Impact of selected fungi from an artificial diet on the growth and development of Drosophila suzukii (Diptera: Drosophilidae)

Huan-Huan Gao,Nan Xu,Hao Chen,Qian Liu,Qian-Yun Pu,Dong-Yun Qin,Yi-Fan Zhai,Yi Yu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Drosophila suzukii (Diptera: Drosophilidae) is one of the very few Drosophila species which are able to lay eggs and feed on healthy ripening fruit. Adults and larvae are all able to obtain the nutrition in the decaying food, which will generate many microorganisms. However, the relationship between D. suzukii and microorganism is poorly understood. In this study, 13 species of fungi were identified in an artificial diet fed by D. suzukii. Short-term and continuously life table experiments were conducted to determine the impact of three of these fungus species including Geotrichum candidum, Talaromyces minioluteus and Actinomucor elegans on the growth and development of Drosophila suzukii. Results revealed that, compared to the control, G. candidum, T.minioluteus and A. elegans increased the mortality of D. suzukii adults in the short time, while extended the developmental time of pupal D. suzukii by 18.00%, 16.22% and 26.44%, respectively in the life table experiment. T. minioluteus reduced the total longevity of D. suzukii by 15.52%, while A. elegans enhanced the total longevity by 25.96%. G. candidum and A. elegans increased the fecundity bymore than two folds. T. minioluteus elongated the mean generation time (T) by 31.34%, whereas G. candidum shortened it by 15.26% but increased the net reproductive rate (R0) by 217.76%, intrinsic rate of increase (r) by 88.89% and finite rate of increase (λ) by 9.17%. It was concluded that G. candidumand A. elegans significantly had the beneficial effect on the growth and reproduction of D. suzukii, however, T. minioluteus had the negative effect on D. suzukii. Our results could provide a new integrated pest management strategy for D. suzukii which would be discussed in this study.

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

Multi-strategy structural damage detection based on included angle of vectors and sparse regularization

Huan-Lin Liu,Ling Yu,Ziwei Luo,Zexiang Chen 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.75 No.4

Recently, many structural damage detection (SDD) methods have been proposed to monitor the safety of structures. As an important modal parameter, mode shape has been widely used in SDD, and the difference of vectors was adopted based on sensitivity analysis and mode shapes in the existing studies. However, amplitudes of mode shapes in different measured points are relative values. Therefore, the difference of mode shapes will be influenced by their amplitudes, and the SDD results may be inaccurate. Focus on this deficiency, a multi-strategy SDD method is proposed based on the included angle of vectors and sparse regularization in this study. Firstly, inspired by modal assurance criterion (MAC), a relationship between mode shapes and changes in damage coefficients is established based on the included angle of vectors. Then, frequencies are introduced for multi-strategy SDD by a weighted coefficient. Meanwhile, sparse regularization is applied to improve the ill-posedness of the SDD problem. As a result, a novel convex optimization problem is proposed for effective SDD. To evaluate the effectiveness of the proposed method, numerical simulations in a planar truss and experimental studies in a six-story aluminum alloy frame in laboratory are conducted. The identified results indicate that the proposed method can effectively reduce the influence of noises, and it has good ability in locating structural damages and quantifying damage degrees.

Diagnostic Value of ¹⁸F-FDG PET/CT in Comparison to Bone Scintigraphy, CT and ¹⁸F-FDG PET for the Detection of Bone Metastasis

Liu, Ning-Bo,Zhu, Lei,Li, Ming-Huan,Sun, Xiao-Rong,Hu, Man,Huo, Zong-Wei,Xu, Wen-Gui,Yu, Jin-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Purpose: To evaluate the diagnostic value of $^{18}F$-FDG PET/CT for detection of bone metastasis in comparison with the efficacies of $^{18}F$-FDG PET/CT, CT, $^{18}F$-FDG PET and conventional planar bone scintigraphy in a series of cancer patients. Methods: Five hundred and thirty patients who underwent both $^{18}F$-FDG PET/CT and bone scintigraphy within 1 month were retrospectively analyzed. The skeletal system was classified into 10 anatomic segments and interpreted blindly and separately. For each modality, the sensitivity, specificity, accuracy, PPV and NPV were calculated and the results were statistically analyzed. Results: Bone metastases were confirmed in 117 patients with 459 positive segments. On patient-based analysis, the sensitivity, specificity, accuracy, PPV and NPV of $^{18}F$-FDG PET/CT were significantly higher than bone scintigraphy, CT and $^{18}F$-FDG PET (P<0.05). On segment-based analysis, the sensitivity of CT, bone scintigraphy, $^{18}F$-FDG PET and $^{18}F$-FDG PET/CT were 70.4%, 89.5%, 89.1% and 97.8%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The overall specificity and accuracy of the four modalities were 89.1%, 91.8%, 90.3%, 98.2% and 90.3%, 90.9%, 89.8%, 98.0%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The PPV and NPV were 89.8%, 87.6%, 85.6%, 97.2% and 85.6%, 93.2%, 92.8%, 98.6%, respectively. Three hundred and twelve lesions or segments were presented as lytic or sclerotic changes on CT images at the corresponding sites of increased $^{18}F$-FDG uptake. In lytic or mixed lesions, the sensitivity of $^{18}F$-FDG PET/CT and $^{18}F$-FDG PET were better than bone scintigraphy, while in osteoblastic lesions bone scintigraphy had a similar performance with $^{18}F$-FDG PET/CT but better than $^{18}F$-FDG PET alone. Conclusion: Our data allow the conclusion that $^{18}F$-FDG PET/CT is superior to planar bone scintigraphy, CT or $^{18}F$-FDG PET in detecting bone metastasis. $^{18}F$-FDG PET/CT may enhance our diagnosis of tumor bone metastasis and provide more information for cancer treatment.

Study the Thermal Behaviours for Asphalt Concrete Mixed with Basic Oxygen Furnace Slags

( Huan-lin Luo ),( Deng-fong Lin ),( Show-ing Shieh ),( Yi-hong Liu ),( Yu-kai Wang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-

In Taiwan, the basic oxygen furnace (BOF) slags are characterized as porous material and with better heat insulation than that of the natural aggregates. The BOF slags have been applied to the pavement as parts of the natural aggregate replacement. The asphalt concrete (AC) with the BOF slags replacements were well recognized and performed. In this study, parts of natural aggregates were replaced by the BOF slags in the AC helping resolve the gradual shortage of the natural resources and save energy and reduce the produce of carbon. In this study, parts of the natural aggregates were replaced by the coarse and fine BOF slags in the construction of AC. Because the thermal insulation and heat absorption are different between BOF slags and natural aggregates, the infrared thermal conductivity measuring instrument was applied to obtain the surface temperatures presented as thermal infrared images in AC. Then, the infrared temperature distribution images were obtained by using the image transformation techniques. The results show that the thermal conductivity for the AC containing with fine BOF slag was larger than that for the coarse BOF slag. The BOG slag replacement in AC helped improve the thermal insulation in AC. Moreover, the temperature was more uniformly distributed for AC with the fine BOF slag than that for the coarse BOF slag replacement. However, the coarse BOF slag was better in the preserve of energy for AC than that of the fine BOF slag.

Effect of Monascus sp. as an Adjunct Starter on Physicochemical Properties and Proteolysis in Semi-hard Cheeses during Ripening

Huaning Yu,Zhenmin Liu,Feng Hang,Beihong Mo 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

Two kinds of semi-hard cheeses, with Monascus purpureus and without M. purpureus, were manufactured, and effects of M. purpureus on physicochemical properties and proteolysis were evaluated during 36 days of ripening. Addition of M. purpureus changed the microbial survival and showed no significant effect on physicochemical properties of the cheeses, including dry matter and pH. Regardless on the rind or in the core, the indices of proteolysis had no significant difference (p>0.05), whereas there were significant differences of total free amino acid (FAA) and individual FAA between cheeses; this indicated that M. purpureus had no significant effect on the primary proteolysis, but affected the content and ratio of individual FAAs during maturation. Electrophoretic analysis showed strong degradation of αs1-casein in the core and on the rind of cheeses, while β-casein was highly degraded on the rind but less in the core. Thus, Monascus spp. might have a potential application in the manufacture of cheeses.

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ‑GMP

Yu Ying,Huan Siqi,Wang Xiaodan,Yang Cong,Liu Dengyong 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.4

A sensitive electrochemical sensor for the selective detection of 5ʹ-guanylic acid (5ʹ-GMP) was prepared by combining sulfonated-multiwalled carbon nanotubes (SMWCNTs) and [Ru(bpy)2dpp]Cl2, which were dripped on the surface of a glass carbon electrode (GCE) immobilized with gold nanoparticles. The 5ʹ-GMP electrochemical biosensor was fabricated using [Ru(bpy)2dpp]2+/SMWCNTs/Au/GCE as working, Ag/AgCl as reference and Pt as auxiliary electrode connected by an electrochemical workstation. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed the sensor’s response current had the best peak shape and maximum peak when the pH of electrolyte was 3, scan speed of CV was in the range of 100 to 180 mV/s, and the enrichment time was in the range of 200 to 300 s. Under the optimum conditions, a linear analytical curve was obtained for 5ʹ-GMP concentrations in the range of 0.01 to 0.5 mmol L− 1, with a detection limit of 0.0014 mmol L− 1. The analytical results of the 5ʹ-GMP sensor were exhibited good consistent with the data from liquid chromatography. The sensor has good reproducibility, long-term stability and strong immunity to interference, and may be a powerful device for 5ʹ-GMP detection, with great advantages such as simple preparation and operation, low equipment cost.

Population Genetic Structure of an Endemic Cyprinid Fish, Ancherythroculter nigrocauda, in the Upper Reaches of the Yangtze River and its Implication for Conservation

Huan Zhang Liu,Yu Rong Zhu,Jian Wei Wang,De Qing Tan 한국유전학회 2005 Genes & Genomics Vol.27 No.4

Annual Financial Impact of Thyroidectomies for Nodular Thyroid Disease in China

Liu, Xiao-Yun,Zhu, Li-Jun,Cui, Dai,Wang, Zhi-Xiao,Chen, Huan-Huan,Duan, Yu,Shen, Mei-Ping,Zhang, Zhi-Hong,Wang, Xiao-Dong,Chen, Jia-Wei,Alexander, Erik Karl,Yang, Tao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

A large proportion of patients with thyroid nodules in China undergo thyroidectomy in order to get confirmatory histology diagnosis. The financial impact of this modality remains to be investigated. To evaluate rationality of performing thyroidectomy without a routine FNA preoperatively from the economic perspective, we conducted a retrospective, observational study of all archival thyroidectomies with records of cost per stay (CPS), cost per day (CPD) and length of stay (LOS) from 2008 to 2013 in the First Affiliated Hospital of Nanjing Medical University. We compared all the parameters between cancer and non-cancer thyroidectomies. We recruited 6, 140 thyroidectomies with valid records of CPS, CPD and LOS in this period. The CPS of cancer thyroidectomy was significantly higher than non-cancer thyroidectomy. The percentage of cancer thyroidectomy increased from 26.5% to 41.6%. The percentage of annual cost of cancer thyroidectomies rose from 30.2% to 45.2%. The LOS for cancer and non-cancer thyroidectomy decreased while the CPD increased in the past six years. The estimated national cost in 2012 for all thyroidectomies would be USD 1.86 billion with USD 1.09 billion for non-cancer thyroidectomies. We have witnessed great improvement in the healthcare for patients with thyroid nodules in China. However, given limited healthcare resources, currently thyroid FNA for more precise preoperative diagnosis may help to curb the rapidly increasing demand in healthcare costs in the future for nodular thyroid disease in China.

Preparation of moso bamboo columnar activated carbon with high adsorption property via polyacrylamide@asphalt adhesives and steam activation

Liu Huan,Miao Yu,Tian Huayu,Chen Yishan,Wang Enfu,Huang Jingda,Zhang Wenbiao 한국탄소학회 2024 Carbon Letters Vol.34 No.3

Moso bamboo, as a kind of renewable functional material, exhibits outstanding development potential. It is promising to prepare activated carbon with good mechanical strength and high specific surface area using moso bamboo as raw material. In this work, we employed a hydraulic extruder to extrude the bamboo charcoal and the adhesive to obtain the moso bamboo activated carbon, and improved the specific surface area of the columnar activated carbon through high-temperature water vapor activation. Through the catalytic role of the water vapor activation process, the formation and expansion of the pores were promoted and the internal pores were greatly increased. The obtained columnar activated carbon shows excellent mechanical strength (93%) and high specific surface area (791.54 m2/g). Polyacrylamide@asphalt is one of the most effective adhesives in the high-temperature water vapor activation. The average pore size (22.99 nm) and pore volume (0.36 cm3/g) of the prepared columnar activated carbon showed a high mesoporous ratio (83%). Based on the excellent pore structure brought by the activation process, the adsorption capacity of iodine (1135.75 mg/g), methylene blue (230 mg/g) and carbon tetrachloride (64.03 mg/g) were greatly improved. The resultant moso bamboo columnar activated carbon with high specific surface area, excellent mechanical properties, and outstanding adsorption capacity possesses a wide range of industrial applications and environmental protection potential.