Nocodazole 투여에 따른 척수신경절세포 미세소관의 변화

박정현,이융창 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.2

사상과 울산자연집단내 노랑초파리 제2염색체의 유전적 변이성

박태동,최영현,이원호 부산대학교 환경문제 연구소 1987 環境硏究報 Vol.5 No.-

The genetic variabilities of second chromosome concealed in natural populations of Drosophila melanogaster have been analyzed annually. The collections have been continued three years since 1984 in Sasang and Ulsan natural populations and the flies were analyzed by Cy//Pm method and allelism test. The results were as follows: 1) The mean frequencies of deleterious gene(lethal plus semilethal), were estimated to be 30.60% in Sasang and 25.10% in Ulsan population. 2) The mean allelism rates between lethal genes isolated from natural populations were calculated to be about 1.66%, 1.64% in Sasang and Ulsan population, respectively, 3) The values of elimination were estimated by frequencies of deleterious genes and allelism rates, the mean values were 0.0017 in Sasang and 0.0010 in Ulsan population. 4) The frequencies of phenotypic sterility were ranged from 4.5% to 13.8% in two populatons and genetic sterility were showed from 3.43% to 4.65%, with the exception of Ulsan in 1986.

Analysis of Genetic Diversity of Korean Wheat Cultivars Using Microsatellite DNA Polymorphisms

Park,Yong-Jin,Cho,Gyu-Taek,Ma,Kyung-Ho,Lee,Sok-Young,Lee,Jung-Ro,Kim,Young-Chang,Cho,Eun-Gi,Kim Chang-Yung,Nam,Jung-Hyun,Rao,V,Ramanatha,Kang,Hee-Kyoung 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Genetic background and phylogenetic relationships among 20 Korean wheat cultivars were assessed using microsatellites after amplifying with 13 SSR primer pairs. Average allele number per primer pair was 3.36. Genetic similarities for every pair of cultivars ranged from 0.42 to 0.97, with 0.69 of overall average. Korean cultivars were divided into two major groups based on microsatellite DNA polymorphisms. Group I consisted of relatively old cultivars developed until 1970s, and group II contained the recent cultivars developed during 1980s and 1990s. Amongst old elite cultivars/lines, ‘Yukseung 3’, ‘Norin 12’ and ‘Norin 72’ contributed most to the genetic background of cultivars belonging to group I, and ‘Norin 4’, ‘Norin 12’, ‘Norin 43’ and ‘Norin 72’ to group II, respectively. The phylogenetic relationship of Korean wheat cultivars was in accordance with the genealogical data of each cultivar. The genetic background of each cultivar was assessed from the point of breeding and germplasm management such as variety identification and duplicated accessions for assisting in developing a system for the registration of new variety based on the molecular characterization in future.

케일 쥬스에 의한 AFLATOXIN B_1의 유전독성 억제 효과

최영현,박건영,이선미,유미애,이원호 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

The inhibitory effect of the fresh juice of kale on the genotoxicity induced by aflatoxin B_1 (AFB_1) in Salmonella and Drosophila was investigated. The kale juice had strong inhibitory activity for His^- to His^+ reverse-mutations induced by AFB_1 acting on S. typhimurium TA100. A similar inhibitory effect was detected in somatic cell mutation assaying system of D. melanogaster. Using the wing hairs spot test, we found that the formation of mutant hairs in adult flies as a result of feeding with AFB_1 in their larval stages was efficiently inhibited by coadministration of the fresh juice of kale, which revealed that it can inhibit gene mutation, deletion and mitotic chromosomal recombination. These results seem to suggest that kale juice may exert its inhibitory effect to mutagenic and/or carcinogenic properties of DNA damaging agents.

Drosophila melanogaster에 미치는 Kojic acid의 독성에 관한 연구

최영현,박연규,이원호 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

수종의 Aspergillus 및 Pencillum 속 균에 의해 생산되는 진균애사 산물인 kojic acid (5-hydroxy-2-hydroxymethyl-γ-pyrone) 가 Drosophila melanogast에 미치는 독성 효과에 대한 기초 조사를 실시하였다. Kojic acid의 처리 농도가 증가할수록 발생단계상의 발육속도의 지연과 성체에 대한 높은 치사율의 증로 강한 독성을 나타내었다. 성체에서의 LC_50 값(the concentration at which 50% of treated filles died within 72hr)은 약 4.8mg/ml로 추정되었으며, 발생단계상의 처리로 인한 우화 후 성비에 대한 유의적인 영향은 주지 않았다. 그리고 본 실험에서 사용한 attached-X법에 의한 반성 치사 돌연변이 유발에서도 유의적인 돌연변이 유발 효과가 없는 것으로 나타났다. The present experiment was carried out to detect the basic toxic effect of kojic acid (5-hydroxy-2-hydroxymethyl-γ-pyrone), a fungal metabolite produced by some species of Aspergillus and Penicillium, in Drosophila melanogaster, Kojic acid was highly toxic on the developmental and adult stages, resulting in prolongation of the developmental time and lowering of the viability from larvae to adult, and high mortality of adults as dose 'increased, The LC_50 value at 72 hr i.e., the concentration at which 50% of treated flies died within 72hr, was about 4.8mg/ml. As to the sex ratio of flies fed kojic acid during the development, there was no differences between the control and the treated groups, And, kojic acid gave no a significant mutagenic effect on the inducbon of the sex-@ed lethal mutagenesis by the attached-X method used in this study.

전산모형과 지리정보시스템을 결합한 Wed 기반의 유역 및 하수도 관리시스템의 개발

김준현,박형춘,한영한 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A

A Web based watershed and sewer management system was developed for the analysis of stormwater runoff and sewer flow, and for optimal operation of sewer works using ArcView and SWMM. SWMM and ArcView were dynamically linked together using Avenue and Visual Basic in order to construct user-friendly management system. The developed system was applied to Choonchun city to verify its utilities. All the relevant field data were analyzed on the basis of developed system, and the modeling of runoff and sewer flow was implemented using RUNOFF and TRANSPORT blocks in SWMM. This system was connected to the management system of surface and subsurface environment management system in order to develop an integrated environmental mangament system. Futhermore. this system will be a critical part of overall control system of sewer works including sewer line and wastewater treatment plant. As this system can provide comprehensive prediction of flow and pollution profiles and analytical tool equipped with Web-GIS, it could serve widely as a tool not only for optimal mangament, but also for decision support system to examine the efficiency of planning and implementation of sewer projects.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

오차항의 분산이 이분산일 때의 캘리브레이션에 관하여

김응환,박석윤,박래현 충남대학교 기초과학연구소 1997 忠南科學硏究誌 Vol.24 No.2

This thesis suggests an estimation procedure in the simple linear calibration model when the variation of error term are propotional to the independent variables. And it is proved that we can obtain the finite closed confidence interval of unknown controlled variable under the condition that the null hypothesis of zero slope is rejected.

보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip

서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1

Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).

上顎洞에 發生한 Aspergillosis의 一例報告

林大銑,宋圭鉉,朴敬浩,朴暎緖,李玩永 中央醫學社 1963 中央醫學 Vol.5 No.4

A case of secondary aspergillosis of the left maxillary sinus following chronic sinusitis in a 42 year old male coal miner specialist is reported. His chief complaints were nasal obstruction and postnasal drip of several years duration. Roentgenogram revealed marked and moderate shadow in the left and right maxillary sinuses respectively. Significant operative findings included almost paper-thin bony walls of the left maxillary sinus, and small amount of yellow greenish purulent material was drained, followed by yellowish brown semisolid tissue on further curettage. The latter specimen was found to be essentially mass of fungi, showing numerous distinctly septate, branching hyphae on the Papanicolaou stain of the direct smears of the cut surface of the specimen, and Hematoxylin-Eosin and Periodic Acid Schiff stains of paraffin sections. Pertinent aspects of clinical and pathological differential diagnosis between aspergillosis - and mucormycosis, are described. Aspergillosis presents usually benign course, with insignificant chief complaints as our case, whereas mucormycosis is most frequently associated with uncontrollable diabetics, and run usually acutely fatal course. There are three main types of ucormycosis-cerebral, pulmonary and intestinal. The characteristic broad nonseptate branching hyphae of mucor, and marked affinity to the blood vessels,, penetrating the tough walls of arteries, with thromboses and infarction, readily differentiate aspergillosis from mucormycosis.