Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

또 하나의 냉전

박소현(Park So-Hyun) 현대미술사학회 2006 현대미술사연구 Vol.19 No.-

In 1970, the so-called 'revisionists' of the United States criticised that 'the triumph of American art' was the product of 'cultural Cold War' and Abstract Expressionism as well as the Museum of Modern Art in New York were the weapons of Cold War. Their criticism suggested the problematic issues about modern art in Cold War, but they had some logical limits in their literatures: on the one hand, they were almost interested in the centre of modern art, Paris or New York, and on the other hand, they believed the 'centre', understood as American art in New York after World War Ⅱ, was getting to be diffused into the other areas step by step, for example, Europe in the 1950s, Asia in the 1960s. This means even the critical 'revisionists', probably unconsciously, were not free from and consequently reproduced the geopolitics of the 'centre' with regard to modern art. As for Japan, however, the cultural Cold War' did not come late in the 1960s, but started from the Occupation of the GHQ (General Headquarters/Supreme Commander for Allied Powers) in 1945. And although under the Occupation, the meaning of modern art in Japan was not directly connected to 'the triumph of American art', rather the exhibitions of French modern art were vigorously held. These exhibitions were taken place almost by the newborn 'Kokuritsuhakubutsukan (National Museum)' which had been the property of Tenno (Emperor of Japan) but was reconstituted as that of people by the GHQ on the basis of the Occupational ideology of 'democracy' after the defeat of war. In short, there appeared the 'cultural Cold War' in Japan during the Occupation years in the form of 'democratic modern' French art. And more, the ideology of 'democracy' was so discussive, inconsistent and changing one that could not say that the ever-determined principle of the 'centre' was received as it was without any resistance or modification in Japan. Therefore, I think the case of Japan under the Occupation is a good one to question about the geopolitics of 'centre' reproduced by the 'revisionists'. During the Occupation years, the meaning of 'democracy' and 'democratic modern' art was changed gradually, from anti-militarism (or anti-nationalism) to anti-communism (or anti-socialism) and ended up to the resurrection of Japanese nationalism. With these changes, the French artists or artistic movements was selectively accentuated, from Impressionists to Fauvists or Cubists and to decorative Fauvists, especially Matisse. First, right after the defeat of war, because the Occupational principle was centered on the anti-militarism, 'democracy' was interpreted as a broad one, including socialism, and so there happened a series of competitions for which one is more 'democratic' art between Impressionist realism and socialist realism. But with the opening of 'Kokuritsuhakubutsukan' in 1947, in the second place, the meaning of 'democracy' in that institution was changed into anti-communism, and at the same time 'the autonomy of art(art for art's sake)' was underlined as the institutional principle. On the behalf of this principle, art criticisms and artistic discourses also put it that Fauvists or Cubists was more developed ones than Impressionists because the first was closer to 'the autonomy of art' than the last. This interpretation was conscious of the formulation of modern art by Museum of Modern Art in New York regarded as 'center of art world' and the extraordinary Museum of Modern Art was open within the Kokuritsuhakubutsukan in 1948. And then, from 1949 to 1951, the third aspect of these changes, The GHQ decided to make Japan anti-communist base in Asia, and for the purpose of it to make Japan an independent nation-state was needed. So the Kokuritsuhakubutsukan made a great point of worldliness of Japanese art, and emphasized the superiority of Japanese classics or traditional culture which had been regarded to influence French modern arts. This was the return of Japonism which ha

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

서산지역의 대기질 경년변화 특성에 관한 연구

손병현(Shon Byung-Hyun),박동소(Park, Tong-So) 한국산학기술학회 2008 한국산학기술학회논문지 Vol.9 No.3

본 연구에서는 1998년부터 2006년까지 9년 동안의 서산 지역 대기오염물질 농도 변화 특성을 조사하였다. 조사 결과, 최근 3년 동안 CO와 O₃을 제외한 SO₂, NO₂ 및 PM-10은 농도 변화가 거의 없는 안정화 단계를 보였다. NO₂, CO 및 SO₂의 월평균농도 변화는 여름철에 감소하다 겨울철에 증가하는 경향을 보였으며 O₃과 PM-10은 봄철에 높은 농도를 보이며, 강수가 집중되는 여름철에 가장 낮은 농도분포를 보였다. 2006년 12월까지 오존의 1시간 기준치인 0.1 ppm을 초과한 횟수는 동문동이 68회, 독꽂리 93회였고 8시간 기준치얀 0.06 ppm을 초과한 횟수는 동문동 210 회, 독곶리 213회였다. 또한 미세먼지의 경우 24시간기준인 150 ㎍/㎥을 초과한 횟수가 동문동 25회, 독곶리 54회로 나타났다. This study was performed to evaluate the characteristics of current air quality and to examine the monthly and yearly variation of SO₂, O₃, CO, NO₂, and fine particulate matter(PM-10) concentration in Seosan for the period of 1998-2006. During the recent 3 years, from 2004 to 2006, the yearly mean concentration of SO₂, NO₂, and PM-10 remained stability but CO and O₃ are gradually decreased. The monthly mean concentration of NO₂, CO, and SO₂ were high in the winter season and decreased steadily to the summer season, but the maximum concentration of O₃ and PM-10 appeared in the springtime. The number of days exceeding 24-hour standards for PM-10 were 54 times at Dokgodri and 25 times at Dongmundong, The number of sampling days exceeding the l-hour and 8-hour standards for O₃ were 68 and 210 times at Dongrmundong and at Dokgotri 93 and 213 times, respectively.

바우하우스의 기계인간

박소현(So-Hyun Park) 현대미술사학회 2003 현대미술사연구 Vol.15 No.-

This thesis started from the question of the exclusive boundary of Modernist Art. Modernism has been established on the basis of the opposites such as eyes and body, the spiritual and the material, art and non-art, avant-garde and kitsch, original and reproduction, maleness and femaleness, nature and technology, and then, arming with historical necessity, destined as unreversible law, universal moral, and fundamental essence through American theorists such like Alfred H. Barr,Jr., Clement Greenberg, and Michael Fried. It became a very meaningful turning point that in the sixties, the myth of Modernism was criticized and lost its validity, and reappreciated just like a historical happening by the stream of post-Modernism. But it leaves room for overlooking the discordance between American Modernist theories and historical modernists that we consider as though the myth of Modernism was destroyed just by the criticism and reflection of ex-post-facto. So this study focused on the fact that in the realm of Modernism there have been multiple discourses, which could not be unified as only one narrative, and also a lot of contradictions and ruptures threatening the purity of Modernism. This thesis selected two historical facts among them. One is that 'robot avant-garde', which overwhelmed modern art in the early 20th century, was excluded as marginal stream in the American Modernist theories. Robot avant-garde ardently worshiped mechanical man as a new subject and object of modern art, and as a 'New Man' of the new world of future. Therefore mechanical man was a symbol in which all the ideals of historical avant-gardes and Modernists were condensed. But the mechanical man, by taking both aspects of the hierarchical opposites premised in Modernist discourse, made the distinction between the pure and the impure invalid, and by revealing the points which that opposites were implicated with political, social, and cultural contexts, brought about the doubt of the purity and autonomy of Modernism itself. This doubleness of mechanical man made his existence invisible or marginal in the American Modernist theories. The other is a specific group which has been called as the Bauhaus. The experimentations of the Bauhaus were inclined to so-called 'unified art', and therefore expanded boundary of art included even daily life. To theorists who intended to give Modernism a rigid boundary, therefore, the existence of the Bauhaus was very troublesome. This troublesomeness was well expressed in the monumental lineage of modern art created by A. H. Barr, Jr.. The Bauhaus, which existed as a only cut-off in Barr's table, is a Modernist art and at the same time a non-Modernist art. It is boundary or joint itself between the visible lineage of art and non-artistic contexts existing as an empty blank in that table. For the project of the Bauhaus is the extension of art, with violating the boundary of art, and based on the autonomy of vision, with revealing mechanical body which that vision results from. Therefore, by re-locating robot avant-garde and the Bauhaus into the limited Modernist theories, this thesis intended to relativatize American Modernist discourse and to uncover the fact that Modernism itself was, having countless seams, heterogeneous and non-uniform tendency. The mechanical man of the Bauhaus was constructed through contemporary socio-political contexts and mass culture reversing the purity of Modernism, and the pure vision, accentuated in the field of Modernism, entered upon the stage with and as a part of mechanical body. Consequently, paradoxical aspects such like the mechanical man of the Bauhaus is not the others of Modernist art but one of various centers which made Modernism meaningful, and propose the possibility of multiple Modernism, overcoming singular Modernism which American Modernist theories and the criticism of post-Modernism to Modernism have approved as a premise.

Distribution of the Fairy Pitta (Pitta nympha) in the South Korea: A focus on protected areas

Gil-Pyo Hong(홍길표),Jae-Hoon Kim(김재훈),Seul-Gi Seo(서슬기),So-Yeon Cho(조소연),Bo-Yeon Hwang(황보연),Jong-Hyun Park(박종현),Seung-Yeon Lee(이승연),Ga-Young Kim(김가영),Wan-Hee Nam(남완희),Ha-Cheol Sung(성하철) 한국조류학회 2021 한국조류학회지 Vol.28 No.2

국내 팔색조(Pitta nympha) 분포 현황을 알아보고자 전국 국립공원 등 보호지역 중심으로 총 19개 지역을 조사하였고, 전문가 네트워크를 통해 자료를 보강하였다. 전국 19개 지역을 조사한 결과 북한산, 지리산 등 내륙지역 7개 국립공원에서는 팔색조가 확인되지 않았고, 경남 거제, 남해, 해남, 장흥 고흥 등 남해안과 인접한 12개 지역에서 팔색조 서식이 확인되었다. 또한 전문가 네트워크를 통해 수집한 팔색조 출현 위치 정보 82건을 분석한 결과, 전국 8개 광역지자체 모두 팔색조 서식이 확인되었고, 내륙지역(26.8%)보다 해안지역(73.2%)에서 더 많이 출현하였다. 연구 결과를 종합해보면, 우리나라의 팔색조는 주로 제주도와 남해안 지역을 중심으로 많이 분포하며, 그 보다 적은 개체수가 내륙 지역에 넓게 분포하고 있는 것으로 나타났다. The present study investigated the current distribution of Fairy Pitta (Pitta nympha) in South Korea. A total of 19 study areas, including protected areas such as national and provincial parks in South Korea were surveyed, and additional data obtained from an expert network. According to the results, Fairy Pitta was absent in seven inland national parks, including Mt. Bukhan and Chiri parks. Fairy Pitta inhabited 12 areas near the southern coast of South Korea, including Geoje, Namhae, Haenam, Jangheung, Goheung, and Gyeongnam Province. The analysis results of 82 datasets obtained from experts with regard to the locations where Fairy Pitta have been spotted showed that the bird inhabits all the eight metropolitan regions in South Korea, and is more frequently spotted in coastal areas (73.2%) than in inland areas (26.8%). The findings demonstrate that Fairy Pitta is mainly distributed in Jeju Island and the southern coast of South Korea, and is distributed in inland areas at relatively lower numbers.

습지생태공원의 갈대확장 조절 기술 개발

성기준 ( Ki June Sung ),이용민 ( Yong Min Yee ),정용현 ( Yong Hyun Chung ),박소영 ( So Young Park ) 한국환경보건기술학회(구 한국환경복원녹화기술학회) 2010 한국환경복원기술학회지 Vol.13 No.5

It is very difficult to control the expansion of reed at a wetland ecological park due to their aggressive reproduction capability. Therefore, proper topography and wetland hydrology should be provided before the construction of a wetland ecological park. Appropriate measures should be taken at the initial stages to prevent reed expansion. Field experiments were conducted at the newly constructed wetland ecological park for 15 months to develop methods to control the reproduction and expansion of Phragmites communis. A complete shading method had the best effect on the prevention of reed reoccurrence among other methods. Complete removal of the rhizomes controlled the reed growth to a greater extent than cutting the aboveground shoots. Water-level manipulation was also better than a half-shading method for the prevention of reed reproduction. These results indicated that soil properties and edge effects can influence the reed growth. Cutting only the aboveground shoots facilitates the growth of reed. These results suggest that reed control should be implemented repeatedly and an alteration in the wetland topography and hydrology may be more effective in the long term. Combination of treatments such as water-level management with shading should be considered to control the growth of reed. Adoptive management for created or restored ecosystem might be considered for accomplishment of its original purpose.

Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

2.9% 과산화수소를 함유한 부착형 미백제의 임상적 효능과 안정성에 관한 연구

박은숙,성소래,홍성태,김지은,이소영,황수연,이신재,진보형,손호현,조병훈 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.4

2.9%의 과산화수소를 함유한 시험용 부착형 미백제(Medison dental whitening strip, Samsung medical Co.,Anyang,Korea)를 실험군 피험자 23명, 시험용 미백제에서 과산화수소를 제외한 동일한 제재를 대조약으로 대조군 피험자 24명에게 매일 1시간 30분씩, 2주간 적용하여 임상적 효능과 안전성을 평가하였다. 미백 효과는 Shade Vision, Chroma Mete 및 Vitapan classical shade guide를 이용하여 색변화를 측정하여 다음과 같은 결론을 얻었다. 1. 실험군에서의 전체 치아의 색변화량(ΔE^(*))은 세 가지 측정법에서 모두 대조군에 비해 통계적으로 유의한 차이를 보였고 (p < 0.05). 쉽게 인지할 수 있는 정도였다. 2. 색변화는 색좌표 상에서 명도의 증가와 녹색조 및 청색조로의 변화에 의해 얻어졌다. 3. 치아별로는 어두운 견치의 색변화량이 가장 컸고, 측절치, 중절치 순으로 감소하였다. 4. 47명의 피험자 중 5명이 경도의 과민증을 경험하였으나 곧 완화되었다, 치은염증지수와 전기치수검사 결과 시험 전후에 차이가 없어 안전성을 확인하였다. This study evaluated the effectiveness and safety of an experimental bleaching strip (Medison dental whitening strip, Samsung medical Co., Anyang, Korea) containing 2.9% hydrogen peroxide. Twenty-three volunteers used the bleaching strips for one and a half hour daily for 2 weeks. As control group, the same strips in which hydrogen peroxide was not included were used by 24 volunteers with the same protocol. The shade chang (ΔE^(*), color differnce) of twelve anterior teeth was measured using Shade Vision (X-Rite Inc., S. W. Grandville, MI, USA), Chroma Meter (Minota Co., Ltd. Osaka, Japan) and Vitapan classical shade guide (Vita Zahnfabrik, Germany). The shade change of overall teeth in the experimental group was significantly greater than that in the control group (p < 0.05) and was easily perceivable. The change resulted from the increase of lightness (CIE L^(*) value) and the decrease of redness (CIE a^(*) value) and yellowness (CIE b^(*) value). The shade change of individual tooth was greatest in canine, can smallest in central incisor. The safety of the bleaching strip was also confirmed. 〔J Kor Acad Cons Dent 31(4):269-281, 2006〕

간 이식 환자에서 발생한 파종성 Scedosporium apiospermum 감염 1예

박현구,최성호,강호석,송주형,정지훈,소민욱,최상호,김양수,우준희 대한감염학회 2006 감염과 화학요법 Vol.38 No.5

저자들은 다발성 뇌 농양을 동반한 치명적인 파종성 S. apiospermum 감염이 발생한 간 이식 환자를 경험하였다. 본 증례의 환자는 voriconazole로 치료하는 호전경과를 보이다가 진균성 뇌 동맥류 파열로 사망하였다. S. apio-spermum은 비교적 드문 진균으로 병리학적으로 Aspergillus spp.와 매우 유사하고 amphotericin B에 내성을 나타내므로 진단과 치료가 적절하게 이루어지지 못할 가능성이 많고 면역 기능 저하 환자에서 혈행성 전파를 통한 파종성 감염이 용이하게 발생하므로 높은 사망률을 나타낸다. 이식 환자를 비롯한 면역 기능 저하 환자에서 진균감염이 의심될 때 S. apiospermum과 같은 드문 진균에 의한 감염증도 감별진단에 포함해야 하겠다. Sedosporium apiospermum is a saprophytic fungus commonly found in soil and polluted water. This organism is known as a cause of mycetoma, which may occur in immunocompetent hosts following trauma. However, in immunocompromised patients, S. apiospermum can also cause life-threatening invasive disease, including central nervous system infection or disseminated infection. We report a fatal case of disseminated S. apiospermum infection in a 46-year-old woman after liver transplantation. Eight days postoperatively, she developed pneumonia, followed by altered mentality in the 15 days. A head CT demonstrated multiple brain abscesses. Sputum and stereotactic-aspirated brain abscess culture yielded S. apiospermum. Despite treatment with voriconazole, the patient died of intracranial hemorrhage.