Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

( Zhuang Ding ),( Tao Tao ),( Lili Wang ),( Yanna Zhao ),( Huiming Huang ),( Demeng Zhang ),( Min Liu ),( Zhengping Wang ),( Jun Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5

Endophytic fungi are an important component of plant microbiota, and have the excellent capacity for producing a broad variety of bioactive metabolites. These bioactive metabolites not only affect the survival of the host plant, but also provide valuable lead compounds for novel drug discovery. In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi was evaluated against five pathogenic microorganisms. Two strains, Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum), displayed broad-spectrum bioactivities. Our following study emphasizes the isolation, identification and bioactivity testing of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3-5) were isolated, and their structures were elucidated using NMR and MS. Compounds 1 and 2 exhibited inhibitory activity against Escherichia coli. Our findings reveal that endophytic fungi from the rubber tree F. elastica leaves exhibit unique characteristics and are potential producers of novel natural bioactive products.

High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/β-catenin pathway

Shichao Zhuang,Xiaohui Yu,Ming Lu,Yujiao Li,Ning Ding,Yumei Ding 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.6

Objective: High mobility group box 3 (HMGB3) plays an important role in the developmentof various cancer. This study aims to explore whether HMGB3 regulates cervical cancer (CC)progression and elucidate the underlying mechanism. Methods: HMGB3 expression in clinical patients' tumor samples were determined byreal-time quantitative polymerase chain reaction (qRT-PCR) and western blot. HMGB3overexpression/knockdown were used to investigate its function. Cell apoptosis and cyclewere detected by Annexin V/PI staining and flow cytometry. In vivo tumor model was madeby subcutaneous injection of HeLa cells transfected with shRNAs targeting HMGB3 (sh HMGB31) into the flank area of nude mice. Western blot was used to detect the levels ofβ-catenin, c-Myc, and matrix metalloproteinase-7 (MMP-7) in Hela and CaSki cells transfectedwith sh-HMGB3 or shRNAs targeting β-catenin. Results: Both messenger RNA and protein levels of HMGB3 were upregulated in CC tissuesfrom patients. High expression level of HMGB3 had positive correlation with serosalinvasion, lymph metastasis, and tumor sizes in CC patient. Functional experiments showedthat HMGB3 could promote CC cell proliferation both in vitro and in vivo. The expressionlevels of c-Myc and MMP-7 were increased, resulting in regulating cell apoptosis, cell cycle,and activating Wnt/β-catenin pathway. Conclusions: Our data indicated that HMGB3 may serve as an oncoprotein. It could be used asa potential prognostic marker and represent a promising therapeutic strategy for CC treatment.

Temperature-constrained topology optimization of nonlinear heat conduction problems

Zhuang Chungang,Xiong Zhenhua,Ding Han 한국CDE학회 2021 Journal of computational design and engineering Vol.8 No.4

This paper presents topology optimization of nonlinear heat conduction problems with multiple domains and multiple constraints, including regional temperature and material volume for reducing temperature. Maximum approximation temperatures in the constraint regions are accurately and dynamically calculated, though temperature and temperature-dependent thermal conductivity change with the update of material distribution. A temperature measure with constant error to approximate regional maximum temperature is adaptive to different temperature ranges. A strategy of hole nucleation generation combined with the regional temperature constraints is presented for the level set-based topology optimization. The solid isotropic material with penalization (SIMP) and parametrized level set methods are compared for the temperature-constrained topology optimization. Finally, several numerical examples are solved by the SIMP and parametrized level set methods. The results demonstrate that the proposed approach can obtain intricate topological details and reduce regional temperatures for the nonlinear heat conduction problems.

Overall evaluation of cutting forces and surface integrity in flank milling of Inconel 718

Kejia Zhuang,Dahu Zhu,Xiaoming Zhang,Han Ding 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

Dual Targeting-Integrated Nanogel with On-Demand Drug Delivery Inhibits Progression of Highly Metastatic Malignancy

Jianxun Ding,Jinjin Chen,Weiguo Xu,Xiuli Zhuang,Xuesi Chen 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

pH-Responsive Drug Delivery Systems Based on Clickable Poly(L-glutamic acid)-Grafted Comb Copolymers

Jianxun Ding,Xuesi Chen,Chaoliang He,Chunsheng Xiao,Jie Chen,Xiuli Zhuang 한국고분자학회 2012 Macromolecular Research Vol.20 No.3

Five pH-responsive alkyne-poly(2-aminoethyl methacrylate)-graft-poly(L-glutamic acid) (alkyne-PAMA-g-PLGA) comb copolymers were synthesized through the ring-opening polymerization (ROP) of γ-benzyl-L-glutamate N-carboxyanhydride (BLG NCA) and the subsequent deprotection of benzyl group from BLG unit. The chemical structures of copolymers were confirmed by proton nuclear magnetic resonance spectra (1H NMR) and Fourier transform infrared spectroscopy (FTIR). The pyrene-probe-based fluorescence technique and transmission electron microscopy (TEM) measurements revealed that the comb copolymers could spontaneously self-assemble into micellar or vesicular nanoparticles in phosphate buffered saline (PBS) at pH 7.4. Doxorubicin (DOX), an anthracycline anticancer drug, was loaded into nanoparticles as a model anticancer drug. The in vitro release results showed that the release behaviors could be altered by adjusting the composition of the comb copolymer and pH of the release medium. In vitro methyl thiazolyl tetrazolium (MTT) assays demonstrated that the copolymers were biocompatible,and DOX-loaded nanoparticles showed effective inhibition of cellular proliferation. Hemolysis tests indicated that the copolymers were also hemocompatible, and that the presence of the copolymers could reduce the hemolysis ratio (HR) of the DOX significantly. In addition, the comb copolymers could be modified through versatile Cu(I)-catalyzed “click chemistry” between the terminal alkyne group and azide-modified functional agents. These properties indicate that the pH-responsive clickable comb copolymers are promising candidates for multifunctional nanocarriers in cancer diagnosis and therapy.

Effect of Surface Waviness on the Performances of an Aerostatic Thrust Bearing with Orifice-Type Restrictor

Hui Zhuang,Jianguo Ding,Peng Chen,Yu Chang,Xiaoyun Zeng,Hong Yang,Xingbao Liu,Wei Wei 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10

In this paper, four waviness types are presented to investigate the effect of surface waviness on the static performance, dynamic performance and stability of an aerostatic thrust bearing with orifice-type restrictor. The influences of wave amplitude and rotational speed on the bearing performances are studied. The Reynolds equation is first solved through small perturbation method to obtain the static and dynamic characteristics. Subsequently, by adopting an iterative method based on the acquired dynamic coefficients, a numerical method is proposed to analyze the bearing stability. The calculated critical supply pressure is compared with that obtained by the traditional orbit method and it indicates that the proposed method has higher computational efficiency. The results show that the surface waviness has great influence on the bearing performances and the effect becomes increasingly pronounced with the growth of wave amplitude. The performances are more sensitive to the waviness arranged in bearing radial direction than circumferential direction. The enhancement of certain performance is always accompanied by the decline of another performance for the bearing with wave pattern on the surface, which leads to the fact that the bearing performances cannot be comprehensively improved in static characteristic, dynamic characteristic and stability. Besides, high rotational speed will cause pneumatic instability more readily. Finally, a stiff ness measuring experiment and a modal test are performed to verify the accuracy of computational analysis and results.

Research on Whole‑Process Tensile Behavior of Headed Studs in Steel–Concrete Composite Structures

Liang?Dong Zhuang,Hong?Bing Chen,Yuan Ma,Ran Ding 한국콘크리트학회 2021 International Journal of Concrete Structures and M Vol.15 No.4

The headed studs have been widely applied in steel–concrete composite structures as shear connectors. However, the tensile performance of headed studs is also key to the structural performance in many cases such as the semi-rigid composite joints including steel beam–concrete wall joint and steel column–base joint. Therefore, this study presents experimental and analytical study on the whole-process tensile behavior of headed studs. Tests on a total of 33 pullout specimens are first conducted. The tensile capacity and load–deformation behavior of the anchorage concrete, which dominates the structural performance of headed studs, are thoroughly analyzed. In addition, test data in the literature are collected for quantitatively evaluating the influence of embedment depth, bearing area, boundary conditions, and concrete strength on the tensile behavior of the anchorage concrete. On the basis of the influence evaluation, an analytical model represented by a piecewise function is proposed to describe the whole-process load–deformation behavior of the anchorage concrete and validated through the comparison between the predicted curves and all collected experimental results. Then the proposed model is applied to simulate the rotational behavior of the typical semi-rigid joint anchored by headed studs, which takes the contribution of the anchorage concrete into consideration, and is verified by experimental results. The research findings indicate that tensile behavior of anchorage concrete is crucial to the structural performance of semi-rigid joints, even for headed studs with large embedment depth and bearing area.

RNA Interference Targeting Nucleocapsid Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication in Marc-145 Cells

Minnan Yang,Zhuang Ding,Qun Xiang,Xiaodong Zhang,Xiang Li,Seydou Sylla 한국미생물학회 2014 The journal of microbiology Vol.52 No.4

Porcine reproductive and respiratory syndrome (PRRS) is animportant disease, which leads to severe economic losses inswine-producing areas of the world. However, current antiviralstrategies cannot provide highly effective protection. In this study, three theoretically effective interference targetsites (71-91, 144-164, 218-238) targeting the nucleocapsid (N)gene of PRRSV were designed and selected, and then threesiRNA-expressing plasmids were constructed, respectivelynamed p2.1-N71, p2.1-N144, and p2.1-N218. The recombinantsiRNA-expressing plasmids were transfected into Marc-145 cells; then the cells were infected with PRRSV (JL07SWstrain); finally, after incubation for 48 h, the antiviral activityof those siRNA-expressing plasmids in Marc-145 cells wasassessed by cytopathic effects, virus titers, indirect immunofluorescence,and quantitative real-time PCR. Experimentalresults demonstrated that these three siRNA-expressing plasmidscould effectively and significantly inhibit the replicationof PRRSV by 93.2%, 83.6%, and 89.2% in Marc-145 cells,respectively. Among these three siRNA-expressing plasmids,p2.1-N71 was found to be most effective, while p2.1-N144and p2.1-N218 displayed relatively weak inhibition of virusreplication. The results indicated that siRNA-expressing plasmidstargeting the N gene of PRRSV could significantly inhibitPRRSV replication in Marc-145 cells. Based on our experimentalresults and previous reports, the 71-91, 179-197,and 234-252 sites of the N gene are good choices to effectivelyinhibit the replication of PRRSV, and this RNA interferencetechnique can be a potential anti-PRRSV strategy.

Effect of fermented blueberry on the oxidative stability and volatile molecule profiles of emulsion-type sausage during refrigerated storage

Zhou, Hengyue,Zhuang, Xinbo,Zhou, Changyu,Ding, Daming,Li, Chunbao,Bai, Yun,Zhou, Guanghong Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.5

Objective: The aim of this work was to assess the effect of fermented blueberry (FB; 2%, 4%, and 6%) on the oxidative stability and volatile molecule profiles of emulsion-type sausage stored at 4℃ for 28 days. Methods: The antioxidant activity of FB was determined through radical-scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. Four formulations of sausage treatments with different FB levels (0%, 2%, 4%, 6%) were prepared, then peroxide value (POVs), thiobarbituric acid-reactive substances (TBARS) values, protein carbonyls and thiol groups were measured. The aroma profiles of sausages for each treatment was also determined. Results: The half maximal inhibitory concentration indicated that FB had greater scavenging ability than ascorbic acid against DPPH and hydroxyl radicals. Sausages with FB significantly retarded increases in POVs and TBARS, as well as in the content of protein carbonyls during all storage days (p<0.05). Particularly, 4% and 6% FB-treated sausages had better oxidation inhibition effects. However, FB accelerated the reduction in thiol groups (p<0.05). Additionally, FB inhibits the excessive formation of aldehyde compounds; for example, hexanal, which may cause rancid flavors, decreased from 58.25% to 19.41%. FB also created 6 alcohols (i.e., 2-methyl-1-propanol, 3-methyl-1-butanol, and phenylethyl alcohol), 5 ester compounds (i.e., ethyl acetate, ethyl lactate, and ethyl hexanoate) and 3-hydroxy-2-butanone in the sausages that contribute to sausage flavors. The principal component analysis showed that the aroma profiles of sausages with and without FB are easily identified. Conclusion: The addition of FB could significantly reduce the lipid and protein oxidation and improve oxidative stability for storage. Also, adding FB could inhibit rancid flavors and contribute to sausage flavors.