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Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3
Yan, Yutao,Ding, Yu,Ming, Bingxia,Du, Wenjiao,Kong, Xiaoling,Tian, Li,Zheng, Fang,Fang, Min,Tan, Zheng,Gong, Feili Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.5
Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF-${\alpha}$ or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.
Zheng-Fei Yan,Jian Guo,Feng-Hua Tian,Xin-Xin Mao,Yu Li,Chang-Tian Li 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Schisandra chinensis has been used as traditional medicine. The structures of isolate active compounds (schisandrin B, deoxyschisandrin, schisandrin C) from S. chinensis were characterized by physical and spectroscopic analyses. Active compounds were tested for their potential to act as anti-melanogenesis or skin-whitening agents by their abilities to inhibit tyrosinase activity in the cell-free mushroom tyrosinase assay and cellular tyrosinase derived from B16 melanoma cells. The tyrosinase inhibitory activity was correlated to the inhibition of melanin productions in α-MSH-stimulated and unstimulated B16 cells. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver- Burk plot. Schisandrin B was minimally cytotoxic (cell viability: 88.99% at 0.75 μM) and the IC50 value for suppression of mushroom tyrosinase activity was estimated as 0.6 μM. Zymography analysis demonstrated schisandrin B’s concentration-dependent effects and the kinetic analysis indicated schisandrin B’s noncompetitive-inhibitory action.
Yan, Kai,Lee, Hyun-Wook,Gao, Teng,Zheng, Guangyuan,Yao, Hongbin,Wang, Haotian,Lu, Zhenda,Zhou, Yu,Liang, Zheng,Liu, Zhongfan,Chu, Steven,Cui, Yi American Chemical Society 2014 NANO LETTERS Vol.14 No.10
<P>Stable cycling of lithium metal anode is challenging due to the dendritic lithium formation and high chemical reactivity of lithium with electrolyte and nearly all the materials. Here, we demonstrate a promising novel electrode design by growing two-dimensional (2D) atomic crystal layers including hexagonal boron nitride (h-BN) and graphene directly on Cu metal current collectors. Lithium ions were able to penetrate through the point and line defects of the 2D layers during the electrochemical deposition, leading to sandwiched lithium metal between ultrathin 2D layers and Cu. The 2D layers afford an excellent interfacial protection of Li metal due to their remarkable chemical stability as well as mechanical strength and flexibility, resulting from the strong intralayer bonds and ultrathin thickness. Smooth Li metal deposition without dendritic and mossy Li formation was realized. We showed stable cycling over 50 cycles with Coulombic efficiency ∼97% in organic carbonate electrolyte with current density and areal capacity up to the practical value of 2.0 mA/cm<SUP>2</SUP>and 5.0 mAh/cm<SUP>2</SUP>, respectively, which is a significant improvement over the unprotected electrodes in the same electrolyte.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2014/nalefd.2014.14.issue-10/nl503125u/production/images/medium/nl-2014-03125u_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl503125u'>ACS Electronic Supporting Info</A></P>
Altererythrobacter deserti sp. nov., isolated from desert soil
Yan, Zheng-Fei,Lin, Pei,Won, Kyung-Hwa,Yang, Jung-Eun,Li, Chang-Tian,Kook, MooChang,Yi, Tae-Hoo Microbiology Society 2017 International journal of systematic and evolutiona Vol.67 No.10
<P>A Gram-stain-negative, aerobic, short rod-shaped, non-motile bacterium (THG-S3(T)), was isolated from desert soil. Growth occurred at 15-35 degrees C (optimum 28 degrees C), at pH 5-10 (optimum 7) and at 0-4% NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-S3(T) were identified as Altererythrobacter rigui KCTC 42620(T) (99.0 %), Altererythrobacter dongtanensis KCTC 22672(T) (97.1 %), Altererythrobacter xinjiangensis CCTCC AB 207166(T) (96.9 %), Altererythrobacter troitsensis KCTC 12303(T) (96.9 %). Levels of relatedness among strain THG-S3(T) and other Altererythrobacter species were lower than 96.0 %. DNA-DNA hybridization values between strain THG-S3(T) and A. rigui KCTC 42620(T), A. dongtanensis KCTC 22672(T), A. xinjiangensis CCTCC AB 207166(T) and A. troitsensis KCTC 12303(T) were 59.7% (42.8 %, reciprocal analysis), 45.1% (36.3 %), 34.7% (25.1 %) and 15.1% (12.3 %), respectively. The DNA G+C content of strain THG-S3(T) was 69 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified lipids The quinone was ubiquinone-10. The major fatty acids were C-16 : 0, C-17 : 1 omega 6c, C-18 : 1 omega 7c and summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-S3(T) represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter deserti sp. nov. is proposed. The type strain is THG-S3(T) (=KACC 19190(T) = CGMCC 1.15959(T)).</P>