연조직 증강을 위한 세포치료 시 비계로서 키토산의 온도 감응성 및 섬유모세포의 증식에 미치는 영향에 관한 연구

김정호,최진영,Kim, Jung-Ho,Choi, Jin-Young 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.3

Traditional surgical method or injection using filler is performed for soft tissue augmentation. Surgical methods have disadvantage of surgical morbidity. Commercially available injectable materials have the disadvantages such as resorption, short-term effect. repeated application and hypersensitivity. Significant shortcoming of cell therapy using autologous fibroblasts is delay of treatment effect. Chitosan/${\beta}$-glycerol phosphate (GP) solution has thermosensitive property and allows sol-gel transition at physiologic pH and temperature. These properties may resolve the delay of treatment effect. The purposes of this study are to evaluate the viscosity and pH changes of chitosan/${\beta}$-GP solutions and to evaluate the effect of chitosan/${\beta}$-GP solution on fibroblast proliferation and production of collagen. We measured the viscosity and pH as function of temperature, of the solution containing 1:0.7, 1:0.75, 1:0.8 chitosan (1, 10, 100, 700 kDa) /${\beta}$-GP. Fibroblasts from ears of 5 rats were cultured in chitosan/${\beta}$-GP solutions for 3 weeks. Cell proliferation and collagen contents were measured every week with WST (water-soluble tetrazolium salt) assay and Collagen assay respectively. The Results are 1) Chitosan(100 kDa<)/${\beta}$-GP solution (1:0.75) showed sol-gel transition at physiologic pH and body temperature and injectable properties. It will enable to resolve the delay in treatment effect 2) Cell proliferation and total collagen contents of the control group were increased with time. However, these decreased after the 1st week in experimental group 3) Collagen contents in the experimental group are higher than that of control group. Chitosan/${\beta}$-GP solution may provide favorable conditions for cell function

대규모 시스팀의 해석 및 제어에 관한 연구동향

김정호,정태진,정하재,Kim, Jeong-Ho,Jung, Tae-Jin,Jung, Ha-Jae 한국전자통신연구원 1988 전자통신동향분석 Vol.3 No.1

대규모 시스팀의 해석 및 제어에 관한 연구동향을 시스팀의 축소화 기법, 안정도 검사, 비집중 제어, 계층적 제어로 기술하고, 이러한 대규모 시스팀의 연구분야로 자동생산 시스팀, 전력계통시스팀, 교통통제 시스팀, 사회공학 시스팀 분야로 분류하고 그 해석 및 제어에 관한 연구동향을 분석함으로써 이와 관련하여 추진되고 있는 연구분야를 소개하였다.

난관 세포와 공동 배양에 의한 배 세포 발달의 향상

김정호,홍기연,김기석,최정훈,민부기,Kim, Jung-Ho,Hong, Gi-Youn,Kim, Kie-Sock,Choi, Jung-Hoon,Min, Bu-Kie 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

To improve in vitro embryonic cell development, this study was desigend to culture in vitro fertilized early embryos of mouse in two different systems; conditioned medium alone and ampullary cells co-culture. Thirty two of 83 embryos(38.6%) were blocked in the 2 cell stage by co-culture, as compared to forty of 42 embryos(95.2%) in control group for 24hours culture. And all the embryonic cells cultured for conditioned medium alone were blocked for 48 hours culture. Twenty seven of 46 embryos (58.7 %) which overcome culture block in 2 cell stage by cocultured were developed morular and expanded blastocyst, and ninteen of 46 embryos(26.1 %) underwent hatching for 96 hours culture. The cellular fragmented rates for embryo were 26.2% in medium alone; 10 fragmented blastomere were graded mild status and 1 fragmented blastomere in severe status. On the other hand, the fragmented rate for 48 hours co-cultured were 15.7%03/83); 8 fragmented embryos were graded mild status, moderate status in 3 fragmented embryos and severe in 2 fragmented embryos respectively. In conclusion, the co-culture of embryos with ampullary cells is good to improve quality of embryos and overcome of culture block as well as development of cell cleavage.

A Study of the Two-Dimensional High-Performance Liquid Chromatogrphy for Total Amino Acid Sequencing of rbST

김정호,김훈주,김준,Kim, Jung-Ho,Kim, Hoon-Joo,Kim, Joon 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

트립신으로 절단한 재조합 소성장호르몬의 펠타이드 조각을 분리, 정제하기 위하여 강이온 교환 HPLC와 역상 HPLC의 2차원 고성능 액체 크로마토그래피법을 이용하였다. 1차원적으로 역상 HPLC만을 이용해 펩타이드를 분리하였을 때에는 두 가지 이상의 서로 다른 펩타이드가 섞여 있는 heterogeneous peak이 발견되였다. 그러나 2차원 HPLC에 의해 펩타이드를 분리 시켰을 때에는 고도로 정제된 펩타이드를 얻을 수 있였다. 트립신 절단 결과 생긴 펩타이드를 모두 분리시키기 위해 1차적으로 강 양이온 교환 HPLC를 시행하였다. 이 결과 9개의 서로 다른 peak를 분리해 냈으며, 각각의 peak들을 다시 역상 HPLC하여 2차적인 분리를 시도하였다. 여기에서 얻은 peak들을 아미노산 서열분석을 통해 명료한 서열 분석결과를 얻어 낼 수 있었다. 또한 이 과정을 통해 53번 Cys과 164번 Cys 그리고 181번 Cys 사이에 disulfide bond가 존재하고 있음도 동시에 확인할 수 있었다. In order to separate and purify tryptic digested recombinant bST (rbST), 2-dimensional HPLC which involves a combination of strong cation-exchange chromatography and reverse-phase chromatography was applied. A single dimension RP-HPLC resulted in several heterogeneous peaks. However, all fractions purified by two-dimensional chromatogrphy resulted in homogeneous peptides and unambiguous sequence data. With a view of separating perfectly, tryptic digest was first fractionated by strong cation-exchange chromatogrphy. The nine fractions were obtained from this chromatogrphy and each of these fractions was applied to RP-HPLC. Fractions yielded from RP-HPLC were submitted for direct sequence analysis and clear sequence data were obtained. In this study, the location of disulfide bonds, Cys53-Cys164 and Cys181-Cys189, also were confirmed in rbST.

단열성능 향상 콘크리트 배합설계 및 생산기술 개발

김정호,박영신,Kim, Jung-Ho,Park, Young-Shin 한국콘크리트학회 2013 콘크리트학회지 Vol.25 No.6

진동/음향 해석에 의한 스피커의 음향특성 연구

김정호,김준태,김진오,민진기,Kim, Jung-Ho,Kim, Jun-Tai,Kim, Jin-Oh,Min, Jin-Ki 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.10

The acoustic characteristics of a direct radiator type loudspeaker has been studied in this paper. The natural modes of the speaker cone vibration analyzed numerically by the finite element method have been verified by comparing them with experimental results. The so-ap-proved finite-element model has been used to calculate the vibration response of the cone excited by the voice coil. The vibration displacement of the speaker cone paper has been converted into the vibration velocity and used as a boundary condition for the acoustic analysis. The frequency characteristics, directivity, and sound pressure distribution of the loudspeaker have been calculated by the boundary element method. The numerical results have been verified by the experiments carried out in an anechoic chamber. The variations of the acoustic characteristics due to the changes of some design parameter values can be examined using the numerical model.

한국경제(韓國經濟)의 구조변화(構造變化) 전망(展望): 다부문모형(多部門模型)의 모의실험(模擬實驗)

김정호,박준경,Kim, Jung-ho,Park, Jun-kyung 한국개발연구원 1992 韓國 開發 硏究 Vol.14 No.2

한국경제(韓國經濟)는 과거 30여년간 고성장(高成長)을 지속하여 왔는데, 이 고성장의 기반은 주로 제조업(製造業)의 양적(量的) 성장(成長)에 기인한 생산성(生産性) 향상(向上)이었다. 개발초기의 한국경제는, 노동(勞動) 원재료(原材料) 집약적(集約的)인 저가공(低加工) 저부가가치형(低附加價値型) 산업(産業)의 수출신장에 따른 기업규모의 확대에 의하여 규모(規模)의 경제성(經濟性)을 실현하는 과정에서 획득된 생산성(生産性) 향상(向上)에 기인하여 고성장(高成長)을 유지할 수 있었다. 1989년 이후의 성장세 둔화는 그동안 고성장의 기반이었던 노동(勞動) 원재료집약적(原材料集約的) 산업구조(産業構造)에 대한 구조적(構造的) 불균형(不均衡) 및 한계(限界)를 나타내기 시작하였으며, 이는 한국경제가 장기적이고 지속적인 안정적(安定的) 성장(成長)을 유지하기 위해서는 산업(産業)의 기술(技術) 지식집약화(知識集約化)가 가속되고 기술(技術) 지식집약적(知識集約的) 산업(産業)으로 자원이 재배분되는 산업조정과정(産業調整過程)이 원활히 진행되어야 하는 단계에 이르렀음을 시사한다. 개방화(開放化) 및 국제화(國際化)에 따른 내수시장(內需市場) 및 국제시장(國際市場)에서의 경쟁력을 유지하기 위해서도 국내산업구조(國內産業構造)는 개도국(開途國)의 수출상품(輸出商品)과 차별화(差別化)가 가능한 기술(技術) 지식집약적(知識集約的)인 고부가가치제품(高附加價値製品)의 개발(開發)을 필요로 한다. 따라서, 중(中) 장기(長期)의 산업구조(産業構造)에 대한 전망을 통한 경제동향의 분석(分析) 예측(豫測) 및 중(中) 장기(長期) 경제정책(經濟政策)의 수립(樹立)이 절실히 요구되고 있으며, 이 요구에 부응하기 위해 본고는 산업연관표(産業聯關表)를 중심으로 산업간 연관관계에 기초하여 산업정보자료(産業情報資料)를 이용한 다부문모형(多部門模型)을 사용하여 중(中) 장기(長期)의 구조변화(構造變化)를 분석(分析) 전망(展望)하였다. The Korean economy has sustained high growth rate for almost three decades, that have been associated with the rapid expansion of manufacturing industries. In the beginning stage of development, the high growth of the Korean economy has been based on improvements in productivity obtained by the economies of scale. In that period, the improvements in productivity could be secured by the economies of scale in the export-oriented industries which are the labor- and material-intensive industries. In the latter half of the 1980s, the Korean economy went through rapid transition. Now Korea is at another juncture in its development process, where economic restructuring is critical to sustain high growth. However, economic restructuring in the 1990s call for much more concerted effort than before, since changes in internal and external conditions have profoundly altered the environment for economic development. If Korea is to sustain high growth in the 1990s, it has to promote balanced economic and social development in coordination with the smooth facilitation of industrial restructuring. There are no inherent conflicts among the issues involved, so they can be resolved by restructuring the economy to facilitate, in a global context, the development of knowledge- and technology-intensive activities and to ensure that the benefts of growth are reflected of qualitative improvements in national living standards. In this paper, we examined a scenario of structural changes using a mid- and long-term multisector model, in order to understand the conditions needed for realizing the growth potential. This examination explains the important features of the development course and policy directions that will help sustain high growth in the 1990s.

Bar code 활용기술 분석

김정호,박중무,이대기,Kim, Jeong-Ho,Park, Jung-Mu,Lee, Dae-Gi 한국전자통신연구원 1989 전자통신동향분석 Vol.4 No.4

슈퍼나 소매점등에서 판매되는 상품의 포장을 자세히 살펴보면 흑색과 백색의 줄무늬가 인쇄되어 있는 것을 볼수가 있다. 이것을 bar code라고 하는 데 국명, 메이커명, 상품명등을 수치화하여 하나의 코드로 만들고 그것을 독해하여 가격을 산출하고 정산업무를 신속하고 정확하게 처리하면서 자료를 모아 해석하여 재고 관리, 발주, 매입 업무를 합리화하고 이를 정보 네트워크에 의하여 수요에 대응한 판매를 전개하는 등 물자의 유통관리에 폭넓게 이용되고 있다. 즉 bar code system은 흑색 또는 백색의 굵은 bar와 가는 bar의 구성에 의하여 하나의 성질 (숫자, 기호, 알파벳 문자)을 나타내고 그 연속을 광학식 입력수단을 통하여 하나의 코드에 정리한 자동화된 데이터수집(Automated Data Collection)방법으로 활용되고 있다. 본고에서는 이러한 bar code의 동작, 구성, 인식장치의 원리와 활용사례를 기술하였다.

외상 후 발생한 심한 족관절 첨족 변형의 2단계 수술적 치료방법 - 예비보고 -

김정호,이준영,하상호,유재원,이상홍,나웅채,Kim, Jung-Ho,Lee, Jun-Young,Ha, Sang-Ho,You, Jae-Won,Lee, Sang-Hong,Na, Woong-Chae 대한족부족관절학회 2011 대한족부족관절학회지 Vol.15 No.2

Purpose: To evaluate the effectiveness as well as correct the post-traumatic severe ankle equinus deformity by conducting the treatment surgery, which is divided into 2 stages, soft tissue adhesiolysis and ankle arthrodesis. Materials and Methods: We have conducted the methods, which are Z-plasty Achilles tendon lengthening, multiple capsulotomy and tendon lengthening (flexor hallucis longus muscle, flexor digitorum longus, posterior tibialis tendon) for 10 patients who has shown equinus deformity after post-traumatic compartment syndrome due to the injury. The average age of patients was 33.7 year-old; there were 8 men and 2 women, and the follow up period was 13 months (6~31 mon). Outcomes were rated based on American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, Visual Analogue Scale (VAS) and patient's satisfaction after operation. Results: The average degree of preoperative equinus deformity was 64 degree (-60~-70), and we've obtained AOFAS anklehindfoot score that was evaluated after 4 months of 2nd operation which was 76.7 score on average. We've also checked the decreased pain score from all of the patients according to the fact that the average VAS before the surgery was 6 (4~8), but it reduced to 3 (1~4). The patient's satisfaction showed generally great satisfactions which was 5 cases were excellent, 2 cases were good, and 3 cases were fair. Conclusion: The two staged surgical treatment of post -traumatic severe ankle equinus deformity, which was conducted of soft tissue adhesiolysis and arthrodesis, could be one of the effective methods to improve patients walking ability.

강재로 구속된 프리스트레스트 콘크리트 합성거더의 개발을 위한 실험연구

김정호,박경훈,황윤국,최영민,조효남,Kim, Jung Ho,Park, Kyung Hoon,Hwang, Yoon Koog,Choi, Young Min,Cho, Hyo Nam 한국강구조학회 2002 韓國鋼構造學會 論文集 Vol.10 No.3

콘크리트와 강판, PS강재의 구조적 장점을 극대화하여 낮은 형고로 경간을 장대화할 수 있는 새로운 교량 상부구조형식으로서 강재로 구속된 프리스트레스트 콘크리트 합성거더(Steel-Confined Prestressed Concrete Girder; SCP Girder)를 개발하였다. 개발된 SCP 합성거더의 효과적인 설계 및 제작을 위하여 설계프로그램을 개발하고 제작절차를 확립하였다. 이를 바탕으로 설계된 SCP 합성거더를 실물크기로 제작하여 시공성을 확인하였으며, 재하실험을 통해 설계의 타당성과 구조적 안전성 및 실적용 가능성에 대하여 검증하였다. A new type of bridge superstructures referred to as Steel-Confined Prestressed Concrete Girder (SCP Girder) was developed, which is composed of concrete, steel plate, and prestressing tendon. The girder may maximize structural advantages of these components; thus, long span bridges with low height girder may be constructed. For the effective design and fabrication of the gilder, the design software program was developed and the process of fabrication established. The experimental girder designed using the program was manufactured in actual size to confirm the fabric ability of the girder. Propriety of design, structural safety, and applicability of the gilder were verified through the load test.