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韓光燮,崔在乙 충남대학교 농업과학연구소 1989 농업과학연구 Vol.16 No.1
Twelve isolates of bacteria obtained from infected onions were classified into genus Erwinia based on diagnostic characteristics. Of twelve isolates studied, five were identified as E. carotovora subsp, carotovora, six as E. rhapontici and one as E. chrysanthemi on the bases of bacteriological properties. Symptoms caused by the genus Erwinia were different to be identified among the species. Therefore, we propose to name the disease of onion caused by E. carotovora subsp. carotovora, E. rhapontici and E. chrysanthemi as "bacterial soft rot of onion".
圃場과 收穫 後에 가지科 菜蔬에 무름病을 일으키는 病原細菌의 同定
崔在乙,韓光燮 충남대학교 농업과학연구소 1991 농업과학연구 Vol.18 No.2
The pathogenic bacteria isolated from the rotted fruits and tubers of red pepper, tomato, eggplant and potato which were collected from farms of Chungnam province and the markets of Seoul, Pusan, Taejon, Chonju, Iri, Weonju, Jecheon, Chungju and Cheonan in Korea. These isolates were divided into ninety-eight isolates of Erwinia sp. and seven isolates of Pseudomonas spp. on the basis of their taxonomic characteristics. Among seven isolates of Pseudomonas spp., six isolates were identified as P, viridiflava and two were P. marginalis and ninety-eight isolates of Erwinia sp. as E. carotovora. subsp. carotovora on the basis of bacteriological properties.
Plant Cell Wall as an Inducer of Pectate Lyase of Erwinia rhapontici
Choi, Jae Eul,Han, Kwang Sup 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
Pectate lyase(Pel)는 펙틴과 펙틴산을 분해하며, 기주식물의 감염에 관여한다. Erwinia rhapontici에 있어서 기주와 병원균의 병원성과의 상호관계를 구명하기 위하여 pectate lyase(Pel) 활성에 미치는 식물체 추출물과 세포벽의 효과를 검토하였다. 본 균은 glycerol이 포함된 minimal salts(MSG) 배지와 식물체 추출물이 첨가된 MSP 배지에서는 Pel 활성이 검출되지 않았다. 그러나 배추, 상추 잎, 감자 괴경, 셀러리 잎자루, 양파 인경, 당근 뿌리의 세포벽이 첨가된 MSP 배지에서는 Pel의 활성이 검출되었다. Pel을 유도하는 식물 인자는 불용성이고, 열처리에 불안전하였다. Pectate lyase (Pel) depolymerizes pectin and polygalacturonate and is thought to play a role in bacterial invasion into host plants. Effects of plant extracts and cell walls on the induction of Pel from Erwinia rhapontici strain 1 were wxamined to know hostparsite relationships associated with the pathogenicity of E. rhapontici. Pel activity was detected neither in minimal salts glycerol (MSG) medium nor in minimal salts polygalacturonate (MSP) medium containing plant extracts. Pel activity, however, was detected in MSP media amended with cell walls of Chinese cabbage, lettuce leaves, potato tubers, celery petioles, onion bulbs and carrot roots at the late stage of the bacterial culture. The Pel-inducing plant factors were water-insoluble and heat-labile.