균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

커뮤니티공간으로서의 어린이공원 조성에 관한 연구

김연금,김성주,박환지,유다희,최성용,홍승모,Kim, Yun-Geum,Kim, Seong-Joo,Park, Hwan-Ji,Yu, Da-Hee,Choi, Sung-Young,Hong, Seung-Mo 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6

This study deals with the participatory design process of renovating a children's park. The process should proceed with two major elements. Firstly, the children's park should be designed from the perspective of children. Secondly, it should be designed in consideration of the context of the community. This study is divided into two parts. First is the review of theory concerning the meaning and roles of the children's park as a community space, the importance of children's participation in the design and the participatory method of the children. The second is the case study. The site for the case study is Ssial Park in Nowon-Gu, Seoul. The entire process of the case study is with community participation, from identifying the park's problems to the actual construction. At every step, through the participatory programs, the community participates in the decision making. In addition, mutual understanding among participants is pursued through diverse means of communication. The following results of the case study were found. Firstly, diverse participatory programs and the active trial to enable the community to communicate contribute to the sense of ownership and responsibility concerning the park Secondly, the community can negotiate the differences in opinions without needing the help of experts. Of course, there are limits in the case study, such as the fact that the community organization, which is the core of communication and maintenance concerning the park, is not organized through the process of a case study. Another is that more diverse methods, which inform all community members of the participatory process, should have been used.

편측 협응훈련에 의한 운동신경망의 재조직 및 교차훈련의 효과

박지원,김종만,서정환,김연희 한국전문물리치료학회 2002 한국전문물리치료학회지 Vol.9 No.3

We report the reorganization of motor network resulted from intensive unilateral coordination training and the effect of cross education on the untrained side in patient with traumatic brain injury using functional magnetic resonance imaging (fMRI). A 22 year-old male patient who had suffered from diffuse axonal injury for 58 months showed coordination deficit in the left hand at initial examination. Intensive motor training including complex finger movements and coordination activities using a metronome was introduced to the patient 4 hours per day for a week. FMRI was performed on a 3T ISOL Forte scanner. All functional images were analyzed using SPM-99 software. Hand function was improved after training not only in the trained lefr hand, but also in the untrained right hand. There was no activation in the right primary motor area (M1) during left hand movement before training whereas robust activation of left M1 was demonstrated by the right hand movement. Profuse activation of bilateral prefrontal lobes was seen during both hand movements before training. After training of left hand, right M1 became prominently activated during the left hand motion. The activation of bilateral prefrontal lobes disappeared after training not only for the left hand movement but also for the right, which clearly demonstrated the effect of cross education. This case report demonstrated the learning-dependent reorganization of the M1 and the effect of cross education.

간호학생의 중환자실 임상실습 체험 연구

박경숙,황윤영,최은희 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: The purpose of this study is to investigate the perception of nursing students' on-side clinical experience at intensive care unit(ICU), which will help on developing fundamental tool to enhance the effect of their clinical practice. Method: Van Manens hermeneutic-phenomenological method has been used to analyze the data. participants consisted of 74 third-year nursing students who performed the clinical practice at an ICU in C university hospital. The students got the group interviews and contents of the students clinical diaries. Result: Major concerns related to students clinical practice were found as follows : 「being nervous about unfamiliar machines and situations」,「being frustrated about the patients」,「being stupefied from witnessing a death」,「realizing the importance of health」,「realizing individual patient's precious value through family's love」,「realizing the importance of treating a patient as humanbeing」,「realizing the differences between theory and practice」,「modelizing two aspects of nursing: professional and personal」,「readiness to become a capable nurse」. Conclusion: We are able to obtain in-depth understandings about the nursing students lived experience of clinical practice at the ICU. Based upon this, there is a need to develop a better nursing intervention enhance the effectiveness of the nursing students clinical practice.

반복적 경두부 자기자극이 운동학습과 뇌 운동영역 활성화에 미치는 영향 : 예비연구

박지원,김종만,김연희 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.3

뇌졸중 환자에서 아포지단백 E의 유전자형에따른 혈중 지질과 예후

김연희,박성희,신용일,서정환,김달식 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.4

용액 중합에 의한 전도성 Poly(amide)/Polypyrrole 복합체의 제조와 그 전기적 성질

박연흠,안찬호,한명희 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

Poly(amide)/Polypyrrole composite films were prepared by solution ploymerization method. The films obtained by this method exhibited high conductivities. After polymerization of pyrrole for 1 hr, composite films showed the conductivity as high as 10^-3∼10^-2 S/cm and it increased with increasing the concentration of oxidizing agent. The stability of conductivity to ambient air exposure was excellent. As the result of the thermal characterization of composite films by TGA, we found that the composite film was thermally stable.

Rolling Ball Viscometer에 依한 乳牛의 準臨床型 乳房炎의 感染率에 關한 調査硏究

朴玉潤,曺熙澤 진주산업대학교 1984 論文集 Vol.22 No.-

This study was conducted to find out the infection rate of subclinical type and suspected positive of mastitis of dairy cows using Rolling Ball viscometer. The tested fore milk samples were collected from 1604 teats of 404 Holstein which their somatic cell number had more than 250,000 per mililiter, and these cows were selected from 241 farms located in Kyung­nam province for months from March 1984 to October 1984. The comatic cell number were measured by the viscometer, and the relation between the number of somatic cells of the sample and the cause factors of mastitis were examed, and the results obtained were as follows. 1. The sample which its somatic cell number has less than 250,000 denoted as negative infection was 26.9%, between 250,000 and 490,000 denoted as suspected positive infection was 28.6% and more than 500,000 denoted as positive infection was 24.5%. 2. In the case of the infection rate, 53.5% was infected among 404 cows and 26.0% was infected among 1606 teats. 3. In the test of suspected positive infection and subclinical type according to farm scale, the small scale farm(raising 1 to 10 cows per farm) has 54.7%, the medium scale farm(11 to 20 cows) has 43.5%, the large scale farm(21 to 30 cows) has 50%., and more than 31 cows per farm showed negative infection rate. The less scale farm showed the hiher the % of suspected positive infection. 4. In the case of suspected positive and subclinical type rate according to milking methods, it was 53.8% by milker and 28.6% by hand. 5. In the case of suspected positive and subclinical type rate according to disinfection by soaking the treats into the anticeptic solution after milking, the disinfected cows have 40.5% and nondisinfected cows have 64.8%. 6. In the case of suspected positive and subclinical type rate accoring to the type of administraters, It was 48.1% by ranchman and 89.7% by herd man. 7. In the case of suspected positive and subclinical type rate according to the number of years for raising the cows(personel experiences), It was 44.0% in the case of one year's experience, 45.1% in 2 to 3 years, 58.6% in 4 to 5 years and 69.1% in the case of more than 6 years of experience As the feeding experience increased the infection rate of subclinical type of mastitis was decreased.