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Radiation Hybrid Mapping and mRNA Expression of Chicken N- myc downregulated gene 4
Yong Tian,Li Zhi Lu,Yan Fu,Ai Ping Yuan,Guo Qin Li,Qing Yan Yuan,Zheng Rong Tao,Jin Zhao 한국유전학회 2007 Genes & Genomics Vol.29 No.4
N-myc downregulated gene 4 (NDRG4) is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscle cells. To map NDRG4 gene in chicken chromosome, a 6,000 rads chicken- hamster radiation hybrid panel was used. Primers were designed according to the published human sequence for amplification of chicken NDRG4. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken NDRG4, and found that the assembled contig shared a high percentage of similarity with that of human gene. PCR of samples from ChickRH6 revealed the locations of NDRG4 to be linked to the maker PARD6G (5 cR away) with a LOD score 20.46. In addition, we detected the mRNA expression and distribution of chicken NDRG4 in various tissues by RT-PCR, and found that NDRG4 was highly expressed in chicken brain and heart, whereas lowly but detectable in thymus. The mRNA expression of this gene in chicken liver, spleen, lung and muscle was rarely detectable under present experimental conditions.
Variation of Urinary and Serum Trace Elements (Ca, Zn, Cu, Se) in Bladder Carcinoma in China
Guo, Kun-Feng,Zhang, Zhe,Wang, Jun-Yong,Gao, Sheng-Lin,Liu, Jiao,Zhan, Bo,Chen, Zhi-Peng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Backgrounds: Deficiency or excess of trace elements can induce body metabolic disorders and cellular growth disturbance, even mutation and cancerization. Since there are few studies of the effect of trace elements in bladder carcinoma in China, the aim of this study was thus to assess variation using a case control approach. Methods: To determine this, 81 patients with bladder carcinoma chosen as a study group and 130 healthy persons chosen as a control group were all assayed for urinary and serum trace elements (calcium [Ca], zinc [Zn], copper [Cu], selenium [Se]) using an atomic absorption spectrophotometer, and the results were analyzed by independent sample t tests. The correlative factors on questionnaires answered by all persons were analyzed by logistic regression. Results: The results showed urinary Ca, Zn and serum Cu levels of the study group to be significantly higher (P<0.05) than those of he control group. Serum Ca and Se levels of study group were significantly lower (P<0.05) than those of control group. Conclusion: There were higher urinary Zn and serum Cu concentrations in bladder carcinoma cases. Bladder carcinoma may be associated with Ca metabolic disorder, leading to higher urinary Ca and lower serum Ca. Low serum Se and smoking appear to be other risk factors for bladder carcinoma in China.
Large-Scale Dataset Incremental Association Rules Mining Model and Optimization Algorithm
Guo Yu-Dong,Li Sheng-Lin,Li Yong-Zhi,Wang Zhao-Xia,Zeng Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.4
Mining association rules is an important research direction in the field of data mining. Related studies have proposed many used to efficiently find large-scale database association rules algorithm, but the research on maintenance problem of association rules is less. Especially many transaction database is always in constant updates. Increase or decrease occurs when the database or dataset minimum support after the change, how to maintain the association rules have been, it got the attention of many researchers. Based on IFP-Growth increment of association rules mining model and to modify the FP-tree, put forward the suitable for transaction data and support the tree model of change, at the same time under different conditions is given incremental association rules mining algorithm, and reduce the frequency of the original dataset range query and query, and in a case of massive dataset multi-level tree structure decomposition, dynamic allocation rule tree branches, ensure load balancing, improve operation efficiency.
Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Yong Zhi,Shunmei Lin,A-Yeung Jang,Ki Bum Ahn,Hyun Jung Ji,Hui-Chen Guo,임상용,Ho Seong Seo 한국미생물학회 2019 The journal of microbiology Vol.57 No.1
Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Since glucose is crucial for intracellular survival and replication in host cells, we constructed strains with mutations in components of the glucose uptake system, called the phosphotransferase system (PTS), and compared the relative virulence and immune responses in mice. In this study, we found that the strain with mutations in both ptsI and crr (KST0556) was the most attenuated strain among the tested strains, and proved to be highly effective in inducing a mucosal immune response that can protect against NTS infections in mice. Thus, we suggest here that KST0556 (ΔptsIΔcrr) is a potential live vaccine candidate for NTS, and may also be a candidate for a live delivery vector for heterologous antigens. Moreover, since PTS is a well-conserved glucose transporter system in both Gramnegative and Gram-positive bacteria, the ptsI and crr genes may be potential targets for creating live bacterial vectors or vaccine strains.
Xue-Yong Zhang,Ying-Na Jian,Hong Duo,Xiu-Ying Shen,Yi-Juan Ma,Yong Fu,Zhi-Hong Guo 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.4
Coenurosis is an important zoonotic helminthic disease caused by the larval stage of the tapeworm Taenia multiceps. This parasite typically infects the brain of the intermediate hosts, including sheep, goat, cattle and even hu- mans. We report a case of T. multiceps infection in a yak confirmed by clinical symptoms, morphological characteristics, and molecular and phylogenetic analyses. The coenurus was thin-walled, whitish, and spherical in shape with a diameter of 10 cm. The parasite species was identified as T. multiceps by PCR amplification and sequencing of the 18S rRNA, cox1 and nad1 genes. Three gene sequences all showed high homology (all above 97%) with the reference sequences from different hosts. Moreover, phylogenetic reconstructions with the 3 published Taenia gene sequences confirmed that the Qinghai yak isolate was closely related to T. multiceps. Although there are advanced diagnosis and treatment meth- ods for coenurosis, early infection is difficult to diagnose. Importantly, the findings of yak infection case should not be ig- nored due to its zoonotic potential.
Yang Zhi Hong,Jin Hua,Plaha Prikshit,Woong Bae Tae,Jiang Guo Bin,Woo Jong Gyu,Yun Han Dae,Lim Yong Pyo,Lee Hyo Yeon The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.4
We studied the effect of genotype, explant, age of explant, medium (plant growth regulators and gelling agents), and standardized an efficient regeneration protocol for inbred lines of Chinese cabbage (Brassica rapa ssp. pekinensis). Of the different concentrations of NAA and BA tested, the combination of $5\;\cal{mg/L}\;BA\;and\;0.5\;\cal{mg/L}$ NAA gave the highest frequency of shoot regeneration. The cotyledonary explants had more shoot regeneration frequency ($\ge40\%$) than the hypocotyl ex-plants. Besides, the cotyledonary explants, excised from the 4-day old seedlings, showed higher shoot regene-ration ($56.7\%$). Of the three gelling agents and their concentrations used, 16 g/L agar was found to be the best for shoot regeneration. Shoot regeneration frequency in-creased significantly by supplementing the medium with $4\;\cal{mg/L}\;of\;AgNO_3$ MS medium devoid of NAA showed higher frequency of rooting in the regenerated shoots than the ones supplemented with NAA. Our improved regeneration protocol will be especially useful for the genetic transformation of Chinese cabbage inbred lines to develop transgenic hybrids.
Zhong, Zhi-Rong,Zhang, Zhi-Rong,Liu, Ji,Deng, Yong,Zhang, Hong-Wei,Fu, Yao,Song, Qing-Guo,He, Qin 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf-PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of $240{\pm}12nm$ and the zeta potential was $-24.10{\pm}2.5mV$. The transfection efficiencies of PLPD and Tf-PLPD were $12.18{\pm}3.8\;and\;24.26{\pm}2.6mU\;{\beta}-galactosidase/mg$ protein respectively. The lyophilization and the presence of serum didn't affect the tansfectivities of PLPD or Tf-PLPD. Compared to $Lipofectamine^{TM}$ 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Wang, Zhi-shen,Li, Yong,Zhu, Wei-shen,Xue, Yi-guo,Jiang, Bei,Sun, Yan-bo Techno-Press 2017 Geomechanics & engineering Vol.12 No.4
Due to high in-situ stress and brittleness of rock mass, the surrounding rock masses of underground caverns are prone to appear splitting failure. In this paper, a kind of loading-unloading variable elastic modulus model has been initially proposed and developed based on energy dissipation principle, and the stress state of elements has been determined by a splitting failure criterion. Then the underground caverns of Dagangshan hydropower station is analyzed using the above model. For comparing with the monitoring results, the entire process of rock splitting failure has been achieved through monitoring the splitting failure on side walls of large-scale caverns in Dagangshan via borehole TV, micro-meter and deformation resistivity instrument. It shows that the maximum depth of splitting area in the downstream sidewall of the main power house is approximately 14 m, which is close to the numerical results, about 12.5 m based on the energy dissipation model. As monitoring result, the calculation indicates that the key point displacement of caverns decreases firstly with the distance from main powerhouse downstream side wall rising, and then increases, because this area gets close to the side wall of main transformer house and another smaller splitting zone formed here. Therefore it is concluded that the energy dissipation model can preferably present deformation and fracture zones in engineering, and be very useful for similar projects.
Molecular and enzymatic characterization of acid phosphatase from venom of Scleroderma guani
Nai-Yong Liu,Xiao-Hong Fan,Zhi-Quan Zhang,Guo-Xing Wu,Jia-Ying Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Acid phosphatase (ACPase) is a common component in venom of parasitoids. Although extensive researches regarding this enzyme have been conducted in many other organisms, its characteristics as a venomous enzyme are still sparsely known. In this study, we aimed to reveal the gene expression patterns, and structural and biochemical properties of an ACPase from the venom of Scleroderma guani. The cloned open reading frame of venomous ACPase gene of S. guani was 1218 bp encoding 406 deduced amino acids, shared 40% and 41% identities to ACPases from venoms of Apis mellifera and Pteromalus puparum, respectively. The structural analysis of this ACPase implied common functions and differences to the honeybee venom ACPase. qPCR analysis showed that this gene was abundantly expressed in the venom apparatus, and most highly expressed in the adult stage after one and three days emergence. Activity assay using p-nitrophenyl phosphate as the substrate revealed that the optimal pH and temperature for this venomous enzyme was 4.8 and 45 °C, respectively. NaF is an effective inhibitor for it. The results will enrich our knowledge for the ACPase as toxin, which may contribute to further uncovering its role involved in parasitism.