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김유진,박미경,박이랑,이보람,이혜림,전선미,양난영,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38
The results of this Study are as follows:33.6% of all participants have insomnia; 22.5% of those who have insomnia are DIS(difficulty in initiating sleep), 17.3% are DMS(difficulty returning to sleep once awakened) and 7.0% awakened too early. 3 4.8% experience sleepiness during daytime. Type 1, experiencing insomnia and sleepi ness during daytime together, is 12.0%, Type 2, with insomnia only, is 21.6%, Type 3, with sleepiness during daytime only, is 22.8% and 43.5% experience no sleeping disturbances. After studying only those with 3 types of sleeping disturbances, it is found that the most common cause of such disturbance is stress 88.4%, anxiety 56.0%, no apparent reason 33.8%, anxiety/fear/terror 29.3%, hurry 23.6%, alcohol/caffeine 16.9%, bedroom tem perature 11.1%, urination during nighttime and persons living together 10.7%, noise from inside 8.9%, illumination 8.0%, and pain/itch 5.8%. The one group revealed significant differences in residential environment(p=0.003). Sex, age, education level, medicine, monthly earning revealed no meaningful differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), whether taking enough sleep(p=0.029), whether taking regular sleep(p=0.005) showed significant differences depending on whether or not having insomnia, and mean duration of sleep time, time to sleep, time of rising, whether taking naps did not reveal significant differences. Of sleep behavior, time to sleep(p=0.000), whether taking naps(p=0.000), indicated significant differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), and whether taking enough sleep(p=0.000), time of going to bed (p=0.002), whether or not taking nap(p=0.000), whether or not taking regular sleep(p=0.010) indicated significant differences among the sleeping disturbance types.
Yu Rim Lee(이유림),Soo Youn Lee(이수연),Ji Yye Lee(이지예),HuiSu Kim(김희수),Jin-Suk Lee(이진석),Sangmin Lee(이상민) 한국신재생에너지학회 2021 한국신재생에너지학회 학술대회논문집 Vol.2021 No.7
Polyhydroxybutyrate (PHB) is interested as a substitute for polypropylene. The effects of growth conditions on PHB production have been widely studied in chemolithotrophs, particularly in Rhodobacter. However, the results on PHB production in Rhodobacter have been inconstant due to different strains and experimental conditions, and it is unclear how diverse environmental factors are connected with PHB production. Here, we report optimized growth conditions for PHB production and show that the growth conditions are closely associated with reactive oxygen species (ROS) regulation. PHB accumulates in cells up to 50% at the highest level under dark aerobic conditions as opposed to light aerobic/anaerobic conditions. According to the time course, PHB contents increase at 48h and then gradually decreased. When observing the effect of temperature and medium composition on PHB production, 30℃ and C/N ratio of 9:1 or more were found to be most effective. Among PHB biosynthetic genes, PhaA and PhaB are highly correlated with PHB production, while PhaC and PhaZ observer little change in overall expression levels. We found that peroxidase activities and expression levels of antioxidant-related genes in cells under dark conditions were relatively high, whereas the amount of H<sub>2</sub>O<sub>2</sub> was comparatively low compared to the light conditions. These results suggest optimal culture conditions for growth and PHB production and the importance of ROS-scavenging signaling with regard to PHB production.
Lee, Sung-Hyun,Park, Junbeom,Kim, Hye-Rim,Lee, Taeseon,Lee, Jaegeun,Im, Yong-O.,Lee, Cheol-Hun,Cho, Hyunjung,Lee, Hyeseon,Jun, Chi-Hyuck,Ahn, Yu-Chan,Lee, In-Beum,Lee, Kun-Hong Elsevier 2016 Carbon Vol.100 No.-
<P>The optimum synthesis conditions for carbon nanotube (CNT) fibers were investigated using the Design of Experiment (DOE) technique. Direct spinning processes are governed by a variety of experimental factors: the methane flow rate, ferrocene flow rate, sulfur flow rate, hydrogen flow rate, water flow rate, and reaction temperature. The process was optimized in two stages that addressed first the Fractional Factorial Design (FFD) and then the Response Surface Methodology (RSM). Results from each experiment were classified according to a 6-step rating system: nothing(1), black gas(2), dust(3), ribbon or film(4), fiber(5), or continuous fiber(6). In the first step, three major factors (methane, sulfur, temperature) were identified as important among the six experimental factors tested using FFD. The effects of the major factors and the interactions were analyzed through the main effect plot and the interaction plot. In the second step, the experimental conditions were optimized using a model equation derived from Box-Behnken design experiments. Finally, the CNT fibers were continuously synthesized under the optimum conditions. The synthesized CNT fibers mainly consisted of single-walled CNTs (SWCNTs) 1.2 -3.8 nm in diameter. The I-G/I-D ratio of the CNT fibers was 48. This work provides a useful methodology for synthesizing the CNT fibers. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Behaviour alteration of Topoisomerase IIIβ knockout mice
Yu Rim Kim,Eun Kyoung Kim,Young Sub Byun,Hae Rim Kim,Ju Sung Kang,Ju Young Lee,Min Young Lee,Sang Mi Cho,Ki Hoon Lee,Hoyoung Ghang,Won Kee Yoon,Young Suk Won,Hyoung Chin Kim,Young Hee Lee,Ki Hoan Nam 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8
Basic phenotyping platform for genetically engineered mouse at KRIBB
Hae Rim Kim,Eun Kyoung Kim,Yong Sub Byun,Kang Ju Seong,Ju Young Lee,Yu Rim Kim,Min Young Lee,Sang Mi Cho,Taek Chang Lee,Hae Jin Lee,Young Ho Kho,Ok Seong Moon,Young Won Seo,Ki Hoon Lee,Hoyoung Ghang,W 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8