heritance of Glutamate - Oxalate Transaminase and Leucine Aminopeptidase , and Linkage Relationship Among Allozyme Loci

Kim, Zin Suh,Hong, Yong Pyo 한국유전학회 1982 Genes & Genomics Vol.4 No.2

Megagametophyte and embryo tissue of Pinus densiflora were subject to study the inheritance of glutamate-oxalate transaminase (GOT) and leucine aminopeptidase (LAP), and linkage relationship among isozyme loci coding both enzymes, by starch gel zone-electrophoresis. No variation was found in the fastest migrating zone (GOT-A). Electrophoretic phenotypes of the two zones (GOT-B and GOT-C) showed an 1:1 segregation ratio, suggesting that each is controlled by a single locus. Four and three alleles were identified at both loci, respectively. The fourth zone (GOT-D) migrated cathodally displayed isozyme pattern coincided precisely with that of GOT-C. Whether both zones are controlled by the same locus or they represent two tightly linked loci, remained unknown. In all three variant GOT zones, heterozygous embryos produced triple band patterns, indicating that GOT isozyme in Pinus densiflora is a dimer. The segregation of two zones (LAP-A and LAP-B) of activity stained for leucine aminopeptidase suggested that two loci control each of both isozymes. Two and three alleles were identified at both loci, LAP-A and LAP-B. One pair of loci, GOT-B and LAP-B, was found to be tightly linked showing an average recombination frequency of 12.5 percent. Deviation from independent assortment were observed for one other pair of loci, GOT-B and GOT-C.

Distribution of 1-SSR and mtDNA Variants in 19 Populations of Pinus densiflora in Korea, China and Russia

( Zin Suh Kim ),( Ren Qing Wang ),( Peter G. Gorovoy ),( Hae Yun Kwon ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.2

N/A I-SSR variants were analyzed to estimate level and distribution of genetic diversity in 19 populations of Japanese red pine in Korea (10 populations), China (6 populations), and Russia (3 populations). A total of 66 I-SSR variants were observed in the analyzed 551 individuals. All the analyzed individuals could be identified by DNA fingerprints-like individual specific amplicon profiles. Relatively higher level of genetic diversity within populations was observed in 19 populations of Japanese red pine (mean of 0.396) than other tree species. From the results of AMOVA with all 19 populations, relatively high degree of genetic differentiation (φ^(ST)=O. 199) was allocated among populations although majority of genetic diversity was distributed within populations (80.01%), After dividing 19 populations into 3 geographic groups, the degree of population differentiation within groups was lowered to 0.153. The degree of population differentiation was decreased more to φ^(ST)=0.135 by including only 10 Korean populations. Genetic relationships among 19 populations, reconstructed by UPGMA method, revealed 2 weak genetic groups. Three Russian populations were grouped with the most distantly located Chinese population of Xu Gou instead of 2 closely located Chinese populations of Mu Dan Jiang and Long Jing. The overall genetic relationships among the 19 populations were not coincided with geographic affinity. The discrepancy between genetic relationships and geographical distribution among the populations suggested that the analyzed populations might be undergone random changes in genetic composition. The probable cause of the random changes was discussed. PCR-RFLP analyses of mtDNA revealed no variation among 57 individuals selected from 19 populations. Results obtained in this study suggested that more careful approach should be made for preparing strategy for gene conservation of Japanese red pine in Korea, China, and Russia.

Inheritance and Linkage of Some Polymorphic Isozymes in Ginkgo biloba L.

(Zin Suh Kim),(Hae Yeun Kwon) 한국임학회 2000 한국임학회 학술발표논문집 Vol.2000 No.-

강원ㆍ경북지역 소나무 天然集團의 遺傳的 構造

Zin Suh KIM(金眞水),Seok Woo LEE(李錫雨) 한국육종학회 1992 한국육종학회지 Vol.24 No.1

We have studied the genetic variation patterns at 23 loci coding for 16 enzymes in eight natural populations of Pinus densiflora from Kangwon-Kyungbuk region which has been classified into P. densiflora for. erecta. The average proportion of polymorphic loci per population (P) was 75.6% (<0.99). The average number of alleles per locus was 2.3. The averaged observed and expected heterozygosities(Ho, He) showed the same value of 0.249. All investigated values of 8 populations in Kangwon-Kyungbuk region were less than those of 17 natural populations sampled from the other region(P=82.4%, A/L=2.4, Ho=0.263, He=0.268), but showed no statistically significant differences except the value for P. The absence of marker alleles specific to P. thunbergii and the very similar allele-frequency distributions to those of other populations in Pinus densiflora didn’t support the hypothesis that pines in Kangwon-Kyungbuk region are introgressive hybrid between P. densiflora and P. thunbergii. Only a small amount of observed genetic diversity appeared to be interpopulational(Gst=0.042). The mean genetic distance(D) over all pairs of 8 populations was 0.007. A pooled cluster analysis of 25 populations didn’t show any remarkable tendency of genetic differentiation between two groups, 8 populations in Kangwon-Kyungbuk region and 17 other populations of P. densiflora. Population ‘Pongwa’ possessing the smallest amount of genetic variation was separated from the other populations. As a whole, the genetic structure of the natural populations of P. densiflora in Kangwon-Kyungbuk region is not sufficiently distinctive from that of other populations. So it is considered that they cannot be treated genetically as a separate unit, Pinus densiflora for. erecta.

Inheritance of Leucine Aminopeptidase and Glutamate - oxalate Transaminase Isozymes in Pinus koraiensis

Zin Suh Kim,Won Ha Son,Young Koun Youn 한국임학회 1982 한국임학회 학술발표논문집 Vol.1982 No.-

Using techniques of starch gel zone-electro-phoresis, market variability was detected in the leucine aminopeptidase (LAP) and glutamateoxalate transaminase (GOT) isozyme patterns found in the hapolid endosperm of dry Korean Pine seeds (Pinus Koreiensis S. et Z. LAP-B) of activity on gels stained for leucine aminopeptidase suggests that two loci control each of these isozymes. Electrophoretic variants in each zone segregate randomly with respect to the other zone suppeor this hypothesis. three zones of activity are observed for glutamate-oxalate transaminase. No variations was found in the fasted migrating zone (GOT-A). The other tow zones (GOT-B and GOT-C) display a segregation pattern that ciomcides precisely each other. Whether both zones are controlled by the same locus or whether they represent two tightly linked loci remained unknown at present. Electrophortic variants in GOT-B segregate as alleles at single locus. No evidence of genetic linkage was observed between pairs of loci that were heterozygous in the same mother tree

유럽 너도밤나무 ( Fagus sylvatica L . ) 유묘발달 (幼苗發達) 동안의 한 동위효소 유전자좌에서의 (遺傳子座) 생존력선택

김진수 ( Zin Suh Kim ) 한국산림과학회 1981 한국산림과학회지 Vol.54 No.1

The genetic structures at one leucine aminopeptidase locus (LAP-A) of acorns, seedling raised in greenhouse and forest form the two beech provenances. West Germany and Rumania, were investigated and compared with each other. In many pair wise comparisons significant differences in genotypic structure as well as genic structure were ascertained between different developmental stages. In both the provenances, the allele A₂ seems to have advantage at both seedling stages raised under two different conditions. Homozygous carriers of A₂ allelle survived best in greenhouse, while heterozygous carriers especially with A₂ allele possessed great viability under more variable environmental conditions. Since a distinct different genetic background was present in two base populations. the identical effect of the allele A₂ confirms the adaptiveness of this locus. With aid of some measures such as viability parameter and genetic distance, the character of occurred viability selection of further explained. The possible significance of this locus at this early stage is discussed in relation to adaptation of this long lived tree species to heterogeneous environment

금강소나무 - 유전적으로 별개의 품종으로 인정될 수 있는가 ? - 동위효소분석 결과에 의한 고찰 -

김진수(Zin Suh Kim),이석우(Seok Woo Lee),황재우(Jae Woo Hwang),권기원(Ki Won Kwon) 한국산림과학회 1993 한국산림과학회지 Vol.82 No.2

The genetic variation patterns at 23 loci coding for 16 isozymes in eight natural populations of Pious densiflora for. erects distributed in Kangwon-Kyungbuk region and 17 populations of Pious densiflora and 13 populations of Pious thunbergii were compared. The absence of marker alleles specific to P. thunbergii and almost the same allele-frequency distributions to those of P. densijlora did not support the hypothesis that P. densiftora for erects is a introgressive hybrid between P. densiflora and P. thunbergii . From the results of the hierarchial analysis of population differentiation using Wright`s F statistics(1978i. the frequency distributions of single-locus distance coefficients and other genetic analysis (genetic distance. cluster analysis, factor analysis, resin duct analysis), it was concluded that Pious densiflora for. erects cannot be treated genetically as a distinct group from other natural populations of P. densiflora.

캘리포니아 폐구와 소나무류의 엽록체 DNA rbcL 과 atpB 유전자 사이에 존재하는 염기 서열

김진수,홍용표 한국유전학회 1992 Genes & Genomics Vol.14 No.2

The intergenic sequence between rbcL and atpB genes of chloroplast DNA were sequenced from five individual trees, one individual each of Monterey pine (Pinus radiata), knobcone pine (Pinus attenuata), and three geographic groups of bishop pine (Pinus muricata). No variation was observed among the 667 bp intergenic sequences of five individuals which had been directly sequenced with the PCR(polymerase chain reaction)-amplified DNA. Alignment of the upstream sequences (251 bp) of the rbcL gene with those of Douglas-fir showed about 88.4% similarity.

은행나무의 몇가지 다형적 동위효소의 유전양식 및 연관

김진수,권해연 한국임학회 2000 한국산림과학회지 Vol.89 No.4

Isozyme variants of 15 enzyme systems were analyzed in megagametophytes of Ginkgo biloba L. Five enzyme systems (ADH, G6PD, IDH, MPI, and UGPP) appeared to be monomorphic. Only 11 isozyme zones observed in 10 enzyme systems were polymorphic : ACON-A, FST-B, GDH-A, GOT-B, MDH-B, MDH-C, MNR-A, PGI-B, PGM-A, 6PGD-B and SKDH-B. The segregation ratio and heterogeneity at most polymorphic zones suggested that each isozyme zone was controlled by a single locus with codominant alleles, but significant deviation from 1 : 1 segregation was observed at MDH-B in pooled data. Three pairs of isozyme loci (ACON-A : MDH-B, GOT-B : PGI-B, and MNR-A : SKDH-B) were found to be weakly linked. Recombination frequencies between them ranged from 0.38 to 0.40 (p$lt;0.05).

유전자 다양성의 계층분석을 위한 computer program

김진수,이석우 한국유전학회 1993 Genes & Genomics Vol.15 No.4