水稻品種 “統一”과 “統一찰”의 遺傳背景 Ⅰ. Polyacrylamide gel 等電點電氣泳動에 의한 種子 esterase isozyme의 band 類型

Sun Zik Park(朴淳直),Hoon Kee Kim(金焄基) 한국육종학회 1990 한국육종학회지 Vol.22 No.3

Polyacrylamide gel isoelectric focusing was used to analyze the grain esterase isozymes of rice varieties, Tongil, Tongilchal and their related parents. Six major bands were revealed in the gel part of high pH and observed different banding patterns. Esterase isozyme banding patterns in grains were separated into three types, namely Est-Ⅰ, Est-Ⅱ and Est-Ⅲ in this experiment. Of the varieties analyzed, Japonica showed Est-Ⅰ and Indica was both Est-Ⅰ and Est-Ⅱ, while Japonica×Indica showed Est-Ⅲ, respectively. Esterase isozyme banding patterns of Tongil, Tongilchal, IR8, IR262, IR833, IR1317 and Peta were identical. It was concluded that the source of esterase isozymes genotype in grains of Tongil and Tongilchal was Indica variety Peta.

벼 雄性細胞 培養에 의한 trisomicsdml 移轉과 培養細胞의 再分化에 미치는 過剩染色體의 影響

Sun Zik Park(朴淳直),Gang Sup Lee(李鋼燮),Hee Jong Koh(高熙宗),Mun Hue Heu(許文會) 한국육종학회 1991 한국육종학회지 Vol.23 No.2

This study was carried out to investigate the transmission frequency of the extra chromosomes in rice trisomic plants through male and female gametes including selfed progeny, and to know the effect of extra chromosomes on the male gametic cell culture of rice. the extra chromosomes were transmitted to next generation at the rate of 0.0% to 9.7% through male gamete and 11.3% to 35.7% through female gamete, although the rate was 27.7% to 40.1% in selfed progeny of each trisomic type. Trisomic plant showed different culturability for callus induction, plant regeneration and green tissue appearence depending on the trisomic types in anther culture. Callus induction rates were ranged from 4.7% to 27.3% and plant regeneration rates 3.0% to 22.0%. In anther culture the highest transmission rate of extra chromosomes was 27.8% in trisomic 4(E), while the extra chromosomes of trisomic 12(A), 8(D) and 7(F) were not transmitted through anther culture in this study. The regenerated plants varied in chromosome numbers.

水稻 遺傳子 分析用 遺傳標識基株 育成 Ⅱ. japonica벼 遺傳檢定親으로서 三染色體植物의 育成

Sun Zik Park(朴淳直),Mun Hue Heu(許文會),Hee Jong Koh(高熙宗) 한국육종학회 1988 한국육종학회지 Vol.20 No.4

Eleven primary trisomics of the japonica group, developed and made available by lwata and Omura, were crossed as females with Korean bred Seolakbyo. From the F₁, F₂ and BC₁F₁ of each crosses 11 types, of trisomics were identified on the basis of their distinct morphological characters. Leaf character and plant type were useful criteria for the identification of A, E, F and O type trisomics, while B, C, D, G, H, L and N type trisomics were classified by the panicle and glume characters. The female transmission frequency of the extra chromosome of each trisomics ranged from 8.3 to 35% in F₁, from 16.7 to 50% in F₂ and from 14.3 to 50% in BC₁F₁, respectively in this study.

C3G색소 고함유 벼품종의 자유라디칼 소거작용 및 항염효과

박순직,류수노,Park, Sun-Zik,Ryu, Su-Noh 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

국내육성 흑자색미(흑진주벼, 흑남벼, C3GHi벼)와 일반미(일품벼, 추청벼, 동진벼) 품종의 메탄을 추출물을 대상으로 항산화 항혈전 항염증활성을 검토하였으며, 그 결과는 다음과 같다. 1. 0.4 mg/mL농도에서 C3GHi벼와 흑진주벼 추출물이 73.25%, 50.38%의 우수한 자유라디칼 소거능을 나타내었다. 2. 메탄올추출물의 혈액응고 저해활성을 측정한 결과, C3GHi 벼는 258.76%, 흑진주벼는 243.52%의 강한 트롬빈 저해활성을 보였다. 그러나 대조약물로 사용한 아스피린보다(398%) 약하게 나타났다. 3. 공시한 흑자색미와 일반미 품종들은 COX-1과 COX-2에 대한 활성이 나타나지 않았으나, C3GHi벼는 PD2에 대한 항염활성을 보였다. 4. H. pylori균에 의해서 나타나는 AGS cell line에 대한 독성을 조사한 결과 흑자색미 추출물은 $250{\mu}g$과 $500{\mu}g$ 처리군에서 모두 H. pylori균의 AGS cell line에 대한 부착을 억제하는 활성이 있는 것으로 판명되었으며 일반미 추출물은 H. pylori 균에 대한 저해작용은 없는 것으로 나타났다. 5. Zone assay에 의한 항균실험결과 흑자색미 추출물은 $200{\mu}g/mL$의 농도에서 ATCC 43504 및 COO1 균주에 대해서 clear zone이 검출되어 항균활성이 있는 것으로 판명되었으나 SEO 균주에 대해서는 항균활성이 검출되지 않았다. Free Radical Scavening and inflammatory of the methanol extracts, which were prepared from 6 difffrent bran of rice, were evaluated to investigate bioactive substances. Among them, the extract of C3GHibyeo and Heugjinjubyeo showed strong DPPH scavening activities (73.25% and 50.38% at 0.4 mg/ml, respectively). The extract of C3GHibyeo and Heugjinjubyeo showed strong thrombin inhibition activities (258.76% and 243.52% inhibition at 5 mg/ml, respectively). The result of antibacterial activity by Zone assay showed that C3GHi rice extract $(250{\mu}g\;and\;500{\mu}g)$ inhibited attachment of Helicobactor pylori on the ATCC48504 and COO1 cell line. But no effect on the SEO cell line. Cytotoxicity of blackish purple rice extract on the H. pylori doesn't showed. These result support a functional superiority of rice-base livelihood, and suggest that the development of healthy food using functional ingredients of rice is possible.

흑자색미의 C3G 색소함량 신속 예측모델 개발

류수노,양종진,박순직,Ryu Su-Noh,Yang Jong-Jin,Park Sun-Zik 한국작물학회 2005 한국작물학회지 Vol.50 No.suppl1

흑자색미의 C3C함량을 신속하게 분석하는데, FT-NIR을 이용한 C3G 함량 분석의 예측값과 HPLC 측정값의 정확도를 비교하였다. 1. FT-NIR을 이용한 C3G 함량 분석에서 사용된 시료는 별도의 전처리 과정없이 현미 상태 측정하여 HPLC 분석방법에 비하여 많은 시간과 비용을 아낄 수 있다. 2. 흑진주벼와 수원425호를 교배한 $F_{10}$ 385 계통을 사용하여 얻은 FT-NIR 검량식은 매우 높은 정상관을 보였다($R^2=0.943$, SEE=0.116). 이 검량식을 검증한 결과도 매우 높은 정상관을 보이고 실험오차도 매우 적어($R^2=0.928$, SEP=0.122) 측정정확도가 높게 평가되었다. 3. 본 연구의 결과, FT-NIR을 이용하여 비파괴적으로 신속하게 흑자색미의 C3G함량을 측정할 수 있게 되었다. 그리고 본 연구의 결과를 C3G함량이 높은 벼를 개발하는데 많은 양의 시료를 빠르게 분석할 수 있는 방법으로 이용할 수 있을 것이다. It has been reported that Cyanidin 3-Glu-coside (C3G) of the black pigmented rice was as the high anti-oxidency and analyzed by high performance liquid chromatography (HPLC). However, the analysis of C3G by HPLC is needed long pre-treated steps, so development of methods with simple pre-treated steps is needed in order to breed vices with high C3G contents. The analysis of components using near infrared reflectance (NIR) was well known as non pre-treated and nondestructive. C3G contents of Bengjinjubyeo$\times$Suwon425 $F_{10}$ 385 lines were used in order to develop C3G content prediction model in pigmented rice using FT-NIR. The results of C3G content of FT-NIR compared with HPLC were showed that the equation was f(x)=0.9427x+34.0430, $R^2$, standard error of calibration was 0.943, 0.116 and those of validation was 0.928, 0.122, respectively. This prediction model will be able to be used for analyzing C3G contents in black pigmented rice.

유색미 함유 Cyanidin 3-glucoside 정량분석

한상준,류수노,박순직,김홍열,Han Sang-Jun,Ryu Su-Noh,Park Sun-Zik,Kim Hong-Yeol 한국작물학회 2004 한국작물학회지 Vol.49 No.suppl1

Breeding of near-isogenic lines for resistance to bacterial blight, xanthomonas oryzae pv. oryzae, in rice

신문식(Mun Sik Shin),박순직(Sun Zik Park),신현탁(Hyun Tak Shin),이선용(Seon Yong Lee) 한국육종학회 1994 한국육종학회지 Vol.26 No.3

Twelve near-isogenic lines were selected from BC₃F₄ of three rice crosses between three different resistance-donor parents, Kogyoku(Xa-1), Rantai Emas(Xa-1, Xa-2) and Wase Aikoku 3 (Xa-3) and recurrent parent, Milyang 23, which is susceptible to bacterial blight. Several characters, such as diseases resistance, physiological traits, agronomic and grain characters were compared between Milyang 23 and selected breeding lines. Finally, HR11395-19-1(Xa-1), HR11396-3-3(Xa-1, Xa-2) and HR11397-28-1(Xa-3) lines were determined as near-isogenic lines(NILs) of Milyang 23 genetic background with different resistant gene to bacterial blight.

Synthesis time of storage proteins in rice endosperm

Byeong Gi Im(林炳琦),Sun Zik Park(朴淳直),Young Cheol Cho(趙英哲) 한국육종학회 1992 한국육종학회지 Vol.24 No.4

The proteins in rice endosperm were almost storage proteins which were composed with protein body type Ⅰ (PB-Ⅰ) and protein body type Ⅱ (PB-Ⅱ). PB-Ⅰ was composed mainly with prolamin, and PB-Ⅱ was glutelin and globulin. Investigation was on the synthesis time of the storage proteins in rice endosperm using by SDS-PAGE. The storage proteins of japonica variety ‘Kinmaze’ were synthesized from 9 days after flowering(DAF), and that of indica variety ‘IR 36’ did from 6 DAF. The endosperm mutants : esp-1, esp-2, and esp-3 that derived from ‘Kinmaze’ by MNU treatment showed not significant difference in the synthesis time of the storage proteins compared with ‘Kinmaze’. And PB-Ⅱ was synthesized faster than PB-Ⅰ in this investigation.

水稻品種 IR 50의 百葉枯病 抵抗性 遺傳子의 連關

Mun Sik Shin(申文植),Young Man Lee(李榮萬),Sun Zik Park(朴淳直) 한국육종학회 1988 한국육종학회지 Vol.20 No.1

In order to indentify linkage group of resistant gene(s) of rice variety IR50 bred at IRRI to Bacterial Blight, IR50 was crossed with five genetic marker lines and F₂ plants of these crosses were inoculated by the inoculum of Pathotype Ⅰ and Ⅲ, from maximum tillering stage to flowering stage. The resistance of IR50 to Pathotype Ⅰ and Ⅲ appeared to be controlled by different single dominant gene, respectively. These two genes were linked each other with recombination value of 1.0 - 13.1%, but they were independently segregated with all the marker genes tested, such as, C, lg, Ph⁺, g, gh, Lh, la, bc, and gl which located on the linkage group Ⅰ, Ⅱ, Ⅳ, Ⅵ, Ⅶ, Ⅷ, XI, and XII, respectively.

벼 分蘖開度의 遺傳

고희종(Hee Jong Koh),원용재(Yong Jae Won),차건완(Kean Wan Cha),이종철(Jong Cheol Lee),박순직(Sun Zik Park),허문회(Mun Hue Heu) 한국육종학회 1997 한국육종학회지 Vol.29 No.4

This study was carried out to investigate the inheritance mode of tillering angle using wx126-based near isogenic lines of different tillering angle, Broom, Open and Spread type, and the tillering angle of 73 rice varieties from Korea and IRRI was also estimated. The average tillering angle of rice varieties from Korea and IRRI were 10.5˚ (wide side) and 6.7˚ (narrow side) in japonicas, 12.3˚ (wide side) and 8.3˚ (narrow side) in Tongil type, and 8.7˚ (wide side) and 5.9˚ (narrow side) in Indicas. Average tillering angle at wide side of wx126 (original variety), Broom, Open, and Spread type at heading stage were 8.1˚, 5.5˚, 14.8˚, and 33.9˚, respectively, and the difference of tillering angle among the lines was maintained throughout the whole growth stage. In six F₂ populations of all-possible crosses among three near-isogenic lines, tillering angle showed continuous variation skewed to narrower parent, implying that it should be a quantitative character and the genes conferring the narrower tillering angle might be dominant over those conferring the wider tillering angle. Broad sense heritabilities for tillering angle varied 48.8% to 76.2%.