si-N coatings decreases from 0.84 to 0.45 with increasing Si content up to $16\;at.\%$.
Postnatal Developmental Expression of TEM7 mRNA and its Putative Ligand in the Rat Cerebellum
강동식(Dong Sik Kang),서인애(In Ae Seo),이현경(Hyun Kyoung Lee),정진용(Jin Yong Chung),김종민(Jong-Min Kim),이규열(Kyu Yeol Lee),박환태(Hwan Tae Park) 대한해부학회 2005 Anatomy & Cell Biology Vol.38 No.5
Tumor endothelial marker 7 (TEM7)는 암세포의 혈관내피세포와 소뇌 신경세포에서 특이적으로 발현되는 세포막 단백질이다. 본 연구에서는 in situ hybridization과 ligand binding assay를 이용하여 발생중인 흰쥐소뇌에서 TEM7 mRNA와 그 가상적인 리간드의 발현을 조사하였다. 분비되는 형태로 만들어진 TEM7 세포외부분의 재조합 단백질을 이용하여 리간드의 분포를 조사하였다. TEM7의 가상적인 리간드는 소뇌 조롱박세포의 가지돌기가 존재하는 분자층에 특이적으로 분포하였다. 발생학적인 연구는 조롱박세포에서 TEM7 mRNA의 발현이 나이에 따라 증가함을 보여 주었고, 분자층에 존재하는 가상적인 리간드는 출생후 발육과정 전 기간에 걸쳐 존재하였다. 본 연구결과는 TEM7과 리간드의 상호작용이 출생 후 발달과정동안 조롱박세포의 분화에 중요한 기능을 함을 보여준다. Tumor endothelial marker 7 (TEM7) is a putative transmembrane protein that is highly expressed in the tumor endothelium and cerebellar neurons. In the present study, the expression profile of TEM7 mRNA and its putative ligand in the developing cerebellum of the rat was investigated using in situ hybridization and ligand binding assay. The secreted recombinant ectodomain of TEM7 was employed to label the expression of putative ligand of TEM7 in the cerebellum. The expression of a putative ligand of TEM7 demonstrated by using TEM7 ectodomain was found in the molecular layer of the cerebellum, where the dendritic trees of Purkinje cells are present. A developmental study has shown that TEM7 mRNA expression in the Purkinje neurons was increased with age during postnatal development, whereas the putative ligand labeling in the molecular layer was observed throughout the developmental period. These findings indicate that TEM7-ligand interaction plays a role in the differentiation of Purkinje cells during postnatal development.
흰쥐 앞뇌에서 Tumor endothelial marker 7의 분포에 관한 면역조직화학 연구
강동식(Dong Sik Kang),이현경(Hyun Kyeng Lee),박혜경(Hye Kyung Park),서인애(In Ae Seo),이규열(Kyu Yeol Lee),박환태(Hwan Tae Park) 대한해부학회 2004 Anatomy & Cell Biology Vol.37 No.5
Tumor endothelial marker 7 (TEM7)은 종양의 혈관내피세포에서 특이적으로 발현되는 단백질군 중의 하나이다. 본 연구에서는 TEM7의 신경계에서의 기능과 발현을 이해하기 위해서 TEM7에 대한 polyclonal antibody를 제작하여 흰쥐 앞뇌(forebrain)에서 TEM7의 발현을 연구하였다. TEM7의 세포외 부분의 일부를 항원으로 이용하여 제작한 항체는 Western blot과 면역조직화학법에 활용될 수 있었다. 흰쥐 전뇌에서 TEM7은 대뇌피질, 해마, 시상하부 등의 신경세포에 분포하였다. 뇌의 투사신경세포에서 TEM7은 세포체와 가지돌기에 주로 존재하였다. 하지만 뇌아교세포, 혈관세포 그리고 뇌막 등에서는 관찰되지 않아 TEM7은 뇌에서 신경세포 특이적인 기능을 할 것으로 생각된다. Tumor endothelial marker 7 (TEM7) is a putative transmembrane protein that is highly expressed in the tumor endothelium. In the present study, the expression profile of TEM7 in the rat forebrain was investigated using immunohistochemistry with a specific polyclonal antibody against the extracellular region of TEM7. The immunohistochemical research revealed that TEM7 expressions were localized to specific neuronal areas such as cerebral cortex, hippocampus and hypothalamic magnocellular nuclei. The TEM7 protein was mainly present in the dendrite and cell body of the projection neurons. However, glial cells, vascular endothelial cells and meningeal cells did not show the expression of TEM7, indicating the specific roles of TEM7 in the neuronal cells in the vertebrate nervous system.
하이브리드 코팅시스템에 의해 제조된 Ti-Cr-Si-N 박막의 미세구조 및 기계적 특성연구
강동식(Dong Shik Kang),전진우(Jin Woo Jeon),송풍근(Pung Keun Song),김광호(Kwang Ho Kim) 한국표면공학회 2005 한국표면공학회지 Vol.38 No.3
Quaternary Ti-Cr-Si-N coatings were synthesized onto steel substrates (SKD11) using a hybrid method of arc ion plating (AIP) and sputtering techniques. For the Syntheses of Ti-Cr-Si-N coatings, the Ti-Cr-N coating process was performed substantially by a multi-cathodic AIP technique using Cr and Ti targets, and Si was added by sputtering Si target during Ti-Cr-N deposition. In this work, comparative studies on microstructure and evaluation of mechanical properties between Ti-Cr-N and Ti-Cr-Si-N coatings were conducted. As the Si was incorporated into Ti-Cr-N coatings, the Ti-Cr-Si-N coatings showed largely increased hardness value of approximately 42 ㎬ than one of 28 ㎬ for Ti-Cr-N coatings. The average friction coefficient of Ti-Cr-N coatings largely decreased from 0.7 to 0.35 with increasing Si content up to 20 at. %. In addition, wear behavior of Ti-Cr-N coatings against steel ball was much improved with Si addition due to the surface smoothening effect and tribo-chemical reaction.