Cyclosporin A 가 Polyvinylpyrroridone에 對한 마우스의 免疫反應에 미치는 影響

河大有,金哲基 대한화학요법학회 1984 대한화학요법학회지 Vol.2 No.1

Cyclosporia A(CSA)가 胸腺依存性 抗原인 緬羊赤血球와 胸腺非依存性 第2型 抗原인 polyvinylpyrroridone(PVP)에 對한 免疫反에 미치는 影響을 알아보고자 本 實驗을 實施하였다. CSA 處理는 緬羊赤血球와 PVP에 對한 plaque forming cell (PFC) 反應을 抑制하였다. 抗緬羊細胞血淸의 投與는 PVP에 對한 PFC 反應을 顯著히 亢進시켰다. 이와같은 實驗結果는 CSA가 胸腺依存性 抗原과 胸腺非依存性 第2壟 抗原에래 對한 免疫反應을 抑制하며 superessor T cell이 正常的으로 PVP에 對한 抗體反應을 抑制하고 있음을 示唆한다. This study was undertaken to assess the effect of cyclosporin A(CSA) on immune response to thymus-dependent antigen, sheep red blood cells(SRBC) and thymus independent type 2 antigen, pol~inylpSrrorjdone(P~p~. G A treatment was done by 3 subcutaneous injections. of CSA(100 mg/kg) on oae day before, at the same time of, and one day after immunization. Treatment of mice with C5A caused the profound suppression of plaque-forming cea(PFC) responses to S B C and PVP. Intravenous treatment of PW-Immunized mice with.antithjmocfie serum caused the profound enhancement of PFC response to PVP. These results indicate that CSA inhibit both thymusdependent and thymus-independent type 2 antigen and suppressor T cell3 normally function to dampen the immune response to PVP.

簡單한 細菌學的 方法을 利用한 尿中 抗菌物質 檢索과 同定

河大有,李黃洙,金哲基 대한화학요법학회 1986 대한화학요법학회지 Vol.4 No.2

요(尿)와 혈청에서 항균물질을 검색하고 동정할 수 있는 간단한 세균학적 방법을 확인하고 확대시켰다. 이 간단한 방법을 이용하여 544가검요(可檢尿)를 검사하였든 바 이들로부터의 항생제 검출율은 13.6%(74가검물)이었다. 15가검물에서 항균제를 동정하였다. 이 검사방법은 신속하게 실시할 수 있고, 값이 저렴하고, 경험이 없는 사람도 실시할 수 있기 때문에 일상검사에 이 방법을 사용할 것을 제안하였다. A simple bacteriological method to detect and identify antibacterial substances in urine and serum were confirmed and extended. Employing this simple method, we have found that of 544 urine specimens from various sources, the frequency of finding antibiotics in urine specimens was 13.6%(74 specimens). The antibiotics was identified confidently with typical patterns of inhibitory zone in 15 urine specimens. As this method is neither time consuming nor expensive and can be set up by inexperienced laboratory personnel, we would like to propose this method to be used in routine laboratories.

Heligmosomoides polygyrus 감염 마우스의 비장세포로 부터 만든 Conditioned Supernatant의 면역억제작용

하대유,한병갑,김명선,고유승 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

Heligmosomoides polygyrus is gastrointestinal parasitic nematoda which is common parasire of wild rodents. The experimental infection with this parasite has been studied extensively in models of host-parasite interaction. The present study was undertaken to investigate both the effects of administration of conditioned supernatant or conditioned medium(CM) prepared from H. polygyrus-infected mice on the humoral and cellular immune responses in mice. Normal conditioned supernatant(NCM) was prepared from uninfected mouse splenocytes stimulated with Con A. Supematants conditioned by Con A-stimulated splenocytes of H. polygyrus -infected mice were prepared on different days post-infection, namely on day 6(HCM-D6), day 14 (HCMD14) and day 18 (HCM-D18) post-infection with H. polygyrus L3 larvae. Effects of NCM, HCM-D6, HCM-D14 and HCM-D18 on delayed-type hypersensitivity(DTH) to sheep red blood cells (SRBC), contact hypersensitivity to dinitrofluorobenzene (DNFB), hemagglutinin response to SRBC, ovalbumin (OVA)-induced active systemic anaphylaxis(ASA), and anti-OVA specific IgE were investigated. Effect of anti-IL-4 antibody (11B11) on immunoinhibitory action of HCMD18 in OVA-induced ASA was also investigated. It was found that the administration into mice of HCM-D6, HCM-D14 or HCM-D18 significantly suppressed DTH to SRBC, contact hypersensitivity to DNFB, hemagglutinin response compared with NCM. The degree of immunosuppressive activity of HCM was less marked in HCM-D6 than HCM-D14 and HCM-D18. Interestingly, HCM-D18 prepared from ICR mouse strain also showed the profound suppression of OVA-induced ASA in BALB/c and C57BL/6 mouse strains as well as in ICR mice. ASA-inhibitory activity of HCM-D18 was Somewhat abrogated in terms of mouse mortality when mice were treated in the combination of HCM-D18 and anti-IL-4 antibody, indicating that IL-4 may play a role, at least in part, in the inhibitory activity of HCM. Taken together, the present study may be the first to demonstrate that conditioned supernatants prepared from the spleen cells of H. polygyrus-infected mice may suppress the in vivo humoral and cellular immyne responses to heterologous antigens, particularly fatal anaphylaxis induced by OVA, strongly suggesting that

癌患者의 腹水 및 肋膜渗出液이 健康人 淋巴球의 Rosette形成에 미치는 影響

河大有,鄭憲鐸 大韓免疫學會 1979 大韓免疫學會誌 Vol.1 No.1

Non-specific suppressions of delayed-type hypersensitivities (DTH) measured by skin reactivity to certain antigens, response of lymphocytes to mitogens and spontaneous(E) rosette formation of lymphocytes with sheep red blood cells (SRBC) have been reported to occur in cancer bearing-patients. The mechanisms responsible for these immunosuppressions remain unclear, but some investigators suggested that the immunosuppressions may result from immunosuppressive factors exudated from tumor masses. This study was undertaken to evaluate the effect of ascitic and pleural fluids from patients with cancers metastatic to peritonium or pleura. Non-cancerous ascitic and pleural effusions were used as controls. The ascitic and pleural fluids from hepatoma patients and pulmonary carcinoma patients decreased not only the percentages of early and late rosette formations severely but also the affinity of lymphocytes to SRBC. On the other hand ascitic fluids from stomach cancer patients increased the percentages of early rosette formation and the affinity of lymphocytes to SRBC. Control effusions exerted no effect on rosette formation except the pleural effusions from pulmonary tuberculosis patients. Pleural effusions obtained from tuberculosis patients slightly increased the percentages of rosette formation and the affinity of lymphocytes to SRBC.

抗生劑耐性傳達因子의 除去에 關한 實驗的 硏究

河大有,金炯魯,金圭次,李憲九 대한의학협회 1979 대한의학협회지 Vol.22 No.9

精神分裂症 患者와 正常人의 胸腺細胞에 對한 抗體

河大有,慇洪培 中央醫學社 1976 中央醫學 Vol.30 No.1

Purpose of this study is to investigate the awareness on the disabled man by means of the semantic differential method.Subjects are sixty eight hospitalized disabled men and forty three nurses in National veterans Hospital from Dec. 1 to 3, 1975. Distance between two counter meaning on one concept is divided to seven scale and grade of the nurses and the disabled men is compared in each concept, in the aspect of evaluation, potency, activity. Obtained data is analyzed by mean and critical ratio and semantic space is figured out. The result is as follows: � In the aspect of evaluation, mean of the nurse'sopinion on the disabled man (3.60) is lower than that of the disabled men (4. 54) at significant level (P<.01) ? In the aspect of potency, mean of the nurses' opinion on the disabled man (3.71) is lower than that of the disabled men (4.53) at significant level(P<. 01) ? in the aspect of activity, mean of the nurses' opinion on the disabled man (3.57) is lower than that of the disabled men (4. 28) at significant level (P<0.05) ? In awareness on the disabled man, the level of the disabled men is higher and more positive than the nurses.

Heligmosomoides polygyrus 감염 마우스의 면역반응 잠재력

하대유,고유승,한병갑 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.2

Heligmosomoides polygyrus(=Nematospiroides dubis) is gastrointestinal parasitic nematoda which is common parasite of wild rodents in North America, Western Europe and the CIS. The experimental infection with this parasite has been studied extensively in models of host-parasite interaction. The present study was undertaken to investigate the effect of H. polygyrus infection on the humoral and cellular immune responses in mice. It was found that H. polygyrus infection suppressed delayed-type hypersensitivity(DTH) to SRBC, contact hypersensitivity to dinitroflurobenzene (DNFB) and hemagglutinin response to SRBC. Surprisingly, H. polygyrus infection suppressed active systemic anaphylaxis(ASA) induced by ovalbumin(OVA), but elicited marked increase of the total IgE antibody and total IgG production compared with uninfected mice. In a histological examination of the mucosal mast cell(MMC) response in vivo in ICR mice, H. polygyrus infection elicited a enhance MMC response 14 days post-infection, but did not show any difference 21 days post-infection, but did not show any difference 21 days post-infection compared with uninfected controls, suggesting mucosal mastocytosis response may depend on certain stage of life cycle of H. polygyrus. Taken together, the present study strongly suggested that H. polygyrus infection in mice in-creased the total IgE and IgG antibody production, but suppressed cellular immune response to SRBC and DNFB, hemagglutinin respose and OVA-induced anaphylaxis, and that mucosal mastocytosis may be decreased or increased depending on certain stage of life cycle H. polygyrus.

Dexamethasone이 마우스의 여러 가지 Suppressor T Cells에 미치는 영향

하대유,김형일,임선영 大韓免疫學會 1987 大韓免疫學會誌 Vol.9 No.1

The effect of dexamethasone(DEX) on delayed-type hypersensitivity(DTH) reactions and antibody responses to various concentrations of sheep red blood cells (SRBC), and of polyvinylpyrroridone(PVP), graft-vs-host reaction were evaluated. DEX treatment(lOOpg/mouse, 1.p, b.I.d.) was begun 1 daybefore immunization or cell transfer and continued until challenge with antigen or throughout experiments. The treatment with DEX inhibited DTH and antibody responses to high, low and intermittent doses and markedly inhibited GVH reactivity of thymus, lymph node and spleen cells. DEX markedly inhibited antibody response to high(25pg/mouse) and low(0.025pg/mouse) doses of PVP, but not to intermit-tent dose(0.25pg/mouse) which is optimal immunizing dose of PVP in mice. The ability of DEX to inhibit the immune suppression mediated by different types of murine T sup-pressor cell was also evaluated. The activity of suppressor cells induced by UVB irradiation, :: dinitrobenzene sulfonic acid(DNBS) injection and inoculation with a high dose of SRBC was evaluated after adoptive transfer to naive syngeneic recipients. DEX was administered to recipients b. i. d. at the dose of 100pg/mouse, beginning I day before the adoptive transfer of UVB―, DNBS― or .SRBC―tolerized spleen cells (1 X10'/mouse) continuing until challenge. DEX treatment abrogated the expression of DNBS―induced, Lyt―2 , T suppressor cells and stimulated contact sensitivity to dinitrofluorobenzene(DNFB) in adoptive transfer experiments. In contrast, the Lyt―1'' T suppressor cell activity induced by UVB irradiation and the Lyt―2+ T-suppressor cell-mediated unresponsiveness induced by inoculation with a high dose of SRBC were refractory to DEX treatment. UVB-induced suppression of both con-tact sensitivity to DNFB and DTH response to SRBC could be transferred into recipients. These results indicate that T suppressor cell populations differ markedly in their susceptibility to modulation by DEX treatment, illustrating the diversity of suppressor cell mechanisms operating different in immune states. This also suggests that meaningful application of immunotherapeutic modulation may re-quire a precise understanding of the property of suppressor cell suhpopulations involved in a specific immune response before reliable ther apy can be achieved.

人蔘에 關한 細菌學 및 免疫學的 硏究 : 第III報. 人蔘이 Mouse의 免疫反應에 미치는 影響 III. Effect of Korean Panax ginseng on Immune Response in Mice

河大有,李正鎬 大韓免疫學會 1979 大韓免疫學會誌 Vol.1 No.1

The prcesent study was undertaken to evaluate the effects of the Korean Panax ginseng on the immune response in mice. One-half milligram of ginseng extract in 0.25ml volume was given to ICR mice intraperitoneally for 14 days. The ginseng treated and control mice were challenged with sheep red blood cells (SRBC) by intradermal injection 47days after sensitization with varying doses of SRBC by intravenous injection: Such immune responses as delayed-type hypersensitivity (DTH), antibody formation and rosette forming cells with SRBC were measured. DTH of ginseng treated mice were generally increased than those of control mice. In both groups, the peak responses were shown in the mice sensitized with 1O7SRBC. Rosettespleen cells of ginseng-treated mice were somewhat increased than those of control mice, and the degree of increment was dependent on the dosage of sensitizing SRBC. The serum antbody titres were generally decreased in the ginseng treated mice when compared with the control mice. And the titres were shown to be dependent on the antigen doses used for sensitization. These findings suggest that the Korean ginseng enhances cell-mediated immune response, but depresses the humoral immune response.

人蔘에 關한 細菌學 및 免疫學的 硏究 : 第1報, 人蔘이 細菌增殖에 미치는 影響 Ⅰ. Effect of Panax ginseng on the Bacterial Growth

河大有,李正鎬 全北大學校 1976 論文集 Vol.18 No.-

The present study was undertaken to assess the effect of the Korean Panax ginseng extract on the growth of various bacteria, such as S. typhi H_901, S. typhi O_901, S. typhimurium, E. coli J-5, E.coli K-12, E. coli 0-17, Shigella flexneri, Staph. aureus and Listeria monocytogenes. The culture media employed in this experiment were minimum medium broth (MM), nutrient broth (NB) and brain heart infusion broth (BHIB). The growth response of bacteria to the various concentrations (1 mg/ml or 10 mg/ml) of ginseng extract was determined as a function of time by the measurement of a broth culture in a spectrophotometer at 635nm. The growth of all the strains of bacteria except Staph. aureus was enhanced in MM by the addition of ginseng extract in concentration of 1 mg/ml, 5 mg/ml or 10 mg/ml. Among the bacteria, particulary, the growth of E. coli was prominently increased. The growth of Salmonella in NB was somewhat increased when NB was added with ginseng extract in concentration of 1 mg/ml, or 5 mg/ml, whereas the growth of Salmonella, especially S. typhimurium was remarkably inhibited in the presence of 10 mg/ml of ginseng extract. The growth of all the strains of E. coli examined was enhanced in NB by the addition of ginseng extract in concentration of 1 mg/ml, 5 mg/ml or 10 mg/ml, and the degree of enhancement varied from strains to strains depending on the concentration of ginseng extract added. The growth of Staph. aureus was suppressed until 24 hours after the begining of culture when 5 mg/ml or 10 mg/ml of ginseng were added into nutrient broth. However, the growth was more or less increased after 24 hours' incubation. The growth of Shigella flexneri in NB was not influenced by the addition of ginseng extract into NB in concentration of 1 mg/ml or 5 mg/ml. On the contrary, the growth was considerably inhibited when 10 mg/ml of ginseng extract were added into NB. No particular effect was observed in the growth of Listeria monocytogenes when 1 mg/ml of ginseng extract was added into BHIB. However, when 5 mg/ml or 10 mg/ml of ginseng extract was added the growth was slightly decreased until 12 to 20 hours after the begining of culture, while the growth was increased after 24 hous' incubation. The lag phase of bacterial growth was usually prolonged in media containing 10 mg/ml of ginseng extract.