Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

인터넷상에 올려진 Radiology Teaching File에 관한 연구

임은정,김은경 大韓口腔顎顔面放射線學會 1996 Imaging Science in Dentistry Vol.26 No.2

There is increasing attention about radiology teaching files on the Internet in the field of diagnostic radiology. The purpose of this study was to aid in the creation of new radiology teaching file by analysing the present radiology teaching file sites on the Internet with many aspects and evaluating images on those sites, using Macintosh Ⅱci computer, 28.8kbps TelePort Fax/Modem, Netscape Navigator 2.0 software The results were as follow: 1. Analysis of radiology teaching file sites (1) Country distribution was the highest in USA(57.5%). (2) Average number of cases was 186 cases and radiology teaching file sites with search engine were 9 sites (22.5%). (3) At the method of case arrangement, anatomic area type and diagnosis type were found at the10 sites(25%)each, question & answer type was found at the 9 sites(22.5%). (4) Radiology teaching file sites with oro-maxillofacial disorder were 9 sites(22.5%). (5) At the image format, GIF format was found at the 14 sites(35%). and JPEG format found at the 14 sites(35%). (6) Created year was the highest in 1995(43.7%). (7) Continuing case upload was found at the 35 sites(87.5%). 2. Evaluation of images on the radiology teaching files (1) Average file size of GIF format (71 Kbyte) was greater than that of JPEG format(24 Kbyte). (P<0.001) (2) Image quality of GIF format was better than that of JPEG format. (p<0.001)

耐熱性 合成樹脂에 關한 硏究 : Aromatic Polyamide의 合成과 耐熱性에 關하여

林龍進,金祐埴,민경은 慶北大學校 1974 論文集 Vol.18 No.-

Thermally stable polymer which does not lose weight rapidly up to about 400℃ was prepared from p-aminobenzoic acid by means of melt polymerization. The polymer was identified completely para oriented aromatic polyamide by analytical data. And some information obtained from synthetic process and thermal and/or chemical stability of these polymers was discussed.

자질 유인 이론과 조동사 도치 현상

임은경 대전대학교 인문과학연구소 2001 人文科學論文集 Vol.31 No.-

The purpose of this paper is to analyze the auxiliary inversion phenomenon in English interrogative clauses within the framework of the attract-feature theory in the minimalist program. There are basically three types in distribution as the followings auxiliary inversion with overt wh-phrase movement, no auxiliary inversion with overt wh-phrase movement, and no auxiliary inversion without overt wh-phrase movement. To explain the distribution properties, I propose the following: The features of C which should be checked in the course of derivation are Q-affixal feature and wh-feature. Q-affixal feature is divided into two types, verbal affixal feature and non verbal affixal feature, following Kayne (1982). Verbal affixal feature induces the auxiliary inversion or do-support if it is strong. Its strength is determined by overt wh-movement. Whether a wh-phrase is attracted to [Spec, CP] or not is derived automatically in the course of derivation following the economy principle. I have shown my proposal is adequate in that it explains subject and object/adjunct asymmetry in wh-interogative clauses and the differences between matrix and embedded clauses, etc.

Chlorhexidine varnish가 법랑질의 탈회예방에 미치는 영향

임은경,최영철 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4

The intention of this study was to investigate the preventive effect of chlorhexidine varnish on enamel demineralization. The sample consisted of 57 first premolars scheduled to be extracted for orthodontic purposes. The control group (N=10) was left untreated and the experimental groups were worn with specially designed stainless steel orthodotic bands on premolar for plaque accumulation. The group(N=9) was worn band only, the group 2 (N=19) was applied with chlorhexidine varnish for one time, and the group 3(N=19)was applied with chlorhexidine varnish for 3 times once a week. After 4 weeks of experimental periods, every specimenwere examined by SEM and Vickers hardness test to evaluate and compare the degree of enamel decalcification. The results were as follows: 1. Although SEM revealed various degree of enamel demineralization in every experimental groups, the group 1 showed more severe demineralizations than the group 2and 3. 2. The mean Vickers Hardness Numbers measured in this study seemed to reveal that there was a statistically significant difference between the control goup and the group 1 (p<0.05), and also a significant defference between the group 1 and the group 2,3(p<0.05). And there was no significant difference between the group 2 and the group 3 (P>0.05). 3. The results of VHN did not deemed to show a statistically significant difference between maxillary premolar and mandibular premolar in both group 2 and group 3 (p>0.05).

PC 기반의 플랫폼간의 상호 연동을 위한 Intelligent Mirroring

임경은,황대준 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

Creating collaborative environment on heterogeneous PC-based multimedia platforms running on different operationg systems has been of great importance to allow natural interaction among remote users through interoperability. Intelligent Mirroring is incorporated into our Multimedia Distance Learning System for IBM PCs and Apple's Power Macintoshs running Windows (ver. 3.1, ver. 95) and System (ver. 7.1, ver. 7.5), respectively. Upon messages from platforms via Ethernet involved the distance learning situation, Intelligent Mirroring its functions based on system homogeniety: liasion of messages for homogeneous platforms, interpretation of events and conversion of data encoding formats for heterogeneous platforms involved in the interaction. In such a distance learning situation requiring high degree of interaction Intelligent Mirroring contributes not only to establishing function and content sharing at a fine grain level with low cost, but also to minimize traffics related to interpretation of events to be shared by platforms participating in the collaboration.

운동이 안면두개골의 형태에 미치는 영향

임은경,최영철 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.4

호흡기능 또는 근기능이 얼굴의 구조적 관계에 미치는 영향을 평가하고자 어려서부터 한가지 운동종목을 전공하여 온 체육대학 학생 137명을 운동의 유형에 따라, 지속적으로 심폐지구력이 많이 요구되는 운동군(1군), 심폐지구력이 많이 요구되기는 하나 1군보다는 적게 요구되는 운동군(2군), 그리고 순간적인 근력이 크게 요구되는 운동군(3군)으로 분류하고, 정모 및 측모 방사선사진을 계측학적으로 평가하여 다음과 같은 결론을 얻었다. 1. 머리너비길이지수는 모두 단두개에 속하였으며, 각 군간에 유의한 차이가 없었다(p>0.05). 2. 1군과 2군의 얼굴지수는 유의한 차이가 없었으나(p>0.05), 1군과 3군간, 2군과 3군간에는 현저한 차이가 있었다(p>0.05). 3. 얼굴지수와 높은 상관관계를 지닌 각 군의 계측항목은 전하안면높이(상관계수: 0.63∼0.69, p>0.001, 총안면높이, 전상치조높이, 전하치조높이 및 총안면두기높이 등이었다. 4. 3군의 두 개계측치는 전면안면높이(상관계수: 0.36∼0.55, p>0.001) 및 전하안면높이(상관계수: 0.31∼0.56, p>0.001)와 높은 상관관계를 나타냈다. 5. 1군과 2군은 얼굴의 수직 계측치가 전반적으로 컸고, 3군은 수평 계측치가 크게 나타나는 경향을 보였다. The purpose of this study was to investigate differences in craniofacial characteristics of professional sportsmen who have practiced since their prepubertal periods. From the standardized lateral and P-A cephalograms of 137 aportsmen, 7 angular, 19 linear, 4 ratio, and 2 index measurements were measured and evaluated by means of statistical methods. The samples were divided into three groups: Group 1; ice hockey(n=17), football(n=27), basketball(n=16), Group 2; baseball(n=16), gymnastics(n=13), and Group 3; judo(n=18), ssireum(n=10), weight lift(n=20). The results were as follows: It seemed obvious that the cephalic indices of the 3 groups exhibited brachycephalic headform (Group 1; 0.85±0.04, Group 2; 0.85±0.04, Group 3; 0.83±0.06) and there was no statistical difference among the groups(p>0.05). The facial indices of the Group 1 (0.93±0.05) and Group 2 (0.93±0.04) exhibited difinite leptoprosopic facial forms while the Group 3 (0.90±0.04) showed more or less euryprosopic facial form, and there appeared significant difference between the Group 1 and 3 (p<0.05), and also between the Group 2 and 3 (p<0.05). There appeared strong relationships between the facial indices and the facial axis angle, mandibular plane angle, total craniofacial height, total facial height, upper anterior dental height, lower anterior dental height, mandibular length, lower anterior facial height ratio, and especially with lower anterior facial height (p<0.01). It seemed that more of the vertical facial measurements of the Group 1 and 2 appeared to be larger than those of the Group 3.

백서의 실험적 치아이동시 connexin 43의 발현에 관한 연구

임정현,강경화,이종진,김은철,김상철 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.5

치아이동시 골세포간 활성 조화에서 세포간 교통의 중요성이 차츰 인식되고 있으며 세포간 교통을 위한 교통반의 존재가 확인되고 있다. 동물실험 모델에서 실험적 치아이동을 통해 조성된 압박 및 견인측 치주인대에서 교통반 단백의 일종인 connexin 43의 발현을 관찰함으로써 인접 연조직 및 골 조직 내에서 세포 신호 전달 양상의 한 부분을 파악하고자 하였다. Sprague-Dawley계 백서 27마리를 대조군(3마리)과 실험군(24마리)으로 나누었으며, 실험군은 견인력(75g)을 가한 후 12시간, 1일, 4일, 7일, 14일, 28일이 경과한 후 각각 4마리씩 희생시켜, connexin 43의 발현을 면역조직화학적으로 관찰한 바 다음과 같은 결과를 얻었다. 1.대조군에서의 connexin 43 발현은 치은, 상아질, 치주인대, 치수, 골세포에서 경미하였다. 2.교정적 치아이동에 반응하는 치수, 치주인대, 골모세포, 파골세포, 골세포부위에서 connexin 43의 발현이 많았으나 치은, 상아질, 상아모세포에서의 발현은 견인력 적용기간에 관계없이 대조군과 큰 차이가 없었다. 3.치수에서 connexin 43의 발현은 교정력을 가한 4일에서부터 증가하여 7일째까지 크게 증가한 후 14일째부터 감소되었으며 28일째에는 대조군과 차이가 없었다. 4.치주인대에서 connexin 43의 발현은 주로 혈관을 중심으로 치조골 면을 따라 압박측에서 주로 나타났는데 교정력을 가한 12시간에서부터 증가하여 4일에서 가장 많은 발현을 보인 후 7일부터 감소하기 시작하였다. 5.골모세포 및 골세포에서 발현은 실험 1일째부터 출현해 4일째에 가장 많은 발현을 보였고 7일 이후에는 크게 감소하였으며 파골세포에서의 발현은 골모세포와 골세포보다 실험 4일째에 더 많이 관찰할 수 있었다. Bone remodeling in response to force requires coordinated actions of osteoblasts, osteoclasts, osteocytes, and periodontal ligament cells. Coordination among these cells may be mediated, in part, by cell-to-cell communication via gap junctions. This study was designed to evaluate the expression of gap junction, connection 43 in periodontal tissue during the experimental movement of rat's incisors, by LSAB(labelled streptavidine biotin) immunohistochemical staining fort connexin 43. Twenty seven Sprague-Dawley rats were divided into a control group(3 rats), and 6 experimental groups(24 rats) where 75g of force was applied from helical springs across the maxillary incisors. Rats of experimental groups were sacrificed at 12 hours, 1, 4, 7, 14 and 28 days after force application, respectively. And the tissues of a control group and experimental groups were studied immunohistochemically. The results were as follows : 1.In control group, the expression of connexin 43 was rare in gingiva, dentin, cementum, periodontal ligament and bone cells. 2.In experimental group, the expression of connexin 43 was increased in pulp, periodontal ligament, osteoblasts, and osteoclasts, comparing to that in control. And it was rare in gingiva, dentin, and odontoblasts regardless of the duration of force application, which was not different from that of control group. 3.The expression of connexin 43 in pulp of experimental group began to increase in 4-day after force application and got to the highest degree at 7-day. And it decreased after 14-day to be similar to that of control group at 28-day. 4.The expression of connexin 43 in periodontal ligament was noted in small capillaries adjacent to alveolar bone, showing higher intensity of immunolabelling after 4-day. And it was stronger in the pressure side than in tension side of periodontal ligament. After 7-day, decrease in connexin 43 expression was observed. 5.The expression of connexin 43 in alveolar bone began to increase 1-day, reached to the highest degree at 4-day, and decreased at 7-day. And the expression in osteoclasts was more than that in osteoblasts or osteocyte at 7-day.

Sertoli 세포의 생리기능 및 호르몬에 의한 조절

임규,박청,윤경아,윤은진,박종일,박승길,황병두 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

금속-도재 수복물에서 비귀금속 합금의 재사용에 따른 결합강도에 관한 연구

임시덕,이은경,장기환 한국위생과학회 2001 한국위생과학회지 Vol.7 No.2

치과용 비귀금속 합금 중 Verabond와 RecilliumⅢ,Ⅴ를 사용하여, 새 금속만 사용한 group(Ver-1, Rex3-1, Rex5-1)과 재사용한 group(Ver-2, Rex3-2, Rex5-2)의 경도측정을 한 결과 새금속만을 사용한 group에서는 Ver-1이 경도측정치가 가장 높았으며, 재사용한 group에서는 Rex5-2가 가장 높은 경도 측정치를 나타내었고 Rex3에서는 Rex3-1과 Rex3-2간의 경도 차이는 없었다. Verabond와 RexolloumⅢ,Ⅴ를 사용한 것과, 새 금속만을 사용하여 Vita Omega Porcelain을 올려서 소성하고, 금속과 도재간의 결합강도를 측정 한 것과, 한번 주조된 비귀금속 합금에 50%의 새로운 합금을 사용하여 Vita Omega Porcelain을 올려서 소성하고, 금속과 도재간의 결합강도를 측정한 결과 재 사용한 금속 중 Rex5-1이 금속과 도재간의 결합강도가 가장 좋은 것으로 나타났고, 주조된 합금에 새 금속 50%를 섞은 group에서는 Rex5-2가 결함강도가 가장 낮은 것으로 나타났다. 그러나 Ver-1과 Ver-2는 금속과 도재간의 결합강도는 큰 차이점이 없는 것으로 보인다. The hardness of non-precious dental alloy was measured using Verabond and Rexillium Ⅲ,Ⅴ, The dental alloy was group into a new alloy group (Ver-1, Rex5-1) and used alloy (Ver-2, Rex5-2). As a result, Ver-1 showed the highest hardness value in a new alloy group. and Rex5-2 showed the highest hardness value in a used alloy group. However, Rex3-1 and Rex3-2 did not show any difference in hardness. The bonding strength between alloys used Verabond and Rexillium Ⅲ,Ⅴ was tested; The bonding strength between porcelain and a new alloy, which was firing after build up weth Vita Omega porcelain; was tested; Also, the bonding strength between porcelain and metal, which was casted and firing after build up with vita Omega pocelain was tested. I this case, the casted alloy was mixed with a new alloy by hay in weight. As a result, Rex5-1 yielded the highest strength among the used alloy group. Rex5-2 was the lowest in bonding strength among the alloy mixed with casted alloy and a new alloy by hay in weight. On the other hand, Ver-1 and Ver-2 did not show apparent difference in bounding strength.