Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

防風類의 比較檢討

김미경,김미정,김은영,류건영,이은미,장원희,황선희 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

의약분업 전후의 의약정보서비스의 질의응답과 질의자의 만족도 분석

신정인,김미애,허경희,김미정,신현택,오정미 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Drug Information Centers(DICs) are responsible for providing updated and relevant drug information on the efficacy, safety and quality of drugs to health-care practitioners and finally to patients. After the establishment of new prescription law(Bunup)' on August 2000, the future direction for DICs is strongly needed to provide specified, appropriate and rapid information to health-card practitioners and patients. This project was undertaken to provide a future direction of DICs via comparing differences of Q&A before and after Bunup, based on the analysis of Q&A worksheets that were conducted from August 1999 to July 2001 at Drug Information Research Institute(DIRI) of Sookmyung Women's University. In addition, feedback sheets were collected from the users of DIRI from April 2001 to August 2001, to evaluate the satisfaction scores by '5-point Likert scale' on the response. The number of inquiries responded by DIRI was increase from 201 to 574 and the most frequently asked inquirers were pharmacists, specifically community pharmacists. The mean time to respond before and after Bunup was 5.35 and 4.68 hours, respectively. The method of inquiry utilized the most was electronic mailing system followed by telephone both before and after Bunup(66.2% vs. 65.7% via e-mail, from 32.8% vs. 32.4% via telephone). The most frequently asked category of question was on the 'clinical drug' category both before and after Bunup (79% vs. 73.6%). The tertiary literature was the most frequently used reference to answer the inquiries. Mean feedback result via questionnaires was good. That included accuracy, quality of information, time to respond, attitude of provider, accessibility, etc. The most increased inquirer after Bunup was general person, compared with before. Therefore DICs should provide separate responds according to inquirers, develop the systemic program to improve the response for each request, have systemic Q&A worksheet, and develop the educational program for drug information providers. DICs should continuously provide more appropriate and rapid information to health-care practitioners and patients.

시각적 피드백을 병행한 어깨복합운동프로그램이 어깨비대칭 청년들의 어깨 근력과 정렬에 미치는 영향

강순희, 강은지, 목미경, 박지연, 손혜림, 연우정 한국교통대학교 2016 한국교통대학교 논문집 Vol.51 No.-

The purpose of this study was to identify whether shoulder complex exercise program with visual feedback could improve the shoulder muscular strength and postural alignment in adolescents with shoulder asymmetry. Twenty eight subjects were recruited from the K university and divided into three groups: experimental 1, 2group(Each n=10) and control group(n=8). For 4 weeks, the experimental group 1 performed shoulder muscle complex exercise program and the experimental group 2 performed shoulder complex exercise program with visual feedback. The control group did not perform exercises. The shoulder muscle strength and difference between the height of left and right shoulders were assessed before, after training using the hand-held dynamometer and Global Posture System. Subordination variables, general characteristics analysis, χ² test and Kruskal Wallis H test were conducted to evaluate differences between groups and training before, after changes were checked Wilcoxon signed rank test with using the PASW 18.0 statistical program. The results of the study were as follows. 1) Both the experimental 1, 2group showed improvements in muscle strength of several muscles following training (p<.01). The control group showed significant reduction in the left posterior deltoid (p<.05), and did not show significant changes in examining other muscles (p>.05). And there were differences among three groups in shoulder muscle strength (p<.001). Muscle strength changes in several muscles were greater in the experimental 1, 2group compared with the control group (p<.001). Muscle strength changes in the several muscles were greater in the experimental group 2 compared with the experimental group 1 (p<.001) 2) Both the experimental 1, 2group showed significant decreases in the difference between the left and right shoulder heights following training (p<.01). The control group did not show change (p>.05). Changes in the difference between the left and right shoulder heights were greater in the experimental 1, 2group compared with the control group (p<.001).

물 없이 사용하는 알코올 젤의 손소독 효과에 대한 연구

정재심,김덕희,김미나,최명애 대한기초간호자연과학회 2002 Journal of korean biological nursing science Vol.4 No.2

The purpose of this study was to compare the hand disinfection effect of waterless alcohol gel hand washing agent with that of soap and water, 4% chlorhexidine gluconate, and 10% povidone-iodine. Hands of fourty subjects were artificially contaminated with Acinetobacter baumannii 5㎖ and randomly distributed to each hand washing methods. Samples were collected from gloved hand by glove juice sampling procedure. Mean log reduction after hand washing were compared with baseline values. Number of microorganisms were converted to log and tested by ANOVA in SPSSWIN 100. Mean log reduction of soap and water, alcohol gel, 4% chlorhexidine gluconate, 10% povidone-iodine were 2.76±0.62, 2.97±0.56, 4.66±1.70, 4.60±0.91, respectively. The bactericidal effect of alcohol gel was similar to that of soap and water, but the effect was much less than chlorhexidine gluconate and povidone-iodine(p<0.001). In terms of microorganism reduction, the efficacy of waterless alcohol gel was almost the same as soap and water hand washing. Further evaluation of the bactericidal effect of waterless alcohol gel is needed because waterless alcohol gel is simple, convenient, and non-irritating hand washing agent and also very effective in busy hospital environment.

Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression

Lee, Mi Nam,Hwang, Hee-Su,Oh, Sin-Hye,Roshanzadeh, Amir,Kim, Jung-Woo,Song, Ju Han,Kim, Eung-Sam,Koh, Jeong-Tae Nature Publishing Group UK 2018 Experimental and molecular medicine Vol.50 No.11

<▼1><P>Supplementation of mesenchymal stem cells (MSCs) at sites of bone resorption is required for bone homeostasis because of the non-proliferation and short lifespan properties of the osteoblasts. Calcium ions (Ca<SUP>2+</SUP>) are released from the bone surfaces during osteoclast-mediated bone resorption. However, how elevated extracellular Ca<SUP>2+</SUP> concentrations would alter MSCs behavior in the proximal sites of bone resorption is largely unknown. In this study, we investigated the effect of extracellular Ca<SUP>2+</SUP> on MSCs phenotype depending on Ca<SUP>2+</SUP> concentrations. We found that the elevated extracellular Ca<SUP>2+</SUP> promoted cell proliferation and matrix mineralization of MSCs. In addition, MSCs induced the expression and secretion of osteopontin (OPN), which enhanced MSCs migration under the elevated extracellular Ca<SUP>2+</SUP> conditions. We developed in vitro osteoclast-mediated bone resorption conditions using mouse calvaria bone slices and demonstrated Ca<SUP>2+</SUP> is released from bone resorption surfaces. We also showed that the MSCs phenotype, including cell proliferation and migration, changed when the cells were treated with a bone resorption-conditioned medium. These findings suggest that the dynamic changes in Ca<SUP>2+</SUP> concentrations in the microenvironments of bone remodeling surfaces modulate MSCs phenotype and thereby contribute to bone regeneration.</P></▼1><▼2><P><B>Bone formation: released calcium could stimulate stem cells</B></P><P>Studies with mouse cells and bone samples indicate how calcium ions released during the normal turnover of bone promote the supplementation of stem cells needed to make new bone during routine “remodeling” and regeneration after injury. Our bones are degraded and remodeled continuously, requiring the controlled supplement of stem cells that create bone-forming cells called osteoblasts. Jeong-Tae Koh and colleagues at Chonnam National University, Gwangju, South Korea, exposed the relevant stem cells to increased concentrations of external calcium ions. This promoted cell proliferation and migration that allow the cells to support new bone formation. The research suggests this effect on stem cells may be achieved by calcium ions released directly from bone as it is naturally degraded. The findings may help researchers develop new ways to encourage bone regeneration after injury and surgery.</P></▼2>

프로토콜 분석을 통한 창의적 디자인 전략으로서의 은유와 유추

최한희(Choi, Han_Hee),주서령(Ju, Seo Ryeung),김미정(Kim, Mi Jeong) 한국실내디자인학회 2013 한국실내디자인학회논문집 Vol.22 No.1

The aim of this research is to explore the potential of analogy and metaphor as design strategies for supporting the creative design thinking. For the empirical research on analogical and metaphoric process in designing, we conducted design experiments that consist of three different conditions of given items as stimulation for design inspiration: baseline, surrealist paintings, and housing design collections. The results were analyzed using a protocol analysis in order to obtain more systmatic interpretation of the design processes and strategies. As a result, it was noted that students are more apt to read visual information rather than semantic information in the given items. Instead of the representation of their senses or feelings from the paintings, they visualized the analogical images of the paintings for the design representation. However, analogical and metaphoric thinking derived from the given items seem change a designer’s perspective, thus bring a novel interpretation on design problems, and eventually more creative and meaningful design ideas. An extended research using one-semester training and observation of the design studio process is introduced as a follow-up study for this paper. This research will investigate the long-term effect of the analogy and metaphor on the design thinking.

화생성 유방암의 영상 소견 : 증례 보고

정미희,정경순 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Metaplastic carcinomas of the breast comprise a heterogeneous group of neoplasms that are regarded as a ductal carcinoma that undergos metaplasia into a nonglandular growth pattern. Metaplastic carcinoma is uncommon, accounting for fewer than 5% of breast carcinomas. It has a poorer prognosis than most other breast malignancies. We report the mammographic and sonographic findings in a 52-year-old woman of pathologically proven metaplastic carcinoma.