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고정삼,강순선,오현도 濟州大學校 亞熱帶農業硏究所 1995 亞熱帶農業硏究 Vol.12 No.-
제주산 조생온주밀감과 보통온주의 크기별 과즙제조에 대한 가공적성을 검토하였다. 감귤이 커질수록 껍질이 두꺼워지고 과형지수가 커졌으며, pH가 약간 높아졌다. 중간크기의 감귤이 가용성고형물과 과즙율이 높았으며, 소형과 또는 대형과는 가공적성이 떨어졌다. 따라서 가공용 감귤의 경우도 생과용과 마찬가지로 내용성분에 의한 등급화로 가공공장의 경쟁력을 향상시킬 필요가 있을 것으로 여겨진다. Juice processing characteristics of Citrus unshiu produced in Cheju were investigated. Fruit weight, peel thickness, fruit index and pH of early and medium type of Citrus unshiu were highered linearly with increasing fruit size. Soluble solids and juice ratio were high in middle size of fruit, and juice processing characteristics was low in small and large size of citrus fruits. Grading of citrus fruits would be also needed for juice processing indusry.
Cellular Factors Binding on the Core Origin of Simian Virus 40(SV40)DNA Replication
Kang, Hyen Sam,Kim, Yeon Soo 한국유전학회 1988 Genes & Genomics Vol.10 No.4
The 110 bp HindIII-NcoI digested fragment of SV40 DNA contains the 65-bp core origin of replication and a part of SV40 large T antigen binding site I including the 5′-GAGGC-3′ pentanucleotide repeats. We have used gel retention assays to define monkey cell factors that interact with the DNA fragment carrying this sequences. Two main DNA-protein complexes formed with extracts were observed. DNase I foot-printing with these DNA-protein complexes showed that the perfect palindrome of large T antigen binding site II and 5′-GAGGC-3′ region interact with some cellular proteins. Also the DNase I footprinting pattern of mutant templates showed that the early palindrome domain was involved in the binding of cellular factor and the replication activity of the origin as a sequence-specific manner.
Kang, Hyen Sam,Kim, Yeon Soo,Koo, Yong Eui 한국유전학회 1988 Genes & Genomics Vol.10 No.4
To define the role of the early palindrome domain within the core origin of simian virus 40 (SV40) DNA replication, 800 bp DNA fragment containing SV40 origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13 SV-2, by oligonucleotide-directed site-specific mutagenesis. Bacteriophage M13 DNAs carrying the wild-type or base substituted SV40 origin of replication were used for replication assay. In vivo and in vitro assay with african green monkey kidney cell lines showed that the replication of M13-SV40 recombinant DNAs was restricted like a pBR322-SV40 recombinant DNA (Lusky and Botchan, 1981). Furthermore, recombinant phage DNAs isolated from the transfected simian cells subsequently showed a reduced ability to retransform E. coli. Then, the origin fragments containing one or two base substitutions were subcloned into plasmid pAT153, a derivative of pBR322. In two mutants (pATSV-A, -B) the stems were destroyed by single base substitutions. In the other two mutants (pATSV-C, -D) the normal stems were restored by additional base initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease of DNA replication in monkey cells. These results suggest that the early palindrome domain does not function as a cruci-form structure, but may be involved in DNA replication initiation as a sequence-specific manner.
UVSC of Aspergillus nidulans is a Functional Homolog of RAD51 in Yeast
Kang, Hyen Sam,Seong, Kye Yong,Yoon, Jin Ho,Chae, Suhn Kee 생화학분자생물학회 1990 BMB Reports Vol.34 No.5
A defect in uvsC of Aspergillus nidulans caused high methyl methansulfonate (MMS)-sensitivity, hyporecombination, and a lack of UV induced mutation. The uvsC gene of Aspergillus nidulans shares a sequence similarity with the RAD51 gene of Saccharomyces cerevisiae. In this study, in vitro and in vivo tests were conducted in order to determine whether or not the UVSC protein had functional similarities to RAD51, the recombination enzyme in yeast. The purified recombinant UVSC protein, following expression in Escherichia coli, showed binding activity to single-stranded DNA (ssDNA), when both ATP and magnesium are present. In addition, ATPase activity was also demonstrated and its activity was stimulated in the presence of ssDNA. The UVSC protein that was expressed under the ADH promoter in S. cerevisiae suppressed in part the sensitivity to MMS of the rad51 null mutant. Similarly, when the uvsC cDNA was expressed from the nmt promoter, the MMS sensitivity of the rhp51 null mutant of Schizosaccharomyces pombe was partially complemented. These results indicate that the A. nidulans UVSC protein is a functional homologue of the RAD51 protein.