우치 법랑질의 레이저 및 산부식처리 후 결합강도에 관한 실험적 연구

차경,김재곤,백병주,이승익 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

The purpose of this study was to determine the optimal energy level to the enamel surface of bovine teeth when they are irradiated by pulsed Nd:YAG laser and was to compare acid etching with laser etching. For the study, the labial enamel surfaces of extracted intact bovine teeth were irradiated with normal pulsed Nd:YAG laser by six different energy of 30, 60, 80, 100, 120 and 160mJ. The author observed to enamel surface with a scanning electron microscope and measured the shear bond strength between etched enamel surface and composite resin by universal testing machine with a crosshead speed of 1mm/min. and investigated difference acid and laser etching group. The obtained results were as followng : 1. The shear bond strength were 24.26 MPa in Group Ⅰ, 5.50 MPa in Group Ⅱ, 5.77 MPa in Group Ⅲ, 5.90 MPa in Group Ⅳ, 6.01 MPa in Group Ⅴ, 6.34 MPa in Group Ⅵ, 6.24 MPa in Group Ⅶ. Acid etching Group(GroupⅠ) was showed higher bond strength than other laser etching groups(p<0.05). 2. In laser etching groups, the shear bond strength was highest in 120mJ energy level(Group Ⅵ). 3. Group Ⅱ, Ⅲ and Ⅳ, below 80mJ energy level were observed only microcrack and small projection in 80mJ energy level(Group Ⅳ) and group Ⅴ, Ⅵ and Ⅶ were observed small pore and crater in 5∼9㎛ diameter, especially in 120mJ energy level(Group Ⅵ).

Clinical characteristics of in situ pulmonary artery thrombosis in Korea

( Seung Ick Cha ),( Keum Ju Choi ),( Seung Soo Yoo ),( Jaehee Lee ),( Shin Yup Lee ),( Chang Ho Kim ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.0

Objectives: Little is known regarding the clinical features and course of in situ pulmonary artery thrombosis (PAT). The aim of the present study was to investigate the clinical characteristics of PAT. Methods: Patients with PAT were retrospectively identified from a tertiary referral center in South Korea. A control group consisted of patients with pulmonary embolism (PE) in whom the right or left pulmonary artery was the largest PE-involved site. We compared various clinical parameters between the two groups. Results: Of 23 PAT patients, the most common underlying condition was tuberculosis (TB)-destroyed lung (11 [47.8%]), followed by pulmonary artery stump after lobectomy or pneumonectomy (7 [30.4%]). In all patients except one, PAT was located in the right or left pulmonary artery. Computed tomography scans demonstrated that clots were completely or partially resolved less frequently in the PAT group than in the control group (4 [25%] versus 62 [90%], p<0.001). In the PAT group, three of seven patients (43%) who had undergone anticoagulation therapy exhibited improvement, and one of nine (11%) who had not received anticoagulation therapy experienced improvement; however, the difference was not significant. Conclusions: TB-destroyed lung was the most common underlying condition in Korean PAT patients, followed by pulmonary artery stump after lung resection. The clots in patients with PAT were mostly located in the right or left pulmonary artery, and clot resolution was less frequent in the PAT group compared to the PE group.

Ultrasonic sprayed multi-wall carbon nanotube for counter electrode application in dye-sensitized solar cells

Seung Jun Min,Hyun Ju Kim,Kyung Ju Lee,Seung Il Cha,Byung Moo Moon 한국진공학회 2009 한국진공학회 학술발표회초록집 Vol.37 No.-

Clinical characteristics of pulmonary embolism with concomitant pneumonia

Cha, Seung-Ick,Choi, Keum-Ju,Shin, Kyung-Min,Lim, Jae-Kwang,Yoo, Seung-Soo,Lee, Jaehee,Lee, Shin-Yup,Kim, Chang-Ho,Park, Jae-Yong YEAR Wolters Kluwer Health, Inc. All rights reserv 2016 Blood coagulation & fibrinolysis Vol.27 No.3

<P>Although pneumonia is associated with an increased risk of venous thromboembolism, patients with pulmonary embolism and concomitant pneumonia are uncommon. The aim of the present study was to investigate the clinical features of pulmonary embolism with coexisting pneumonia. We retrospectively compared clinical, radiologic and laboratory parameters between patients with pulmonary embolism and concomitant pneumonia (pneumonia group) and those with unprovoked pulmonary embolism (unprovoked group), and then between the pneumonia group and those with pulmonary infarction (infarction group). Of 794 patients with pulmonary embolism, 36 (5%) had coexisting pneumonia and six (1%) had no provoking factor other than pneumonia. Stroke was significantly more common in the pneumonia group, than either the unprovoked group or the infarction group. In the pneumonia group, fever was significantly more common and serum C-reactive protein levels were significantly higher. By contrast, central pulmonary embolism and right ventricular dilation on computed tomography were significantly less frequent in the pneumonia group. In addition, an adverse outcome due to pulmonary embolism was less common in the pneumonia group than in either of the other two groups. The coexistence of pulmonary embolism and pneumonia is rarely encountered in clinical practice, especially without the presence of other factors that could provoke venous thromboembolism and is commonly associated with stroke. It is characterized by lower incidences of central pulmonary embolism and right ventricular dilation and by a lower rate of adverse outcomes due to pulmonary embolism itself.</P>

Comparison of immunogenecities of three beta-nodavirus proteins, capsid protein, non-structural protein Bi and B2 in olive flounder

Seung Ju Cha,Jeong Wan Do,Myoung Seok Ko,Jin Woo Kim,Jeong Woo Park 한국어병학회 2009 한국어병학회지 Vol.22 No.3

브로콜리(Brassica oleracea var. italica)의 온도 스트레스 평가를 위한 다중 센서 모니터링

차승주 ( Seung-ju Cha ),박현준 ( Hyun Jun Park ),이주경 ( Joo-kyung Lee ),권선주 ( Seon-ju Kwon ),지효경 ( Hyo-kyung Jee ),백현 ( Hyun Baek ),김한나 ( Han-na Kim ),박진희 ( Jin Hee Park ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4

기상이변으로 인한 식물 스트레스를 평가하기 위해 토양과 식물에 적용할 수 있는 여러 센서가 개발되었다. 따라서 본 연구의 목적은 식물의 온도 스트레스를 평가하기 위해 다양한 센서를 사용하여 기후 및 토양 상태와 식물 반응을 모니터링하는 것이다. 식물 반응을 평가하기 위한 식물 센서로 식물 줄기의 전기전도도, sap flow rate를 모니터링하였다. 식물 줄기의 전기전도도는 물과 이온 수송을 포함한 식물의 생리적 활성을 반영한다. 완전히 자란 Brassica oleracea var. italica를 온도 20/15 ℃ (낮/밤), 16시간 광주기로 하여 대조구로 설정하였으며 저온 15/10 ℃ 및 고온 35/30 ℃에 노출시키면서 토양 및 식물 상태를 모니터링하였다. 식물 줄기의 전기전도도, sap flow rate는 낮에는 증가하고 밤에는 감소하였다. 저온 스트레스 하에서 Brassica oleracea var. italica 줄기의 전기전도도는 대조구보다 낮았고, 고온 스트레스 하에서는 대조구보다 높아 물과 이온 수송이 온도에 의해 영향을 받았음을 나타낸다. 그러나 엽록소 a와 b 함량은 저온 스트레스를 받은 잎에서 증가했고 고온 스트레스를 받은 잎에서는 대조구와 차이가 없었다. 잎의 프롤린 함량은 저온 스트레스에서는 대조구와 차이가 없는 반면, 고온 스트레스에서는 증가했다. 프롤린 합성은 환경 스트레스 하에서 식물의 방어 메커니즘으로 작용한다. 따라서 Brassica oleracea var. Italica는 저온보다 고온 스트레스에 더 민감한 것으로 판단된다. Several sensors have been developed for soil and plants to assess plant stress due to climate change. Therefore, the objective of the study is to nondestructively evaluate temperature stress on plant by monitoring climatic and soil conditions and plant responses using various sensors. Plant responses were monitored by electrical conductivity in plant stem and sap flow rate. Electrical conductivity in plant stem reflects the physiological activity of plants including water and ion transport. Fully grown Brassica oleracea var. italica was exposed to 20/15 ℃ (day/night) with 16 h photoperiods as a control, low temperature 15/10 ℃, and high temperature 35/30 ℃ while climatic, soil, and plant conditions were monitored. Electrical conductivity in plant stem and sap flow rate increased during the day and decreased at night. Under low temperature stress, electrical conductivity in plant stem of Brassica oleracea var. italica was lower than control while under high temperature stress, it was higher than control indicating that water and ion transport was affected. However, chlorophyll a and b increased in leaves subjected to low temperature stress and there was no significant difference between high temperature stressed leaves and control. Free proline contents in the leaves did not increase under low temperature stress, but increased under high temperature stress. Proline synthesis in plant is a defense mechanism under environmental stress. Therefore, Brassica oleracea var. Italica appears to be more susceptible to high temperature stress than low temperature.

A new approach of digital PCR system for non-invasive prenatal screening of trisomy 21

Lee, Seung Yong,Kim, Seung Jun,Han, Sung-Hee,Park, Joon Soo,Choi, Hyo Jung,Ahn, Jeong Jin,Oh, Moon-Ju,Shim, Sung Han,Cha, Dong Hyun,Hwang, Seung Yong Elsevier 2018 Clinica chimica acta Vol.476 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Non-invasive prenatal screening (NIPS) of trisomy 21 (T21) using digital PCR (dPCR) with several advantages will be very effective. Here, we developed a dPCR system for T21 screening which allows high sensitivity and real-time diagnosis and thus overcome sequence based analysis.</P> <P><B>Methods</B></P> <P>Cut-off value was established using DNA extracted from all 157 T21 negative samples including 47 pregnant woman samples and 3 T21 positive pregnant woman samples extracted from 4 different sample types. To increase the portion of the cell-free fetal DNA (cffDNA) in maternal cell-free DNA (cfDNA), a size selection method was devised. We evaluated the clinical reliability of NIPS using dPCR through analysis of 877 pregnant woman samples.</P> <P><B>Results</B></P> <P>We could demonstrate the possibility of NIPS using dPCR performed by applying cut-off value and size selection method. The overall accuracy was derived at 99.66% using 877 pregnant woman plasma samples.</P> <P><B>Conclusion</B></P> <P>Our results showed that dPCR can meet the requirements for NIPS for T21. It is relatively inexpensive, easy to use in a screening method and compatible with ethical concerns regarding access to nucleotide sequence information. This study may be a basic data for the development of population-wide screening for T21 in pregnant women.</P> <P><B>Highlight</B></P> <P> <UL> <LI> NIPS for trisomy 21 using dPCR is easy to apply and results are obtained quickly </LI> <LI> We have developed the technology using digital PCR to screening for trisomy 21 </LI> <LI> We evaluated the accuracy of development technology using clinical samples </LI> <LI> This technique can be extended to the detection of other aneuploidies using cfDNA </LI> </UL> </P>

처리방법에 따른 사과 중 잔류농약 제거효과

차승주 ( Seung-ju Cha ),송영진 ( Young-jin Song ),김보미나라 ( Bo-mi-na-ra Kim ),권세희 ( Se-hee Kwan ),조한울 ( Han-ul Joh ),김민주 ( Min-ju Kim ),조마리아 ( Ma-ri-a Jo ),유영신 ( Young-sin Yu ),황신구 ( Sin-gu Hwang ),윤광일 ( Kwan 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-

농약은 농작물이 잡초나 해충, 세균으로부터 받는 피해를 예방하고 농작물의 생산성 증진 등으로 농업에서 중요한 역할을 하는 농자재이다. 친환경 인증 농산물 외 일반 농산물은 허용기준 이내라도 농약이 잔류할 가능성이 있어 소비자들은 농산물 섭취 시 잔류농약 노출에 불안감을 갖고 있다. 이러한 불안감을 줄이기 위하여 과일에 잔류하는 농약을 효과적으로 제거하는 방법을 규명하기 위한 연구를 진행하였다. 본 실험은 친환경 사과를 처리구당 3개씩 3반복으로 나뉘어 살균제(Carbendazim, Trifloxystrobin), 살충제(Acetamiprid, Diflubenzuron)를 살포한 후, 7일 후에 무처리, 껍질 제거(제거), 물 세척(세척), 물 침지 후 세척(침지), 식초물 침지 후 세척(식초), 소다물 침지 후 세척(소다)의 방법으로 처리한 후 잔류농약을 분석하였다. 침지시간은 5분, 세척시간은 20초로 하였다. 잔류농약 분석을 위한 전처리는 균질화 된 시료 10 g을 50 mL 원심분리관에 칭량하여 Acetonitrile을 10 mL 주가한 후 1분간 진탕하고, 4 g MgSO₄, 1g NaCl, 1g NaCitrate, 0.5 g Disodium citrate sesquihydrate를 첨가한 후 다시 1분간 균질기로 진탕한 다음 3,000 rpm/min으로 5분간 원심분리를 진행하였다. 상등액 1ml를 Dispersive SPE(25mg PSA, 150 mg MgSO4) tube에 넣고 1분간 진탕하고, 10,000 rpm에서 1분간 원심분리하여 상등액을 0.2 μm 시린지 필터로 여과하여 LC/MS/MS, GC/MS/MS 분석시액으로 사용하였다. 분석방법은 「농산물 잔류농약 다성분 분석방법」에 따라 4가지 농약성분을 분석하였다. 분석 결과 Carbendazim(살균제)은 무처리 대비 제거 88%>소다 72%>침지 64%>식초 61%>세척 55% 순으로 제거되는 결과를 나타냈다. Trifloxystrobin(살균제)은 제거 87%>소다 79%>식초 75%>세척 63%>침지 60% 순으로 제거되었다. Acetamiprid(살충제)는 무처리 대비 제거 91%>식초 63%>소다 59%>침지 50%>세척 47% 순으로 제거되는 결과를 나타냈다. Diflubenzuron(살충제)은 소다 87% >제거 80%>침지 78%>세척 76%>식초 67% 순으로 제거되었다. 4가지 성분 모두 껍질을 제거한 방법에서 잔류농약이 효과적으로 제거되었다. 세척에 따른 결과는 소다침지 후 세척이 Acetamiprid 외 3가지 농약에서 제거효과가 가장 좋았다. 농약성분 별로 처리방법에 따른 제거효과가 다른 결과에 대하여서는 추가적인 연구가 필요할 것으로 사료되었다.

수신 8채널 송신 1채널 79 GHz Sparse Array FMCW 레이다 시스템

차승호(Seung-Ho Cha),박재현(Jae-Hyun Park),김승연(Seung-Yeon Kim),한예주(Ye-Ju Han),이재원(Jae-Won Lee),김병성(Byung-sung Kim) 한국전자파학회 2023 한국전자파학회논문지 Vol.34 No.10

식물 센서를 활용한 다양한 환경 변화에 따른 Capsicum annuum의 반응 평가

차승주(Seung-Ju Cha),박현준(Hyun Jun Park),이주경(Joo-Kyung Lee),박진희(Jin Hee Park) 한국토양비료학회 2020 한국토양비료학회 학술발표회 초록집 Vol.2020 No.10