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Lim, Wang-Jin,Choi, Kyung-Min,Hwang, Se Young 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4
A novel system has been developed to produce δ-aminolevulinic acid (ALA) using the intact cells of late logarithmic phase of Rhodocyclus gelatinosus KUP-74. The system was shown optimum yield of extracellular ALA under a condition of anaerobic light irradiation (4 Klux) at 30℃ with no variation in cell mass. The rate of extracellular ALA formation was stimulated by low doses of either C_4 or C_5 ALA biosynthetic precursors, where 5 mM (C_4) and 3 mM (C_5) of each precursors were appeared to generate the maximum rates of 3.3 and 4.0 nmoles of ALA per 0.35 ㎎ cells per hr, respectively. Half-life of the system was 10 hr in a sense of an ability of portage transport of L-glutamate, and sequential dose of this compound was resulted in promising recovery of the ALA.
Wang, Guanlin,Lim, Do-Seon,Choi, Baik-Dong,Park, Jin-Ju,Jeong, Soon-Jeong,Kim, Jin-Soo,Kim, Jae-Duk,Park, Jung-Su,Kim, Eung-Kwon,Kim, Byung-Hoon,Ham, Joo-Hyun,Jeong, Moon-Jin The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.2
Secretory leukocyte protease inhibitor (SLPI) plays an important role in promoting the invasion and metastasis of a range of cancer cells. However, there are no reports of the expression and function of SLPI in oral carcinoma cells. In this study, the oral carcinoma cell line KB was used to determine whether SLPI affects the proliferation, migration and invasion of oral carcinoma cells. RT-PCR and Western blotting revealed high levels of endogenous SLPI expression in KB cells as well as a strong increase in SLPI secretion after wounding compared to immortalized normal oral keratinocytes (INOK). The wound healing assay revealed more migration of KB cells than INOK cells, and the SLPI treatment increased the migration of KB cells. KB cell proliferation was increased significantly by the SLPI protein but decreased by SLPI-siRNA. SLPI strongly increased the migration and invasion of KB cells. On the other hand, SLPI-siRNA decreased the migration and invasion of KB cells. This suggests that SLPI plays an important role in the metastasis of oral carcinoma cells.
Lim, Wang Jin 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.4
Six stains of methylotrophic yeast were examined for production of L-methionine-enriched cells. Candida boidinii (Kloeckera sp.) No. 2201, which accumulated 0.54 ㎎/g-dry cell weight (DCW) of free L-methionine (pool methionine), was selected as the parental strain for breeding L-methionine-enriched mutants. Ethionine-resistant mutants derived from the strain by W irradiation. A mutant strain, E500-78, which was resistant to 500㎍/㎖ of DL-ethionine, accumulated 6.02㎎/g-DCW of pool methionine. The culture conditions for mutannt strain E500-78 to increase pool methionine accumulation were optimized. The mutant strain accumulated 8.80 ㎎/g-DCW of pool methionine and contained 16.02 ㎎/g-DCW total methionine. L-methionine-enriched cells production of mutant strain E50078 was greatly improved by the control of pH and by feeding of methanol and other medium components during cultivation in a jar fermentor. Under the optimal conditions, 38.5g (as dry weight) of cells and 282 ㎎ of pool methionine per ℓ of culture broth were obtained after 11 days of cultivation. With limited methanol in continuous cultivation, pool methionine productivity reached a maximum value of 1.14 ㎎/ℓ/h at a dilution rate of 0.05 h^(-1). The effects of an ethionine-resistant mutation in a methylotrophic yeast, Candida boidinii, were studied. In mutant strain E500-78 (ethionine-resistant), SAM synthetase activity was low and was only slightly repressed by L-methionine. Formyltetrahydrofolate synthetase and serine hydroxymethyltransferase were involved in synthesis of the methyl group of L-methionine. The activities of the methyl group transferring enzymes and homocysteine transmethylation were repressed by L-methionine in the wild type strain, but not in the mutant. The activities of the methyl group transferring enzymes were markedly stimulated when the mutant was grown in methanol medium.
Analysis of Ploidy Levels of Korean Wild Asteraceae Species Using Chromosome Counting
Wang Yan,Jin Hee Lim,Jae A Jung,Won Hee Kim,Ki-Byung Lim,Raisa Aone M. Cabahug,Yoon-Jung Hwang 한국화훼학회 2019 화훼연구 Vol.27 No.4
Because of their attractive and colorful flowers, many species from the genus Aster serve as garden plants. Chrysanthemum owes its popularity to its ornamental and medicinal herb value. It can be used as a cut flower, potted plant, vegetable, and herbal tea. Plant breeders have attempted to identify the available species and produce new cultivars to improve the quality of chrysanthemum for commercial purposes. The use of cytogenetic studies has paved the way for identifying compatibility, ancestry, and other useful information for this undertaking. Thus, an investigation was conducted into the chromosome numbers of 23 wild Asteraceae species in Republic of Korea to determine their genetic characteristics and variations. The somatic chromosome spread has been used for chromosome counting. The results revealed that Asteraceae species have a chromosome range from 18 (diploid) to 54 (hexaploid). These findings provide primary and important information on the chromosome numbers in chrysanthemum plants that can be used to select the right variety for cultivation.
Lim, Yun-Sung,Lee, Jin-Choon,Lee, Yoon Se,Lee, Byung-Joo,Wang, Soo-Geun Korean Society of Otorhinolaryngology-Head and Nec 2012 Clinical and Experimental Otorhinolaryngology Vol.5 No.2
<P><B>Objectives</B></P><P>Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. However, the effect of human MSCs on the growth of human tumors is not well understood. The purpose of this study is to confirm the growth effect of palatine tonsil-derived MSCs (TD-MSCs) on head and neck squamous cell carcinoma (HNSCC) cell lines and to elucidate the mechanism of their action.</P><P><B>Methods</B></P><P>TD-MSCs were isolated from patient with chronic tonsillitis and tonsillar hypertrophy. Two human HNSCC cell lines (PNUH-12 and SNU-899) were studied and cocultured with isolated palatine tonsil-derived MSC. The growth inhibitory effect of MSCs on HNSCC cell lines was tested through methylthiazolyldiphenyl-tetrazolium (MTT) assay. The apoptosis induction effect of MSCs on cell lines was assessed with flow cytometry and reverse transcriptase (RT)-PCR.</P><P><B>Results</B></P><P>Palatine tonsil-derived MSCs exhibited a growth inhibitory effect on both cell lines. Cell cycle analysis showed an accumulation of tumor cells predominantly in G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs.</P><P><B>Conclusion</B></P><P>TD-MSCs have a potential growth inhibitory effect on HNSCC cell lines <I>in vitro</I> by inducing apoptotic cell death and G1 phase arrest of cell lines.</P>
왕희정,백인욱,유석진,오미혜,서연림,장석효,이혁상 인제대학교 1994 仁濟醫學 Vol.15 No.1
유암예후에 영향을 미치는 전통적 3인자로 종양의 크기, 전이액와임파절의 수 및 menopausal status를 들 수 있고, 이 외에 최근에는 면역학과 분자생물학의 비약적 발달로 호르몬 수용체, DNA ploidy, cathepsin D, pS2 protein 및 다양한 oncogene들의 증폭이나 과발현 등이 새로운 독립적 예 후인자로 보고되고 있다. 저자들은 유암 110례의 10년 추적조사 성적을 기반으로 이들 새로운 예후인자중 c-erbB-2 oncoprotein, cathepsin D 및 pS2 protein의 과발현이 전통적 예후인자들과 상관성이 있는지와 독립적 예후인자로서 의의가 있는지를 확인하고자 본 연구에 착수하였다. This study was undertaken to define the prognostic value of the overexpression of c-erbB-2 oncoprotein, cathepsin D and pS2 protein in 40 breast cancer patients. The results were as follows : 1.Overall, 32% of patients were positive for c-erbB-2 oncoprotein. Positive staining correlated with menopause status, tumor size and TNM staging, but not wish axllary nodal status. Although there was apparent difference in cumulative survival rates between positive-and negative-staining groups, it was not statistically significant(p=0.0625). 2.52.5% of patients were positive for cathepsin D. Positive staining did not correlate with menopause status, axillary nodal status or tumor size. There was no statistical difference in cumulative survival rates between positive-and negative-staining groups of cathepsin D. 3.57.5% of patients were positive for pS2 protein. Positive staining did not correlate with menopause status, axillary nodal status, tumor size or tumor stage. There was no statistical difference in cumulative survival rates between positive-and negative-staining groups of pS2 protein.
Controlled Folding of Single Crystal Graphene
Wang, Bin,Huang, Ming,Kim, Na Yeon,Cunning, Benjamin V.,Huang, Yuan,Qu, Deshun,Chen, Xianjue,Jin, Sunghwan,Biswal, Mandakini,Zhang, Xu,Lee, Sun Hwa,Lim, Hyunseob,Yoo, Won Jong,Lee, Zonghoon,Ruoff, Rod American Chemical Society 2017 Nano letters Vol.17 No.3
<P>Folded graphene in which two layers are stacked with a twist angle between them has been predicted to exhibit unique electronic, thermal, and magnetic properties. We report the folding of a single crystal monolayer graphene film grown on a Cu(111) substrate by using a tailored substrate having a hydrophobic region and a hydrophilic region. Controlled film delamination from the hydrophilic region was used to prepare macroscopic folded graphene with good uniformity on the millimeter scale. This process was used to create many folded sheets each with a defined twist angle between the two sheets. By identifying the original lattice orientation of the monolayer graphene on Cu foil, or establishing the relation between the fold angle and twist angle, this folding technique allows for the preparation of twisted bilayer graphene films with defined stacking orientations and may also be extended to create folded structures of other two-dimensional nanomaterials.</P>