흰쥐에서 급성 스트레스 유발 불안반응의 해소에 대한 신경교세포 말초성 벤조다이아제핀 수용체의 매개작용

김영욱,박형배,이종범,김진성,정성덕,하정희,송창진 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적 : 말초성 벤조다이아제핀 수용체가 스트레스로 유발된 불안반응에서의 불안해소작용을 알아보기 위해 흰쥐에 급성 부동화(immobilization) 스트레스를 부하하여 유도된 불안반응에서 뇌조직중의 말초성 벤조다이아제핀 수용체의 매개작용을 검색하고자 본 연구를 시행하였다. 방 법 : 흰쥐에 급성 부동화 스트레스를 가한 후 실험동물의 불안도를 elevated plus maze를 통하여 검정하고 말초성 벤조다이아제핀 수용체의 변화를 수용체 결합반응을 통하여 검색하여 대조군과 비교했고, 말초성 벤조다이아제핀 수용체 효현제 및 길항제의 투여에 의한 실험동물의 불안도의 변화 양상도 동일한 검사를 통하여 검정하였다. 결 과 : 급성 부동화 스트레스에 부하되었던 흰쥐는 불안반응을 나타내었으며, 실험동물에 선택적인 중추성 벤조다이아제핀 수용체 효현제인 clonazepam을 투여하여 스트레스로 인한 불안반응을 감약시킨 후 급성 부동화 스트레스를 가했을 때는 불안반응이 감약되었으며, 후구(olfactory bulb)의 말초성 벤조다이아제핀 수용체의 상향조절도 관찰되지 않았다. 말초성 벤조다이아제핀 수용체 효현제인 Ro5-4864을 투여 후 약한 불안 해소 작용을 나타내었으며, 신경활성 스테로이드의 전구물질인 progesterone을 흰쥐에게 투여한 후 이 실험군의 통로로의 진입율 및 개방통로에서의 체류율은 대조군에 비하여 유의하게 증가되었다. 결 론 : 말초성 벤조다이아제핀 수용체는 급성 스트레스 부하시 야기되는 불안반응에서 내인성 GABA성 신경전달의 조절자로 알려진 신경활성 스테로이드의 합성을 증가시킴으로써 불안반응을 해소하는 기능을 가진다. Objectives : Peripheral benzodiazepine receptor has been suggested to be associated with the relief of anxiety response induced by stresses. This study was designed to observe the anxiolytic activity of peripheral benzodiazepine receptor. Methods : Male Sprague-Dawley rats, weighing 200-250g were forced to suffer an immobilization stress for 2 hours. The level of anxiety by immobilization was performed by an elevated plus maze and was evaluated by the number of [³H]Ro5-4864 binding sites in the olfactory bulb. Results : Saturation experiments followed by scatchard analyses of the results showed that the density of peripheral benzodiazepine receptor increased and the affinity of the peripheral benzodiazepine receptor remained unchanged. It was found that there was no significant change in the cerebral cortex. Pretreatment with clonazepam, a central benzodiazepine receptor agonist, before an immobilization stress abolished the anxious response on the performance of plus maze. In this group, upregulation of peripheral benzodiazepine receptor of olfactory bulb was not observed. Ro5-4864, a peripheral benzodiazepine receptor agonist, elicited an increase of anxiolytic response on the performance of plus maze. Progesterone, a precursor of neuroactive steroid, also increased anxiolytic response on the performance of pius maze. Pretreatment with PK11195, a peripheral benzodiazepine receptor antagonist, abolished the anxiolytic effect of progesterone. Conclusions : From these results, it could be concluded that peripheral benzodiazepine receptor is closely associated with the relief of acute stress induced anxiety response via an increase of synthesis of neuroactive steroid.

Case Reports : A Case of Rheumatoid Arthritis Presenting as an Intra-articular Mass of the Wrist Joint in a Patient with Chronic Monoarthritis

( Eun Jung Park ),( Young Uck Kim ),( Jiyoung Kim ),( Chang Lim Hyun ),( Kyung Ryeol Lee ),( Jinseok Kim1 ) 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.5

Rheumatoid arthritis (RA) mainly affects polyarticular joints and is characterized by inflammation of the synovial membrane leading to joint destruction. We report on an unusual case of RA presenting as an intra-articular mass invading bone of the wrist joint in a patient with chronic monoarthritis. A 43-year-old man presented with left wrist joint pain and swelling lasting several years. A plain radiograph showed a non-specific osteolytic lesion in the distal ulna but a magnetic resonance image demonstrated an intra-articular irregular mass-like lesion with eccentric bone erosion the distal radioulnar joint. Synovial biopsy detected hyperplasia of the synovial lining cell layer and finger-like protrusions of inflamed and edematous fibrovascular stroma containing dense inflammatory infiltrates, mainly plasma cells, B cells, and CD4+ T cells. Rheumatoid factor and anti-citrullinated protein antibody were highly positive. The patient was diagnosed with RA and treated with disease-modifying anti-rheumatic drugs, showing a good response on further follow-up. (J Rheum Dis 2015;22:298-302)

통조림罐의 內面腐蝕에 미치는 有機酸의 影響

孫泰華,崔鍾旭,權榮澤,金聲達 경북대학교 1981 새마을 硏究論叢 Vol.1 No.-

This experiment was carried out to research the effect of kinds and concentration of organic acid food component on the internal can corrosion, stored at room temperature (25℃) and low temperature (0℃). The criterion for the degree of the internal can corrosion was tin contents in the pure acid solution. The result of this research can be summarized as followes. The intension which effect the rate of the tin dissolution was furmaric>malic>citric, were respectively. The rate of the dissolution were increased rapidly at 30days after treatment, and the higher concentration of malic acid, the more dissolved of tin from the can body. The amounts of tin dissolved from can body at room temperature were slightly stronger than low temperature.

급성충수돌기염으로 발현된 방선균증 2예

조진원,송진영,김창억,민영훈,최창순,우흥정,현인규,김종현,유기철,채승완,손진희 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

The Actinomyces species are normal flora of oral cavity and upper intestine characterized as gram-positive, non-spore-forming anaerobic or mjcroaerophilic rods. Actinomycosis is a chronic suppurative and granulomatous disease characterized by abscess, sinus, abundant granulation and mass. The appendix is the commonly involved site among abdominal actinomycosis. Abdominal actinomycosis is often difficult to diagnose before operation because of no reliable clinical features and infrequency of the disease. We report two cases of abdominal actinomycosis which were manifested as acute appendicitis. Those were detected Incidentally tv the pathologist after routine appendectomy. Both patients were successfully treated with appendectomy followed by proper antibiotics use.

인슐린 자가항체 및 인슐린 수용체 자가항체와 연관된 자가면역성 저혈당증 1예

김미진,심명숙,김문규,이연,김영욱,신영구,정춘희 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

Autoimmune hypoglycemia is caused by the interaction of endogenous antibodies with insulin or insulin receptor. Insulin autoantibody(IA) complexes are present in most patients who receive insulin therapy, but have also been reported in nondiabetic patients with autoimmune disease. IA may also be detected in 1% to 8% of healthy subjects with no history of diabetes or autoimmune disease. Insulin receptor autoantibody(IR-A) complexes are associated with the inhibition of insulin binding to insulin receptors, accelerated receptor degradation, receptor downregulation, and extreme insulin resistance and hyperglycemia, but can also lead to refractory hypoglycemia. We experienced a case of autoimmune hypoglycemia with IA and IR-A without previous insulin injection therapy or evidence of insulinoma. Therefore we started glucocorticoid therapy, and the hypoglycemic symptom improved. Herein, we present this case with a review of the literatures.

Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3′,4′-hexamethoxyflavone via NF-κB inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage

KIM, MIN JEONG,LEE, HYE HYEON,JEONG, JIN WOO,SEO, MIN JEONG,KANG, BYOUNG WON,PARK, JEONG UCK,KIM, KYOUNG-SOOK,CHO, YOUNG-SU,SEO, KWON-IL,KIM, GI-YOUNG,KIM, JUNG-IN,CHOI, YUNG HYUN,JEONG, YONG KEE SPANDIDOS PUBLICATIONS 2014 MOLECULAR MEDICINE REPORTS Vol.9 No.4

S-223 : Relations between Chest Pain and Psychological Characteristics with Non-Coronary Artery Disease

( Young Uck Kim ),( Joon Hyouk Choi ),( Song Yi Kim ),( Ki Seok Kim ),( Seung Jae Joo ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Background and Objective: One of the chief complaints of outpatients who visit to cardiologists is chest pain. However, when they are examined, most of them do not have coronary artery disease (CAD). There are few cardiologists who carry out psychological tests in order to find out why the patients are diagnosed as non-CAD. The purposes of this study are 1) to find out psychological characteristics of the patients having CAD and non-CAD and 2) to see the relations between the intensity of chest pain and the psychological characteristics. Subjects and Methods: 108 patients with chest pain were enrolled. They answered questionnaires: numerical rating scale (NRS) of pain and symptom check list-minor psychiatric disorders (SCL-MPD). We divided them into two groups. Group 1 consisted of low probability of acute coronary syndrome (ACS) and Group 2 consisted of moderate to severe probability. Group 1 had a treadmill test, single-photon emission computed tomography (SPECT), or coronary artery MDCT. Then, coronary angiography (CAG) was performed to the patients who showed abnormal results. Group 2 had CAG directly. Results: 19 patients (17.6%) were diagnosed as CAD. 10 patients (9.3%) underwent revascularization. The score of somatization was significantly higher in non-CAD group than CAD group (13 [9-20] vs. 9 [6-13], p=0.032). The score of anxiety and NRS were correlated in non-CAD group (r=0.246, p=0.020). The score of depression and NRS were inversely correlated in CAD group (r=-0.504, p=0.028). In multivariable analysis, a high score of anxiety (≥11 score, ≥75 percentiles) was the only meaningful predictor of severe chest pain (≥6 score, ≥75 percentiles) in all the patients (odds ratio 8.962, p=0.011, 95% confidence interval 1.665-48.252). Conclusion: The score of somatization was higher in non-CAD group than CAD group. The intensity of chest pain and the score of anxiety were correlated.

Integrated Cell Culture-PCR Detection of Enteroviruses and Reoviruses in Water Sources in Gyeonggi-do

Kim, Kyung-A,Kim, Jong-Chan,Ko, Hoan-Uck,Lee, Jung-Bock,Kim, Young-Sug,Park, Yong-Bae,Lee, Myung-Jin,Kim, Myung-Gill,Kim, Jae-Kwan,Park, Eun-Mi 대한미생물학회 2008 Journal of Bacteriology and Virology Vol.38 No.2

The integrated cell culture-PCR (ICC-PCR) method has been suggested as an improved method for detection of viruses in water environments. We tested 57 source waters including finished water samples in Gyeonggi-do for enteric viral contamination using total culturable virus assay (TCVA) using BGMK cells and ICC-PCR. Nineteen of the 57 source water samples (33.3%) exhibited the cytopathic effect (CPE) on BGMK cells and no finished water did exhibited CPE. Nineteen samples (33.3%) of the 57 were positive for reoviruses. For the enteroviruses, only 3 samples (5.3%) of the 57 samples showed positive results. By using ICC-PCR method, 202 flasks from source water samples were positive for enteroviruses and reoviruses. Three samples from source water were positive for both viruses. However, any flasks tested was not co-infected with two types of viruses. While the enteric viral frequencies in TCVA and ICC-PCR were similar, the viral frequency for reoviruses at first passage in two type of method was higher in ICC-PCR (94.7%) than TCVA (56.9%).

일개 대학병원에서 일년간 분리된 반코마이신 내성 장구균의 임상 분자역학적 연구

송진영,김창억,김성욱,우흥정,김미란,이규만,이란,장미화,정희진,김우주 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.4

목적 : 한강성심병원에서는 2000년 3월 반코마이신내성 장구균이 처음 분리되어, 원내전파를 막기 위한 노력을 하였으나, 분리가 지속되었다. 따라서 이에 대한 좀더 자세하고 객관적인 자료를 얻기 위해 본원에서 분리된 반코마이신내성 장구균에 대한 임상분자 역학적 연구를 시행하게 되었다. 방법 : 2000년 3월부터 2001년 2월까지 한림대학교 한강성심병원에서 분리된 장구균을 대상으로 디스크 확산법, 최소발육억제 농도등의 측정을 통해 반코마이신 내성여부를 확인하였으며, 반코마이신내성 장구균을 대상으로 PFGE를 시행하여 형별 분석을 하였다. 결과 : 분리된 균주는 총 32균주로 모두 E. faecium이었다. 항생제 내성 검사 결과 모두 Van A형이었다. PFGE 결과 1a형이 8검체, 1b형이 5검체, 2a형이 4검체, 2b형이 4검체, 3a형이 2검체, 3b형이 5검체였고, 그 외 4, 5, 6형이 각각 1검체씩이었다. 총 32균주 중 56%(18/32)의 균주가 같은 시기, 같은 병실에서 동일한 PFGE 형을 보이면서 분리되었다. 결론 : 반코마이신내성 장구균의 PFGE 분석 결과 여러 종류의 반코마이신내성 장구균이 유행하였음을 알 수 있다. 그러나 같은 기간, 같은 병실에서 분리된 균주의 PFGE 형이 같은 것이 상당수 있었다. 이는 반코마이신내성 장구균의 원내 전파가 있었음을 의미한다. 따라서 반코마이신내성 장구균의 전파의 발생 및 전파 방지를 위해서는 보다 효과적인 감염관리 활동이 필요할 것으로 생각된다. Background: Vancomycin-resistant enterococci(VRE) have been a rising problem worldwide. In March 2000, VRE was first isolated from a patient in Hangang Sacred Heart hospital. Although efforts to prevent transmission of VRE were performed, isolations continued. So molecular epidemiological study of VRE was done. Method : The minimal inhibitory concentration(MIC) to vancomycin and teicoplanin, and pulsed field gel electrophoresis(PFGE) pattern of VRE isolated from March 2000 to February 2001 were evaluated. Results : 32 VRE were isolated. All of the VRE were Enterococcus faecium and showed Van A resistance phenotype. We found out that a significant number of VRE isolated during same period and in same room, were same PFGE patterns. (18 cases/ 32 isolates= 56%) Conclusion: This study demonstrated the spread of VRE of same PFGE patterns. It suggests the nosocomial spreads of VRE.

페로니켈 슬래그 잔골재를 사용한 모르타르의 혼화재료 치환율에 따른 유동성 및 강도특성

김영욱(Kim, Young-Uck),김택수(Kim, Taek-Soo),서동명(Seo, Dong-Myoung),김성진(Kim, Sung-Jin),한지민(Han, Ji-Min),최세진(Choi, Se-Jin) 한국콘크리트학회 2016 한국콘크리트학회 학술대회 논문집 Vol.28 No.1